LOCUS       EU296402               14737 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Shigella dysenteriae type 4 O-antigen gene cluster, complete
            sequence.
ACCESSION   EU296402
VERSION     EU296402.1
KEYWORDS    .
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14737)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 14737)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..14737
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:622"
                     /note="type: 4"
     gene            1081..2352
                     /gene="wzx"
     CDS             1081..2352
                     /gene="wzx"
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ACD36980.1"
                     /translation="MFRQINFKNLEIMKQILISFSFRLMGPVIMLLTTPQLLSTLGNV
                     EYGVWSLLITTISWLTICDFGISNQIRNESKILIVQDNKESIREIYTAAVIISFFITL
                     LCCVIISFINIDLIYKLFLSCVFLFAIETNICRALCLSLNKPELASAVTNIPNLFLLI
                     YVFCYSSFNINNVMDIYCYGSAMMFVSIFIILYKFNIKLFWPVFHPSYLRFSIMKYSL
                     LRGGGGFFMVQLVSMGLLISDRYIISSIFGVEDVTKYDLIFKVSNFCIMFFSIVNMVL
                     WSKFTEFWMQGFYTKIKVIFKVFDLCFLLLVIVLILILNHIHWIFELWLNVTNENYNE
                     VYITGIVLYLVGYYWMSAYSSFLNGISIIKKQFIIQGGGLLIKAITLLIAMNILKVFK
                     VEYIILLGGACLFIVGFLFRKCAHDKINSCI"
     misc_feature    1081..13270
                     /note="O-antigen gene cluster"
     gene            2327..2932
                     /gene="wfeU"
     CDS             2327..2932
                     /gene="wfeU"
                     /note="acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeU"
                     /protein_id="ACD36981.1"
                     /translation="MIKSIVVYSFRIYYFINRWITTKINKLRLKANSVELGDSVMING
                     ILHIVNKGEIKIGNGVKINSSLRYNPISHHPCSFYTSENAVIEIGDKSGLSGCTLYSV
                     KKIHIGKRVLIGAGVNIYDTDFHSLLVDERLNGDSNIKSSPVYIYDDCFIGANVTILK
                     GVTIGAGSVVAACSVVTKNIGENELWAGNPARYIRDTIYGE"
     gene            2874..3650
                     /gene="wfeV"
     CDS             2874..3650
                     /gene="wfeV"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD36982.1"
                     /translation="MNYGQAIQLGILEILYMANDKITVITINYNNAEGLSKTIASVEK
                     QTNKEFEYLIIDGGSEDNSIEHAKKSVICTKIISEKDNGIYDAMNKGASLAKGEYIIF
                     TNSGDELRKNSIEILMNVLRETNTLYDIYHGILAFKKNDKVIYYRGRKSDMLNSSMIE
                     HPTVLMRKSTFNELKGFDNKWRYVADYDLMLRAYFTKRRFFFIEEVIADFDVSGVSSV
                     SIKAPLESLRLQRKYNLINTFEYFSKLFFIKMKKAVSCLR"
     gene            3638..4753
                     /gene="wfeW"
     CDS             3638..4753
                     /gene="wfeW"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD36983.1"
                     /translation="MLKVILLSSINPEQKTGYYNAVNDRVKSLKKEKGIKIWSYSIYK
                     STSLIRFSIKKNENHFFIEYPKAFPSHGLFSFITLWFIRCVILFLWVIIRPNMIHVHW
                     GYPLAYAAVIIKRLTKKTKVIVTFHGSEVHTQPARSKEIFVKTCNLCKRVDHVTAVSE
                     NLLLQVRNKFSVSESKSSITYNGVNLDETNCSFSSSNGLTFSFFGNLNMTKGADRIIP
                     LYNALSGLIEENFTFYVGGEGPCYKEIKQYIIVNGISNIKLLGFMPRADVKRIMRKSN
                     CVFILSRNEGLGISAIEALAYSQLCIAPDIGGLSEVFRENPELLIKNDFNPSDYANKF
                     ISCHKDKFRVNRTYIFKNFSMKNIIQKEITLYNEIMK"
     gene            4769..6001
                     /gene="wzy"
     CDS             4769..6001
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ACD36984.1"
                     /translation="MNCKFNIWDIQLGLILISVLSYAFLNLGFLPLNISHLIILFVLI
