LOCUS       EU294167               13011 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Shigella dysenteriae O antigen gene cluster, complete sequence.
ACCESSION   EU294167
VERSION     EU294167.1
KEYWORDS    .
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13011)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 13011)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..13011
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:622"
                     /note="type: 13"
     gene            1137..2222
                     /gene="rmlB"
     CDS             1137..2222
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ACA24782.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSKRYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIHKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     misc_feature    1137..12108
                     /note="O antigen gene cluster"
     gene            2222..3121
                     /gene="rmlD"
     CDS             2222..3121
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ACA24783.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLINATSVEAIAKAANEVGAWAIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLSAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3179..4057
                     /gene="rmlA"
     CDS             3179..4057
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ACA24784.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEKVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            4062..4613
                     /gene="rmlC"
     CDS             4062..4613
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ACA24785.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSCKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFVYKTTNYYAPGYEGSIIWNDKILSIDWPEL
                     TLEYILSDKDLKAPILSESIENI"
     gene            4615..5598
                     /gene="wfgF"
     CDS             4615..5598
                     /gene="wfgF"
                     /note="hydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfgF"
                     /protein_id="ACA24786.1"
                     /translation="MFISRVKRKIQSLISLELNFLHDYWRFKKYYTKGSQASKDKQKL
                     ESWILQDKHRIEKAFSLPKPKLGFGKDVIPRLIDNLVNYSSKFGNDQVYYIGLGALQS
                     YKRFHDEQKFLLPEFYSKNICKIRNDDFLDPRCNIAGYFKKDDSLVTGKITVESLMNE
                     RRSCRHFDVDESLKIGDKLLKDVTKLSITAPSVCNRQHWRIHYFSGELKNKILSYQNG
                     NSGFTENIPYVAVITSDLRAFYSTDERNQPYTDGGIFAMNFMYALQHYGLASCPLNWC
                     NSSHIENEFRETKLIPDYEVVVLVVAFGYPNKDALYAKSPRLSLNNFYTIN"
     gene            5631..7133
                     /gene="wzx"
     CDS             5631..7133
                     /gene="wzx"
                     /note="O antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ACA24787.1"
                     /translation="MKKQIIKNISANFTNLIVSIFIGLLLTPLLVKSLGIEVYGVLPI
                     ALFLTFYMGVIAQALTASVNRFLIEDFIKGAYTEASVVFSTSFILILGYLSVISIGFI
