LOCUS       AF285971               13660 bp    DNA     linear   BCT 14-JUL-2016
DEFINITION  Shigella sonnei plasmid Pinv O antigen gene cluster, complete
            sequence.
ACCESSION   AF285971
VERSION     AF285971.1
KEYWORDS    .
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13660)
  AUTHORS   Shepherd,J.G., Wang,L. and Reeves,P.R.
  TITLE     Comparison of O-antigen gene clusters of Escherichia coli
            (Shigella) sonnei and Plesiomonas shigelloides O17: sonnei gained
            its current plasmid-borne O-antigen genes from P. shigelloides in a
            recent event
  JOURNAL   Infect. Immun. 68 (10), 6056-6061 (2000)
   PUBMED   10992522
REFERENCE   2  (bases 1 to 13660)
  AUTHORS   Shepherd,J., Wang,L. and Reeves,P.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2000) Microbiology, The University of Sydney,
            Sydney, NSW 2006, Australia
FEATURES             Location/Qualifiers
     source          1..13660
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="53G1"
                     /db_xref="taxon:624"
                     /plasmid="Pinv"
     gene            36..1142
                     /gene="wzz"
     CDS             36..1142
                     /gene="wzz"
                     /note="O antigen chain length determinant"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz"
                     /protein_id="AAG17417.1"
                     /translation="MSKASEPQQTPYLIPQGVYPTYMPKAEDEIDLFELLGTLWKKKW
                     VILCVTLLTTGLAAVYAFTAKEQWTAKTYIQAPRIAELGSYLKFHQAYARILNQPLDT
                     NALANGLFSDLILIAESPDTKVKFLESTEYYKKETNNLSTEQDKKIWLAEQANKGLVI
                     TPPKEKGNTSYYIIQASADSAQEAYKLLQGYLKNVNNQAVTLSLDEFGQNVNTLLVNL
                     NKEIIDIDFQRKSEKLDQIAHIQRDLTTAEQAGIIDYRSSKGGFDNAQSSYKFLLGEK
                     LLSAELKATKDAPIIYPFRYYEVKRQIDELEGMLRDNIQAQAYRYQMKPSEPVIKDKP
                     NKALILILGALPGAMFAIVGTLVYATLKDKTKLD"
     gene            1178..2491
                     /gene="wbgT"
     CDS             1178..2491
                     /gene="wbgT"
                     /codon_start=1
                     /transl_table=11
                     /product="WbgT"
                     /protein_id="AAG17418.1"
                     /translation="MFYVHFMMDKKMKFDTLNAKIGIIGLGYVGLPLAVEFGKKVTTI
                     GFDINKSRIDELRNGHDSTLECSNLELLEATKLTYACSLDALKECNVFIVTVPTPIDK
                     HKQPDLTPLIKASETLGKIIKKGDVIIYESTVYPGATEEDCIPVVEKVSGLKFNIDFF
                     AGYSPERINPGDKEHRVTNILKVTSGSTPDVAEYVDQLYKLIITVGTHKASSIKVAEA
                     AKVIENTQRDVNIALINELSIIFNKLGIDTLEVLEAAGTKWNFLPFRPGLVGGHCIGV
                     DPYYLTHKAQSVGYHPEMILAGRRLNDSMGQYVVSQLVKKMLKQRIQVEGANVLVMGL
                     TFKENCPDLRNTKVIDIISELKEYNINIDIIDPWCSTDEAQHEYGLTLCEDPKVNHYD
                     AIIIAVAHNEFREMGESAIRALGKDEHVLFDLKYVLDKKSIDMRL"
     gene            2572..3609
                     /gene="wbgU"
     CDS             2572..3609
                     /gene="wbgU"
                     /codon_start=1
                     /transl_table=11
                     /product="WbgU"
                     /protein_id="AAG17419.1"
                     /translation="MDIYMSRYEEITQQLIFSPKTWLITGVAGFIGSNLLEKLLKLNQ
                     VVIGLDNFSTGHQYNLDEVKTLVSTEQWSRFCFIEGDIRDLTTCEQVMKGVDHVLHQA
                     ALGSVPRSIVDPITTNATNITGFLNILHAAKNAQVQSFTYAASSSTYGDHPALPKVEE
