LOCUS       EU296415               12629 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Shigella dysenteriae type 3 O-antigen gene cluster, complete
            sequence.
ACCESSION   EU296415
VERSION     EU296415.1
KEYWORDS    .
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12629)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 12629)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..12629
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:622"
                     /note="type: 3"
     gene            1111..2625
                     /gene="wzx"
     CDS             1111..2625
                     /gene="wzx"
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ACD37100.1"
                     /translation="MSRNKIIIKNTMFLATRTLVSLSVSFYTTRIVIQQLGAADYGLF
                     NIIYGVVTFFTFVVTAMNDSVQRYIAIGVGSQKISVIRDAVKNSMFIFVISAFILAVC
                     LLLARGVIIHNILNIPKDSIENASVLYLVAVFSITILIIQTPLNAMVLAYEKMSFYAY
                     MMIFEMVAKMSMALLLTLLEKDKVIVYSILLGSISFFNLLVYLCYCLICFKKSMFGGR
                     IKFRVLKEISTFSFWNIFGNFSYMCRVQGVNIVINIFYAIAVNAAYAISITVLNAINT
                     LTQSLITALRPQIFKSYGECDLKRYNHLVLFGSKYTFSILFLLSSPVILCADELLKIW
                     LDIVPDYTVEFVRLVIVVAFIDSFSYSMIAGIQATGRIKTYQLVVSLIVLINLPLTFI
                     LFKAGNNVLSMFYPFIVTAIINQGLRLYFIYINAGFDYKKYFTVVIYPCLLAVCLSLV
                     TDISIKKMLPFNSIIDVLIVCIFIFSFNTIIFYWVVVSKKEKKWLLETLKRKVK"
     misc_feature    1111..11208
                     /note="O-antigen gene cluster"
     gene            2589..3971
                     /gene="wfeO"
     CDS             2589..3971
                     /gene="wfeO"
                     /note="hydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeO"
                     /protein_id="ACD37101.1"
                     /translation="MVIRNPEKKSEITIKDVIDNELCTGCGVCISEDSSKTSFMKWNS
                     EGFYEPCFSPVSTLFNMQRVCPFNLSRDTLVNEDELAHEFFDGKGYLDSEVGFYKKIY
                     VGYSKHFRETSSSGGIATYVFEQLLRRKYVDALFIVRELGGSYGYQVFDNPEHIKDMS
                     KTRYYPVTLEKLFDNIYKLNGRVAVSGVACFIKAIRLKQHYHPELKEKIPFLVGIICG
                     GLKSRYYTDYLSQSAGCVSEYQNAEYRVKKKDSHALDYRFTCVEKSNNIIHSVDMQRM
                     GDMWGSGLFKANACDYCDDVTTELADISLGDAWISPYNMDGAGNNVVVCRSTTAHEII
                     LSGIEKKDLELTELELEQLKLSQQGSFNHRHKGLLYRIKNAEKNNRLVPVKRKRFLRS
                     ISFLLKLIQKQRSVTRRKSIEIWMETQNSATFDKKMKGYLFTLRWLTVVNRKLSRMFK
                     IVSLNRMGKK"
     gene            3968..5104
                     /gene="wfeP"
     CDS             3968..5104
                     /gene="wfeP"
                     /note="pyruvyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeP"
                     /protein_id="ACD37102.1"
                     /translation="MKVAILTQPLHTNYGGTLQAYALQKVLINLGHEPETINYRSKIK
                     RPLFIRVVLSKIKRIVLCRKITFDFTTQDRINIRTHHQSFIDTRLNYSEEINGTEGLR
                     DYILKNNYGAVIIGSDQTWRPIYSPRIDSFFLDFLSDVNDIKKMAYAASFGTDKWEFT
