LOCUS       EU296414                8615 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Shigella dysenteriae type 6 O-antigen gene cluster, complete
            sequence.
ACCESSION   EU296414
VERSION     EU296414.1
KEYWORDS    .
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8615)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 8615)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..8615
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:622"
                     /note="type: 6"
     gene            340..1614
                     /gene="wzx"
     CDS             340..1614
                     /gene="wzx"
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ACD37093.1"
                     /translation="MLDILRIQEKYFMKFTLKKTNIQNRNVVIYLISDLLAKALPFLF
                     IPVLTKYLTPEQYGNIALFNIGVETFLIIIIMGGNSYYKIEYPKFKDPILSLYIIIYN
                     VTLLFLVFFILSIVICFFYERIYIIDVLPLILLCAYFQSLFYLYISYYQCNEKALVVG
                     MVNLFFSLSSSLLMILFLVEFKQAESSRYWSLLGGLIASVLLLGSILANKSNRKYKLV
                     NKIDFELIKFGVGVFPHAVSWWARSGMERLLIGWFLSVSTLGIYSLAMQLTSIMPLFC
                     NAINQALMPRIVRCLNDNKIEETKKILVKSSLIVAIACVFSSVVMPLSLGYVLNDRYH
                     EALEYLPYMILSFVFQGVIIIYTNVLYFYKQVKYLSVATFGTSCVHLLLSMLLIKINL
                     NVYSVIISSVITFLFASIVLVRKAYQIMSERY"
     misc_feature    340..8512
                     /note="O-antigen gene cluster"
     gene            1615..2415
                     /gene="wffF"
     CDS             1615..2415
                     /gene="wffF"
                     /note="glycerol-3-phosphate cytidyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WffF"
                     /protein_id="ACD37094.1"
                     /translation="MILTKIKNRLIIFVSKLLYQSRAYYYRCKNRKKKKIFIYTDSRG
                     YEVSNLWNKKNPFSSYVGDLVKEYNVEYHICEYSSTTIIDFLYEYYGEGTRGKKYDYV
                     IAHVGLVDFSPRPRSMANDIIKSKSHKIKFFKWDLRMFDKYLEREISSEIYNGELLSN
                     LYDCEFLKNEIIPLLQNIPNLIYIGCNPIISNWRGNYWQDRPKDINRILMAYNSVMLT
                     ELKEKKVVDIKDWNECDIRNYTVDNIHLNREGYNKITLNLKQILTEIK"
     gene            2415..3614
                     /gene="wffG"
     CDS             2415..3614
                     /gene="wffG"
                     /note="glycerophosphotransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WffG"
                     /protein_id="ACD37095.1"
                     /translation="MKFIFTRLVIFLLSVFVPTKKGLFIFGSWFGKKYGDNTRALFEH
                     LSNIQPENVYWYTDNEKIASKIIASGNKCISGVNMKNIFLHLRAEAVFCNCSANSDLL
                     GGYINKRTKVFNLWHGTPMKKIGKDAVKSGLSHTRLGMSKPNLTLSLIKKILPSTFYN
                     FLKLDTYYLASSPEVSKILQGAMGVNEEKIIICGYPKLDKIFIESGYAEPYKILYAPT
                     YRGEYNSELDILTMFGFNIELADKVLKENKATLTIRLHPANKLPVAVINRINNTNTIS
                     IDNEDDIYESLGKYSLVITDYSSVYFDALAVGINVLIAPFGYKDYLENDRDLYFSLSE
