LOCUS       EU294169               12235 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Shigella dysenteriae O antigen gene cluster, complete sequence.
ACCESSION   EU294169
VERSION     EU294169.1
KEYWORDS    .
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12235)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 12235)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..12235
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:622"
                     /note="type: 12"
     gene            1137..2222
                     /gene="rmlB"
     CDS             1137..2222
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ACA24804.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDPERYIFEHADICDAAAMARIFAQHQPHAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LANAKWVENVKSGAYQSWIEQNYEGRQ"
     misc_feature    1137..10788
                     /note="O antigen gene cluster"
     gene            2222..3121
                     /gene="rmlD"
     CDS             2222..3121
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ACA24805.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGMYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPATAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3179..4057
                     /gene="rmlA"
     CDS             3179..4057
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ACA24806.1"
                     /translation="MKTRKGIILAGGAGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTSQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKALAEPL
                     KKNAYGQYLIKMIKGY"
     gene            4062..4610
                     /gene="rmlC"
     CDS             4062..4610
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ACA24807.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFVYKTTNYYHPESDRGIRWDDPYIGIKWENE
                     YNVRLSDKDKKNPTMKDVFLFM"
     gene            4626..5663
                     /gene="wfgB"
     CDS             4626..5663
                     /gene="wfgB"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24808.1"
                     /translation="MKKNIIAYYLPQYHEVKENNEWWGKGFTEWTALKKAKKYFPSQK
                     IRLPTDFLGYYDLTESRIIEKQFELAEENGVSGFCLWTYWFGNGEKILEKPLTLILEN
                     KLNVRYCVAWANHSWYNKSKGLLLKEQKYLGEKDYTDFFYYLLPHFKSDNYLKKDNKP
                     IVTIFDPNSIPDLFLFISLWNNLAKENGYEGIYFIGDFTSYNSNYVGVFDGYLDSRVM
                     YRKRTFIQKVREKLVRKHRVKFLGPIRYNYEKMISSLWHNQTKDIKEIPIIFSGWDTT
                     IRHGKQGVFYSDFSEHSFEVNVKNAINYNPQQDIVFLKSWNEWAEGNTVEPDTIFSDK
                     LLRIISKYNKY"
     gene            5663..6874
                     /gene="wzx"
     CDS             5663..6874
                     /gene="wzx"
                     /note="O antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ACA24809.1"
                     /translation="MRKNIFYLLLVQLSNYIFPLITLPYLVRTLGVDKFGYLMLGQAL
                     IQYFILMTDYGFNFSATKKIATSTSIDERNEVYTETLNAKMILVTLCLFLMLIIINII
                     PMFHSISIVCIILFIGVLGNIFFPVYLFQGMEKMKSIAWVTVLARTLMLLSVFLFVKD