                     YPILHISDFVNIPPGYEHDAVLLFSCVIFSFVIAMISSSLSVSMYAKNRIDLMQYGNI
                     LRNITKVLIIIMFVYLSEINLTKIGISIVLSELFYLIYVIITWKVLTPQLSLQIGFFR
                     KDKVKRLTSFSGWILFDQIGSILFLKTDLILVNSLSGSKANGEYSIATQFSDLLRSLA
                     GLVAGSLGPMMVILYEEKKLEELTSLTITFVKFLSLTIAIPIIILCVYSKEILELWLG
                     SGFVFLSPLICIVTLPLIINLGTLPILSINIAVKKIQVPALINFFLAIVGIIGSVVLF
                     KTTTLGYYAVAISYVITLTIKNSIFIPWYATIILNKPRYLFFKTHLITIIFSSLFFIF
                     IYILKSIMQITNLNLLFELILVGCFGLLSTFPFYSKEERRSFMLMLKRKV"
     gene            7136..8281
                     /gene="wfgG"
     CDS             7136..8281
                     /gene="wfgG"
                     /note="pyruvyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfgG"
                     /protein_id="ACA24788.1"
                     /translation="MDSTQKIGVLNFQYSDHNYGAVLQAAAIEQFLKSNGYNAEHIDY
                     ISSPEIKGNRVIIQLKCILKKLGLSDSVRKMLGKQVHLTPVVTNEKVFEEFRKNWLVR
                     TKCFRNFEELKKTDFNFKAVIVGSDQVWRPSQFTRFSDYKVYFLSFLPSQVKKISYAA
                     SFGIDKWEVSDANVTNEIKKYIQDFNAVSVREDSGVNICATIFNVDAEHVLDPTLLVG
                     SDFFNSIIAKEIRADAYYDAIVYYKLDIDQHFIDSVTILGRKKCKPVKNIYYNQVDGR
                     NEYYSVPEWLSNIKNANFVITDSFHSVCFCLLFQKEFLCCVNESRGLSRLQSLLGMLE
                     LTDRICSSKDDFDNKLNSLKPIDYSKVGDILNKHRDISKAFLLSNLS"
     gene            8306..9196
                     /gene="wfgH"
     CDS             8306..9196
                     /gene="wfgH"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24789.1"
                     /translation="MNANNRNAFPIIVMTRNEGADLQRCVDSILHTVSIDVHIYIIDN
                     NSDDLSHIQILANLEDLNRKNLTVIRNTKNLWILGVNNTLLKIKKNHDTKYFILTDGD
                     IDFHGFKEESCWLTYLINKMESNMIIGKLGISLDWSFLASKTEMKDIYRQEQNLYNER
                     RKIGDLYISAVDTTVAIYRWDWSIERKALFYPDHMRYLRPELYSCRTKRGLTVEHLGW
                     YRYLDNSLSTQSINSKVFCFTIVGGTVKPEVLKLASKPYQYFHILFSKPFAMLWYFRR
                     YFKLFIYFITKGRRGFDGQS"
     gene            9183..10097
                     /gene="wfgI"
     CDS             9183..10097
                     /gene="wfgI"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24790.1"
                     /translation="MDSHNNKLSKRIPSPALRCYAVIVTFNPDINKVRSLIDILQKNF
                     VTAVVVDNTIGAINYPFSCHVINLGDNLGIATAQNKGIEYCLTKQAEAIWFFDQDSVI
                     TSSFVERFLSTVHENPNDKIFAPVFWDEKKGFEYAITNIDSKGNRQKLLSSSFPSDFY
                     SSIVISSGCLIKSDLLMKIGPMLDFLFIDYVDTEWCLRAFYYGYKVHIIKNARMKHSI
                     GDNTISLIGFNVPIHSPLRRYYRIRNAFLLIRFKHIPKKLVFREVIFCCCHQLIIILT
                     QEKKLAYVKYFLRAVYDGLRNKTGKFVN"
     gene            10097..10993
                     /gene="wfgK"
     CDS             10097..10993
                     /gene="wfgK"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24791.1"