                     ESQTNLFKLLLSKFDYVSVRESSGVKLCSEKFGLKAELVLDPTLLLTFEDYTHLLDND
                     YINLHKGKVFSYVLDENYDKKNSIDTVASTLRTTSFYTYPKKITKDEYVIRNYSEYEY
                     PPIEYWISSFKAAEFIVTDSFHGTVFSIIFNKPFIAIANEERGKARFTSLLEMFGLER
                     RLVSNLDDVDLKLVNEKINYGPINEKIAYFREECLKKLQLMLIK"
     gene            5151..6155
                     /gene="wfeQ"
     CDS             5151..6155
                     /gene="wfeQ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37103.1"
                     /translation="MKNCKVSVIIPVYNAEKYIQRCILSLLKQTLDDVEIIIIDDGST
                     DNSLSIIKETVALHTASRARQKRINIISRENKGVAYTRSQGLRLSQGEFIIHFDSDDW
                     AKSNMLEEMYKTIVSNNADMVICDYFLVKNNKEILIKQRVEYEPKKCIRYLLTGELEG
                     FTWNKLIRKKYIDKNKIDFVNKITYMEDFLFILSVLLHNPKIIFQDCAYYYYQKSNPK
                     SLTSHASVDRLSEMIKAVSEIEKKINKYNLQKYLNNEFQLFKLKQKIWFISISKLNVN
                     DNVWNLFPETNPFISKVNVLFYYKIVLFLDSIKLRFFSNKIIYLIGLVQALLQERKEK
                     "
     gene            6152..6940
                     /gene="wfeR"
     CDS             6152..6940
                     /gene="wfeR"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37104.1"
                     /translation="MIPKIIHYCWFGRSPLSELTKQCIASWEKYCPEYKIIRWDENNV
                     DLNSCSFVRQAYKEKKWAFVSDYVRLKVVNEYGGIYLDTDVELIKPLDDLLIYPAYIG
                     FEINKEWYVNSGLGFGSVNNNPVLESLIMEYENINFVNEDGTLNITPCPIRETKALTK
                     IGLIPDGQCQSFDNIVIFSADYFCPVSITGERNFSDKTYSIHHYDASWFSEQKRKGLQ
                     RKKRFIKLFGNLIGTYINKPFIFVDECREFGLVKAIKNMRSNFP"
     gene            7014..8117
                     /gene="wzy"
     CDS             7014..8117
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ACD37105.1"
                     /translation="MYFLLFFSIFYFVYVILIGTLCHNYKALLIFSLIPLTIVSGIRY
                     NVGFDFMSYVDYFNQLKYSNDIYLDSTFKYISYFTYYIGGNEQILFLIYAVFYSVALY
                     FLIKLVLENYITEINNFYTIGLVLSFYSFYFLLSFNQIRAVLSALILCYGLLKNKIDF
                     SFIITITLSILFHSAAMFILPLYFVLRKINVTVLLVIFPFLVVASSFNIFSDIVRFIL
                     TLLNSRFLTYFNSEYFVPRTGMEKMYSMISMIIVLGMVVCLSKLLPKKFDLMIKFVIL
                     FVLLRAMSIDILIFARFSDFLKPMAIILIFTAIYFSSNKVRPRIILPFYLIMTLFICI
                     FNIIIGSNITKENYYTYGYNICFFGNKCIDKFY"
     gene            8146..9195
                     /gene="wfeS"
     CDS             8146..9195
                     /gene="wfeS"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37106.1"
                     /translation="MEKYMNNFSCKEPPSFIENKIILSIILPVYNVSEYLIECLNSLL
                     LDVHERYLNRCEVIVVDDGSTDNSFELMREYSLKYPESIKIYSKFNGGLSDARNYGLL
                     KSSGKYISFVDSDDVVNRGFIKEIINFIDAYDFDILSFDFMKFFNNNGVLILSQIDEF
                     SKMAERVDSEFYKSKPVFAWNKVYRRSLFDNEVFPKGWYYEDVALIPLLLDRAKVLYH
                     INSVCYFYRQRQGAITFFYDNKYLDILKGVSFLYDRSQSSFIKTIIINQFFTLTLLSL