                     NIGNPLSPYAVTKYVNEIYAQVYARTYGFKTIGLRYFNVFGRRQDPNGAYAAVIPKWT
                     AAMLKGDDVYINGDGETSRDFCYIDNVIQMNILSALAKDSAKDNIYNVAVGDRTTLNE
                     LSGYIYDELNLIHHIDKLSIKYREFRSGDVRHSQADVTKAIDLLKYRPNIKIREGLRL
                     SMPWYVRFLKG"
     gene            3698..4978
                     /gene="wzx"
     CDS             3698..4978
                     /gene="wzx"
                     /note="O antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AAG17420.1"
                     /translation="MFRKMIDAGGTFLLKAIFQIGVFVYFTHVSDITTFGIISYVFTV
                     YWFVLNFSDYGFRTKLVKDISDNSYSASELLSRSDGVKTYAFFFIFIIFMFYSYVSDS
                     ISLTLLVYISSAYFVCISSGRFSLLQAVGRFRCELYINIYSTIIYIGCNLFLSLFIEP
                     LYYSAISIFIYSISLLVFSSHKCNVPCFHIKRPSILVYKDFLDATPFAILVLLNVVLS
                     SIDLFILKEYFSYNSVAIYQVVTRVNTGLIIVFNVIYTVLLPSFSYYLKNSEWGNIRK
                     LQRYISLLVLLLCLCYYFFGIYFVGILFGDEYKVISSATFLIMFMALIKYNFWLINEL
                     YLVCSGNQSERVKSYCIGVVISMAVFFYFIPRYGWSGAVFGSAIATLVIGIFYIISVK
                     KDCGKILHDKYSLMMIFVPIFFYFIINGQQRLLY"
     gene            5047..6219
                     /gene="wzy"
     CDS             5047..6219
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AAG17421.1"
                     /translation="MLIYLYPVLLLFNILPVFFYGQMNSDLERFFGVPIGYIPDLIFY
                     FFVVLTSIITLRFHVSLWTKKLLFLGIIFLIYISIQMLLLSADISGVVILLSFFSNFI
                     ALVLLVSFCIGKDELYLTHSVRNINVVMCFGIICGVVKLFIGYSEDSNFIVYLNRNAT
                     AIIVVCFYCVYSYFYRGRKSWYVSSVLYSLFFLFLDSRAGIISFAISLFFVFLQLTKK
                     EKLLISLFFVPLLTLGISFTDIGTRLERMLSSSQVIFSGGNTLTKSQNDYRRVELVFI
                     GVDVLKENYLIGTGLGVANYVKAIDKKFLGSTNFGLAHNFYLSYSAQLGIIGFILLIS
                     VFYIMLSPIFKCGGYIGKGCVFALAFYVFFNEYILTPAIYIYISIFLSVVFIRNSK"
     mobile_element  6251..7367
                     /mobile_element_type=insertion sequence:IS630
     gene            7377..8621
                     /gene="wbgV"
     CDS             7377..8621
                     /gene="wbgV"
                     /codon_start=1
                     /transl_table=11
                     /product="WbgV"
                     /protein_id="AAG17422.1"
                     /translation="MLLEYVERKISLALSKYPKVRDVIKFFYLYIASLFAIILNKNKT
                     VIQSKIYEISIDDSEESFFGYYDHSPMSSNGRYVLFHSSAFSTKRHPKKVKYISICVK
                     DLLNNKVYKLYDTRAFNWQQGSRLMWIDDDNIIFNDYENNGYISVVYSLSLMKVIKKI
                     NYPIYDVNNYKAVTLDFSWLAKYDSDYGYYNKKSFSTDISIINLNTGGIELFLSLDEM
                     LKRTNFKCNIDVEHVVNHFMFAPDGRSVMFIHRYYTPKGKRERLIHWNLINDNVRVLI
                     NESIISHCCWNGNDEIIGFFGAEIDSLNYYRLSIESCNTEKLFFDARKYSDGHPTIVH
                     NRYIISDTYPDKNRIKKLFVYDLVKNDYRELGLFYESLSFFSYSRCDLHPRISVDNRF
                     LFVDSVHSGKRKLYFMRSGICE"
     gene            8605..9600
                     /gene="wbgW"
     CDS             8605..9600
                     /gene="wbgW"
                     /note="putative glycosyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAG17423.1"
                     /translation="MVFVSDVLVSLIIVCFNAEKYIEKSLLAFINQDVGLDKFELIIV
                     DGDSSDNTISIVQDVFSKHSNIKHKIINNKKRTLATGWNIGVLEANGKFVCRVDAHSD
                     IPNNYISKLLDDYFNIMQFDDSVVGVGGVLTNSYKTKFGSIVADFYASKFGVGNSPFR