                     TLSVNLLISQSIKITGLFLTLFVSFILLSAYSLLKYYDVQKVKNIINFFIFFSVAAIV
                     INNCSADKIIKFYYKLTKIFIFFALLQWVLYYLNIGTLYTYSFLGLKEVNISTSGYLI
                     RLFSIASEPAALCGILLPAIYLSINRIVNKGKEVTLSYSVIVLFVILNTFSLVGYIYI
                     IICLIVALYVGNKISLSKIFIFTICLALLVFILFQSDSIQQRLNEITSLDKMASSDNL
                     SVIAIYSNLQIALISLSDNLIFGGGIFSHPYTYDHYISQLYAGGGPRMELNKDDAASL
                     YIRALSETGILGFCILNGLIIYLMKRCDKSKINYPYNIAFTIAFALLGIRAGSVNYII
                     IWFYFFSAIRFVNEGRLK"
     gene            5998..7119
                     /gene="wfeX"
     CDS             5998..7119
                     /gene="wfeX"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD36985.1"
                     /translation="MKMLSFLECMNSTYGGPAFSLPSLLHHLEKNYLVQSTVISLKKK
                     NEVYENNVAECLGINPIILNQVGPRKLKLTHEIAKVKKIMQDCDIIHTNNLWNYFSYL
                     PYNLSKNSKKPNVISVRGSLYAWSLEQSRLLKKISWFLFQKKALDSAGFIHVTCNDEL
                     TALRELGIKNKIVVSQHGVDVPDLIDKVPDSFYDKYKLSHNKKYFLFMSRLHTKKGLD
                     MLFNVWSRISHHYPDWELLVAGPDYGNYIPKITKLNAVKYMGMLSGEEKEYCFSIAQF
                     FVLPSYTENFGVVIGEAMARTVPVLTSTNTPWKDVNTNNAGVCFDLSTENLKRELEFF
                     FNLSDSTRKEMGRNARELIVRNYSWERVSYPFYDALKSL"
     gene            7131..8249
                     /gene="gmd"
     CDS             7131..8249
                     /gene="gmd"
                     /note="GDP-mannose-4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gmd"
                     /protein_id="ACD36986.1"
                     /translation="MKKALITGITGQDGSYLAEFLLDKGYEVHGIKRRASSFNTERVD
                     HIYQDRHNQNPNFFLHYGDLTDTSNLIRLIKEIQPDEVYNLGAQSHVAVSFESPEYTA
                     DVDAMGTLRLLEAIRICGLEKKTRFYQASTSELFGLVQEIPQRETTPFYPRSPYAVAK
                     MYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANISQGIEKCLYLG
                     NMDSLRDWGHAKDYVRMQWMMLQQDHPEDFVIATGKQISVREFVRMSAKEVGLELEFS
                     GQGVDEIATVVNKTSDCAIGVSIGDVIVRVDPRYFRPAEVETLLGDPAKAKKVLGWEP
                     EITVEEMCAEMVASDLAKAKQHALLKSHGYDVAVSLER"
     gene            8253..9218
                     /gene="fcl"
     CDS             8253..9218
                     /gene="fcl"
                     /note="GDP-L-fucose synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="Fcl"
                     /protein_id="ACD36987.1"
                     /translation="MTKKRIYVAGHRGMVGSAICRQLSLRDDIELVVKTHKELDLTVQ
                     KEVDAFFEQEKIDQIYLAAAKVGGIYANNTFPAEFIYQNLMIESNIIHSAHKAGIQKL
                     LFLGSSCIYPKFAEQPMNESALLTGILEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGINDNFHPENSHVIPALMRRFHEAKESGAPEVIVWGTGTPMREFLYVDDMA
                     AASVHVMELDEAIYQQNTQPMLSHINVGTGVDCSIREMAETMASVVGYQGKIVFDVTK
                     PDGTPRKLMDVTRLKNLGWQYQYNLHEGLSLTYKWFIENINSFRG"
     gene            9221..9682
                     /gene="gmm"
     CDS             9221..9682
                     /gene="gmm"
                     /note="GDP-mannose mannosyl hydrolase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gmm"
                     /protein_id="ACD36988.1"
                     /translation="MNKRLERELFKTIVEHTPLISIDLIIRNDKGEALLGQRLNRPAQ
                     NYWFVPGGRIYKDESFEIAFKRITFEEVGVQISLNDALFLGVYEHFYNDNFSEAEFST
                     HYVVHGYEIQLKPQQLHLPTVQHNSYKWFDVVTLLNSTTVHQYTKNYFIPR"
     gene            9688..11094
                     /gene="manC"
     CDS             9688..11094
                     /gene="manC"