                     RLPTDNYFMNMRGIIDTYCEKFDLDSFEPKLILKHIPFLLLKKLKSLCVYPLYLFKPA
                     VFLHKQIKHFRGKLK"
     gene            9192..10301
                     /gene="glf"
     CDS             9192..10301
                     /gene="glf"
                     /note="UDP-galactopyranose mutase"
                     /codon_start=1
                     /transl_table=11
                     /product="Glf"
                     /protein_id="ACD37107.1"
                     /translation="MKYDYIIIGSGLFGSVFAHELGKMKKKVKVIERRDHVGGNVYCE
                     NIEGINVHKYGAHIFHTNDKEIWDYVNQFVEFNRYTNSPLAFSKGKIYNLPFNMNTFN
                     QLWGAITPQHAKEIIKKQSGEISGNKPRNLEEQAISLVGRDIYNCLIKEYTEKQWGRP
                     CTELPSFIIKRLPVRFTYDNNYFNDQYQGIPVGGYNKLIDALLNGIEVQTCTDYFEEK
                     KYWDSIAKKIIYTGPIDKFYCYKFGALDYRSLKFEHELLNVDNFQGNAVVNYIDKKFP
                     FTRIIEHKFFEFGEQHNTLITREYPKEFEEGDEYYYPVNDEKNMQIYKMYYNLSKQES
                     NVIFGGRLAEYKYYDMHQVIKSALNAFRKEVRLGE"
     gene            10294..11208
                     /gene="wfeT"
     CDS             10294..11208
                     /gene="wfeT"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37108.1"
                     /translation="MNKEVIAVIVTYNRKALLLKVIDAVINQSYPLKKILIIDNNSTD
                     GTEMFISNRLSDVVKYKNTGDNLGGAGGFYRGFIEAEKYGYDYLWLMDDDCMPTVNCL
                     EILISNNPDGIVQPVRYNLDETCAELSPLTYDLSNPFKLNPKGTPLKNYLNTIGNKSA
                     KVDIEAIPFEGPLISRKVVEKIGYPEPKFFIFCDDIEYAIKAKRKGIPIQCNLKAKAY
                     RLLVNNQGNDLLSWKGYFMLRNLFYLHKTYGTNFLVRQKPIVLGLGYALSCVLKCNFS
                     QLPVIWRAFWDSSTLRNTEKFRPEMRSK"
BASE COUNT     4091 a   1859 c   2437 g   4242 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgagcagcg cgtgaagcgt
      121 caactgttgg cggaagtaca atctatctgt ccgccgggcg tgaccattat gaacgtgcgt
      181 cagggtgaac ctttaggttt aggccactcc atcttatgtg cgcgacctgc cattggtgac
      241 aacccattta tcgtggtact gccagacgta gtgatcgacg acgccagcgc cgacccgctg
      301 cgctacaacc ttgctgccat gattgcgcgc ttcaacgaaa cgggccgtag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactccgtca tccagaccaa agaaccgctg
      421 gaccgtgaag gtaaagtcag ccgcattgtt gaatttatcg aaaaaccgga tcagccgcag
      481 acgctggact cagacatcat ggccgtaggt cgctatgtgc tttctgccga tatttggccg
      541 gaactagaac gcactcagcc tggtgcatgg gggcgtattc agctgactga tgctattgcc
      601 gaactggcga aaaaacagtc cgttgatgcc atgctgatga ctggagacag ctacgactgc
      661 ggtaaaaaaa tgggctatat gcaggcgttt gtgaagtacg gactgcgtaa cctgaaagaa
      721 ggggcgaagt tccgcaaagg tattgagagg ttgttgaatg attaaaaaca tgaacaaata
      781 taacggtaaa taagagaaat tgtaacggca gtgaggattt gcggcgaagc tcaaacgtgg
      841 cgaatatccc tgtcgttatt tattaataaa tcatcgtact aacaatggaa taaaaaagtg
      901 ccttgtttta agttttacag gatattcctt gtttctggag gagattgata aggcaatcag
      961 catctgaatt tatcagaact tgtgtgtctt gctgattaca ttgttatatt catgcagtgt
     1021 actggtagct gtggagccag gggcggtagc gtgcattaat attcacaata tattctttta