                     NNDINAAALAFSIALIVPGMMSIYLIRKNHIVAYKGFSFSKAFIAIKASTPLFISQIA
                     ITFYTTFNSILIGHVFNARQVGIYSAADKLRGAVQSLFIPIQQVVFPRINVERGNIKE
                     KLKFYGSLFILFSLIICVSIFLIGDKIVILYFGTQYAESANLFKWMSVLIFIVSVAIV
                     FSQWGMITLGREKMLTKIYIVGALLHCTYAPFFTKNFGLFGTLTAVILTELTITVVMG
                     VFLYKIIKRHK"
     gene            6877..7869
                     /gene="wfgC"
     CDS             6877..7869
                     /gene="wfgC"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24810.1"
                     /translation="MKSNTIDIVIPWVDSSDPKWQEDYMKFACEQRNVDANIHRYRDN
                     GLLKFWFRGIEKNAPWIRKVHFITYGHVPAWLKQDHPKLHIVKRSDYIPQKYLPTFNS
                     HVIELNLHKIDGLSDKFIYYNDDTFLINPVDESFYFKNGKPRDCAILNAVQPDGLSHV
                     ILNNLSIINKHFCKKQVIRKNILKWINVKYGAALSRTICLMPWPKFVGIKDTHLPIPY
                     LKSTFDKLWELEPEILNATNRSKFRQNSDINQYLFRYWQLVSGEFECSNTMASSAYLD
                     IGKDSIIKIRKELFCSRNKLVVINDADTLEFDSKTKKIQEEFAKIFPFKSTFEI"
     gene            7882..8955
                     /gene="wzy"
     CDS             7882..8955
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ACA24811.1"
                     /translation="MLFTYASYLCILVFSTSVAFCYEHSKSNFKVLLWSVLFLSLWIP
                     AAIRFGVGADYFRYLSMVESVRLGYISTEVGFYFINYFVVWFDLNPQWSIAISSFIVI
                     FLSLKAIPRNYFSVSVFVLVCTFYLPSFSLVRQAIAIAFIAYAVKNYIDGNKKNYIIF
                     ILIGSLFHLSALLLVPIYFIARFRVRSSILFLGILIISLLLFLGSGLNALLNSEFFLQ
                     SKYGYYATSDFIQDAKIGSGVGVFIKMLLPLIFITNAKKILKTNQNLNILLWMSVGCV
                     LANVCSMKIHIFNRLADIFLFVNFMVTSYIINSLEKNVIKQLSSMAIIIVFIVFYCRT
                     IQANYNDDLGGIGISPYKTILSK"
     gene            8960..9721
                     /gene="wfgD"
     CDS             8960..9721
                     /gene="wfgD"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24812.1"
                     /translation="MDDYLVSIIMPSYNAEHTISASISSVLKQTYANWELLVCDDDSS
                     DNTRFKVLEFSDSRIKLLTNEYAKGAAGARNTALKYASGRFIAFLDSDDIWIANKLEM
                     QISMMLKNNISFMYGNYEIINNNSIVGKFVAPQKITYNKLLKNCGIGCLTVVLDRTLL
                     NPFSFPFVHKEDYYLWLSILKDNNISAINCGFICSKYRLSQSSISSNKFKELKRQWDV
                     LGDFVENPLARIYYLLNYIVIGIKKHAFDYKNGKK"
     gene            9718..10788
                     /gene="wfgE"
     CDS             9718..10788
                     /gene="wfgE"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24813.1"
                     /translation="MKKIAFVIADITFVGGIERVNTILANKFISEGYSVEIISLYKTN
                     LKINYELDNHIKISFVNDGSYLGEPGSFGRLKKHIGSQFKLFKAIKKSNADFYIINTF
                     PMAFLSFFSLFSKKKYVVIEHVHYDYYSAIVRALRNLLYRFFYKVICINKNDLYKFSK
                     HLDNVVKISNPLSFSCSKVADLSAKKIMAVGRLEHQKGFDLLIDIFAKVNKSNPGWEL
                     HIYGVGTCEKFLTDKINKHGLNNVKLMGSVDHIQQYYPKYSIFAFSSRFEGFGMVLLE
                     AMECGLPCISFDCPTGPSEILGDGEYGILVENGNLIKFSAELANLMSDEEKKIKFSIL
                     SKSRAQEFNIDKIFDEWRKLIS"
BASE COUNT     3745 a   2058 c   2603 g   3829 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgagcagcg cgtgaagcgt