                     CVDKNNRLKKTDTAVFALYNKDVFFDVGLFNEVLDRNQDIDFHKRVLSNNLSLYTDNS
                     LFVEYYVRDNFKDFIKKGFLDGFWVVMSGAYYFRHIVPLFFVLYLIVSFSLFFATGDY
                     IYLSFLFFYFLISILFSIRDGRSFIGRVFLPFIFLSYHISYGCGSLLSFLKRYFK"
     gene            9597..10751
                     /gene="wbgX"
     CDS             9597..10751
                     /gene="wbgX"
                     /codon_start=1
                     /transl_table=11
                     /product="WbgX"
                     /protein_id="AAG17424.1"
                     /translation="MKNFIPFALPEIGEEEIAEVIDSLRSGWITTGPKAKQFEQEFSN
                     YLGANVQSLAVNSATSGLHLALEAVGVKPGDQVIVPSYTFTATAEIVRYLGADPVIVD
                     VDRKTFNISVDAIEKAITNETKAIIPVHFAGLACDMDSILSIAKKYDLKVVEDAAHAF
                     PTTYKGSKIGTLDSDATVFSFYANKTMTTGEGGMVVSKNKDIIERCKVMRLHGISRDA
                     FDRYQSKTPSWFYEVVAPGFKYNMPDICAAIGIHQLRKIDDFQKKRQRMAKIYDDALK
                     ELPLELPEWPTNASDIHAWHLYPIRLKTDSAINRDDFIKKLSDLGIGCSVHFIPLHKQ
                     PVWRDTYNLNASDFPVSEECYLNEISIPLYTKMTDQDQLFVIKSIRQLFM"
     gene            10768..11358
                     /gene="wbgY"
     CDS             10768..11358
                     /gene="wbgY"
                     /note="putative glycosyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAG17425.1"
                     /translation="MKRIFDVIVAGLGLLFLFPVFIIVSMLIVADSKGGVFFRQYRVG
                     RFGKDFRIHKFRTMFIDSEKKGRITVGQDARVTRVGWYLRKYKIDELPQLIDVLSGTM
                     SLVGPRPEVREFIDEYPDDIREKVLSVRPGITDLASIEMVDENEILSSYDDPRRAYID
                     IILPIKQRYYLDYVANNSVKYDCVIIWKTIIKILSR"
     gene            11373..13292
                     /gene="wbgZ"
     CDS             11373..13292
                     /gene="wbgZ"
                     /codon_start=1
                     /transl_table=11
                     /product="WbgZ"
                     /protein_id="AAG17426.1"
                     /translation="MIDRILELPRIVKRGIIICIDVVMVIFSFWLSYWLRLDEQTAFL
                     SAPMWFAAAILTIFTVFIFIRIGLYRAVLRYVSAKIMLLIPVGILASTLSLVVISYSL
                     SIMLPRTVVGIYFLVLLLLTSGSRLLFRMILNYGVKGSAPVLIYGAGESGRQLLPALM
                     QAKEYFPVAFVDDNPRLHKAVIHGVTVYPSDKLSYLVDRYGIKKILLAMPSVSKSQRQ
                     KVITRLEHLPCEVLSIPGMVDLVEGRAQISNLKKVSIDDLLGRDPVAPDAKLMAENIT
                     GKAVMVTGAGGSIGSELCRQIVRYKPAKLVLFELSEYALYAIEKELSALCDKEVLNVP
                     VIPLLGSVQRQNRLQMVMKSFGIQTVYHAAAYKHVPLVEHNVVEGVRNNVFGTLYCAE
                     SAIESGVETFVLISTDKAVRPTNTMGTTKRLAELVLQALSARQSQTRFCMVRFGNVLG
                     SSGSVVPLFEKQIAQGGPVTLTHRDIIRYFMTIPEASQLVIQAGAMGHGGDVFVLDMG
                     DPVKIYDLAKRMIRLSGLSVRDDKNPDGDIAIEVTGLRPGEKLYEELLIGDSVQGTSH
                     PRIMTANEVMLPWQDLSLLLKELDQACHDFDHERIRSLLLQAPAAFNPTDDICDLVWQ
                     QKKSLLSQASNVIRL"
BASE COUNT     4012 a   2103 c   2869 g   4676 t
ORIGIN
        1 atttttaacc cattgataaa taatggattg gtttcatgtc aaaagcatct gaaccacaac
       61 agacccctta tctgatcccg caaggggtct atccaactta tatgccaaaa gcagaggatg
      121 aaatcgatct tttcgagctt ttaggcacct tgtggaagaa aaaatgggtt attttatgtg
      181 tcacgttgct gactacgggg ttagcagcag tgtatgcctt taccgcaaaa gagcagtgga
      241 cggctaaaac ctatattcaa gcaccacgta ttgctgaatt aggcagctat cttaaatttc
      301 accaagcgta tgcccgaata ttaaatcaac cgttagatac gaatgcgttg gctaatggat
      361 tgttttccga tttgattttg attgctgaat cgccagacac caaagttaaa tttctagaga
