LOCUS       EU294165                7919 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Shigella flexneri 6 O antigen gene cluster, complete sequence.
ACCESSION   EU294165
VERSION     EU294165.1
KEYWORDS    .
SOURCE      Shigella flexneri 6
  ORGANISM  Shigella flexneri 6
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 7919)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..7919
                     /organism="Shigella flexneri 6"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:424719"
                     /note="type: 6"
     gene            107..1192
                     /gene="rmlB"
     CDS             107..1192
                     /gene="rmlB"
                     /note="dTDP-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ACA24767.1"
                     /translation="MKILVTGGAGFIGFAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKTRFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTLEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     misc_feature    107..7777
                     /note="O antigen gene cluster"
     gene            1192..2091
                     /gene="rmlD"
     CDS             1192..2091
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ACA24768.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKRIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKEANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAAKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELITTTAI"
     gene            2149..3027
                     /gene="rmlA"
     CDS             2149..3027
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ACA24769.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKALAEPL
                     KKNAYGQYLLKMIKGY"
     gene            3032..3589
                     /gene="rmlC"
     CDS             3032..3589
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ACA24770.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYHPDSDRGIVWNDPILSIKWPTI
                     EHNNYILSIKDARAKELHNMKELFL"
     gene            3586..4818
                     /gene="wzx"
     CDS             3586..4818
                     /gene="wzx"
                     /note="O antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ACA24771.1"
                     /translation="MSIVKNTLWNISGYIIPSLIAIPALGILSRILGTEQFGLFTLAI
                     ALVGYASIFDAGLTRAVIREVSIYKNVHKELRAIISTSTVILTILGLIGGSVLFLSSN
                     VIVKLLNINANHVVESVKAIYIISATIPLYLLNQVWLGIFEGMEKFRKVNLIKSINNS
                     FVAGLPVIFCFFHGGLLSAIYGLVMARVLSLIVTFIFSRKLIISSGLSVKIVTVKRLI
                     GFGSWITVSNIISPIMTYMDRFILSHIVGADKVSFYTAPSEGIQRLTILPSALSRAIF
                     PRLSSELQSVKQTKILSYFIMVIGILPIVMLIIILSDFIMSAWMGPTYHGTPGIVLKI
                     LAIGFFFNCIAQIPFVSVQASGRSKITAIIHLLEVIPYLCILYIFIYHWGIVGAAIAW
                     SVRTSLDFLILLLIDTKY"
     gene            4874..6061
                     /gene="wzy"
     CDS             4874..6061
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ACA24772.1"