     1081 aatgtaagca gaattaatcg aacctaaata atgtcacgta acaaaataat cataaagaat
     1141 actatgtttt tggcaacaag aactttagtt tctttaagtg tttcatttta cacaacaaga
     1201 atagttatac aacagttggg ggcggcagac tatggcttat ttaatatcat ttatggagtt
     1261 gtaacatttt ttacattcgt agtcaccgcg atgaatgatt ctgtacagcg ttatattgca
     1321 ataggtgttg gttcacaaaa aatatcggtt attagagatg ctgtaaaaaa tagtatgttt
     1381 atatttgtta tatctgcttt catattggca gtatgtcttc ttttggcaag gggggtgatc
     1441 atacataata ttttaaatat acctaaagat tctattgaaa atgcaagtgt gttatatttg
     1501 gttgcagtat tttcaataac catacttata attcagacgc cacttaatgc gatggtgtta
     1561 gcatacgaga aaatgtcatt ttacgcatac atgatgatat ttgaaatggt agctaaaatg
     1621 agtatggctt tgttactaac tttactggaa aaagataagg tcattgtata ttcaatactt
     1681 ttaggttcta tttctttttt taacttgttg gtttatttat gctactgctt gatatgtttt
     1741 aaaaaatcaa tgtttggtgg gagaataaaa tttagagtgc tgaaagaaat ttcaactttt
     1801 tcattttgga atatatttgg taatttctct tacatgtgca gagttcaggg agtgaacatt
     1861 gtaattaata ttttttacgc tatagccgtg aatgcagcat atgcgatttc gattactgtt
     1921 ttaaatgcaa ttaatacact tacgcaatca ttaattacag cattaaggcc tcaaatattt
     1981 aaatcatatg gcgaatgtga tttaaaaaga tataatcatc tagttctctt tggttcaaaa
     2041 tatacatttt caatattgtt tctgttaagt agccctgtaa ttctatgtgc ggatgagttg
     2101 ttaaagatat ggcttgatat tgtaccagat tatacagttg aatttgtgag gttggtcatt
     2161 gttgtggctt ttattgatag tttttcatat agcatgattg ctggtattca ggctacgggt
     2221 aggattaaaa catatcaact agttgttagt ttaattgtcc taattaattt gccgttaacc
     2281 tttattttgt ttaaggcagg aaataatgtt ttaagtatgt tttatccatt tattgttact
     2341 gccattatca atcaaggttt aaggttgtat tttatctata tcaatgcagg ttttgattat
     2401 aagaagtatt tcactgttgt gatctatcct tgcttgttag ctgtatgctt atcgttagtg
     2461 acagatattt caattaaaaa aatgcttcca tttaacagta ttattgatgt tttaattgtg
     2521 tgtattttta ttttttcatt caatacaata attttctatt gggttgtagt ttctaaaaag
     2581 gaaaaaaaat ggttattaga aaccctgaaa agaaaagtga aataacaata aaagatgtta
     2641 tagacaatga actctgtact ggttgtggag tttgtatttc ggaagatagt agcaaaacct
     2701 cctttatgaa atggaattct gaaggttttt atgagccatg tttctctcct gtatcaacat
     2761 tattcaatat gcagcgtgtt tgtcccttta acttaagtag ggatacatta gtgaatgagg
     2821 atgaacttgc gcatgagttt tttgatggta agggatattt agattctgag gttgggtttt
     2881 ataaaaaaat atatgtgggt tattctaaac attttagaga gacttcatcc tccggtggta
     2941 tagctacata tgtatttgaa caacttctta gacgtaagta tgttgatgct ctgtttatag
     3001 ttagggagct tggtggttca tatggatatc aagtcttcga taatccagaa catattaagg
     3061 atatgtctaa aacaagatat tatcctgtaa cgctagaaaa gttatttgat aatatttata
     3121 agcttaatgg tagagtggcc gtttcaggag tggcatgttt tattaaagcc attaggctca
     3181 aacaacatta ccatccagaa ctgaaagaga aaataccatt ccttgttggt ataatttgtg
     3241 gaggactcaa aagtcgatat tacactgatt atttatcaca gtctgctggt tgtgtcagtg
     3301 agtaccaaaa tgctgagtat cgagtgaaaa agaaagatag tcatgctctt gactatcgtt