                     /note="pyrophosphorylase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManC"
                     /protein_id="ACD36989.1"
                     /translation="MLLPVVMAGGSGTRLWPLSRTLYPKQFLSLNSRLTMLQETLRRL
                     DKVEHKPALVICNESHRFIVAEQLRKEGLKHSGILLEPVGRNTVPAVALAALQAMVTG
                     DDPILLVLAADHEIQNEDNFIDAVLAAKNFAEQGKLVTFGIVPTSPETGYGYIKSGEY
                     LDGKGYKVAAFVEKPELHVAQQYISDGGYLWNSGMFMFRASVFIDELKKFRPDILASC
                     QRSLSSSIQDLDFIRLDNASFSCCPEESIDYAVMEKTAEAVVVPLNAQWSDVGSWSAL
                     WEISSKDQSGNAIRGDVLVEDATDSYLYSQHRLIGAVGIKDLVVVETKDAVLVAHKDK
                     VQQVKNIVAQLKKNNRTEYLQHREIFRPWGSHDTIAEGPRFQVKHVIVLPGHITAKQI
                     HYHRTEHWIVVSGTAKVHLEDKTYLVSENESTYIPVGVPHAIENPGKIPLEIIEVRSG
                     VYLEEDDVIRVSSSGVGY"
     gene            11094..11840
                     /gene="wfeY"
     CDS             11094..11840
                     /gene="wfeY"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD36990.1"
                     /translation="MRISIITATYNSEKTLLDTLLSLEKQTYPDIEYIVVDGASNDNT
                     IKLIKSNCTKVSKIICEPDKGIYDALNKGIQAASGDVIGFLHSDDLLAYDDAIADIAK
                     TFEITGCDAIYGDLEYVAQNNTTKRIRLWKSGSFSRFKMKVGWMPPHPSFYMKRKCYR
                     QFGCFSLDYRISADYDSLLRYILKQRISIEYLPKVLVKMRVGGISNRSVSSMVKKSME
                     DIRVMNQNGIIWPIALVYKNISKLPQFIKK"
     gene            11846..13270
                     /gene="manB"
     CDS             11846..13270
                     /gene="manB"
                     /note="phosphomannomutase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManB"
                     /protein_id="ACD36991.1"
                     /translation="MLNVKKIINDSNIAFGTSGARGLVIDFSHDVCAAFTHAFLSVID
                     DKYNFNKVALAIDNRPSSYEIAQACAFAIKQHGFTVEYHGVIPTPALAHYSMQKNIPC
                     IMVTGSHIPFDRNGLKFYRPDGEITKEDELAIINSEYTFPPVGVLPHLETSTQGADYY
                     LERYVSLFNPEILKGKRIGVYEHSSAGRDLYAPLFNQMGAEVISLGRSDEFVPIDTEA
                     VSDEDRILAREWSKKYNLDAIFSTDGDGDRPLVADENGEWLRGDILGLLTAIELNIKA
                     LAIPVSCNTAIEESNKFASIQRTKIGSPYVIAAFADLAKQFDSVAGFEANGGFLLASD
                     LQINGKELKSLPTRDAVLPALMLLIASRNSTISQLINNLPQRFTWSDRVKNFPSDSSQ
                     QIIKNAISSPNNFFNSLGYESLSCSAIDETDGARFTLNNGDIIHLRPSGNAPELRCYA
                     EASDENQAKQYVTNVLGNITSLIS"
BASE COUNT     4537 a   2420 c   3029 g   4751 t
ORIGIN
        1 attgtggctg cagggatcac agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc atatgaatta gaatctctcc ttgagcagcg cgtgaagcgt
      121 caactgcttg cggaagtgca gtccatctgt ccaccgggcg tgacaattat gaacgtgcgt
      181 cagggcgaac ctttaggttt aggccactcc attttgtgtg cacgacccgc cattggtgac
      241 aacccatttg tcgtggtgct gccagacgtt gtgatcgacg atgccagcgc cgacccgctg
      301 cgctacaacc ttgcagccat gattgcgcgc ttcaacgaaa cgggccgtag ccaggtgctg
      361 gcaaaacgta tgccgggcga tctctctgaa tactccgtca ttcagaccaa agaaccactg
      421 gatcgtgaag gtaaagtcag ccgcattgtt gaatttatcg aaaaaccgga tcagccgcag
      481 acgctggact cagacatcat ggccgttggt cgctatgtgc tttctgcaga tatttggccg
      541 gaacttgaac gcactcagcc aggtgcatgg ggacgtattc agctgactga tggcattgcc
      601 gaactggcga aaaaacagtc tgttgatgcc atgctgatga caggtgacag ctatgactgc
      661 ggtaaaaaaa tgggttatat gcaggcattt gtgaagtatg gactacgcaa cctgaaagaa
      721 ggggcgaagt tccgtaaagg gattgagaag ctgttaagcg aataatgaaa atctgaccgg
      781 atgtaacggt tgataaggaa attataacgg cagtgaagat tcgtggcgaa agtaatttgt
      841 tgcgaatatt cctgccgttg ttttatataa acaatcagaa taacaacgag ttagcaatag