      121 caactgttag cggaagttca gtccatctgc ccgccgggcg tgactattat gaacgtgcgt
      181 cagggcgaac ctttaggttt gggccactcc attttatgtg cacgacctgc cattggtgac
      241 aacccatttg tcgtggtact gccagacgtt gtgatcgatg acgccagcgc cgacccgctg
      301 cgctacaacc ttgcagccat gattgcacga ttcaacgaaa cgggtcgtag ccaggtgctg
      361 gcaaaacgca tgccggggga cctctctgaa tactctgtca tccagaccaa agagccgctg
      421 gatcgtgaag gcaaagtcag ccgcattgtt gaatttatcg aaaaaccgga tcagccgcag
      481 acgttggagt cagacatcat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcacgcagcc aggtgcatgg gggcgtattc agctgactga tgccattgcc
      601 gaactggcga aaaaacagtc cgttgatgcc atgctgatga caggtgacag ctacgactgc
      661 ggtaaaaaaa tgggttatat gcaggcattt gtgaagtatg gactacgcaa cctgaaagaa
      721 ggggcgaagt tccgtaaagg cattgagaag ctgttaagcg aataatgaaa atctgaccgg
      781 atgtaacggt tgataagaaa attataacgg cagtgaagat tcgtggcgaa agtaatttgt
      841 tacgaatttt cctgccgttg ttttatataa acaatcagaa taacaacgag ttagcaatag
      901 gattttagtc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtttgaa tttttcgggt ttagcgcgag tgggtaacgc tcgtcacatc gtagacatga
     1021 atgcagtgct ctggtagctg taaagccagg ggcggtagcg tgcattaata cctctattaa
     1081 tcaaactaag agccgcttat ttcacagcat gctctgaagt aatatggaat aaaagagtga
     1141 agatacttgt tactggtggc gcaggattta ttggttctgc tgtagttcgt cacattataa
     1201 ataatacgca ggatagtgtt gttaatgtcg ataaattaac gtacgccgga aacctggaat
     1261 cacttgctga tgtttctgat cctgaacgct atatttttga acatgcggat atttgcgatg
     1321 ctgctgcaat ggcacggatt tttgctcagc atcagccgca tgcagtgatg cacttggctg
     1381 ctgaaagcca tgttgaccgt tcaattacag gtcctgcggc atttattgaa accaatattg
     1441 ttggtactta tgtccttttg gaagccgctc gcaattactg gtctgctctt gatagcgaca
     1501 agaaaaatag cttccgtttt catcatattt ctactgacga agtctatggt gatttgcctc
     1561 atcctgacga ggtaaataat aaagaacaat tacccctctt tactgagacg acagcttacg
     1621 cgccaagtag cccatattct gcttcgaaag catcaagcga tcatttagtc cgcgcttgga
     1681 aacgtaccta tggtttaccg accattgtga ctaattgttc gaataactac ggtccttatc
     1741 actttccgga aaaattgatt ccactagtaa ttcttaatgc tctggaaggt aaggcattac
     1801 ctatttatgg caaaggggat caaattcgtg actggctgta tgttgaagat catgcgcgtg
     1861 cgttatatac cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacaca
     1921 acgaaaagaa aaacatcgat gtagtgctca ctatttgtga tttgttggat gagattgtac
     1981 cgaaagagaa atcttatcgt gagcaaatta cttatgttgc cgaccgcccg ggacatgatc
     2041 gccgttatgc gattgatgct gagaagatta gccgcgaatt gggttggaaa ccacaggaaa
     2101 cgtttgagag cgggattcgt aaaacggtgg aatggtacct ggctaatgca aaatgggtcg
     2161 agaatgtgaa aagtggtgcc tatcaatcgt ggattgaaca gaactatgag ggccgccagt
     2221 aatgaatatt ctccttttcg gcaaaacagg gcaggtaggt tgggaactac agcgtgctct
     2281 ggcacctttg ggtaatttga ttgctcttga tgttcactcc actgattatt gtggtgattt
     2341 tagtaatcct gaaggtgtgg ctgaaaccgt caaaaaaatt cgccctgatg taattgttaa
     2401 tgctgcggct cataccgcag tagataaggc tgagtcagaa cccgaatttg cacaattact
     2461 caatgcgacc agcgttgaat caattgccaa agcggctaat gaagttgggg cctgggtaat
