LOCUS       CR931634               10337 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Streptococcus pneumoniae strain 524/62 (serotype 3).
ACCESSION   CR931634
VERSION     CR931634.2
KEYWORDS    .
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10337)
  AUTHORS   Bentley,S.D., Aanensen,D.M., Mavroidi,A., Saunders,D.,
            Rabbinowitsch,E., Collins,M., Donohoe,K., Harris,D., Murphy,L.,
            Quail,M.A., Samuel,G., Skovsted,I.C., Kaltoft,M.S., Barrell,B.,
            Reeves,P.R., Parkhill,J. and Spratt,B.G.
  TITLE     Genetic analysis of the capsular biosynthetic locus from all 90
            pneumococcal serotypes
  JOURNAL   PLoS Genet. 2 (3), E31 (2006)
   PUBMED   16532061
REFERENCE   2  (bases 1 to 10337)
  AUTHORS   Bentley,S.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2004) Submitted on behalf of the Pathogen
            Sequencing Unit, Sanger Institute, Wellcome Trust Genome Campus,
            Hinxton, Cambridge CB10 1SA, E-mail: sdb@sanger.ac.uk
COMMENT     On Mar 17, 2006 this sequence version replaced CR931634.1.
            NOTE: This sequence was generated from a PCR product representing
            the region from dexB to aliA and is not necessarily responsible for
            the expressed capsule serotype. For a detailed description of how
            CDS products were predicted see the associated publication.
FEATURES             Location/Qualifiers
     source          1..10337
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="524/62"
                     /db_xref="taxon:1313"
     gene            <1..162
                     /gene="dexB"
                     /locus_tag="SPC03_0001"
     CDS             <1..162
                     /gene="dexB"
                     /locus_tag="SPC03_0001"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAI32738.2"
                     /db_xref="GOA:Q4K2R1"
                     /db_xref="InterPro:IPR013780"
                     /db_xref="UniProtKB/TrEMBL:Q4K2R1"
                     /translation="DRRFLVVANLSNEEQDLTVEGKVKSVLIENTLAQEVFEKQILVP
                     WDAFCVELL"
     gene            321..494
                     /gene="aliB"
                     /locus_tag="SPC03_0002"
                     /pseudo
     CDS             321..494
                     /gene="aliB"
                     /locus_tag="SPC03_0002"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative oligopeptide-binding protein AliB
                     (pseudogene)"
                     /db_xref="PSEUDO:CAI32739.2"
     gene            complement(order(487..813,827..1336))
                     /gene="tnp"
                     /locus_tag="SPC03_0003"
                     /pseudo
     CDS             complement(join(487..813,827..1336))
                     /gene="tnp"
                     /locus_tag="SPC03_0003"
                     /note="member of homology group 4;
                     old_tribe:0004 68"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative IS630-Spn1 transposase"
     misc_feature    complement(965..1336)
                     /gene="tnp"
                     /locus_tag="SPC03_0003"
                     /note="HMMPfam hit to PF01710, Transposase, score 7.2e-19"
                     /pseudo
     misc_feature    order(1523..1765,1766..1850)
                     /note="HMMPfam hit to PF02916, DNA polymerase processivity
                     factor, score 9.5e-52"
     gene            1524..2748
                     /gene="wzg"
                     /locus_tag="SPC03_0004"
                     /pseudo
     CDS             join(1524..1766,1765..2748)
                     /gene="wzg"
                     /locus_tag="SPC03_0004"
                     /note="member of homology group 0;
                     old_tribe:0000 80"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integral membrane regulatory protein Wzg
                     (pseudogene)"
     misc_feature    2025..2468
                     /gene="wzg"
                     /locus_tag="SPC03_0004"
                     /note="HMMPfam hit to PF03816, Cell envelope-related
                     transcriptional a, score 6.4e-68"
                     /pseudo
     gene            2750..3480
                     /gene="wzh"
                     /locus_tag="SPC03_0005"