      901 gattttagtc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtttgaa tttatgaggg ctttgcgggg ttagatgcag agttcgtgac atccattgag
     1021 aatctacgca gtgcactggt agctgttaag ccaggggcgg tagcgtgtgc aaataagaat
     1081 atgttccgac aaataaattt caaaaatcta gaaataatga aacagattct aatttcattt
     1141 tcttttagat taatgggtcc tgttatcatg cttctgacca cgccacaatt gttgtcgact
     1201 ttgggaaatg tggaatatgg agtttggtct ttattgatta caacgattag ttggttgaca
     1261 atatgcgact ttggcatatc aaatcagata cgaaatgaaa gtaagatttt aattgttcaa
     1321 gataataaag aaagtatacg agagatatat acagcggcag ttataatttc attctttata
     1381 acattattat gttgtgtgat tataagtttt attaatatcg acttgatata taaattgttt
     1441 ttatcttgtg tttttttatt tgctatagaa acaaatattt gtagagccct atgcctttca
     1501 ttgaataaac ctgaattggc ctctgcagta actaatattc cgaatttatt tttattgata
     1561 tatgtgtttt gttattcgtc atttaacata aacaatgtga tggatatata ttgttatggc
     1621 tctgccatga tgttcgtatc aatttttatt attttatata aatttaatat taagttgttt
     1681 tggccagttt tccacccttc ttacttaaga ttttctatca tgaaatactc tctacttaga
     1741 ggtggtggtg gtttctttat ggttcaattg gtttcgatgg ggttattaat aagtgataga
     1801 tatataatat catctatatt tggtgttgaa gatgtcacaa aatatgattt gatatttaag
     1861 gtgtcaaatt tttgcataat gtttttttca attgtaaata tggttctttg gtcgaaattt
     1921 acagaatttt ggatgcaagg gttttataca aaaatcaagg ttatctttaa agtgtttgac
     1981 ttatgttttt tattgttagt tatcgtccta attttgattt taaaccatat acactggatt
     2041 tttgaattat ggctaaatgt aactaatgaa aattacaatg aagtttatat tacagggatt
     2101 gtcctttatt tggttgggta ttattggatg tcagcttatt cctctttttt aaatggtata
     2161 agcatcataa aaaaacaatt tattatccag ggtggagggc ttttgatcaa agctattact
     2221 ttattaattg ctatgaatat cttgaaagta tttaaggttg aatatattat tttgctgggt
     2281 ggtgcgtgtc tatttattgt tggtttttta ttcaggaagt gtgcgcatga taaaatcaat
     2341 agttgtatat agctttcgga tttattattt catcaataga tggattacaa caaaaataaa
     2401 taaattgagg ttgaaagcta actctgtcga attaggtgac agcgtaatga ttaatggaat
     2461 attgcatata gttaacaagg gggaaattaa aattggtaac ggtgtcaaga ttaattcttc
     2521 attacgttac aatccaattt cacatcatcc atgttcattt tacacttctg aaaatgcggt
     2581 gattgagatt ggggataaat ccgggttgtc gggttgtacc ttatattctg ttaaaaaaat
     2641 ccatattgga aaaagagtgt tgattggtgc aggcgtaaat atttatgata cagattttca
     2701 ttctttactc gtggatgaga gattaaatgg tgatagtaac attaaatcct ctccagttta
     2761 tatatacgat gattgtttta ttggagctaa tgttacaatt ttgaaaggtg ttacaatagg
     2821 ggctggcagt gttgttgctg cttgctcggt agtaacgaag aatatagggg aaaatgaact
     2881 atgggcaggc aatccagcta ggtatattag agatactata tatggcgaat gataaaatta
     2941 ccgttataac tataaattac aataatgcag aaggcttatc aaaaacgatt gcttcagttg
     3001 aaaagcaaac taataaagaa tttgagtatc taattattga tggtggttct gaggataatt
     3061 caatagaaca tgctaagaaa agtgtaatat gcacaaaaat aatatctgaa aaagataatg
     3121 gaatctatga tgcaatgaat aaaggagcct cacttgctaa aggtgagtat atcattttta
     3181 caaactctgg tgatgaatta agaaaaaata gcattgaaat tttgatgaat gtattgagag
     3241 agacaaatac attatacgat atataccacg ggattttggc tttcaaaaaa aatgataaag
     3301 ttatttatta tagaggtcgg aaaagtgaca tgttgaacag ttcaatgatt gaacatccta
     3361 ctgtgttaat gcgtaaaagc acatttaatg aattaaaagg atttgataat aagtggcgat
     3421 atgtagctga ttatgattta atgttacggg catattttac aaaaagaaga ttttttttta
     3481 ttgaggaggt gattgctgat tttgatgtat caggtgtatc tagtgtgagt attaaagctc
     3541 ctttggagtc tttacggtta caacggaaat ataatttaat taatacattt gaatatttta
     3601 gcaagttatt ttttatcaag atgaaaaagg ctgtatcatg cttaaggtaa tccttctttc