                     LYPERMPIDWKDFFEHFSYFASNKREQFSSIRNKFYSFPYAPCSDKLLSLVYLKLGRE
                     YVNKKNY"
     gene            3592..5634
                     /gene="wffH"
     CDS             3592..5634
                     /gene="wffH"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37096.1"
                     /translation="MLTKRTTKNTSFLYSSLFCIYFSVSFSPVNPTYILGCGLILILF
                     MQVLLRMRIQKFTLITIVFAMMFLLEQLIGINIVSFYPEGPVYLTPLLFSYFILISAC
                     VIEGFQGLKSDSRLIIYKRVYNYAIIFLVIELFTRVLNYNKENNGFYSLKYSVLYFDS
                     NFTGLVILSLCSYLLYLKYVKGNDLKIQRRILYFLLLATFSRGAIAALILTRIGLGSK
                     KRIRGKAFLIIAVAVVIFSILTIEYIEQSQSYSSIDGSFNSKFYIISQALSFYESLPI
                     LVHWFGIGMGNTSQFIGIFAHNIFVTMILEMGIVGTVLFLLYVFYTLKLSNGYALFIW
                     VPIFVGGISLFSAFSPFAFIICALICCENMVDKNMSFRDNPKVSVLIPAYNVAEWIEE
                     SLLSILGQSYENIEVIVVDDCSTDGTYEIIRRVAENEPRMKVLRNERNMKIVDTLNYG
                     LTHCTGDFVLRHDGDDVSEIKRIEVQLKHLLENELDLVGTQMLPIDRSGRVIGAQSRL
                     PLSHELIEKIANFSSPLTHIWICKKEIYDKLGGYRKVPYAEDFDFLLRAIDSGFKCGN
                     TSVALTKIRHRHGNTSDIASLSQRKGYFYALELHKQRKAQNYDSYNDVDAAKLIKHNK
                     LVNYFHSLSTKCLHKAFQSNNKLEMFFYTLLSAVMSFYNAHYIYQRIKVKMLLSNQ"
     gene            5705..6748
                     /gene="wffI"
     CDS             5705..6748
                     /gene="wffI"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37097.1"
                     /translation="MRILILIEDITLGGGTERVALTLATNLNADGINCDIFSLSKSNT
                     DTFYPSENINICYAKSKVGVFAKIEAIKYAKKNNMKLLVFSMGRLSVEIALLSRLFLF
                     RHFGCYEHISFSSFSGFIQKIKLFAYRLSHGVIFLTEHDRDIIRHKLSHVPVTSIENI
                     SPFRAIKKSNVGARKNIVLAVGRLTNQKNFSRLINLWSMVNRPDWSLIIAGDGPDRDF
                     LDKQINHLCLKNVEIVGAVKEISNMYLASKILVLTSRYEGFPMVMVEAQCFGLPAVSF
                     DCKTGPSEIIINESTGYIIPYHDDSLFIEKLQRLIDHPKELETFSYNSIKNAQRFTYE
                     NTKCKWLEILEKI"
     gene            6748..7500
                     /gene="wffJ"
     CDS             6748..7500
                     /gene="wffJ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37098.1"
                     /translation="MNENEHEIVSIIMPAYNAEATIKSSVYSVIGQTFSKFKLYIIND
                     ASTDCTEEIIRSFEDSRIVYIKNDYNIGVAESRNKGIKKCKGKYIAFIDSDDLWEPNK
                     LELQYIHLNAGMDIVCSNYVTFSENPMRIKNKRIAPQYIRYNDMLKSNFIGNLTGIYN
                     SDKLGKIYQKKIGHEDYVMWLELIKRAKCCYCVQEYLAKYRESNKSLSGNKLRAIVWQ
                     WSIYRKELNMGLISTIYYFSSYIYNGILKRNN"
     gene            7514..8512
                     /gene="gne"
     CDS             7514..8512
                     /gene="gne"
                     /note="GlcNAc-4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gne"
                     /protein_id="ACD37099.1"
                     /translation="MAILVTGGTGYIGSHTVTELLKEGYEVIVIDNLINSAYDVVDRI