     3361 tcacttgtgt tgagaaatca aataatataa ttcattctgt tgatatgcaa agaatggggg
     3421 atatgtgggg ttcagggcta tttaaggcaa acgcatgtga ctattgtgat gatgttacaa
     3481 ccgaactcgc agacatatca ttgggtgatg cctggatttc gccatacaat atggatggag
     3541 ctggtaataa tgtagttgta tgtcgctcca ccacagctca tgaaattatt ttatctggga
     3601 ttgagaaaaa agacttagaa ttaacggaac tcgaattaga gcagttgaaa ttgtcacaac
     3661 aaggaagttt taaccacagg cataaaggat tattatatag aataaaaaat gcagagaaaa
     3721 ataatcgctt agtgcctgtt aagaggaaaa gattcttacg ctctatatct tttttattaa
     3781 agttaattca aaaacaacga tcagttacaa ggagaaaaag catagagata tggatggaaa
     3841 ctcaaaattc agcaactttc gataagaaaa tgaagggata tttattcacg ttgcgttggt
     3901 taacagtggt caataggaaa ttaagtcgga tgttcaagat tgtttctcta aatagaatgg
     3961 gtaaaaaatg aaagttgcaa tattaactca accattacat acgaattatg gtggcacatt
     4021 acaagcctat gcattacaaa aagtcttgat taatttggga catgaaccag agactattaa
     4081 ttataggtcg aaaattaaga ggccactatt tatccgtgtt gtgttgtcaa aaattaaaag
     4141 gatagtatta tgccgaaaaa tcacatttga ttttacaaca caagatagaa ttaatattag
     4201 gacgcatcat caatctttca ttgatacaag attaaactat tcagaggaga ttaacggtac
     4261 tgaggggttg agagattata ttttaaaaaa taattacggt gctgtaataa taggtagtga
     4321 tcagacatgg agaccaatat attcgcctag aatagattca ttttttctcg attttttaag
     4381 tgatgtaaac gatataaaaa aaatggcata tgcagcatct tttggtactg ataaatggga
     4441 gttcacggag tcacaaacta atttatttaa attgttactt tcaaaatttg actatgtttc
     4501 agttagagag tcctcaggag tcaaattatg tagtgaaaaa tttggtttga aagcagagtt
     4561 ggttttagat ccgacattgc tattgacatt cgaagattat actcatttat tagacaatga
     4621 ctatattaat cttcataagg gaaaggtgtt tagttatgta cttgatgaga attacgacaa
     4681 aaaaaactct atagacacag tagcaagtac tctccgcaca acgtcatttt atacttaccc
     4741 taaaaaaatc actaaagatg aatatgtgat tagaaattat tccgagtatg aatatccgcc
     4801 aatagaatat tggatttctt cgtttaaggc cgctgaattt attgtaacag attcatttca
     4861 tggaacagta ttttctatta tttttaacaa acccttcatc gccattgcaa atgaagagag
     4921 aggaaaggct agatttacgt cactattgga aatgtttggt cttgaaagac gacttgtgag
     4981 taatttagat gatgttgatt taaagcttgt aaatgaaaaa attaactatg gccccataaa
     5041 tgaaaaaatt gcttatttta gagaagagtg ccttaaaaag ttgcagttaa tgctaataaa
     5101 ataatcagat tatgtatgtc tctctacaat taaaatatga atgttgatcg atgaagaatt
     5161 gtaaagtatc tgtaattatt ccggtctata atgcggaaaa atatatacaa cgttgtattt
     5221 taagcttatt aaagcagaca ttagatgatg ttgaaattat catcattgat gatggttcta
     5281 ctgataattc actttctata ataaaagaaa ctgttgcatt gcatactgct agtagagctc
     5341 gccaaaagag aatcaatata atctctcgag aaaacaaagg agttgcctat acacgaagtc
     5401 agggattgag actttcgcag ggggaattta tcattcattt tgatagtgat gattgggcaa
     5461 aatccaatat gctggaggaa atgtataaga caattgtttc caataatgca gacatggtta
     5521 tatgcgatta ttttttagtg aagaataata aagaaatact tataaaacag agagtggagt
     5581 atgagccaaa gaaatgtatt agatatctac tgactggtga gctagaaggg tttacatgga
     5641 acaaactaat tagaaaaaaa tatatcgata aaaataaaat tgattttgtt aataaaatta