     2521 tcattactca actgactacg tatttccggg aaccggtgaa ataccatggc tggagacgga
     2581 tgcaaccgca ccgctaaatg tttacggtga aaccaagtta gccggagaaa aagcgttaca
     2641 ggaacattgc gcgaagcatc ttattttccg taccagctgg gtatacgcag gtaaaggaaa
     2701 taacttcgcc aaaacgatgt tgcgtctggc aaaagagcgc gaagaactgg ctgtgattaa
     2761 tgatcaattt ggtgcgccaa caggtgctga gctgctggca gattgtacgg cacatgctat
     2821 tcgtgtggca ctgaataaac cagaagtcgc aggcatgtac catctggtag ccagtggtac
     2881 cacaacctgg cacgattatg ctgcgctggt ttttgaagag gcgcgcaaag caggtattcc
     2941 ccttgcactc aacaagctca acgcagtacc agcaacagcc tatcctacac cagctcgtcg
     3001 tccacataac tctcgcctta atacagaaaa atttcagcag aactttgcgc ttgtcttgcc
     3061 tgactggcag gttggcgtga aacgaatgct taacgaatta tttacgacta cagcaattta
     3121 atagtttttg catcttgttc gtgatggtgg aacaagatga attaaaagga atgatgaaat
     3181 gaaaacgcgt aaaggtatta ttttagcggg gggggctggt actcgtcttt atcctgtgac
     3241 tatggctgta agcaaacagc tgttaccgat ttatgataaa ccgatgatct attacccgct
     3301 ttctacactg atgttagcgg gtattcgcga tattctgatt atcagtacgt cacaggatac
     3361 tcctcgtttt caacaactgc tgggtgatgg gagccagtgg gggctaaatc ttcagtacaa
     3421 agtgcaaccg agtccagatg gtcttgcgca ggcatttatc atcggtgaag agtttatcgg
     3481 tggtgatgat tgtgctctgg ttctcggtga taatatcttt tacggtcacg atctgccgaa
     3541 gttaatggat gtcgctgtca acaaagaaag tggtgcaacg gtatttgcct atcacgttaa
     3601 tgatcctgaa cgctacggtg ttgttgagtt tgataaaaac ggtacggcaa tcagcctgga
     3661 agaaaaaccg ctacaaccaa aaagtaatta tgcggtaacc gggctttatt tctatgataa
     3721 cgacgttgtc gaaatggcga aaaaccttaa gccttctgcc cgtggtgaac tagaaattac
     3781 cgatattaac cgtatttata tggaacaggg gcgtttatcc gttgccatga tggggcgtgg
     3841 ttatgcatgg ctggatacgg ggacacatca gagtcttatt gaagcaagca acttcattgc
     3901 caccattgaa gagcgccagg gactaaaggt ttcctgccca gaagaaattg cttaccgtaa
     3961 agggtttatt gatgctgagc aggtgaaagc attagctgaa cctctgaaaa aaaatgctta
     4021 tggtcagtac ctgataaaaa tgattaaagg ttattaataa aatgaatgtt attaaaacag
     4081 aaatcccaga tgtattgatt tttgaaccga aagtttttgg tgatgagcgt ggtttctttt
     4141 ttgagagctt taaccagaag gtttttgagg aagctgtagg ccgcaaagtt gaatttgttc
     4201 aggataacca ttcgaagtct agtaaaggcg ttttacgcgg actgcattat cagttagaac
     4261 cttatgcgca aggaaaattg gtacgctgtg ttgttggtga ggtttttgat gttgcggttg
     4321 atattcgtaa atcgtcccct acttttggca aatgggttgg ggtgaatttg tctgctgaga
     4381 ataagcgcca gttgtggata cctgaaggat ttgcgcatgg atttttggtg ctgagtgaga
     4441 ctgcggagtt tgtttataag acaactaatt attatcatcc ggaaagtgac agaggtatta
     4501 gatgggatga cccctatata ggaattaaat gggagaatga gtacaatgta agattgtcag
     4561 ataaagataa aaaaaatcca acaatgaaag acgttttttt gtttatgtaa gtaataggct
     4621 tttatatgaa aaaaaatatt atcgcatatt atcttcctca atatcatgag gttaaagaaa
     4681 ataatgagtg gtgggggaaa ggatttactg aatggacagc tttaaaaaaa gcaaagaaat
     4741 atttcccttc tcaaaaaata agattgccta ctgattttct tggatattat gatttgacag
     4801 aatcacgtat tatcgagaag cagtttgaac tagctgaaga aaatggtgta agtgggtttt
     4861 gtctatggac atattggttc ggtaatggag aaaaaatttt agaaaaacct cttactctta
     4921 ttcttgaaaa caaacttaat gttcgatact gtgtggcatg ggcaaaccac tcttggtaca