                     KKLSKRDFVFYKGDITDESLLFKIFTENNITDVIHFAGLKSVGESFSKPVEYYNINVT
                     GTLKLVNAMLLAGVQHIIFSSSATVYGIPEKIPLTEQCSVGATTNPYGTSKFMAERIL
                     QDIANANKKFHVTLLRYFNPVGAHPSGLIGEQPQGIPNNLVPFLTMVASGKLDMLSVF
                     GNDYPTKDGSGVRDYIHVMDLAEGHIAALKHNPTHSNFHVYNLGTGQGYSVLELIKIF
                     EEVTGIAVKYNIADRRPGDIAECWADPSLAEEELRWRAHRTIEQMMVDAWSWQLKN"
BASE COUNT     2946 a   1077 c   1583 g   3009 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg ttaaatttga tgctaagcgg taaaaagaat
       61 atagaatgta atattttatt atttataatc tttaatctgg tgtttcatca ttgtttagta
      121 tgctcataaa atggtggcaa aatcattttt tttaaattta tagtaaaata cgaattcaga
      181 cataatgcaa ttatctcaat tgagagagat actaatgtta atatttattg taataaatat
      241 tggaactata tagataagat aataacttgt ggataagtag cataaattgc tgtgaaaatg
      301 gctgagcgtg acctgttaac atagaatggt catttattaa tgcttgatat attaagaata
      361 caagaaaaat atttcatgaa atttacgtta aaaaaaacaa atattcaaaa tagaaatgtt
      421 gttatttact taataagtga tctacttgca aaggccttgc catttttatt tataccagta
      481 ttgacaaagt atctcacgcc agagcaatat ggcaatattg cattattcaa tataggtgtg
      541 gaaacctttc ttataataat tattatggga gggaactcct attataagat tgaatatcca
      601 aaatttaaag acccaattct ttctttgtat ataattatat ataatgttac tttgttattt
      661 ttggtatttt ttattttatc gatagtaata tgtttttttt atgaaaggat ttatattata
      721 gatgtgctgc ctttgatatt attatgcgca tattttcagt cgttatttta tttatatata
      781 agctattatc aatgtaatga aaaagccttg gttgtaggaa tggtgaattt atttttctca
      841 ctatcaagtt cattgttgat gattctattt cttgtcgaat ttaaacaggc tgaaagttca
      901 cgatattggt ctttgttagg agggctaatt gcatccgtat tattgttagg tagcatttta
      961 gctaacaaaa gcaatagaaa atataaatta gtcaataaga ttgattttga gttaattaag
     1021 tttggagtcg gagtgtttcc gcatgctgta agctggtggg ccagaagtgg aatggagcgt
     1081 ctgttgattg gttggttctt atccgtatca acattgggta tttattcgct agctatgcag
     1141 ttgacatcca taatgccatt attttgtaat gcaatcaacc aagcattgat gccaagaatt
     1201 gtaaggtgtt taaatgataa taaaattgaa gaaacaaaga agatattagt taagtcatca
     1261 ctaattgtag caatagcatg tgtattttca tcagtagtta tgcctctgtc ccttggatat
     1321 gttcttaacg atagatacca tgaggcccta gagtatttgc cttatatgat tctatcattt
     1381 gtatttcaag gtgtaattat catttatact aacgttttat atttttataa acaagtaaaa
     1441 tatctttctg ttgctacttt tggaacgtca tgcgttcatt tgttgctatc tatgttgttg
     1501 ataaaaatta atttaaacgt atattctgta ataatatcat ctgtaatcac gtttttattc
     1561 gcatctattg tacttgttag aaaagcatat cagataatga gtgagaggta ttaagtgatc
     1621 ttaactaaga taaaaaatag actaataata tttgtatcta aattattgta tcaatcccgt
     1681 gcctattact atagatgtaa aaacaggaaa aagaaaaaga tatttattta tacggattcg
     1741 agaggttatg aggtaagcaa cttatggaat aagaaaaatc ccttttcatc atacgtaggg
     1801 gatttggtaa aagaatataa tgttgaatat catatatgtg agtactcgag tacaacaata