     5701 cttatatgga ggattttttg ttcatattaa gtgtgttact acacaatcct aaaattattt
     5761 ttcaggattg tgcatattat tattatcaaa aaagcaatcc taaatctcta actagccatg
     5821 ctagtgtgga taggctttct gaaatgatta aagccgtaag tgaaatagaa aagaagataa
     5881 ataaatataa tttacaaaag tatctgaata acgagtttca attgtttaag ttaaaacaaa
     5941 aaatttggtt tatatctata agtaagttaa atgttaatga taatgtatgg aatctctttc
     6001 ctgaaacaaa tccatttata tcaaaggtaa atgtgctttt ttattataaa atagtgcttt
     6061 ttcttgatag tattaaactg cgtttttttt caaataagat catttatctc attggattgg
     6121 tgcaggcttt attacaagaa aggaaagaga agtgatacca aaaataattc attactgctg
     6181 gttcggtcgc tctccattgt cggaattaac aaagcagtgc attgcaagct gggagaaata
     6241 ttgtcctgaa tataaaatca taagatggga tgaaaataat gtcgatttga attcttgttc
     6301 ttttgttaga caagcttata aagaaaaaaa atgggcgttc gtttctgatt atgtaaggct
     6361 taaagtggtt aatgaatatg gcggtatcta tcttgataca gatgttgaat taataaagcc
     6421 attagatgat ttgttaatat atcctgcata tataggcttt gaaattaaca aggaatggta
     6481 tgtaaattct ggtttagggt tcggttctgt taataataat ccggttttag aatcattaat
     6541 tatggagtat gaaaatatta attttgtaaa tgaggatggg actctaaata taacgccgtg
     6601 ccccataaga gaaacaaaag cattaacaaa aataggttta attcctgatg gtcaatgtca
     6661 atcttttgat aatatagtga ttttttctgc agattatttt tgtcctgtat ctataacagg
     6721 tgagagaaat ttttctgata aaacatattc aatacatcat tacgatgcta gttggttttc
     6781 tgagcaaaag agaaaaggcc tgcaacgtaa aaagagattt ataaaactat tcggcaacct
     6841 aataggcaca tatattaata aaccttttat ttttgtagat gaatgtcgcg agttcggatt
     6901 agttaaagca atcaagaata tgcgttccaa ttttccttaa tttttagtgt taaggaataa
     6961 gtttcgttaa aatactttat tcatttaatt aataaaggtt taatggtgat tagatgtact
     7021 ttcttttgtt cttctcaata ttttattttg tatacgtaat cttaatagga acattatgtc
     7081 ataactataa agcccttttg attttttcac taatccctct gacaatagtg tcaggtataa
     7141 gatataatgt cggctttgat tttatgtcat atgttgatta tttcaatcaa ctcaaatatt
     7201 caaatgatat atatctagat tctaccttta agtatataag ctactttacc tattatatag
     7261 gaggtaacga acagattttg tttctgatat atgcggtttt ttattcggtt gcactttatt
     7321 ttttaataaa actggtattg gaaaattata taacggaaat aaataatttt tatactattg
     7381 gacttgtgtt atcattttat tcgttttatt ttttattatc atttaatcaa attagggctg
     7441 tattatcagc tctaatacta tgttatgggc tattaaaaaa taaaatagat ttttctttta
     7501 taataacaat tacattaagt attctttttc actctgcagc aatgttcatt ttacctcttt
     7561 attttgtcct aagaaaaata aatgtaacag ttctgttggt gatattccct tttttggttg
     7621 ttgcttcatc ctttaatatt ttttcagata ttgtgagatt cattctgaca ttgctaaata
     7681 gtcgtttttt aacatatttt aactctgaat attttgttcc aaggacggga atggaaaaaa
     7741 tgtatagtat gatctcaatg attattgttt tgggaatggt tgtttgtttg tctaaattat
     7801 tacctaaaaa gtttgattta atgataaagt ttgttattct ttttgttcta cttagagcga
     7861 tgtcaattga tatattgatt tttgctaggt ttagtgattt tcttaaacca atggctataa
     7921 ttcttatatt taccgctatt tatttttctt cgaataaagt gcgacctaga attatccttc