                     /translation="MSFVMKPKVLVLVAVYNGEKWLIEQLTSIINQKNVDVDVIINID
                     KSTDSSYKLCENFIADNVKILPYGAVFGGAGANFYHLIHESEFSGYDYIAFSDQDDIW
                     FETKIYDAINRMHDYDAYSSNVIAFWENGKQCLIEKSQPQTEFDYFFEAAGPGCTYIV
                     RQELALSFKYFVCKNYVAVNKVCLHDWLLYAFARERKYKWFIDPQPGMLYRQHSNNQV
                     GANASLLGAIKRIKKIKDSWYRNEVKKIVNLTSIDNNLISECLMRDGYLSSLKLAFIV
                     RKLRRRPRDQFAMFIALVLGWF"
     gene            11011..12108
                     /gene="wzy"
     CDS             11011..12108
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ACA24792.1"
                     /translation="MLILTNNIILLIFIIYTFLVFISALGVKLNSRGLDNFSCLITVL
                     TFVILIFARTGLGVDESTYLESYHYYVQGGEADFEYGFNILFWGIRLFGVTESSFNTI
                     FPLLILTALYFAVTVSVKSPFRSFVLIFTIFSSFFLDFSFNAYRQGLSFWFVLLAIES
                     HLSGNRWKFIVYCLIGLGFHWSAAVVLFMLFLTRFLSTRLAIFCNIFLFFLTLVAAMM
                     PLHVLSMLVTMIQYLPLNSSYLQKVIFYLTTIKSSFYDLNFFGRAPLIIYALILISLL
                     AIYRKLLPIVQFKLLILLLVYSVLLLEMSYSFRNYYWVLPFTPFIMAKILSCYSNKKK
                     VMVMYTMFSTGLWSLSIASFYSFPILSMIFQ"
BASE COUNT     3942 a   2165 c   2696 g   4208 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctcttc ttgaactgcg cgtgaagcgt
      121 cagctgctgg cggaagtgca gtccatctgt ccgccgggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt aggtcactcc attttgtgtg cgcggcctgc cattggtgac
      241 aatccatttg tcgtggtgct gccagacgtt gtgatcgacg acgccagcgc cgacccgctg
      301 cgctacaacc ttgctgccat gattgcacga ttcaacgaaa cgggtcgtag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactcggtca tccagaccaa agaaccactg
      421 gaccgtgaag gtaaagtcag ccgcattgtt gaatttatcg aaaaaccgga tcagccgcag
      481 acgttggact cagacatcat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcactcagcc tggtgcatgg gggcgtattc agctgactga tgccatcgct
      601 gaactggcga aaaaacagtc cgttgatgcc atgctgatga caggtgacag ctacgactgc
      661 ggtaaaaaaa tgggctatat gcaggcgttc gtgaagtatg ggctgcgcaa cctgaaagaa
      721 ggggcgaagt tccgtaaagg gattgagaag ctgttaagcg aataatgaaa atctgaccga
      781 atgtaacggt tgataagaaa attattacgg cagtgaagat tcgtggcgaa agtaatttgt
      841 tgcgaatttt cctgccgttg ttttatataa acaatcagaa taacaacgag ttagcaatag
      901 gattttagtc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtttgaa tttttcgggt ttagcgcgag tgggtaacgc tcgtcacatc gtggacatgt
     1021 atgcagtgct ctggtagctg taaagccagg ggcggtagcg tgcattaata cctctattaa
     1081 tcaaactgag agccgcttat ttcacagcat gctctgaagt aatatggaat aataaagtga
     1141 agatacttgt tactggtggc gcaggattta ttggttctgc tgtagttcgt cacattataa
     1201 ataatacgca ggatagtgtt gttaatgtcg ataaattaac gtacgccgga aacctagaat
     1261 cacttgcaga tgtttctgac tctaaacgct atgtttttga acatgcggat atttgcgatg
     1321 ctgctgcaat ggcgcggatt tttgctcagc atcagccgga tgcagtgatg cacctggctg
     1381 ctgaaagcca tgtggatcgt tcaattacag gccctgcggc atttattgaa accaatattg
     1441 ttggtactta tgtccttttg gaagcggctc gcaattactg gtctgctctt gatggcgaca