                     /translation="MTSDFYNSKDKSLSVLLFFGFIFFLTRSFPFIQYSWIMEGFLCL
                     CIMSFTKKIANGIYHYPVILIFLLALFINFIYSYIKGNDIAIIIRFYIIILFILCAYF
                     CSYGTISIVKIFLYLMVLQAVIISIISIYMTKTYGIGDYSALRHYFLENDYGDVYTYG
                     SGFYRVQIKGNALIPFAFMLHIVIKDYFYYRFKNTITVILAIGTIVAGNFAYFVSICL
                     FFMYIILCSKSNSRYAKLRKIIFGVFLTVILPFFITYSIELIIMKSNGADSSLGVRWD
                     QFTVLINDLTESVSNFVIGSGLGNVIKIQTPIRDYSAYIYYELQSVYFLNQLGVILFT
                     LFLLINLLLTIKIIKYSELCVLYFLYVSYAITNPYILDSNHVAVIIVLVTLSNVLKKM
                     KAK"
     gene            6078..6947
                     /gene="wfbY"
     CDS             6078..6947
                     /gene="wfbY"
                     /note="rhamnosyltransferas"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24773.1"
                     /translation="MDTVYAVLVAYNPEHNDLKNAVELLLRQVTKVVVCNNSTNGYKY
                     AENSSGDVKIFNFNDNLGIAEAQSIGMKWAFENGADFILQMDQDSIPDPKMVEQLLTC
                     YKKLLKQNVNVGLVGSQDFDKVTGELNKARVKKGKPLTEVYYEVDSTLSSGSLIPKNS
                     WLIVGGMKDELFIDAVDHEYCWRLRAAGFKVIRNKNALLAHRLGDGRFKILNILSVGL
                     PSPFRHYYATRNIFLLLNKNYVPIYWKISSLVKLIGKVFLYPIFLPNGNKRLYFFLKG
                     INDGLMGRSGKMK"
     gene            6944..7777
                     /gene="wfbZ"
     CDS             6944..7777
                     /gene="wfbZ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24774.1"
                     /translation="MNHRLEKFSVLISIYKNDLPQFFEVALRSIFHDQTLKPDQIVIV
                     ADGELHQTHIDIINSFIDDVGNKIVTFVPLPRNVGLANALNEGLKACRNELVARMDAD
                     DISLPHRFEKQISFMINNSEIDVCGSFIDEIETVTEEFISTRKVPLEHREIVKFARKR
                     SAVSHPSVIFRKNTVLAVGGYPPFRKSQDFALWSLLIVHNARFANLPDILLKMRTGRN
                     LMARRGLSYLLYEYKVLYYQYKIGFIRKNELISNAMLRTFFRIMPSKLKELMYSIVRN
                     R"
BASE COUNT     2406 a   1233 c   1649 g   2631 t
ORIGIN
        1 ttggtagctg taagccaagg gcggtagcgt gcattaatac ttctattaat caaactgaga
       61 gccgcttatt tcacagcatg ctctgaagca atatggaata aattaggtga aaatacttgt
      121 tactggtggc gcaggattta ttggttttgc tgtagttcgt cacattataa ataatacgca
      181 ggatagtgtt gttaatgtcg ataaattaac gtacgccgga aacctggaat cacttgctga
      241 tgtttctgat tctgaacgct atgtttttga acatgcggat atttgcgatg cagctgcaat
      301 ggcacggatt tttgctcagc atcagccaga tgcagtgatg cacctggctg ctgaaagcca
      361 tgttgaccgt tcaattacag gtcctgcggc atttattgaa accaatattg ttggtacata
      421 tgtccttttg gaagccgctc gcaattattg gtctgctctt gatagcgaca agaaaactag
      481 attccgtttt catcatattt ctactgacga agtctatggt gatttgcctc atcctgacga
      541 ggtaaataat acagaagaat tacccttatt tacagagaca acagcttacg cgccaagcag
      601 cccttattcc gcttcaaaag catccagcga tcatttagtc cgcgcgtgga aacgtaccta
      661 tggtttacca accattgtga ctaattgctc taataattat ggtccttatc atttcccgga
      721 aaaattgatt ccattggtta ttctgaatgc tctggaaggt aaggcattac ctatttatgg
      781 caaaggggat caaattcgtg actggctgta tgttgaagat catgcgcgtg cgttatatac