     1861 attgattttt tatatgaata ttatggggag ggtactagag ggaaaaaata tgattacgta
     1921 attgcgcatg taggattagt tgatttttct ccaaggccaa gaagtatggc taacgatata
     1981 attaagagta agtctcataa aattaaattt tttaaatggg atttacggat gtttgataaa
     2041 tatttggaaa gagaaatttc atcagaaatt tataatggcg aattattatc taatttgtat
     2101 gattgtgagt tcttaaagaa tgaaatcatt ccattattac aaaatatacc caacttaatc
     2161 tacattgggt gtaaccctat tatatccaat tggcgaggta attattggca agaccgacca
     2221 aaagatatca acagaatact tatggcatac aatagcgtta tgttaactga attaaaagaa
     2281 aagaaagttg ttgatattaa agattggaat gaatgcgata taagaaatta tacggttgat
     2341 aatatacatt taaatcgaga aggttataat aaaataacat tgaatctaaa acaaatatta
     2401 acggaaatta agtgatgaag tttatattta caagattagt aatattttta ctttctgtat
     2461 tcgttccaac caaaaaaggt ctttttattt ttggtagttg gtttggtaaa aaatatggtg
     2521 acaatacacg agctttgttt gaacatttat caaatataca accagaaaat gtatattggt
     2581 atacagataa tgaaaagatt gcctctaaaa taatagcctc tgggaataaa tgtatttctg
     2641 gagttaatat gaaaaatatt tttttgcatt tgcgcgcaga agctgtgttt tgtaattgtt
     2701 ctgcaaattc tgatttatta ggggggtata taaataagcg aacaaaggta tttaatcttt
     2761 ggcatggcac acctatgaaa aaaataggca aagatgcagt aaaatccgga ttgagtcata
     2821 ctcggctagg gatgtcgaaa ccaaacttaa cattgagtct aataaaaaaa atattaccaa
     2881 gcacatttta taatttttta aagttagaca cttattattt ggctagttct cctgaggttt
     2941 ctaaaatatt acagggagca atgggggtga acgaagaaaa aataattatt tgtggttacc
     3001 ctaaattaga taaaatcttt atagaaagtg gatatgctga accttacaaa attttgtatg
     3061 cccctaccta tagaggtgaa tataatagtg agttggatat attgacaatg ttcggtttta
     3121 atattgaact tgcggataaa gttttaaaag agaataaagc tactttgact attagacttc
     3181 accctgctaa taaattgcca gtagctgtaa ttaatagaat aaacaataca aatacaatat
     3241 caatagataa tgaagatgat atttatgaaa gtttaggtaa atattcactg gtaataactg
     3301 attattcaag tgtttatttc gatgcattag cagtgggaat taatgtactg attgctccat
     3361 ttggttataa ggattattta gaaaatgacc gagatttgta ttttagccta agtgagttat
     3421 atccagaacg aatgcctatt gattggaagg atttttttga acattttagc tattttgcgt
     3481 cgaataaaag agagcaattt tcatccatca gaaacaagtt ttacagcttt ccatatgcac
     3541 cttgttcgga taagttatta agtttggtat atttaaaatt aggtagagaa tatgttaaca
     3601 aaaagaacta ctaaaaatac tagctttctt tattcctctc ttttttgcat ttattttagt
     3661 gtaagtttta gcccggtcaa tccaacttat atattaggat gtggattaat tcttatactt
     3721 tttatgcaag ttttgcttcg tatgaggata caaaaattta cactgataac aattgttttc
     3781 gctatgatgt ttttattgga acaattgata gggattaaca ttgtctcatt ttatcctgag
     3841 ggtcctgtat atttaacccc tttacttttt tcctatttta ttctgatttc tgcttgcgtt
     3901 attgaaggtt ttcaggggtt aaaaagtgat tctagattga taatatataa aagagtttat
     3961 aattacgcaa tcatattttt agttatagaa ttattcacta gagttttgaa ttataacaag
     4021 gaaaacaatg ggttttattc tttaaaatat agtgttcttt attttgattc taattttact
     4081 gggttggtta ttctttcgtt atgttcatat ttgctttatc taaaatatgt taaaggtaat