     1501 agaaaaatag cttccgtttt catcatattt ctactgacga agtctatggt gatttgcctc
     1561 atcctgacga agtaaataat aaagaacaat tacccctctt tactgagacg acagcttacg
     1621 cgcctagtag tccttattcc gcatcaaaag catccagcga tcatttagtc cgcgcgtgga
     1681 aacgtaccta tggtttaccg accattgtga ctaactgttc gaataactac ggtccttatc
     1741 actttccgga aaaattgatt ccactagtaa ttcttaatgc tctggaaggt aaggcattac
     1801 ctatttatgg caaaggggat caaattcgtg actggctgta tgttgaagat catgcgcgtg
     1861 cgttatatac cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacaca
     1921 acgaaaagaa aaacatcgat gtagtgctca ctatttgtga tttgttggat gagattgtac
     1981 cgaaagagaa atcttaccgc gagcaaatta cctatgttgc cgatcgcccg ggacacgatc
     2041 gccgttatgc gattgatgca gagaagatta gccgcgaatt gggctggaaa ccgcaggaaa
     2101 cgtttgagag cgggattcat aaaacggtgg aatggtacct gtccaataca aaatgggttg
     2161 ataatgtgaa aagtggtgcc tatcaatcgt ggattgaaca gaattatgag ggccgccagt
     2221 aatgaatatc ctccttttcg gcaaaacagg gcaggtaggt tgggaactac agcgtgctct
     2281 ggcacctctg ggtaatttga ttgctcttga tgttcactcc actgattact gtggtgattt
     2341 tagtaatcct gagggtgtag ctgaaaccgt aagaagcatt cggcctgata ttattgtcaa
     2401 cgcagccgct cacaccgcag tagacaaagc agaatcagaa ccggagtttg cacaattaat
     2461 taacgcgacg agtgtcgaag cgatcgcgaa agcagccaat gaagtcggcg cctgggctat
     2521 tcactactct actgactacg tatttccggg aacaggtgaa ataccatggc aggaggcgga
     2581 tgcaaccgca ccgctgaacg tttatggaga aaccaagtta gccggagaaa aagcattaca
     2641 agagcattgt gcgaagcacc ttattttccg gaccagctgg gtctatgcag gtaaaggaaa
     2701 taacttcgcc aaaacgatgt tgcgtctggc aaaagagcgc gaagaactag ccgttattaa
     2761 tgatcagttt ggtgcgccaa cgggcgcaga gttgctggct gattgtacgg cacatgcaat
     2821 tcgtgtggca ctgaataaac cagaagtcgc aggtttgtat catctggtag ccagtggtac
     2881 cacaacttgg cacgattatg ctgcgctggt ttttgaagag gcgcgcaaag caggcattcc
     2941 ccttgcactc aacaagctca gtgcagtacc aacaacagcc tatcctacac cagcccgtcg
     3001 cccccataac tctcgtctta atacagaaaa atttcagcag aactttgcgc ttgtcttgcc
     3061 tgactggcag gttggtgtga aacgaatgct caacgaatta tttacgacta cagcaattta
     3121 atagtttttg catcttgttc gtgatggtgg agcaagatga attaaaagga atgatgaaat
     3181 gaaaacgcgt aaaggtatta ttttagcggg tggttctggt acacgtcttt atcctgtgac
     3241 tatggctgtc agtaaacagc tattacctat ttatgataag ccgatgatct attacccgct
     3301 ttctacactg atgttagcgg gtattcgcga tattctgatt atcagtacgc cacaggatac
     3361 tcctcgtttt caacaactgc tgggtgacgg tagccagtgg gggctaaatc ttcagtacaa
     3421 agtgcaaccg agtccggatg gtcttgcgca ggcgtttatt atcggtgaag agtttattgg
     3481 tggtgatgat tgtgctttgg tacttggtga taatatcttc tacggtcacg acctgcctaa
     3541 gttaatggat gccgctgtta acaaagaaag tggtgcaacg gtatttgcct atcacgttaa
     3601 tgatcctgaa cgctatggtg tcgttgagtt tgataaaaac ggtacggcga tcagcctgga
     3661 agaaaaaccg ctacaaccaa aaagtaatta tgcggtaacc gggctttatt tttatgataa
     3721 cgacgttgtc gaaatggcga aaaatcttaa gccttctgcc cgcggtgaac tggaaattac