      841 cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacaca acgaaaagaa
      901 aaacatcgat gtagtgctca ctatttgtga tttgctggat gagattgtac cgaaagagaa
      961 atcttaccgc gagcaaatta cttatgttgc cgatcgcccg ggacacgatc gccgttatgc
     1021 gattgatgca gagaagatta gccgcgaatt gggctggaaa ccgcaggaaa cgtttgagag
     1081 cgggattcgg aagacattgg aatggtacct gtccaataca aaatgggttg ataatgtgaa
     1141 aagtggtgct tatcaatcgt ggattgaaca gaactatgag ggccgccagt aatgaatatc
     1201 ctccttttcg gcaaaacagg gcaggtaggt tgggaactac agcgtgctct ggcacctttg
     1261 ggtaatttga ttgctcttga tgttcactcc actgattatt gtggtgattt tagtaatcct
     1321 gaaggtgtag ctgaaacagt caaaagaatt cgacctgatg ttattgttaa tgctgcggct
     1381 cacaccgcag tagataaggc tgagtcagaa cccgaatttg cacaattact caatgcgact
     1441 agtgttgaat caattgcaaa agaggctaat gaagttgggg cttgggtaat tcattactca
     1501 actgactacg tattccctgg aaatggcgac acgccatggc tggagacgga tgcaaccgca
     1561 ccgctaaatg tttacggtga aaccaagtta gccggagaaa aagcgttaca ggaacattgc
     1621 gcgaagcatc ttattttccg taccagctgg gtatacgcag ctaaaggaaa taacttcgcc
     1681 aaaacgatgt tgcgtctggc aaaagagcgc gaagaactgg ctgtgataaa tgatcaattt
     1741 ggtgcgccaa caggtgctga gctgctggct gattgtacgg cacatgctat tcgtgtggca
     1801 ctgaataaac cggaagtcgc aggtttgtac catctggtag ccagtggtac cacaacctgg
     1861 cacgattatg ctgcgctggt ttttgaagag gcgcgcaaag caggtattcc ccttgcactc
     1921 aacaagctca acgcagtacc aacaacagcc tatcctacac cagctcgtcg tccacataac
     1981 tctcgcctta atacagaaaa atttcagcag aactttgcgc ttgtcttgcc tgactggcag
     2041 gttggtgtga aacgaatgct caacgaatta attacgacta cagcaattta atagtttttg
     2101 catcttgttc gtgatggtgg agcaagatga attaaaagga atgatgaaat gaaaacgcgt
     2161 aaaggtatta ttttagcggg tggttctggt acacgtcttt atcctgtgac tatggctgtc
     2221 agtaaacagc tattacctat ttatgataag ccgatgatct attacccgct ctctacactg
     2281 atgttggcgg gtattcgcga tattctgatt attagtacgc cacaggatac tcctcgtttt
     2341 caacaactgc taggtgacgg tagccagtgg gggctaaatc ttcagtacaa agtgcaaccg
     2401 actccagatg ggcttgcgca ggcgtttatt atcggtgaag agtttatcgg tggtgatgat
     2461 tgtgctttgg ttcttggtga taatatcttc tacggtcatg atctgccgaa gttaatggat
     2521 gtcgctgtta acaaagaaag tggtgcaacg gtatttgcct atcacgttaa tgatcctgaa
     2581 cgctacggcg tcgttgagtt tgataaaaac ggtacggcaa taagcctgga agaaaaaccg
     2641 ctacaaccaa aaagtaatta tgcggtaacc gggctttatt tctatgataa cgacgttgtc
     2701 gaaatggcga aaaaccttaa gccttctgcc cgtggtgaac tggaaattac cgatattaac
     2761 cgtatttata tggaacaggg gcgtttatcc gttgccatga tggggcgtgg ttatgcatgg
     2821 ctggatacgg ggacacatca gagtcttatt gaagcaagca acttcattgc caccattgaa
     2881 gagcgccagg gactaaaggt ttcctgccca gaagaaattg cttaccgtaa agggtttatt
     2941 gatgctgaac aggtgaaagc attagcggag ccgctgaaaa aaaatgctta tggacagtat
     3001 ctgctgaaaa tgattaaagg ttattaataa aatgaacgta attaaaacag aaattcctga
     3061 tgtgttaatt ttcgagccga aagtttttgg tgatgagcgt ggtttcttta tggaaagctt
     3121 taatcagaaa gttttcgaag aagctgtagg acgtaaggtt gaatttgttc aggataacca