     7981 ctttttattt aattatgaca ttgtttatct gtatttttaa tataataatc ggatcgaata
     8041 taactaagga gaactattat acttatggtt ataacatttg cttttttggc aataaatgta
     8101 tagataaatt ttattagtaa ttgtcgtttg atacttctgg tatttatgga aaaatatatg
     8161 aataactttt cttgtaaaga acccccttca tttattgaaa ataaaattat attatcaatt
     8221 attcttcctg tatataacgt atcagagtat ttaattgagt gcctaaacag tttgctgtta
     8281 gacgttcatg aacgttatct taatagatgt gaagtaattg tggttgatga tggttcaact
     8341 gataattcat ttgaattaat gagagagtat tctttgaaat atcctgaatc tattaagatt
     8401 tattcgaaat tcaatggtgg tctctcagat gcaagaaact atggattatt aaaatcttca
     8461 ggtaaatata tttcttttgt tgattcagat gatgttgtta atagaggttt tataaaggaa
     8521 atcatcaatt tcattgatgc ctatgatttt gatatattgt catttgactt catgaagttt
     8581 tttaataata atggtgttct tattctttca caaatagatg aattctccaa aatggcagaa
     8641 agagtagatt cggagttcta caagtccaag cctgtttttg cttggaataa agtttatcga
     8701 cgttctcttt tcgacaatga agtatttcct aagggatggt attatgaaga tgttgcatta
     8761 attccgttat tattagatag agcaaaagtt ttataccata ttaatagcgt ttgttatttt
     8821 tacagacaga gacaaggagc aatcactttt ttttatgaca ataaatatct tgatatttta
     8881 aaaggagtgt cttttttata tgacaggtca caatcttcat tcattaaaac gataattata
     8941 aatcagtttt ttactttaac cttactatct ttaagattgc cgactgataa ttatttcatg
     9001 aatatgaggg ggattattga tacctattgt gaaaaatttg acttggattc ctttgagcct
     9061 aaactaatat taaaacacat tcctttttta ttgcttaaaa aattgaagtc gctgtgtgtt
     9121 tatccactgt atttatttaa acctgcagtt tttttacata aacaaattaa acattttaga
     9181 ggaaaactaa agtgaaatat gattatataa ttataggtag tggtcttttt gggagtgtgt
     9241 ttgcacatga attagggaaa atgaaaaaaa aggtcaaggt tattgagcgt cgtgatcatg
     9301 ttggtggtaa tgtatattgt gagaatattg aaggaattaa tgttcataaa tatggcgcac
     9361 atatatttca tactaacgat aaagaaatat gggattatgt aaatcaattt gtagagttta
     9421 atagatatac aaattcacca ttagcttttt caaaaggtaa aatttataat ttacctttta
     9481 acatgaatac ctttaatcaa ctctgggggg ctattactcc tcaacatgcg aaagaaatta
     9541 ttaaaaagca aagcggtgaa ataagtggaa ataaaccaag gaatcttgaa gagcaagcta
     9601 tttctttggt gggacgagat atttataatt gtttgattaa ggagtacact gagaaacaat
     9661 ggggacgacc ttgcactgaa ttgccatcat ttattataaa acgactccct gttaggttta
     9721 catacgataa taactatttt aatgaccagt atcaaggaat accagtcggt ggttacaata
     9781 aattaatcga tgcattatta aatggtattg aagttcaaac atgcactgac tattttgaag
     9841 agaagaaata ttgggattct attgcaaaga aaattattta tacagggcct attgataagt
     9901 tttattgcta taaatttgga gctttggatt atcgatcatt aaaatttgag catgaattac
     9961 tgaatgtcga taattttcaa gggaatgctg ttgtaaatta tatagacaaa aaattcccat
    10021 tcactcgtat aatagaacat aaattttttg aatttggtga acagcataat actctcatta
    10081 ctcgagagta cccaaaagaa ttcgaagaag gcgatgagta ttactatcct gtcaatgatg
    10141 aaaaaaatat gcaaatctat aaaatgtatt ataatctttc taaacaggaa agtaatgtga
    10201 tttttggtgg acgtcttgcg gaatataaat attatgacat gcatcaagtt atcaaatcag
    10261 ctttaaatgc attccgaaaa gaggtacgtt taggtgaata aagaagtaat cgcagttatt