     3661 atcaatcaac ccagaacaaa aaacaggcta ctataatgct gtcaatgacc gtgtaaaatc
     3721 tctaaaaaaa gagaaaggaa taaaaatttg gtcatattcg atatataaaa gcacgtcatt
     3781 gataagattc tcaataaaaa aaaatgaaaa tcattttttt atagaatatc ccaaagcatt
     3841 tccttcgcat ggtttatttt cattcataac tctctggttt ataagatgtg ttattttatt
     3901 tctgtgggtt ataataagac ctaacatgat acatgtgcat tggggatatc cgcttgcata
     3961 tgctgctgta attataaaaa gattaaccaa aaaaacaaaa gtcattgtga ctttccatgg
     4021 ctctgaagtc catacccagc ctgccagatc gaaagaaatt ttcgtaaaaa cttgtaattt
     4081 atgtaaaaga gttgatcatg ttacggctgt gagtgagaac ttattattgc aagtaagaaa
     4141 taagttttca gtgtcagaat cgaaatcatc tataacatat aatggagtta acctagatga
     4201 aactaattgt tctttttcat caagtaatgg attgacgttt tcgttttttg ggaatctaaa
     4261 tatgactaaa ggtgctgata ggataattcc actttataat gcactttcag ggttaattga
     4321 agagaacttt acattttatg ttggcgggga aggaccttgt tataaggaaa taaagcaata
     4381 tattatcgta aatggaatct ctaatattaa attgctcggt tttatgcctc gtgctgatgt
     4441 aaaacgaata atgcgcaaaa gtaattgtgt ctttatatta agtcgtaatg aagggttggg
     4501 tattagtgca attgaagcat tagcttatag tcaattatgt atagcacctg atataggtgg
     4561 attatctgaa gtttttagag agaatccaga gttattaatt aaaaatgatt ttaatccatc
     4621 agactatgct aataagttta ttagttgtca caaagataaa ttcagagtta atcgtacata
     4681 tatatttaaa aacttttcta tgaaaaacat aattcagaaa gaaataacct tatataatga
     4741 aataatgaaa taatgaaaca ggcacaaaat gaattgtaaa tttaacattt gggatattca
     4801 gttaggtttg atattaatat cagtactatc ttatgctttt ttaaacttgg gttttttacc
     4861 attaaatatt tcccatctaa taattttatt tgtgttgata acgctaagtg ttaatctgtt
     4921 aattagccaa tcaattaaaa taactggatt atttttaact ctttttgtgt cctttatttt
     4981 actttctgct tattcattat tgaaatatta tgatgttcaa aaagtaaaaa atataattaa
     5041 tttttttatt ttttttagcg ttgctgctat agtgataaat aactgcagtg ctgataaaat
     5101 aataaaattt tattacaaat taactaaaat ttttatattt tttgctttac ttcagtgggt
     5161 tctttattat ctcaacattg gaacactata tacatattct tttcttggtt taaaggaagt
     5221 taatatttca acaagtggat atttaattcg gctattctct atagctagtg agcctgctgc
     5281 attatgtggg atattgcttc cagctatata tctatctata aatagaatag ttaataaggg
     5341 taaagaagtt actttatcat attcagtaat tgttttattt gttattttaa atacattttc
     5401 gttggttggc tatatatata taatcatttg tttaatagta gcgctatatg taggtaataa
     5461 aatctcatta agtaaaatct ttattttcac aatttgtttg gcattgcttg tctttattct
     5521 ttttcaatcg gatagtatcc agcagcgttt aaatgaaata actagtctag ataaaatggc
     5581 tagctcagac aatctttctg ttatagcaat ttattcaaat ttgcaaatag ctttgataag
     5641 tctcagtgat aatcttatat ttgggggagg aatatttagt catccttata catacgatca
     5701 ttatatcagt caattgtatg ctggtggagg accacgcatg gaattaaata aggatgatgc
     5761 agcttctctt tacatacgtg cattatcaga aacgggaatt ttaggttttt gtattttgaa
     5821 tgggttgatt atatatctga tgaaacgctg cgataaatct aaaatcaact atccatataa
     5881 catcgctttt acaattgcat tcgcattgct tggtattaga gctggtagtg ttaattatat
     5941 aatcatttgg ttctattttt tttccgctat acggttcgta aatgaaggaa ggttaaaatg
     6001 aaaatgttat cgtttttgga atgtatgaat agcacttatg gagggccagc attttcactt
     6061 ccttctttac ttcatcattt agaaaaaaac tatcttgttc aaagtacagt tatttcgctt
     6121 aaaaagaaga atgaagttta tgaaaataat gttgctgaat gtttgggaat aaacccgatt
     6181 atattaaatc aggttggccc aagaaagtta aaattaacac acgagatagc aaaagtaaaa
     6241 aaaataatgc aagattgtga tataattcat acgaacaact tgtggaatta tttttcttat
     6301 ttgccctata atttatcaaa aaacagtaaa aagccgaatg ttatttccgt cagaggcagt
     6361 ttatatgctt ggtctcttga acagtcgaga ctgttaaaaa aaatctcatg gtttcttttt