     3781 cgatattaac cgtatctata tggaacaagg gcgtttatct gttgccatga tggggcgtgg
     3841 ttatgcgtgg ttagacacgg ggacacatca gagcctgatt gaggcaagca actttattgc
     3901 aacaattgaa gagcgtcagg ggttgaaagt ttcctgcccg gaagaaattg cttaccgtaa
     3961 agggtttatt gatgctgaga aggtgaaagt attagctgaa ccgctgaaaa aaaatgctta
     4021 tggtcagtat ctgctgaaaa tgattaaagg ttattaataa aatgaatgta attaaaacag
     4081 aaattccaga tgtactgatt tttgaaccga aagtttttgg tgatgagcgt ggtttctttt
     4141 ttgagagctt taaccaaaag gtttttgagg aagctgtagg ccgcaaagtt gaatttgtcc
     4201 aggataacca ttcgaagtct tgtaaaggtg ttttacgcgg gctgcattat cagttagaac
     4261 cttatgcgca agggaaattg gtacgttgcg ttgttggtga ggtttttgat gttgctgttg
     4321 atattcgtaa atcgtcacca acttttggaa aatgggttgg ggtgaattta tctgctgaga
     4381 ataagcgcca gttgtggatc cctgaaggat ttgcacatgg ttttttggtg ctaagtgaga
     4441 cggcagaatt tgtttataag acgacgaatt attatgctcc tgggtatgaa ggtagcatca
     4501 tttggaatga taaaatatta tctattgatt ggcctgagtt aacactggag tatattcttt
     4561 ccgataaaga tctaaaggcc ccaattctaa gtgaatctat tgagaatatt taaaatgttt
     4621 attagtagag taaaacgtaa aattcaatct ttgataagcc ttgagttgaa ttttttacac
     4681 gattattgga ggtttaaaaa atattacacg aagggatctc aggcttcgaa agataagcag
     4741 aagcttgaat cttggatatt gcaagataaa catcgaatag aaaaagcttt ctctttacct
     4801 aagccaaaac ttggatttgg taaagatgta atacctcgtc tcattgacaa tcttgttaat
     4861 tatagcagta aatttggaaa tgatcaggta tattatattg gcttaggtgc attacaatcg
     4921 tataagagat tccacgacga acaaaaattt ttattgcctg aattttattc aaagaatatt
     4981 tgtaaaatac ggaatgatga ttttctcgat ccccgatgca atatcgctgg ctattttaaa
     5041 aaagatgatt cattagttac aggtaagata acagtagaat cattgatgaa cgaacggcgt
     5101 agctgtcggc attttgatgt tgatgaaagt cttaaaatcg gagataaact tttaaaggat
     5161 gttacaaaac tatctattac agccccttct gtttgtaata gacaacattg gcgcattcac
     5221 tatttttcag gtgaattaaa gaataaaatt cttagttatc aaaatggaaa ttcaggattt
     5281 acagaaaaca taccttatgt agcagttata acaagtgatc ttagagcatt ttattctact
     5341 gacgaaagaa accagcccta tacagacggt gggatctttg ccatgaattt tatgtatgct
     5401 ctacaacact atggtcttgc ttcttgtcca ttgaattggt gtaattcatc tcacattgag
     5461 aatgagttta gagaaactaa gttgattcct gattacgaag tagtagtact tgtggttgct
     5521 tttggctatc caaataaaga tgccttatat gctaaatcac cacgattgtc tctaaataac
     5581 ttttacacaa ttaattaggc gaaggccacc tttaaaggtg gctgacaata atgaagaaac
     5641 aaataatcaa aaatatttca gccaatttta ctaatctgat tgttagtatt tttattggac
     5701 tattactgac gcctttactc gtcaaaagtt taggcattga agtatatgga gttctaccga
     5761 tagctttatt tttaacattt tatatgggag tcattgctca agcattaact gcatctgtaa
     5821 atagattttt aattgaggat tttattaaag gagcatatac tgaggcaagt gtcgtattta
     5881 gtacatcgtt tattcttatt ctaggatatc tatctgttat tagtattgga tttatttatc
     5941 caatattgca tataagtgat tttgttaaca taccacctgg atatgaacat gatgcagttc
     6001 ttctgttctc gtgtgtaata ttttcgtttg ttattgctat gatcagttca tcattgtcag