     4141 gatctaaaaa tccagcgtag aattctatat tttttgttat tagcaacgtt ttctcgtggt
     4201 gcaatagctg cattgatctt aactcgtatt ggcttgggta gtaaaaaaag aattagaggt
     4261 aaagcatttt taataatagc agttgctgtt gtcattttta gtattctaac gatagaatat
     4321 attgagcagt cacaaagtta ttcatcgatt gatgggagtt ttaatagtaa gttttacatt
     4381 attagccaag cgctatcatt ttatgaaagt cttccaattc ttgtgcattg gtttggcatt
     4441 ggaatgggaa atacttctca atttataggt atatttgcac acaatatatt tgtaacaatg
     4501 atcttagaaa tggggatagt tggtactgtt ctttttttat tatatgtatt ttatacgtta
     4561 aagttatcaa atggttatgc attatttata tgggttccta tatttgttgg cggaatttca
     4621 ctttttagtg ctttttcacc atttgctttc atcatctgtg ctttaatttg ttgtgaaaat
     4681 atggtggata aaaatatgag ttttagagat aatccgaaag tcagtgtctt aatccctgcg
     4741 tacaatgtag ccgaatggat tgaagaatcg ctattaagta tattagggca atcgtatgaa
     4801 aatatagaag ttattgtagt ggatgattgc tcaactgatg gaacgtatga aattatacgt
     4861 cgggttgccg agaatgaacc gcgaatgaag gttttaagaa atgaacgtaa tatgaaaata
     4921 gtagatacat taaattatgg cttaacccat tgcacaggtg attttgtttt gaggcatgac
     4981 ggtgatgatg tatcggaaat taaacggatt gaggtgcaac ttaaacacct gttagaaaat
     5041 gagctagact tagttggaac tcaaatgcta ccaattgata ggtctggtcg agttattggt
     5101 gctcaaagta gattaccttt atctcatgag ttgatcgaaa agattgcaaa ctttagctca
     5161 ccattgaccc acatatggat atgtaaaaaa gagatatatg ataaactggg tggctatcgt
     5221 aaggtaccat atgcagaaga ttttgatttt ctgttaaggg cgattgattc aggttttaaa
     5281 tgtggcaaca catctgttgc gttaacaaaa attagacatc gtcatggaaa tacgagtgat
     5341 attgctagtc tttcccagcg taaaggatat ttttatgcat tagaattgca taaacaaagg
     5401 aaagcgcaaa actatgatag ttataatgat gttgacgcag caaaattaat aaaacacaat
     5461 aaactagtaa actatttcca ctcattatct acaaagtgtt tgcataaagc atttcagagc
     5521 aataataaat tagaaatgtt tttttataca ttgctatcgg cagtaatgtc attttataat
     5581 gcgcattata tatatcaaag aatcaaagtt aaaatgctat taagcaacca gtagtatttt
     5641 tatattggaa ggacttgcta agattattta ttttctacaa tgagaggaat tgcaaatata
     5701 aattatgagg atactaattt taatagaaga tataacgtta ggtggaggca ctgaacgcgt
     5761 agccctcaca ttagcgacaa atttaaatgc tgacggaatt aattgtgata tattttcact
     5821 ttcaaaatca aatacggata cattttatcc gtcagagaat attaatatat gttatgctaa
     5881 gtcgaaggtt ggtgtttttg caaaaattga agcaattaaa tatgcaaaaa aaaacaatat
     5941 gaaactgtta gttttttcta tggggaggtt gtctgttgaa atcgctttac ttagcagact
     6001 cttcttattt aggcattttg gctgctacga gcatatttct ttctcatcat tttctggttt
     6061 tatccaaaaa atcaaattat tcgcataccg tttatcccat ggtgttattt ttttgactga
     6121 gcatgatcgt gatataattc gacataagtt gtctcatgtc ccagttacct ctattgaaaa
     6181 cattagtcct tttcgagcaa taaaaaaaag taatgtaggt gctaggaaga atattgtttt
     6241 agctgtggga cgattgacta atcagaaaaa tttttctcgt ttaattaatt tatggagcat
     6301 ggtaaacaga cctgactgga gtttgattat tgctggagat ggtccagaca gagatttcct