     4981 ataaatcaaa agggctactt ttaaaagagc aaaaatacct tggagaaaaa gattatactg
     5041 atttcttcta ttacttactt cctcatttta agtcagataa ttatctaaaa aaagataata
     5101 agccaattgt aacaatcttt gatcctaatt ccataccaga tttgtttttg tttatatccc
     5161 tttggaataa tcttgcaaaa gagaacggat acgagggtat ctattttatc ggggatttca
     5221 catcgtataa ttcaaattat gtaggagtat ttgatggtta tttggattct agagtaatgt
     5281 acaggaagag aacctttatt caaaaggtga gggagaaatt agttcgaaag catagagtga
     5341 aatttttggg gcctataagg tataattatg aaaaaatgat aagctcatta tggcacaatc
     5401 agacaaagga tataaaagag attccaatta tattttctgg atgggataca acgatacgac
     5461 atggaaaaca gggggttttt tatagtgatt tctctgagca ttcgtttgag gttaatgtta
     5521 aaaatgctat aaattataac ccacaacaag atattgtttt cttaaaatca tggaatgaat
     5581 gggctgaggg aaatacagtt gaaccagaca ctatattctc agataaatta cttagaataa
     5641 tttcaaaata caataaatac tgatgagaaa aaatatattt tatttactgt tggtgcagtt
     5701 atctaactat attttcccac tgattacatt accatatctt gtcagaactt taggagtaga
     5761 taaatttgga tatcttatgc ttggtcaagc attgattcaa tactttattt tgatgactga
     5821 ttacggtttt aatttcagtg caacaaaaaa aatagctaca tcaacaagta ttgatgaaag
     5881 aaatgaagtc tacacagaga cattaaatgc caaaatgata ttggtgacat tatgtttatt
     5941 cttgatgtta atcattatta atatcatacc aatgtttcac tctatatcta tagtttgcat
     6001 aatacttttt attggggttc taggtaatat tttttttcca gtctatctgt ttcaaggtat
     6061 ggagaaaatg aaaagtattg catgggttac tgtactagca aggaccttga tgttgttatc
     6121 tgtctttctg tttgtaaaag ataataatga tatcaatgct gctgcactag ctttttctat
     6181 tgcgcttatc gttccaggga tgatgtcaat ttatctaata cgaaaaaacc acattgttgc
     6241 ttataaaggg ttttcttttt caaaagcctt tatcgctatt aaagcaagta cacctttgtt
     6301 tatttctcaa atcgctataa cattttatac tacttttaat tcgatactta tagggcatgt
     6361 atttaatgca aggcaagtag gtatttattc tgcagcagac aagttaagag gcgcggttca
     6421 atctttattt attcctatac aacaggttgt gtttccgaga ataaatgttg agcgtggaaa
     6481 tataaaggaa aaactaaagt tttacggtag tctttttatt ttattttcat taattatatg
     6541 tgtgtcgata tttttaattg gagataagat tgttatttta tacttcggaa ctcagtatgc
     6601 tgagtcagct aatttattta aatggatgtc agtccttatt tttattgtct ctgtcgctat
     6661 tgtcttttca caatggggaa tgataacttt aggtagagaa aaaatgttaa caaaaattta
     6721 tattgtcggt gccttattgc attgcacgta tgctcctttc ttcactaaaa atttcgggct
     6781 ttttggtacg ttaacggcag taatattgac agagttaaca attaccgtag ttatgggcgt
     6841 gtttttgtac aagataatta aaaggcataa ataaaaatga aaagcaacac aattgacatt
     6901 gtaattccat gggtagatag ttctgatcct aaatggcaag aggattatat gaagtttgca
     6961 tgtgaacaaa gaaatgttga tgcaaatatt catcgttata gagataatgg gttattaaaa
     7021 ttctggtttc gtggcattga aaaaaatgcc ccttggatta ggaaagttca ttttattaca
     7081 tatggacatg ttccggcgtg gcttaagcaa gatcacccta aattacatat tgtaaagcgc
     7141 tccgactata taccccaaaa atacctacca acatttaata gccatgttat tgaattaaat
     7201 ttgcataaaa tagatggatt gagtgataag ttcatctatt ataatgacga tacatttttg
     7261 attaatccag tagatgaaag tttttacttc aaaaatggaa agcctcgaga ttgtgcaatc
     7321 ctgaacgcag ttcaacctga tgggctttca catgttattt taaataatct ttcaattatc