      421 gtactgagta ttataaaaag gaaacaaata atttatctac tgaacaagat aagaaaattt
      481 ggttagctga gcaagcgaat aaaggtcttg tgattacgcc accaaaggaa aagggaaata
      541 caagttacta cataatacaa gcatcggcag actcagcgca agaggcatat aaactactgc
      601 agggatatct aaagaatgtt aataatcaag ctgtaacatt aagtcttgat gagtttggtc
      661 aaaatgttaa tactcttttg gttaatctaa ataaagaaat tattgacata gatttccaga
      721 gaaaatcaga aaagcttgat caaatagctc atattcagcg agatttaaca actgcggaac
      781 aagccggaat cattgattat cgctctagca aaggcggctt cgataatgcg caaagtagct
      841 ataagttctt gctcggcgaa aaactgttat cagcagagct aaaagcaact aaagatgcgc
      901 caattattta cccatttaga tattacgaag tgaaacgtca aattgatgag ttagaaggaa
      961 tgttacgcga taacattcag gcgcaagcat atcgatatca aatgaagcca tctgagccag
     1021 ttataaaaga caaacccaac aaagcattaa ttttgattct tggtgcatta ccaggggcaa
     1081 tgtttgctat agttggtaca ttagtttatg cgacattaaa agataaaacc aagttagatt
     1141 aaactgggtt acgtattgtt gtgtcaatgc gaaatagatg ttctatgtgc actttatgat
     1201 ggataagaaa atgaaattcg atactttgaa tgcgaaaatt gggattatag gccttggtta
     1261 tgttggattg cctcttgctg ttgagtttgg aaagaaagta acgacgattg gatttgatat
     1321 taataagtct cgtattgatg aattacgaaa tggtcacgat agtacattag agtgctcaaa
     1381 tttagagttg ttagaagcaa ctaaattgac gtacgcctgt tcattagatg cactaaaaga
     1441 gtgtaatgta tttattgtaa ctgttccaac tccaattgat aaacataaac agccagatct
     1501 aacacctcta attaaagcat ctgaaacatt gggtaagata ataaagaaag gcgatgttat
     1561 tatttatgag tcaacagttt accctggagc gacagaagaa gattgtatac cagttgtaga
     1621 gaaagtatca ggtcttaagt ttaatattga tttttttgcc ggttattcac ctgagcgtat
     1681 taatcctggg gataaagagc atcgtgtaac taatatcctt aaggtgacca gtggatctac
     1741 accggatgtt gctgagtatg tagatcagct atataaatta ataattactg tcggtacgca
     1801 taaagcatca tcgataaaag tagcagaggc tgcaaaagta attgaaaaca cgcagcgaga
     1861 tgtcaatatt gcattgatta atgagttatc tattatattt aataagttag ggattgatac
     1921 cttagaggtt cttgaggctg caggtacgaa gtggaatttt ttacctttta ggcccggttt
     1981 agtaggtggc cactgtatag gtgtagatcc ttattatctt acacataaag cgcaaagtgt
     2041 cggctatcat ccggagatga ttttagccgg acgtcgttta aatgatagta tggggcagta
     2101 tgtcgtttcc cagttagtca aaaaaatgtt gaaacaacgg attcaagttg aaggggcgaa
     2161 tgtgttagtg atggggctta catttaaaga gaattgccca gatctacgaa acactaaagt
     2221 gattgatatt atttcagagt taaaagaata caatatcaat atagatatta tagatccatg
     2281 gtgttctacc gatgaggcac aacatgaata tggattaact ttatgtgaag atcctaaagt
     2341 taatcattat gatgcaataa ttatcgctgt tgcacacaat gagtttcgcg agatgggaga
     2401 gagcgctatt cgtgcattag gtaaagacga gcacgttttg ttcgatttaa aatatgtgct
     2461 tgataaaaaa agtatcgata tgcgcttgta agagtgatta aaaaaatcaa atcctctttg
     2521 atatgataca cctcagcatt ttatgctagg tttagcactt gattaatata catggatatt
     2581 tatatgtctc gctatgaaga gattacacag cagttaattt tttcaccgaa aacttggtta
     2641 attactggtg tcgctggctt tataggatca aatcttttag aaaagttact taaattaaac
     2701 caggttgtta ttgggttaga taacttttcc acgggacatc aatataatct tgatgaagtt
     2761 aaaacattag tttccactga acagtggagt cgattttgct ttatagaagg tgatattcga
     2821 gatctcacta cctgtgagca agttatgaaa ggtgttgatc atgtcttaca tcaggctgcg
     2881 ctaggttctg tacctcgttc aattgttgat cctataacaa ccaatgcaac taatattact
     2941 ggatttttga atatcttaca tgcggctaaa aatgcacaag tacaaagttt tacttatgct
     3001 gcatcaagct caacttatgg agatcatccc gcactaccaa aagtagagga aaacattggt