     6361 tgataaacaa attaatcatt tatgtttgaa aaacgtagag attgttggcg ctgtaaaaga
     6421 aattagtaat atgtatttgg cttctaaaat actagtgttg acatctcgct atgaaggttt
     6481 tcctatggta atggttgaag cccagtgttt tggactacct gcagtttcat ttgattgtaa
     6541 aacggggcct tcagaaatca taatcaatga atctacggga tatataatac cctaccatga
     6601 tgactcgctt ttcattgaaa agttgcaaag gttgatagat catccgaaag aattagaaac
     6661 gtttagctat aattcaatta agaatgcaca acgttttaca tatgaaaaca ctaagtgcaa
     6721 gtggttggaa atactagaga aaatataatg aatgaaaatg aacatgaaat tgtatcaatt
     6781 attatgcctg cttataatgc tgaagcaacg attaaaagta gtgtttattc agttattgga
     6841 cagacctttt caaaatttaa gttgtatatt attaatgatg cgtcaacgga ttgtacagaa
     6901 gaaattataa gaagttttga agattcaagg attgtttata tcaaaaatga ttataatata
     6961 ggtgtggccg aatcaagaaa taaaggaatt aaaaaatgta agggcaaata tattgctttc
     7021 attgacagcg atgacctgtg ggaacctaac aaattggaat tacaatatat tcatttgaac
     7081 gcaggtatgg acattgtttg ttcaaattat gtaactttct ctgaaaaccc aatgcgtatt
     7141 aaaaataaaa gaatagcacc acagtacata agatataatg atatgctgaa gtctaacttt
     7201 atcggtaatc ttacagggat ctataactcg gataagttag ggaagatata tcaaaaaaaa
     7261 ataggccacg aagattatgt tatgtggctt gaattaataa agcgtgcaaa atgttgttat
     7321 tgcgttcaag aatacctggc taaatatcgc gagtcaaata aatccctttc gggaaataag
     7381 ttaagagcaa ttgtatggca atggagtatt tatagaaaag aattaaatat gggattaatc
     7441 agtacaatat attacttttc aagttatatt tataatggga tcttaaaaag aaacaactaa
     7501 actggggagg ggtatggcta ttttagttac aggcggaaca ggttatattg gatctcatac
     7561 ggttacagag ttacttaagg agggatatga agttattgta attgataatc ttattaactc
     7621 tgcatatgat gtggtcgata ggataaagaa actttcaaaa cgtgatttcg ttttttataa
     7681 aggtgatatt actgatgaaa gtttattgtt taaaatattt actgaaaata acatcactga
     7741 tgtgattcat tttgctggtt taaaatctgt aggcgaatct ttttctaaac cagttgaata
     7801 ttacaatata aatgttacag gcacgctaaa attagttaat gcaatgcttc ttgctggtgt
     7861 gcaacatatt atttttagtt cctcagcaac tgtttatgga atacctgaaa aaataccatt
     7921 aacagaacaa tgttctgtag gtgcaacaac caacccatat ggaacttcaa agtttatggc
     7981 tgagcgcata ctacaagata ttgctaatgc aaataaaaaa ttccatgtca ccttattaag
     8041 atatttcaac ccagtgggtg ctcatccatc aggattaatt ggtgaacagc ctcaaggaat
     8101 tccaaataat ttggttccgt ttttaactat ggttgcaagt ggtaaacttg atatgttatc
     8161 tgtttttggc aatgattacc ccacaaaaga tggaagcgga gttcgtgatt atatccacgt
     8221 aatggattta gctgaaggcc atatagcagc attaaaacat aatccgacgc attctaattt
     8281 ccatgtatac aatttaggta ctggtcaagg atactctgta ttagagttga ttaagatatt
     8341 tgaagaagtt acgggaatag cagtgaaata taatatagct gatagaaggc ccggtgatat
     8401 agcagaatgt tgggctgatc cttctttagc agaagaggag ctaagatgga gggctcatag
     8461 gactatcgag cagatgatgg tagatgcatg gagttggcaa cttaaaaact gatatagcta
     8521 agtagtaaat ttattttgaa aggctatgaa gattcatttg aactaagtta attttatttt
     8581 atccatagtg cacgccttac tgtgcgtatg atagg
//