     7381 aataagcatt tttgtaaaaa acaggtgatc agaaaaaata ttttaaaatg gataaatgtt
     7441 aaatatggtg cggcgttaag tagaacgata tgtctaatgc cttggccgaa gtttgtaggt
     7501 atcaaagata ctcatcttcc aattccttat cttaagtcaa catttgataa attatgggag
     7561 ctcgagccag aaatattaaa tgctacaaat cggtcaaaat ttagacagaa ttcagatatt
     7621 aatcaatatc tctttagata ttggcagtta gtttcagggg agtttgaatg ctctaataca
     7681 atggcatctt cagcctattt agatataggt aaggatagta ttataaaaat tcgaaaggaa
     7741 ctattttgtt caagaaataa acttgttgtt ataaatgatg ctgatacttt ggaattcgat
     7801 tccaaaacaa aaaaaataca agaagaattt gcaaagatat ttccttttaa atcaacgttt
     7861 gaaatataat acggtagaaa aatgttattt acctacgcct catatttatg tatattggtg
     7921 ttttctacat cggttgcatt ttgttacgaa cattcaaaaa gtaattttaa agtattgcta
     7981 tggtcagtat tgtttttgtc tctttggata cctgcagcaa ttcgttttgg agtaggcgct
     8041 gattacttcc gatatttaag tatggttgaa tcagttaggt tgggatatat ttctactgaa
     8101 gttggatttt attttataaa ttattttgtt gtttggtttg atttaaatcc acaatggtct
     8161 attgcaatat caagtttcat tgtaatcttt ttaagtttga aggcgattcc acgaaactat
     8221 ttttctgttt cagtttttgt tttagtatgt accttttatc tgccttcgtt ttctcttgtt
     8281 agacaagcta ttgctatagc tttcatagcg tatgcagtga aaaattatat cgatggtaat
     8341 aaaaaaaact atattatttt tattttgata ggctctctat ttcatctctc ggcattatta
     8401 cttgtaccca tttattttat tgcgcgattt agagtccgta gttcaattct ctttttgggt
     8461 attttaataa taagtttatt gctattcctg ggtagtggat taaatgcact tttgaatagt
     8521 gagttttttt tacaaagcaa atacggttat tacgcaacaa gtgatttcat acaggatgct
     8581 aaaatcggaa gtggggtagg tgtatttatc aaaatgctat tacctttaat ttttataaca
     8641 aacgcaaaaa aaatactaaa aacaaatcag aatcttaata ttctattgtg gatgtctgtt
     8701 ggatgtgtgc ttgctaatgt atgttcaatg aagattcata tttttaatcg tctagcagat
     8761 atattccttt ttgttaattt catggtaacc tcatatatta tcaattcatt agaaaaaaat
     8821 gttataaaac aattatcttc aatggcaatt attattgttt ttattgtgtt ttattgcaga
     8881 acaattcagg ccaattataa tgatgattta ggtggcattg gtatttctcc ctacaaaaca
     8941 atactttcta agtgatatta tggatgatta tttagtatca attattatgc cttcatataa
     9001 cgcagagcat acgatttctg catcaattag ttccgtgtta aagcaaacat atgctaactg
     9061 ggaattgctc gtttgcgatg atgattcaag tgataataca agatttaaag ttttggagtt
     9121 ttctgattca agaattaaac ttttaacaaa tgaatacgca aaaggtgcag caggtgctag
     9181 aaataccgct ctaaaatatg caagcggccg atttatagct ttcttagata gtgatgatat
     9241 atggatcgct aacaaattag agatgcaaat atctatgatg ctaaagaata atatttcttt
     9301 tatgtatggt aattatgaaa ttataaataa taattcaata gttggtaagt ttgtagctcc
     9361 ccaaaaaatc acgtacaaca aattgctaaa aaattgtggt attggttgtt tgactgtcgt
     9421 tttagacagg actttactaa atcctttcag ctttcctttc gtgcataaag aagattatta
     9481 tctatggctg agtattctaa aagataacaa tattagtgca attaattgcg gctttatttg
     9541 ttctaaatat agactttcac aaagttcaat ttcatctaat aaatttaaag agttgaaaag
     9601 gcaatgggat gttttggggg attttgttga aaacccattg gctcggatat attatttgct
     9661 aaattatatt gttatcggta taaagaaaca tgcttttgac tataaaaacg gtaaaaaatg
     9721 aaaaaaatag cttttgtaat tgctgatatt acatttgttg gcggtattga gagagtaaat
     9781 acaatacttg ccaataaatt tatcagcgaa ggatactctg tagaaataat atctctctat