     3061 aatccacttt ctccttatgc agttactaaa tatgttaacg agatttatgc tcaggtatat
     3121 gctcgaacat atggttttaa aactattgga ttacgttatt ttaatgtatt tggtcgtcgt
     3181 caagatccta atggagctta tgctgcagta attccaaaat ggacagcagc aatgcttaaa
     3241 ggtgatgacg tatatattaa tggcgatggt gaaacgagtc gtgatttttg ttatatagat
     3301 aatgttatac aaatgaatat attatctgca ttagcgaagg acagtgctaa agataatata
     3361 tataatgttg cagttggtga tagaacaacg ttaaatgaat tatctggtta catttatgat
     3421 gagcttaatt taattcacca tatcgataaa ttgagcatta agtatagaga gtttagatct
     3481 ggagatgtta ggcattctca ggctgatgtt actaaggcta tagatttact aaagtataga
     3541 ccaaatataa aaatcagaga gggattacga ctttcaatgc cgtggtatgt gagattttta
     3601 aaaggctaaa ttatattaac atgaataaat aatctatttc acctctgtta ttaatgcagg
     3661 ggtgaaaatc catgtattta ttctaaatgg tcagtgtatg tttagaaaaa tgattgatgc
     3721 aggtggtaca tttttactta aagcaatatt tcaaatagga gtttttgttt atttcacaca
     3781 tgtgtcagat attactacat ttggtattat tagttatgtg tttactgttt attggtttgt
     3841 gcttaacttc tctgattatg gatttagaac aaaattagtg aaagatattt ctgataatag
     3901 ttattctgca tcagaattat tatcaagaag tgatggagtt aaaacatatg cttttttctt
     3961 catttttata atcttcatgt tttattctta tgtttctgat tcaatttcat taactctgct
     4021 tgtttatatt tcatctgcat attttgtttg tatttcaagt ggtagattta gcttgctaca
     4081 ggctgttggt cggtttagat gtgaattata tataaatatc tactcaacaa ttatatatat
     4141 tgggtgtaat ttatttttat ctctgtttat cgaacctcta tattatagtg cgatatcaat
     4201 attcatatac tcaatttcgc ttttggtttt ctcatcacat aaatgcaatg tgccatgttt
     4261 tcatataaaa agaccaagta ttttagttta taaagatttt ttggatgcaa ctccgttcgc
     4321 tattctggtg ttactaaatg ttgttttatc tagtattgac ctttttatat taaaagaata
     4381 tttctcttat aatagtgttg ctatatatca ggtggtaact agggttaata ccggtctaat
     4441 aatagtgttt aatgttattt atactgtttt attgccttca ttttcttatt atctgaaaaa
     4501 ttctgaatgg ggtaatataa ggaaattaca acgatatata tcactgttag tcttattact
     4561 atgtttatgc tattattttt ttggcatcta tttcgtaggg atattgtttg gtgatgagta
     4621 taaggtaata tcttctgcaa catttttgat aatgtttatg gctcttatta aatataattt
     4681 ttggctaata aatgaacttt atcttgtgtg tagtggaaat caaagcgagc gagttaaatc
     4741 gtattgtatt ggtgtggtca tttcaatggc ggttttcttt tattttatac ctcggtatgg
     4801 atggagtggg gcggtttttg gaagtgccat tgcaacatta gtaattggaa tattttatat
     4861 tatttctgtg aaaaaagatt gtgggaaaat tcttcatgat aagtattcac taatgatgat
     4921 ctttgtccca attttctttt attttattat taatggtcag cagcggttgt tatattaata
     4981 tgttgtggtt ttatatcgtt ccattaatat gtttagactc gattgaaagc ctaataaagg
     5041 ttaagtatgt taatatacct atatcctgta cttttgttat ttaatatcct tccggttttt
     5101 ttttatggac aaatgaactc tgatttagag cgtttttttg gagttcctat tggctatatt
     5161 ccagatctaa tattttattt ctttgttgtt ttaacatcta taataacgtt gaggtttcac
     5221 gtttctctgt ggacaaagaa attattattt ttaggcatca tattcctgat ttatatcagc
     5281 attcagatgt tgttgttatc agcggatata tcaggtgtcg taattttatt atcgtttttt
     5341 tctaatttta tagctttggt tcttttggtg tcattttgca ttggtaaaga tgagctttat
     5401 ttaactcatt cggttagaaa tataaatgtt gtaatgtgtt ttggtattat ctgtggagtt
     5461 gtaaaattat ttattggtta ttctgaagat agtaatttta tagtttattt aaatagaaat
     5521 gccaccgcaa ttatagtagt gtgcttttat tgtgtatatt catactttta tcgtggtcga
     5581 aagtcttggt atgtctcatc tgtattgtac tctctgttct ttctttttct ggatagccga
     5641 gcaggaataa tatcatttgc tatatcgttg ttttttgttt ttcttcagtt aacaaagaag