     6421 caaaagaaag cattggattc agccggtttt atacatgtta cttgtaacga tgaattaaca
     6481 gctttacgag aattaggtat taaaaataaa atagttgttt cacaacatgg tgtggatgta
     6541 cctgatttga tagataaagt tccagatagt ttttatgata aatataaatt atcgcataat
     6601 aaaaaatatt ttctatttat gtctcgacta catacgaaaa aagggctaga tatgttgttt
     6661 aatgtatggt cgaggatctc tcaccattat cctgattggg aattacttgt tgctggtcct
     6721 gattatggaa actatatacc aaaaataact aagttaaatg cagtaaaata tatgggaatg
     6781 ctaagtgggg aagagaaaga atactgtttt tctatagctc aattttttgt tctaccttca
     6841 tacactgaaa attttggcgt tgtaattggt gaggctatgg caagaaccgt acctgtttta
     6901 acttctacta atacaccatg gaaagatgtt aataccaata atgcaggtgt atgtttcgat
     6961 ttatcaactg aaaatttaaa aagagaattg gagttttttt tcaatttatc tgattcaact
     7021 agaaaagaaa tgggaaggaa tgcgagagaa ttgatagtta gaaactattc atgggaacga
     7081 gttagttatc ctttttacga tgcacttaaa tcactttgag gtttaataga atgaaaaaag
     7141 cattaatcac aggcatcacc ggtcaagatg gatcttactt agcagaattc cttcttgata
     7201 aagggtatga agtgcatggc attaaacgtc gagcatcttc atttaatacg gagcgtgttg
     7261 atcatattta tcaagatcgc cacaatcaaa atccaaattt ctttcttcat tacggtgatt
     7321 tgacagatac ttcgaacttg atacgtctca ttaaagagat ccaaccagac gaagtttata
     7381 accttggcgc tcaatctcat gtagcggttt cattcgaatc acctgaatat actgctgacg
     7441 tagatgcaat ggggacttta cgtctgctgg aagcgattcg tatttgtggg ttagagaaaa
     7501 aaacacgctt ctatcaggca tctacttctg aactcttcgg cttagttcag gaaatcccgc
     7561 agcgagaaac aacaccattc taccctcgtt cgccttatgc tgttgctaag atgtatgcat
     7621 actggattac tgtaaactat cgtgaatcct atggaatgta cgcctgtaat ggtattctct
     7681 tcaaccatga atccccgcgt cgtggtgaaa cattcgtaac acgtaaaatc acgcgtgcta
     7741 ttgcaaatat ttcgcaggga atcgaaaaat gtctctatct tggcaatatg gattcactgc
     7801 gcgactgggg acatgcgaaa gattacgtgc gtatgcagtg gatgatgttg cagcaagatc
     7861 acccagagga ttttgttatt gcaacaggca aacaaatttc cgtacgtgaa tttgttcgta
     7921 tgtcagctaa agaagtcggt ctggagttag aattctccgg acaaggtgtt gatgaaatag
     7981 caactgttgt gaataaaaca tctgactgtg ctattggtgt tagtattggg gatgtgatcg
     8041 ttcgtgttga tcctcgttat ttccgtcctg cagaagttga aactcttctt ggtgatccag
     8101 caaaagcgaa aaaagtattg ggttgggaac cagagattac agttgaagag atgtgtgcag
     8161 aaatggttgc cagtgatctg gcgaaagcaa aacagcatgc actactgaaa agccatggtt
     8221 acgatgttgc agtttctctg gagcggtaag gtatgacaaa aaaacgtatc tacgttgctg
     8281 gtcaccgagg tatggttggc tctgctattt gccgtcaatt atcactgcgt gatgatatcg
     8341 aattagtggt caaaacacac aaagaactcg atctaaccgt acagaaggaa gttgatgcat
     8401 tttttgagca agagaaaatt gatcagattt atcttgctgc ggctaaagtc ggtggcattt
     8461 atgccaacaa tacatttccg gcagaattca tctatcagaa tctcatgatt gagagtaata
     8521 ttattcattc agctcacaag gccggaattc aaaaattact ttttttaggc tcaagctgta
     8581 tttatcctaa attcgcagag cagccgatga acgagtctgc gcttttaacg ggcatacttg
     8641 agccaactaa tgagccatac gcaattgcta aaattgcggg cataaaatta tgtgaatctt
     8701 ataaccgtca atatggccgt gattatcgca gtgtaatgcc tactaatctt tatggcataa
     8761 atgataattt ccaccccgaa aactctcatg ttattccggc actcatgcgt agattccatg
     8821 aggcaaaaga gagtggtgca ccagaagtta ttgtctgggg aacaggaaca ccgatgcgtg
     8881 agtttttata tgttgatgat atggctgccg catctgttca tgtaatggaa cttgacgaag
     8941 caatttatca acaaaataca cagcctatgt tatctcatat taatgttggt acgggtgtag
     9001 attgttccat acgtgaaatg gctgaaacaa tggcctctgt ggtgggttat caaggtaaaa
     9061 ttgtttttga tgttactaaa cctgatggca ctccgcgcaa acttatggac gttacccggc
     9121 tcaaaaactt gggctggcaa tatcaatata acttacatga aggcttatca ttaacatata