     3181 ttcgaagtct agtaaaggtg ttttacgcgg gctgcattat cagttagaac cttatgcgca
     3241 agggaaactg gtacgttgcg ttgttggtga ggtttttgat gtagctgttg atattcgtaa
     3301 atcgtcgcct acctttggta aatgggttgg ggtgaattta tctgctgaga ataagcggca
     3361 attgtggatc cctgagggat ttgcacatgg ttttttggtg ctgagcgaga ctgcggaatt
     3421 tttatataaa acgacgaact attatcatcc tgatagtgat agagggattg tatggaatga
     3481 tcctattctg agcataaaat ggccgacgat agaacataat aattatattt tatcgattaa
     3541 agatgcaagg gctaaagaat tgcataacat gaaggaatta tttttgtgag tattgtaaag
     3601 aatactttat ggaatataag tgggtatatt ataccatcat taatagcaat tcctgcgtta
     3661 ggtatactgt ctagaattct agggaccgag caatttggcc tttttacgtt agctattgcc
     3721 ttagttggat atgcaagtat ttttgatgct ggattgacca gagctgttat aagagaagta
     3781 tcaatatata aaaatgttca taaagaatta agagcgatca tttcaacttc aacggtaatt
     3841 ctaactatat tgggcttgat tggcggtagt gtactatttt tgagtagcaa tgtaattgtt
     3901 aaattattaa acattaacgc gaatcatgtt gtagaatctg tcaaagcaat atatattatt
     3961 tcagctacca tacccttata cttgttaaac caagtctggt tggggatttt tgaggggatg
     4021 gaaaagttca gaaaagtaaa tttaataaaa tcaattaaca actcttttgt ggctggatta
     4081 ccagtgattt tctgtttttt tcatggagga ttactaagtg ctatatatgg tttagttatg
     4141 gcaagagtct tatcacttat agtgaccttt atatttagtc gaaaactaat aatatcatct
     4201 gggctgtctg taaaaattgt aacagttaaa agattaatcg gctttggaag ctggataaca
     4261 gttagcaata ttattagccc tattatgaca tatatggatc gttttattct ttcacacatt
     4321 gtgggggctg ataaagtttc tttttatact gctccgtctg aaggtataca acgcttaacg
     4381 atattaccaa gtgcgttgtc cagagctatt tttccaagat taagttcaga attgcaatcg
     4441 gtaaagcaaa ctaaaatatt atcatatttt ataatggtta ttggtatact tccaattgta
     4501 atgttgataa ttattttatc agattttata atgtccgctt ggatgggacc tacatatcat
     4561 gggacgccag gtatagtatt aaaaattctt gcaataggtt tcttttttaa ttgcattgca
     4621 caaatcccat ttgtttcagt tcaggctagt ggaagatcaa aaattacagc tattattcat
     4681 ttgctcgaag ttatcccata tttatgcata ttatatattt ttatttatca ttggggaatt
     4741 gttggagccg caatagcatg gtctgtaaga acatcgttag attttttgat attattatta
     4801 attgatacga aatattaata gcgaattgat tttagggatt acttcctcaa gcccatctaa
     4861 ttagagtgca aacatgactt ctgattttta taactcaaaa gacaaaagtt taagtgttct
     4921 tttgtttttt gggtttatat ttttccttac acgtagcttt ccatttattc aatatagttg
     4981 gattatggag gggtttttat gtctttgtat catgtcattt acaaagaaaa ttgcaaacgg
     5041 aatatatcac tatcctgtta ttttaatatt tctattagct ctttttataa attttattta
     5101 ttcctatatc aagggtaacg atatagcgat aataattagg ttttatatta tcatattatt
     5161 tatattatgt gcttatttct gctcttatgg aaccatctcg attgttaaaa tatttttata
     5221 tttaatggta ttacaggcgg ttattatatc catcattagt atttatatga caaaaacata
     5281 tggtattggt gattattcag cactaagaca ttattttttg gagaatgatt atggtgatgt
     5341 ttatacatat ggaagtggtt tctatagagt tcaaattaaa ggaaatgctc tcattccatt
     5401 tgcctttatg ttgcatatag tcataaaaga ttatttctat tatcgattca aaaatacaat
     5461 aaccgttatt ctggctatag gtactatagt ggctggtaat tttgcatatt ttgtttcgat
     5521 atgcttgttt tttatgtata ttatactatg ttctaaatct aactcacgat acgctaaatt