     5701 gaaaagttat taatatcatt gttttttgtt cctcttctaa ctttaggtat ttcttttact
     5761 gatataggca ctcgtcttga acgaatgctg tcttcgtcac aggttatatt ctctggtggt
     5821 aacactctta caaaaagtca gaatgattat cgtcgagttg agttagtatt tattggggtt
     5881 gatgttttaa aagaaaatta tttaattggc actggattag gtgttgcaaa ttatgtaaag
     5941 gctatagata aaaagttttt aggaagtacc aactttgggt tggcgcataa tttttattta
     6001 tcttattcgg ctcagttagg gattattggt tttattttgc ttatttctgt attttatata
     6061 atgctgtctc caatttttaa atgcggaggg tatattggta aaggatgcgt ttttgctttg
     6121 gctttctatg tcttttttaa tgagtatata ttgacgccag cgatatatat ttatatttct
     6181 atttttttat cggtggtttt tatacgtaat tctaaatagc tgcgcggaat agtagatcac
     6241 tttgagggaa cttagcccgg attgtgcgat ctgatcaatc gccaaatcaa aacaaatcac
     6301 caaccggact gagcaatgcc gatcatagca ccaatttccc gtgacgaacg acgcctgatg
     6361 cagaaagcca tccataaaac acacgataaa aattatgccc gcagactgac tgccatgctg
     6421 atgctgcacc ggggcgaccg tgtcagcgac gttgccagaa cgctctgctg cgcccgttcc
     6481 tctgttggac gctggattaa ctggttcacg cagtcgggtg ttgagggact gaaatcatta
     6541 cctgccgggc gtgcccgtcg ctggccgttt gagcatatct gcacactgtt acgtgagctg
     6601 gtaaaacatt ctcccggcga ctttggctac ggttcacgct ggagtacaga actgctggca
     6661 ataaaaatca atgagataac cggttgccag ttaaatgccg gaaccgttcg ccgctggttg
     6721 ccgtctgcgg ggattgtgtg gcgaagggct gcgccaactc tgcgtatccg tgacccgcat
     6781 aaagatgaaa agatggcagc aatccataaa gcactggacg aatgcagcgc agagcatccg
     6841 gtcttttatg aagatgaagt ggatatccat cttaatccca aaatcggtgc ggactggcaa
     6901 ctgcgcggac agcaaaaacg ggtggtcacg ccgggacaga atgaaaaata ttatctggcc
     6961 ggagcgctgc acagcgggac aggtaaagtc agctgtgtgg gcggcaacag caaaagttcg
     7021 gcgctgttca tcagcctgct gaagcggctt aaagcgacat accgtcgggc gaaaaccatc
     7081 acgctgatcg tggacaacta cattatccac aaaagccggg aaacacagag ctggctgaag
     7141 gagaacccga cgttcagggg tcatttatca gcggtttact cgccatggat gaatcatgtt
     7201 gaacggctat ggcaggcact tcacgacaca ataacgcgta atcatcagtg cagctcaatg
     7261 tggcaactgt tgaaaaaagt tcgccatttt atggaaaccg tcagcccatt ccccggaggc
     7321 aaacatgggc tggcaaaagt gtagcggtat taagcgcagc tatttaggat gagaatatgt
     7381 tgttagaata tgttgaaaga aaaatttcct tagccttgag taagtatcct aaggtaaggg
     7441 atgttattaa gttcttttat ttatatatcg catcattatt cgcaattatt ttgaataaaa
     7501 ataagacggt tattcaatca aaaatatacg agatttcaat tgatgattct gaagaatcat
     7561 tttttggcta ttatgaccat agtccaatga gctctaatgg gcggtacgta ttgttccact
     7621 ctagtgcgtt tagcactaaa cgacatccaa agaaagttaa gtatatatct atttgcgtaa
     7681 aagaccttct taataacaaa gtttataagc tatatgatac gcgagcattt aattggcagc
     7741 agggaagccg attaatgtgg attgatgatg acaatataat ttttaatgac tatgaaaata
     7801 atggatacat tagtgttgtc tattctttgt ctttgatgaa ggttataaaa aaaataaact
     7861 atccgattta tgatgtgaat aattacaagg ctgtgacgtt agatttctca tggctggcta
     7921 aatatgatag cgattatggt tattataata aaaaatcatt ttctacagat atttcaatca
     7981 ttaatttgaa cacgggggga atagaattat ttttatcctt agacgaaatg ctaaagagaa
     8041 ctaattttaa atgtaatatt gatgttgaac atgtggtcaa tcattttatg tttgctcccg
     8101 atggacgttc cgttatgttc atacatcgat actatacacc taaaggaaag cgtgaaaggt
     8161 taatacattg gaatttaata aatgataatg ttcgagtcct aataaatgaa tcgattatta
     8221 gtcattgttg ttggaatggg aatgatgaaa ttataggttt ttttggtgca gaaatagatt
     8281 cgctaaatta ttatagattg tcaattgaat cctgtaatac agagaaattg ttttttgatg