     9181 aatggtttat tgagaatatt aattcttttc ggggatagtt atgaataaga gattggaacg
     9241 tgagctattt aaaacaatag ttgagcatac tcctctaatc tcgattgatc tcataattag
     9301 aaacgataaa ggagaggcgc tgctggggca gcgcctgaat cgcccagcac aaaattattg
     9361 gtttgtgcca ggagggcgaa tttataagga tgagtcattc gagattgcat ttaagcggat
     9421 aacatttgaa gaggtgggcg ttcaaattag tcttaatgat gccttatttc taggggtgta
     9481 tgaacatttt tacaatgata atttttctga agcagaattt tctacacact atgtagtgca
     9541 tggatatgaa atccaactta aacctcagca acttcaccta ccaacggtcc agcataattc
     9601 ctacaagtgg tttgatgtag taacgttgct taatagcact acagttcatc aatataccaa
     9661 aaattatttt ataccaaggt aatagatatg ctacttcccg ttgtcatggc cggtggttct
     9721 ggtaccagat tatggcctct ttcacgtaca ctttatccga aacaatttct gtctttaaat
     9781 agtcgtttaa ccatgttgca agagacattg cggcggcttg acaaggtaga acataaaccc
     9841 gctttggtca tttgtaacga atcacatcgc tttatcgttg ctgaacaatt gcgtaaagag
     9901 ggtttaaagc atagcggtat tttgcttgag cctgttggtc gtaatactgt gcctgctgta
     9961 gcactcgcag cactacaggc tatggtaact ggagatgatc ctattctgtt ggttcttgct
    10021 gctgatcatg aaattcagaa tgaggataat ttcattgatg cagttcttgc agccaagaat
    10081 tttgcagaac agggtaagct tgttacgttt ggtattgttc caacatcccc agaaacgggc
    10141 tatggttaca ttaagtcagg tgaatatctg gatggaaaag gttacaaagt tgctgctttt
    10201 gttgaaaaac cagagcttca cgtagctcag cagtatatat cagatggcgg ttatctttgg
    10261 aatagtggga tgtttatgtt cagagcatct gtatttatcg atgaactgaa aaaattccga
    10321 ccagatattt tagccagttg ccaacgctcc ttgtcctctt cgatacaaga tttagatttt
    10381 atccgtctgg ataatgcttc attttcttgc tgtcctgaag agtctattga ctatgcagtt
    10441 atggaaaaaa cagccgaagc tgtcgtcgtt ccattaaatg cacaatggag tgatgttggg
    10501 tcatggtctg cattgtggga aataagttca aaagatcaaa gcggtaatgc cattcgtggt
    10561 gatgtattgg ttgaagatgc tacagatagt tatctttatt cgcagcatag acttattggt
    10621 gccgtgggca taaaggattt ggttgttgtt gaaacgaaag atgccgtatt agttgctcat
    10681 aaagataaag ttcagcaagt taaaaacatc gtcgctcaac ttaaaaagaa taatcgcaca
    10741 gaatatttac agcatcggga aatatttcgg ccttggggca gtcatgatac tatagctgaa
    10801 gggccgcgct ttcaagtgaa acatgtgatt gtattacctg gccacattac cgctaagcaa
    10861 attcattacc atcgtactga gcattggatt gttgtctcgg ggacagctaa agtccatctt
    10921 gaagataaga cttaccttgt ttctgaaaat gaatcaacat atatacctgt tggtgttcca
    10981 catgccattg aaaatcctgg caagatcccg cttgaaataa tcgaggtcag atcaggagtc
    11041 tacctggaag aggatgatgt tataagagtt tcttcttctg gagtaggata ctaatgcgaa
    11101 tttctattat cacagctact tataatagtg aaaaaactct ccttgataca ttactttctc
    11161 tagaaaaaca aacgtatcca gatatcgaat atatagttgt agatggagca tcgaacgata
    11221 atacaattaa gctgattaag agtaattgta caaaagtttc aaaaatcatt tgcgaacccg
    11281 ataagggcat ttatgatgct ctgaataaag gtattcaagc agcttcgggt gatgttattg
    11341 gttttttaca ttctgatgat ttattagctt atgatgatgc tattgcagat atagcaaaaa
    11401 catttgaaat tacagggtgt gatgctattt atggcgattt ggagtatgtt gcccaaaata
    11461 atacgactaa acgtattaga ttatggaaaa gtggttcatt tagtcgtttc aagatgaaag
    11521 tgggttggat gccgccacac ccatcatttt atatgaaacg caaatgttat aggcagtttg
    11581 gttgtttttc tttagattat cgtatatctg ctgattatga ctcattatta cgatatattt
    11641 taaagcaacg tatttcaata gaatatttac caaaagtctt agtgaagatg cgtgttggtg
    11701 gaattagcaa tcgttcagtc tcttcaatgg taaagaagtc aatggaagac atccgtgtta
    11761 tgaaccagaa tggtattatc tggccaatag ctttagtgta taaaaatata tccaaacttc
    11821 ctcaatttat taaaaagtaa tcatcatgtt aaatgttaaa aaaatcatta acgatagtaa
    11881 tattgcgttc ggtactagtg gtgctcgcgg tttagtgatc gatttttccc atgatgtttg
    11941 tgctgcgttc actcatgcgt ttctttctgt tattgatgac aaatacaatt ttaataaagt