     5581 aaggaaaatt atttttgggg tttttcttac tgtgattctc ccttttttta ttacatattc
     5641 aattgagttg ataatcatga aatcaaatgg agctgattct tctttaggag ttagatggga
     5701 tcagtttact gtattaatta atgatcttac agagtctgta tcaaattttg ttataggttc
     5761 tggtttgggt aatgtcatca aaattcaaac tcctatccgt gattatagtg catatatata
     5821 ttatgaattg cagtcagttt attttttaaa tcaacttggc gttattttat ttactttgtt
     5881 tttattaatt aatctccttc tcacgattaa aatcataaaa tacagtgagt tgtgtgtgct
     5941 atattttcta tatgtttctt atgcaattac taatccttat attttagact ctaaccatgt
     6001 tgctgtaata attgtattag tgacattaag taatgttcta aaaaagatga aagctaaatg
     6061 aaggttttaa ggtgaagatg gacactgtat atgccgtttt ggttgcttac aacccagaac
     6121 ataatgattt aaaaaatgcg gttgaattat tgttgagaca agttactaaa gttgtcgttt
     6181 gcaataactc tacaaatggt tataaatatg ctgaaaattc ttcaggcgat gtaaaaatat
     6241 tcaatttcaa tgataattta ggcatagcag aagcccaaag tataggaatg aaatgggctt
     6301 ttgaaaatgg cgctgatttt atattgcaaa tggatcagga tagtattcct gatcctaaga
     6361 tggtagagca gttacttact tgttacaaaa aattgcttaa acaaaatgtc aatgttggtt
     6421 tagttggttc acaagatttt gataaagtaa ctggtgaatt aaataaagca agggtaaaaa
     6481 aagggaaacc acttacagaa gtttattatg aggtagatag tacattaagt tctggcagtc
     6541 taataccaaa aaatagttgg ttgattgttg gaggaatgaa agatgagctt tttatcgatg
     6601 cggtagacca tgaatattgt tggagattaa gagctgctgg gtttaaagta attaggaata
     6661 aaaatgcgtt acttgcacat agacttggag atgggcgatt taagatctta aatattcttt
     6721 ctgtcggttt gccaagccca tttcgtcatt attatgctac tcgaaatatc tttcttttat
     6781 taaataaaaa ttatgtaccc atctactgga aaatttctag tctggttaaa ttaattggaa
     6841 aggttttttt atatcctatt ttccttccaa atggtaataa aaggttatat ttttttttaa
     6901 aaggcattaa tgacggttta atgggtcgaa gtggtaaaat gaaatgaatc atagattaga
     6961 aaaattctca gttttaatta gcatttataa aaatgatcta ccgcaatttt ttgaggtggc
     7021 tctacgctct atttttcacg atcaaacact taagccagat caaatagtaa ttgttgcaga
     7081 tggagaactc catcaaacac acatcgatat tataaattca ttcattgatg atgttggcaa
     7141 taaaatagta acatttgtac ctttacctag aaatgttgga ttggctaatg ccttaaatga
     7201 aggattaaag gcttgtagga atgagttagt ggcaagaatg gatgctgatg atatttcttt
     7261 gcctcatcgg tttgagaaac aaatttcttt tatgattaat aattcagaaa tagatgtatg
     7321 tggcagtttt attgatgaaa ttgaaactgt tactgaggag tttatttcaa cacgcaaagt
     7381 gcctctcgaa catagagaaa tagttaaatt cgcgaggaaa cgaagcgcag ttagccatcc
     7441 ttctgtaatt tttagaaaga atacagtatt agctgttggt ggttatcctc cattcagaaa
     7501 atctcaagat tttgcattgt ggagcctatt aattgtacat aatgcaagat ttgcaaatct
     7561 tccagatatt ttattaaaaa tgcgaactgg tcgtaatctt atggctcgac gtggattgtc
     7621 atatttattg tacgagtata aagtattgta ttatcaatat aaaattggtt ttattcgaaa
     7681 aaatgaatta ataagtaatg ctatgttgag aacatttttt cgtataatgc catctaaatt
     7741 aaaggagctg atgtattcaa tcgttaggaa tcgataataa taattttctg attaagtgtt
     7801 atggatttat ttttattagg catattctat aattaagcat aacccgcata ccacccagcg
     7861 gtatcctgac aggagtaaac aatgccaagc aacagatcgg cgtcgtcggt atggcagtg
//