     6061 tttctatgta tgcaaaaaat aggattgact tgatgcaata tgggaacatt ttaaggaata
     6121 ttactaaagt cttaatcatt ataatgtttg tttatttatc tgaaataaat ttaacaaaaa
     6181 taggaatatc aattgtttta tctgagctgt tttatctaat ctatgtcatt ataacttgga
     6241 aagtattgac accacaactt tctctgcaga taggattttt cagaaaggat aaggtaaaga
     6301 gattaacatc tttcagtggc tggatattgt ttgatcaaat tgggtcaatt ctttttctta
     6361 aaacagatct gattttagtt aattctctaa gtggtagtaa agctaatggt gaatattcaa
     6421 tagctacaca atttagtgat cttttgcgtt cacttgctgg tttggtagct ggtagtttgg
     6481 gtcctatgat ggtaatttta tatgaagaaa agaaattaga agaacttact tcattgacca
     6541 ttacatttgt taaattccta agcttgacga tagctattcc tattattatt ctttgtgtat
     6601 actctaaaga aattcttgag ctttggttag gttcggggtt tgtatttctt tcaccattaa
     6661 tatgtatcgt aacgcttcca ctgattatca atttgggcac actgccaata ttatcgatca
     6721 atattgcagt caaaaaaatt caagtgcctg cactcataaa tttctttctt gcaatcgttg
     6781 gaattatagg ctctgtagta ttgttcaaaa ccactacatt aggttattat gctgtagcta
     6841 taagttatgt gattacatta actattaaga actccatttt cataccatgg tatgcaacaa
     6901 ttatattaaa taaaccaagg tacctttttt ttaaaactca tctaattact atcatcttct
     6961 cttctctatt ttttattttt atatatatac ttaaaagtat aatgcagatt actaatttaa
     7021 atttgctttt tgaattgata ttggttggtt gctttggact tctatctaca tttcctttct
     7081 atagtaaaga ggaaaggcga tcatttatgt taatgctcaa gaggaaagtg taataatgga
     7141 ttcgactcag aaaataggcg ttctgaactt ccagtattcg gatcataatt atggtgctgt
     7201 tttacaagcc gcagcaatag aacaatttct gaaaagcaat ggatataatg ctgaacatat
     7261 agactatata tctagtccag agataaaggg taatagggta attatacaac taaaatgtat
     7321 attgaaaaaa ttgggactat ctgattctgt aagaaaaatg cttggaaaac aagtccactt
     7381 gacacctgtg gtcacaaacg aaaaagtatt tgaggaattt agaaaaaatt ggttggtgag
     7441 aacgaagtgt ttcagaaact ttgaagaatt aaaaaaaact gattttaatt ttaaagcggt
     7501 tatagttggg agtgatcagg tttggcgacc atctcaattt actcggtttt ccgattataa
     7561 ggtatatttc ttaagctttt taccttcgca ggttaagaaa atatcttatg cagcaagttt
     7621 tggtattgat aagtgggaag ttagtgatgc taacgtcacg aacgaaataa aaaaatatat
     7681 tcaagatttc aatgctgttt cagtcagaga agatagtggc gttaatattt gtgcaaccat
     7741 ttttaatgtc gatgctgaac atgttcttga tcctacttta ctggttggta gtgacttttt
     7801 taacagtata atagctaagg aaatacgtgc tgatgcttat tacgatgcta tagtttatta
     7861 caaacttgat attgatcaac attttataga ttcagttact atactaggta gaaagaaatg
     7921 caagccagta aaaaatattt attacaacca agtagatggc cgaaatgagt attattctgt
     7981 tcctgaatgg ttaagcaata ttaagaatgc taattttgtt atcacagatt cttttcatag
     8041 tgtatgtttt tgccttcttt ttcaaaaaga gtttttatgc tgtgtgaatg aaagtagagg
     8101 attatctaga ttgcaaagtt tacttggaat gcttgagctt actgatcgta tttgctcgtc
     8161 aaaagatgat tttgataata agttaaattc gttaaagcca atagactatt ctaaagtggg
     8221 cgatatattg aataagcata gagatatttc taaggcgttt ttattaagta atttatcttg
     8281 agtttgagtg tgggttaaat atttaatgaa tgcaaataat agaaatgcat ttccgatcat
     8341 tgttatgact cgcaacgaag gcgccgactt acaacgttgc gttgattcta tattacatac