                     /pseudo
     CDS             join(2750..3028,3028..3480)
                     /gene="wzh"
                     /locus_tag="SPC03_0005"
                     /EC_number="3.1.3.48"
                     /note="member of homology group 1;
                     old_tribe:0001 80"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase Wzh (pseudogene)"
                     /db_xref="PSEUDO:CAI32742.2"
     misc_feature    order(2753..3028,3028..3360)
                     /gene="wzh"
                     /locus_tag="SPC03_0005"
                     /note="HMMPfam hit to PF02811, PHP domain, score 3.5e-35"
                     /pseudo
     gene            3489..4181
                     /gene="wzd"
                     /locus_tag="SPC03_0006"
     CDS             3489..4181
                     /gene="wzd"
                     /locus_tag="SPC03_0006"
                     /note="member of homology group 2;
                     old_tribe:0002 80"
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Wzd"
                     /protein_id="CAI32743.2"
                     /db_xref="GOA:Q4K355"
                     /db_xref="InterPro:IPR003856"
                     /db_xref="InterPro:IPR005701"
                     /db_xref="UniProtKB/TrEMBL:Q4K355"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQGEKSGLTNQDLQAGSYLVKDYREIILSQDVLEEVISDLKLD
                     LTPKGLANKIKVTVPVDTRIVSVSVNDRVPEEASRIANSLREVAAQKIISITRVSDVA
                     TLEEARPAISPSSPNIKRNTLIGFLAGGIGTSVIVLLLELLDTHVKRPEDIEDTLQMT
                     LLGVVPNLGKLK"
     misc_feature    3507..3923
                     /gene="wzd"
                     /locus_tag="SPC03_0006"
                     /note="HMMPfam hit to PF02706, Chain length determinant
                     protein, score 3e-53"
     gene            4191..4604
                     /gene="wze"
                     /locus_tag="SPC03_0007"
                     /pseudo
     CDS             4191..4604
                     /gene="wze"
                     /locus_tag="SPC03_0007"
                     /EC_number="2.7.1.112"
                     /note="member of homology group 3;
                     old_tribe:0003 80"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wze (pseudogene)"
     gene            5173..5673
                     /gene="tnp"
                     /locus_tag="SPC03_0008"
                     /pseudo
     CDS             5173..5673
                     /gene="tnp"
                     /locus_tag="SPC03_0008"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1548 transposase"
                     /db_xref="PSEUDO:CAI32745.2"
     gene            5752..6936
                     /gene="ugd"
                     /locus_tag="SPC03_0009"
     CDS             5752..6936
                     /gene="ugd"
                     /locus_tag="SPC03_0009"
                     /note="member of homology group 20;
                     old_tribe:0020 15"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase Ugd"
                     /protein_id="CAI32746.2"
                     /db_xref="GOA:Q4K352"
                     /db_xref="InterPro:IPR001732"
                     /db_xref="InterPro:IPR008927"
                     /db_xref="InterPro:IPR013328"
                     /db_xref="InterPro:IPR014026"
                     /db_xref="InterPro:IPR014027"
                     /db_xref="InterPro:IPR014028"
                     /db_xref="InterPro:IPR016040"
                     /db_xref="InterPro:IPR017476"
                     /db_xref="UniProtKB/TrEMBL:Q4K352"
                     /translation="MKIAIAGSGYVGLSLAVLLAQHHEVKVIDVIKDKVESINNRKSP
                     IKDEAIEKYLVENELNLEASLDPAHVYKDVEYAIIATPTNYDVDLNQFDTSSVEAAIK
                     TCMEYNDTCTIVIKSTIPEGYTKEVREKFNTDRIIFSPEFLRESKALYDNLYPSRIVV
                     GTDLDDSELTKRAWQFADLLKGGAIKEEVPILVVAFNEAEVAKLFSNTYLATRVAYFN
                     EIDTYSEVKGLNPKTIIDIVCYDPRIGSYYNNPSFGYGGYCLPKDTKQLKASFRDVPE
                     NLITAVVQSNKTRKDYIAGAILAKQPSVVGIYRLIMKSDSDNFRSSAVKGVMERLDNY
                     GKEIVIYEPTIECDTFMGYRVIKSLDEFKNISDIVVANRMNDDLRDIQEKLYTRDLFG
                     RE"
     misc_feature    5752..6288
                     /gene="ugd"
                     /locus_tag="SPC03_0009"