     9841 aaaactaatc taaaaattaa ttacgagctt gacaatcaca ttaaaattag ttttgttaat
     9901 gatggctctt atttaggtga gcctggaagt tttggacggc tgaagaaaca catagggtca
     9961 cagtttaaat tatttaaagc cattaaaaag tcaaatgctg atttttatat cattaataca
    10021 ttccctatgg cttttttgtc atttttcagt ctcttttcaa aaaaaaagta tgttgttatt
    10081 gaacatgtac attatgacta ttatagtgca attgttagag cattaagaaa tttattgtat
    10141 agattctttt ataaagtaat ctgtattaat aaaaatgact tgtataagtt ttctaaacat
    10201 ttagataatg ttgttaaaat tagtaacccg ctgagttttt cttgttcaaa agtagctgat
    10261 ttaagcgcaa aaaaaattat ggcggttggc aggctagagc atcaaaaggg ttttgattta
    10321 ttaatagata tttttgcgaa agttaacaaa tcaaatcctg gttgggaact ccatatttat
    10381 ggtgtcggaa cgtgcgaaaa atttctcaca gataaaataa ataagcatgg attaaataat
    10441 gtaaaactga tgggaagtgt cgatcatata caacaatatt atcctaaata ttccattttt
    10501 gcattctcat caaggtttga agggttcggg atggtgttat tggaagcaat ggaatgtggt
    10561 ttgccatgta tttcatttga ttgccctacc ggcccatctg aaatattagg ggatggggag
    10621 tatggaatct tagttgagaa tgggaatctt attaaattta gcgcggaatt agcgaattta
    10681 atgtccgatg aagagaagaa aattaaattt tcaattttat caaagagtcg tgcacaagag
    10741 tttaatatag acaagatttt tgacgaatgg agaaagttaa taagttagta cttatagtaa
    10801 atgactgaaa acgttgtaca aataatttat atcaaaaggt tgcttattcg attaatataa
    10861 ttaaataagt tacctagccc tgacaggagt aaacaatgtc aaagcaacag atcggcgtcg
    10921 tcggtatggc agtgatgggg cgcaaccttg cgctcaacat cgaaagccgt ggttataccg
    10981 tctctatttt caaccgttcc cgtgaaaaga cggaagaagt gattgccgaa aatctaggca
    11041 agaaactggt tccttactat acggtgaaag agtttgttga atctctggaa acgcctcgtc
    11101 gcatcctgtt aatggtgaaa gcaggtgcag gcacggatgc tgctattgat tccctcaagc
    11161 catacctcga taaaggtgac atcatcattg atggtggtaa caccttcttc caggacacca
    11221 ttcgtcgtaa ccgtgagctt tctgcagaag gctttaactt tatcggtacc ggtgtttccg
    11281 gtggtgaaga aggcgcgctg aaaggtcctt ccattatgcc tggtgggcag aaagaagcct
    11341 atgaactggt tgcgccgatc ctgaccaaaa tcgccgcagt ggctgaagac ggtgagccat
    11401 gcgttaccta tattggtgcc gatggtgcag gtcactatgt gaagatggtt cacaacggta
    11461 ttgaatacgg tgatatgcaa ctgattgctg aagcctattc tctgcttaaa ggtggtctga
    11521 acctctccaa cgaagaactg gcgcagacct ttaccgagtg gaataacggt gaactgagca
    11581 gctacctgat tgacatcact aaagacatct tcactaaaaa agatgaagac ggtaactacc
    11641 tggttgatgt gattctggat gaagcagcta acaaaggtac cggtaaatgg accagccaga
    11701 gcgcgctgga tctcggtgaa ccgctgtcgc tgattaccga gtctgtgttt gcacgttata
    11761 tctcttctct gaaagatcag cgtgttgccg catctaaagt tctctctggc ccgcaagcac
    11821 agccagcagg cgacaaggct gagttcatcg aaaaagttcg ccgtgcgctg tatctgggca
    11881 aaatcgtttc ttacgctcag ggcttctctc agctacgcgc cgcgtctgaa gagtacaact
    11941 gggatctgaa ctacggtgaa atcgcgaaga ttttccgtgc tggctgcatc atccgtgcgc
    12001 agttcctgca gaaaatcacc gatgcatatg ccgaaaatcc gcagatcgct aacctgatgc
    12061 tggctccgta cttcaagcaa atcgccgatg actaccagca ggcgctgcgc gatgtcgtcg
    12121 cttacgcggt acagaacggt atcccggttc cgaccttcgc cgctgcggtt gcctattatg
    12181 acagctaccg cgccgctgtt ctgcctgcga acctaatcca ggcacagcgc gacta
//