     8401 tgtatctata gatgtacata tatatatcat agacaataat tctgatgatt tatcacatat
     8461 tcagatcctt gctaaccttg aagacctgaa tcgtaaaaat ttaacagtta taagaaatac
     8521 aaagaattta tggattttag gtgtaaataa cacactattg aaaatcaaga aaaatcatga
     8581 tactaaatat tttattctca ctgatggtga tatagacttt catggtttta aagaagaaag
     8641 ttgttggtta acatatttaa tcaataaaat ggaatccaac atgataatcg gtaagctcgg
     8701 aatttctctg gattggtcat ttctggcctc aaaaacggag atgaaagata tttaccggca
     8761 agaacaaaat ctctataatg aaagaagaaa aatcggtgat ctttatatat ctgccgttga
     8821 cacgacagta gccatttatc gttgggattg gtctatagag cgtaaagctt tattttatcc
     8881 tgaccatatg aggtatttac gtccagaact gtactcttgc cgtactaaac gtggtttaac
     8941 agtagagcat ttaggatggt atagatacct tgataactcc ctgtctacgc aaagtataaa
     9001 ctcgaaagtt ttttgtttta caattgttgg tggtactgtt aaacctgagg ttttaaaact
     9061 ggctagtaaa ccctatcagt atttccatat tttgttttca aaacctttcg ctatgctttg
     9121 gtatttcaga cgatatttta aactttttat atattttatt actaaaggga ggcgtggatt
     9181 tgatggacag tcataataat aagttatcaa aacgtattcc gtcacctgca ctacgttgtt
     9241 atgcagtaat agttacattt aaccctgata ttaataaggt tagaagttta atagatattc
     9301 tacaaaagaa ttttgtcacc gcagttgtag tagataatac gattggagca ataaattatc
     9361 cattctcatg tcatgttatt aatttaggtg ataatttagg tattgccaca gcacaaaata
     9421 aaggcataga atattgtttg actaaacaag ctgaagctat ctggtttttt gatcaggaca
     9481 gcgtaatcac ttcttcattt gtcgaaagat tcctatctac tgtccatgaa aatcctaatg
     9541 ataagatctt tgctcctgtg ttctgggatg agaagaaagg gtttgaatat gcaattacaa
     9601 atatagattc aaagggcaac agacaaaagt tgttatcatc atcttttcca tcggattttt
     9661 atagttcgat tgttatttca tcagggtgct taataaaaag tgatttgctg atgaaaattg
     9721 gtccaatgct agatttttta tttatagatt acgttgatac ggaatggtgt ttacgtgctt
     9781 tctattatgg ctacaaagtt cacataatca aaaatgcaag gatgaaacat tctataggtg
     9841 ataacactat ttctttaatc ggttttaatg taccaattca ctctcctctt cgacgttatt
     9901 atcgaataag aaatgcattt ttattgatta ggtttaagca tatcccaaaa aaactagtat
     9961 ttagagaagt gatattttgt tgctgccacc aattaataat tatcctgacc caagaaaaaa
    10021 aactggcata tgttaagtat tttttacggg ctgtatatga tggccttaga aataaaactg
    10081 ggaagtttgt aaactaatga gttttgtcat gaagccaaag gtattagtgc tagttgcagt
    10141 ttataatggt gaaaagtggt taatagagca acttacatct ataataaatc aaaaaaatgt
    10201 tgatgttgat gtgattatta atatcgataa atcaactgat tcttcataca aattgtgtga
    10261 gaattttatt gcagataatg tcaagatact accgtacgga gctgtatttg gcggtgcagg
    10321 tgccaatttt tatcatctaa tccatgaatc tgaattttct ggttacgatt acattgcatt
    10381 ttccgatcaa gacgatattt ggtttgaaac taaaatatat gatgccatca ataggatgca
    10441 tgattatgat gcatattcaa gtaatgtaat agcattctgg gaaaatggca aacaatgtct
    10501 aattgagaaa tctcaaccac aaacagaatt tgattatttt tttgaggcag caggacccgg
    10561 gtgcacttat atagtgagac aggaactagc actctcattt aaatatttcg tttgcaagaa
    10621 ttatgtggct gtaaataaag tttgtttaca tgattggctg ttatatgcgt ttgccagaga