    10321 gtaacctata atagaaaagc attattgctg aaagttatag atgctgttat taaccaaagt
    10381 tatccactaa aaaaaatatt gataatagat aataatagta ctgatggtac ggagatgttt
    10441 atttcaaata ggctctctga cgtggtgaag tataaaaata cgggggataa tttagggggg
    10501 gctggcgggt tttatagagg attcatagag gcggaaaaat atggctatga ttatctttgg
    10561 ttaatggacg atgattgtat gcctacagtg aattgtcttg aaatattaat atcaaataac
    10621 cctgatggta ttgtccaacc tgttagatat aacttggatg aaacttgtgc agagctttct
    10681 ccattaactt atgatttatc taacccattt aagttaaacc ctaaaggtac accattaaaa
    10741 aattacttaa atacaattgg taataaatct gcgaaggtag atattgaagc tattccattt
    10801 gaaggtccat tgatttcacg aaaggttgtt gaaaaaatag gctatccaga gcctaagttt
    10861 tttatttttt gtgatgacat tgaatacgct attaaagcta aaagaaaggg gattcccatc
    10921 cagtgcaatc ttaaagccaa ggcataccgc cttttggtca acaatcaagg taatgatctt
    10981 ctttcctgga aaggatattt tatgcttcga aatcttttct acctgcataa aacgtatggg
    11041 accaatttcc ttgttagaca aaaacctatc gtattgggat taggatatgc attgtcgtgt
    11101 gtattgaaat gcaatttttc tcaacttccc gttatatggc gtgctttttg ggattcatct
    11161 acgttaagaa ataccgaaaa attcaggccc gaaatgagaa gtaaataatt tattattatg
    11221 tttttattaa ttaatgtcga tttttactaa aattcttaac acgatattta tccctgacag
    11281 gagtaaacaa tgtcaaagca acagatcggc gtcgtcggta tggcagtgat ggggcgcaac
    11341 cttgcgctca acatcgaaag ccgtggttat accgtctcta ttttcaaccg ttcccgtgaa
    11401 aaaacggaag aagtgattgc cgaaaatcca ggcaaaaaac tggttcctta ctatacggtg
    11461 aaagagtttg ttgaatctct ggaaacgcct cgtcgcatcc tgttaatggt gaaagcaggt
    11521 gcaggcacgg atgctgctat tgattctctc aagccatacc tcgataaagg tgacatcatc
    11581 attgatggtg gtaacacctt cttccaggac accattcgtc gtaaccgtga gctttctgca
    11641 gaaggcttta acttcattgg taccggtgtt tccggcggtg aagagggggc gctgaaaggg
    11701 ccgtccatca tgcctggtgg ccagaaagaa gcctatgaac tggttgcacc gatcctgacc
    11761 aaaatcgccg cagtagctga agacggggag ccatgcgtta cctatattgg tgccgatggt
    11821 gcaggtcact atgtgaagat ggttcacaac ggtattgaat acggtgatat gcaactgatt
    11881 gctgaagcct attctctgct taaaggtggc ctgaacctca ccaacgaaga actggcgcag
    11941 acctttaccg agtggaataa cggtgaactg agcagctacc tgatcgacat cactaaagac
    12001 atcttcacta aaaaagatga agacggtaac tacctggttg atgtgattct ggatgaagca
    12061 gcaaacaaag gtaccggtaa atggactagc cagagcgcgc tggatctcgg cgaaccgctg
    12121 tcgctgatta ccgagtctgt gtttgcacgt tatatctctt ctctgaaaga tcagcgcgtt
    12181 gccgcgtcta aagttctcac tggcccgaaa gcacagccag ctggcgaaaa ggctgagttc
    12241 atcgaaaaag ttcgtcgtgc gctgtatctg ggcaaaatcg tttcttacgc tcagggcttc
    12301 tctcagctac gcgccgcgtc tgaagagtac aactgggatc tgaactacgg cgaaatcgcg
    12361 aagattttcc gtgctggttg catcatccgt gcgcagttcc tgcagaaaat caccgatgcc
    12421 tatgccgaaa atccgcagat cgctaacctg ctgctggctc cgtacttcaa gcaaattgcc
    12481 gatgactacc agcaggcgct gcgcgatgtc gtcgcttacg cagtacagaa cggtatcccg
    12541 gttccgacct tcgccgctgc ggttgcctat tatgacagct accgttctgc tgttctgcct
    12601 gcgaacctaa tccaggccca gcgcgacta
//