     8341 caagaaaata ttctgatgga catcctacta tagttcataa tagatatatt atatctgata
     8401 cttacccaga taaaaataga attaaaaagt tgtttgttta tgaccttgtc aaaaatgatt
     8461 atcgcgagct tggattattt tatgagtcat tgagtttttt ttcttattct cgatgtgact
     8521 tacatccaag gatctcggtt gataatagat ttttgtttgt tgattcagtt cactcaggga
     8581 aaagaaaact atattttatg aggagtggta tttgtgagtg atgttctagt atctttaatt
     8641 atagtttgct ttaatgcaga gaagtatatt gaaaaatctc ttttggcatt tattaatcaa
     8701 gatgttggat tagataaatt tgaattgatt attgtagatg gggattcatc tgataataca
     8761 atatctattg ttcaggatgt tttttctaaa catagcaaca ttaagcataa aattatcaat
     8821 aataaaaaaa gaactcttgc tacgggttgg aatattgggg tgctagaagc taatggtaag
     8881 tttgtgtgta gagttgatgc acatagtgat attccaaata actatatatc taaattatta
     8941 gatgattatt ttaatattat gcagtttgat gatagcgttg ttggtgttgg aggtgtatta
     9001 actaattctt ataaaactaa gtttggttca attgtagcgg atttttatgc atcgaaattt
     9061 ggtgttggta attctccatt taggtgcgta gacaaaaata atcgactaaa aaaaacagat
     9121 acggctgtct ttgctttata taataaagat gtgttttttg atgttggact ttttaatgaa
     9181 gtattagata gaaatcaaga tattgatttt cataagagag ttttaagcaa taatttgtca
     9241 ttatatacag ataatagttt atttgttgag tattatgtta gagataattt taaagatttc
     9301 ataaagaaag gttttcttga tggtttttgg gttgttatgt ctggagcata ttattttaga
     9361 catatagtgc cacttttttt tgttttgtat ttaattgtat ctttttctct tttctttgct
     9421 actggtgatt atatatattt atctttttta tttttttatt ttcttatttc tattttgttt
     9481 tcaattcgag atgggcgaag ttttataggt agagtatttc ttccttttat atttttgtct
     9541 tatcatattt cttatggatg tggatcgtta ttatcttttt tgaaaaggta ttttaaatga
     9601 aaaattttat tccttttgcg ttacctgaaa ttggcgaaga agaaattgca gaggtaattg
     9661 actctttacg ttcaggttgg attacgacag gtcctaaggc taagcaattt gaacaagaat
     9721 tttctaatta cctaggagcg aacgttcaat cattagctgt taactctgct acgtcgggct
     9781 tacatttggc tcttgaagct gttggcgtaa agccgggaga ccaagttatt gtcccatcat
     9841 atacattcac tgctactgcc gaaattgtca ggtaccttgg tgctgatcct gtaattgttg
     9901 atgtagatcg taaaacattt aatatatcag ttgatgccat tgagaaggct attactaatg
     9961 aaacaaaggc gattattcca gtacacttcg ctggattagc ttgtgacatg gattcaatct
    10021 tatcaattgc taaaaaatat gacctaaagg ttgtcgagga tgccgctcat gcatttccta
    10081 caacatataa aggaagtaag ataggaacgc ttgattcaga tgctacggtt tttagcttct
    10141 acgccaataa aactatgaca accggtgaag gcggaatggt tgtttcaaaa aataaagata
    10201 taattgagcg ttgtaaggta atgcgtttac atggaatcag tcgtgacgct tttgaccggt
    10261 accagtctaa aactccttct tggttttatg aggttgtagc tccagggttt aaatacaata
    10321 tgcctgatat ctgtgcggca atcggtattc atcaacttag aaagatcgat gattttcaga
    10381 aaaaacgtca acgaatggca aaaatttacg atgatgcgtt aaaagaattg ccacttgaat
    10441 tgcctgaatg gcctactaat gctagtgata ttcatgcttg gcatctatat cctatccgct
    10501 taaaaactga ttcggctatt aatcgcgatg attttattaa gaagttatca gatcttggaa
    10561 ttggttgttc tgtccatttt ataccgttgc ataagcaacc ggtttggcgt gatacatata
    10621 atttgaacgc cagtgacttt ccagtttctg aggagtgtta tttaaatgaa atatctattc
    10681 ctctttatac taaaatgacg gatcaagatc agttgttcgt tatcaaatcg attagacaat
    10741 tatttatgta atggtatttt atattaaatg aaacgtattt ttgatgttat cgtggcaggc
    10801 ttaggcctgc tttttctatt tcctgttttt atcattgtgt caatgttaat tgttgctgat
    10861 tctaaagggg gggttttttt taggcagtat agagttggga gatttgggaa agattttagg
    10921 atacataaat ttagaacgat gtttatcgat tcagaaaaaa aaggacggat aacagttggt
    10981 caagatgctc gggtaaccag agttggatgg tatttacgga agtacaaaat cgatgagctt