    10681 aagaaaatat aaatggttta ttgatcctca acctggtatg ttgtatagac agcattcgaa
    10741 taatcaagtt ggggcaaatg cttcattatt aggtgctatc aagcgtataa aaaaaattaa
    10801 agattcttgg tatagaaatg aggtaaaaaa aatcgtcaat ttaacttcga ttgataataa
    10861 tttaataagt gaatgcctta tgagagatgg atacttatca tctttaaaat tggcttttat
    10921 agttagaaaa ttaagacgtc gtcctcgaga tcaatttgct atgtttatag ccttagttct
    10981 gggctggttt taacatcgat cgggagcact atgctcattt taacaaacaa tattatattg
    11041 ctgattttta taatctatac ttttttggta tttataagtg cacttggagt aaaattaaat
    11101 agcagagggc ttgataattt ttcgtgcctc attacagtcc tgacatttgt tatactcatt
    11161 ttcgctcgta caggattagg tgttgacgaa agtacatatt tagaatctta tcattattat
    11221 gttcaaggag gtgaagcaga ttttgaatac ggatttaata tccttttttg gggaattaga
    11281 ctattcgggg taacagaatc ttcattcaat accatattcc cgttattgat attaacagca
    11341 ttatacttcg ctgttactgt atcagttaaa agtcctttta gatcttttgt attaatattt
    11401 acaatatttt cttctttctt tctggatttt tcatttaatg catataggca ggggctttca
    11461 ttctggtttg ttctacttgc catagaatca catctgtctg ggaataggtg gaaatttata
    11521 gtttattgct taattggact tggtttccac tggtcagcag cagttgtttt attcatgctt
    11581 ttcctaacaa gatttttatc tacaaggtta gcgatattct gtaatatttt tctatttttc
    11641 ttaacgctag tggcagcaat gatgccatta catgtacttt caatgcttgt aacgatgata
    11701 caatacttgc cattaaactc atcttacttg caaaaagtta ttttttatct tactacaatt
    11761 aagtcttcgt tttacgattt aaatttcttt ggacgtgctc cattaataat ttatgcgctt
    11821 attcttattt ctctcttggc tatatataga aaactattac caattgtgca atttaaattg
    11881 ttaattcttt tattagtcta cagtgtgttg ttacttgaga tgtcatacag ttttagaaat
    11941 tactactggg tgctaccatt tactccattt attatggcga aaattttgag ttgttactct
    12001 aataagaaaa aggtaatggt aatgtatact atgttttcta cagggttgtg gtctctatca
    12061 atagctagtt tctattcatt tcctatacta tctatgatat ttcagtgaaa tcatataata
    12121 atatggttca ttataaatac ttttttggtt aataatattt tatataaact gtgcattaac
    12181 gcacggtgac cacccatgac aggagtaaac aatgtcaaag caacagatcg gcgtcgtcgg
    12241 tatggcagtg atggggcgca accttgcgct caacatcgaa agccgtggtt ataccgtctc
    12301 tattttcaac cgttcccgtg aaaagacgga agaagtgatt gccgaaaatc caggcaagaa
    12361 actggttcct tactatacgg tgaaagagtt tgttgaatct cctgaaacgc ctcgtcgcat
    12421 cctgttaatg gtgaaagcag gtgcaggcac ggatgctgct attgattctc tcaagccata
    12481 tctctataaa ggtgacatca tcattgatgg tggtaacacc ttcttccagg acaccattcg
    12541 tcgtaaccgt gagctgtctg cagaaggttt taactttatc ggtaccggtg tttccggtgg
    12601 tgaagaaggt gcgctgaaag gaccttccat catgcctggt ggccagaaag aagcctatga
    12661 actggttgca ccgatcctga ccaaaatcgc cgcagtggct gaagatgggg agccatgtgt
    12721 tacctatatt ggtgccgatg gcgcaggtca ctatgtaaag atggttcaca acggtattga
    12781 atacggcgat atgcagctga ttgctgaagc ctattctctg cttaaaggtg gtctgaacct
    12841 caccaacgaa gaacttgcgc agacctttac cgagtggaat aacggtgaac taagtagcta
    12901 cctgatcgac attaccaaag acatcttcac caaaaaagat gaagacggta actacctggg
    12961 ttgatgtgat cctggatgaa gcggctaaca aaggtaccgg taaatggacc a
//