    12001 tgccttagca attgataacc gcccaagcag ttacgaaatc gctcaggcat gcgccttcgc
    12061 tatcaaacaa catgggttca ctgtcgaata tcatggtgta attccgactc ccgcattagc
    12121 tcattattcg atgcagaaaa acattccctg cataatggtc acagggagtc atatcccttt
    12181 tgatcgtaat ggcttaaaat tctacagacc tgatggtgaa atcactaaag aggatgagct
    12241 cgcaattata aatagtgagt atacatttcc gcctgtaggt gtattacctc atcttgaaac
    12301 aagcactcaa ggtgcagact actaccttga gcgttatgtt tctcttttta atcccgagat
    12361 tttaaagggg aaaagaatag gggtatatga acattctagt gcgggacgcg atttatatgc
    12421 tcctcttttt aatcaaatgg gtgcagaggt catttccctc ggcaggagtg atgagttcgt
    12481 tccgattgat acggaagcag taagcgatga agatcgtata cttgcaagag agtggtctaa
    12541 aaaatataat cttgatgcta ttttctcaac agatggcgat ggtgatcgtc ctttagttgc
    12601 tgatgaaaat ggtgaatggt taagaggcga tattctggga ctacttactg ctattgaact
    12661 taatatcaag gcgttggcta ttccagtgag ttgtaataca gcaattgaag agtctaataa
    12721 atttgcaagt atacaacgaa cgaaaatagg ttctccttat gtaattgcag catttgcaga
    12781 tcttgctaag caatttgatt cagtcgcagg ttttgaagct aatggtggtt ttctccttgc
    12841 ctccgattta caaattaatg gcaaggaatt aaaatcgttg cctacacgag atgctgtgtt
    12901 accagcatta atgctcttaa tagcttctcg caatagtact atttctcaac tgattaataa
    12961 tcttcctcag cgattcactt ggtcagatag agttaaaaac ttcccttcag attcaagtca
    13021 acaaattata aaaaatgcca tatcgtcacc caataatttc tttaatagtt taggttatga
    13081 atcattatcc tgttccgcta ttgatgaaac ggatggtgca agatttactt taaataatgg
    13141 tgatattata catctccgtc cttccggtaa tgctccagaa ctccgttgtt atgctgaggc
    13201 cagtgatgaa aatcaggcta agcaatatgt tacgaatgtg ctgggaaata ttacctcttt
    13261 gatttcttga tgttataggt ttatctacgc ttatatgtgt gcgtaggttt gattacacgt
    13321 agatgccggt atacagaatt gaagaacggt atttgttgca ttaatgaaat tcagcactac
    13381 acacattcgt gcaacttgag ataacatctc aatcatattc aagtcgcgca tacatcgcgg
    13441 tgaacacccc ctgacaggag taaacaatgt caaagcaaca gatcggcgtc gtcggtatgg
    13501 cagtgatggg gcgcaacctt gcgctcaaca tcgaaagccg tggttatacc gtctctattt
    13561 tcaaccgttc ccgtgagaaa acggaagaag tgattgccga aaatccgggc aagaaactgg
    13621 ttccttacta tacggtgaaa gagtttgttg aatctctgga aacgcgtcgt cgcatcctgt
    13681 taatggtgaa agcaagtgca ggcacggatg ctgctattga ttccctcaaa ccatatctcg
    13741 ataaaggcga catcatcatt gatggtggta acaccttctt ccaggacacc attcgtcgta
    13801 accgtgagct ttctgccgaa ggctttaact tcattggtac cggtgtctcc ggtggtgaag
    13861 aaggcgcgct gaaaggtcct tccattatgc ctggtgggca gaaagaagcc tatgaactgg
    13921 ttgcgccgat cctgaccaaa atcgccgcag tggctgaaga cggcgagcca tgcgttacct
    13981 atattggtgc cgatggtgca ggtcactatg tgaagatggt tcacaacggt attgaatacg
    14041 gcgatatgca gctgattgct gaagcctatt ctctgcttaa aggtggcctg aatctcacca
    14101 acgaagaact agcgcagatc tttaccgagt ggaataacgg tgaacttagc agctacctga
    14161 tcgacatcac caaagatatc ttcaccaaaa aagatgaaga gggtaactac ctggttgatg
    14221 tgattctgga tgaagcagca aacaaaggta cgggcaaatg gaccagccag agtgcgctcg
    14281 atctcggcga accgctgtcg ctgattaccg agtctgtgtt tgcacgttat atctcttctc
    14341 tgaaagatca gcgcgttgcc gcttctaaag ttctctctgg cccgcaagcg cagccagcag
    14401 gcgataaggc tgagttcatc gaaaaagttc gtcgtgcgct gtatctgggc aaaatcgttt
    14461 cttacgctca gggcttctct caactgcgtg ctgcgtctga agagtacaac tgggatctga
    14521 actacggcga aatcgcgaag attttccgtg ctggctgcat catccgtgcg cagttcctgc
    14581 agaaaatcac cgatgcctat gccgaaaatc cgcagatcgc taacctgctg ctggctccgt
    14641 acttcaagca aattgccgat gactaccagc aggcgctgcg cgatgtcgtc gcttatgcgg
    14701 tacagaacgg tatacccggt tccgaccttt gctgctg
//