    11041 cctcaattga tagatgttct ttctggaaca atgagtttgg ttggcccaag accggaagtg
    11101 agggagttta ttgatgagta tcctgatgat ataagggaaa aagttttatc ggttaggcca
    11161 gggataactg acttagcatc tatagaaatg gtagatgaaa atgagatttt gtctagttat
    11221 gatgacccac gtagggctta tatagatata attcttccaa tcaagcaaag atattattta
    11281 gattatgttg ctaacaattc agtaaagtat gattgtgtga taatttggaa aactattatt
    11341 aagattttgt cgcgataata aggtagtgta ggatgattga tagaatattg gagctgccaa
    11401 gaattgttaa gagaggtatc atcatctgca ttgatgtagt tatggtgata ttctcatttt
    11461 ggttgtctta ttggttgagg cttgatgagc aaacggcttt tcttagtgca ccgatgtggt
    11521 ttgctgcagc tattcttacc atatttaccg tgtttatatt tatcaggatt gggctttatc
    11581 gggcagtctt acggtatgtt agtgcaaaga taatgttgct aataccagtt ggtattctgg
    11641 cctcaacgtt atctcttgtc gttatatcat attcgctatc cataatgttg ccgcgcactg
    11701 ttgtcggaat ttattttttg gttttacttt tactgacatc aggctctaga ttgcttttta
    11761 gaatgatact taactatgga gttaagggta gtgcgcctgt tttgatttat ggcgctggtg
    11821 aatctggccg acaattattg ccagcattaa tgcaggcaaa agaatatttt cctgtggcat
    11881 ttgtggatga taatcctcgc ttgcataagg ctgtcattca tggtgtaaca gtttatccct
    11941 cggataaact gagttacctt gtagatcgct atggtataaa gaaaattctt ttggcgatgc
    12001 cgagcgtcag taagtcacaa aggcagaaag tgattactcg tttagagcat ctaccgtgtg
    12061 aagttctctc tattccgggt atggtcgatt tagtcgaagg tcgagcacaa atcagtaatc
    12121 taaaaaaagt atcgattgat gacttactag gtcgtgatcc ggttgctcct gatgccaaat
    12181 tgatggccga aaacattact ggcaaagccg ttatggtcac tggggcggga ggctcgatcg
    12241 gctctgagct ttgtcgtcaa attgttcgat ataagccggc caaattggtt ctatttgaac
    12301 tgtctgaata tgccctctac gctattgaga aagagctctc ggcgctgtgt gacaaagaag
    12361 ttttgaatgt tccagtgatc cctctgttgg gctcggtgca gcgtcagaat cgcttacaga
    12421 tggtgatgaa gtcctttggt attcaaacgg tttatcatgc ggccgcttat aaacatgtgc
    12481 ctctggttga gcataatgtg gtggaagggg tacgtaataa cgtgtttggt accttgtact
    12541 gcgctgagtc agcgatcgaa agtggcgttg aaacttttgt gttgatttcc accgataaag
    12601 cggtgcgccc gaccaacact atggggacaa ctaagcgtct ggccgaattg gtattgcagg
    12661 ctttgtctgc acggcaaagc caaactcgct tttgtatggt gcgatttggt aatgtactcg
    12721 gttcttcggg ctctgtcgtg ccgttgtttg aaaaacagat tgcccaaggt gggccagtta
    12781 ccttgactca tcgtgacatt attcgctatt tcatgacaat tccggaagca tcacagttgg
    12841 tgattcaagc gggggcgatg gggcatggcg gcgatgtctt tgtcttagac atgggcgatc
    12901 cggtcaagat ttatgactta gccaaacgca tgatccggtt aagtggcttg agtgtacggg
    12961 atgataaaaa tccagatggc gatattgcca ttgaagttac gggattacgt ccaggtgaga
    13021 aactgtatga agaattactg attggtgatt cagttcaagg tacctctcat ccacgaatta
    13081 tgacggccaa cgaagtgatg ctaccgtggc aggatctatc gctcttactt aaagagctgg
    13141 atcaagcttg tcatgacttt gatcatgagc gaattcgcag tttgttgtta caagcaccag
    13201 cggcattcaa tccaactgat gatatttgcg atctagtttg gcagcagaaa aaatcgctgt
    13261 tatcacaagc gagcaatgtc attcgcctgt gattgcttag gtttaacctt ccacaccaat
    13321 tcttcacctc tcttacaaat ccccgctagg cggtacatcg tgaccgcctt taccctgatg
    13381 cctgctcttt aacaaacagg acatcagtgt atgtttaaac cttttagcgc cgaatttttc
    13441 ggcactttct ggctggttct gggtggctgt ggtagcgcct tgatctctgc tgctttccca
    13501 cagttaggta taggcttttt gggcgtggcg ttggcgtttg gtctgacagt agtcaccatg
    13561 gcttatgcgg tcgggcacat ctctggtgcg cattttaacc ccgcggtgac cttgggtctg
    13621 tgggccggtg gacgctttcc tgcagcgcgc gtgttacctt
//