                     /note="HMMPfam hit to PF03721, UDP-glucose/GDP-mannose
                     dehydrogenase, score 1.1e-42"
     misc_feature    5752..6198
                     /gene="ugd"
                     /locus_tag="SPC03_0009"
                     /note="HMMPfam hit to PF01210, NAD-dependent
                     glycerol-3-phosphate deh, score 0.00044"
     misc_feature    6337..6618
                     /gene="ugd"
                     /locus_tag="SPC03_0009"
                     /note="HMMPfam hit to PF00984, UDP-glucose/GDP-mannose
                     dehydrogenase, score 3.8e-33"
     misc_feature    6679..6915
                     /gene="ugd"
                     /locus_tag="SPC03_0009"
                     /note="HMMPfam hit to PF03720, UDP-glucose/GDP-mannose
                     dehydrogenase, score 0.00028"
     gene            6952..8205
                     /gene="wchE"
                     /locus_tag="SPC03_0010"
     CDS             6952..8205
                     /gene="wchE"
                     /locus_tag="SPC03_0010"
                     /codon_start=1
                     /transl_table=11
                     /product="serotype 3 capsule synthase"
                     /protein_id="CAI32747.2"
                     /db_xref="InterPro:IPR001173"
                     /db_xref="UniProtKB/TrEMBL:Q4K351"
                     /translation="MYTFILMLLDFFQNHDFHFFMLFFVFILIRWAVIYFHAVRYKSY
                     SCSVSDEKLFSSVIIPVVDEPLNLFESVLNRISRHKPSEIIVVINGPKNERLVKLCHD
                     FNEKLENNMTPIQCYYTPVPGKRNAIRVGLEHVDSQSDITVLVDSDTVWTPRTLSELL
                     KPFVCDKKIGGVTTRQKILDPERNLVTMFANLLEEIRAEGTMKAMSVTGKVGCLPGRT
                     IAFRTEILRECIHEFMNETFMGFHKEVSDDRSLTNLTLKKGYKTVMQDTSVVYTDAPT
                     SWKKFIRQQLRWAEGSQYNNLKMTPWMIRNAPLMFFIYFTDMILPMLLISFGVNIFLL
                     KILNITAIVYTASWWEIILYVLLGMIFSFGGRHFKAMSRMKWYYVFLIPVFIIVLSII
                     MCPIRLLGLMRCSDDLGWGTRNLTE"
     misc_feature    7117..7641
                     /gene="wchE"
                     /locus_tag="SPC03_0010"
                     /note="HMMPfam hit to PF00535, Glycosyl transferase
                     family, score 7.8e-17"
     gene            8503..9423
                     /gene="galU"
                     /locus_tag="SPC03_0011"
     CDS             8503..9423
                     /gene="galU"
                     /locus_tag="SPC03_0011"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosephosphate uridylyltransferase"
                     /protein_id="CAI32748.2"
                     /db_xref="GOA:Q4K350"
                     /db_xref="InterPro:IPR005771"
                     /db_xref="InterPro:IPR005835"
                     /db_xref="UniProtKB/TrEMBL:Q4K350"
                     /translation="MKKVKKAVIPAAGLGTRFLPATKALAKEMLPIVDRPTIHFVIEE
                     ALRSGIEDILVVTEKSKRSIEDYFDSTFELEYSLRKQGKMELLKSVNESTDIKVHFVR
                     QSSPRGLGDAVLQAKSFVGDDPFVVMLGDDLMDITDSTAVPLTRQLMDDYNATQASTI
                     AVMPVRYEDVSSYGVISPRLESSNGLYSVDAFVEKPKPEEAPSNLAIIGRYLLTPEIF
                     SILETQKPGAGNEIQLTDAIDTLNKTQSVFAREFVGKRYDVGDKFNFMKTSIDYALQH
                     PQIKESLKNYVIALGKQLEKLDDCSSSGHL"
     misc_feature    8518..9327
                     /gene="galU"
                     /locus_tag="SPC03_0011"
                     /note="HMMPfam hit to PF00483, Nucleotidyl transferase,
                     score 2.8e-21"
     gene            9420..>10337
                     /gene="pgm"
                     /locus_tag="SPC03_0012"
     CDS             9420..>10337
                     /gene="pgm"
                     /locus_tag="SPC03_0012"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="CAI32749.2"
                     /db_xref="GOA:Q4K349"
                     /db_xref="InterPro:IPR005844"
                     /db_xref="InterPro:IPR005845"
                     /db_xref="InterPro:IPR016055"
                     /db_xref="UniProtKB/TrEMBL:Q4K349"
                     /translation="MNCIESYQKWLNVPDLPAYLKDELLSMDDKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLVESKGETAKKAGVAIAYDSRHFSPEFAFE
                     SAQVLAAHGIKSYVFESLRPTPELSFAVRHLGAFAGIMVTASYTPAPFNGYKVYGSDG
                     GQMLPADADALTDYIRAIDNPFAVALADLEEAKSTGLIEVIGETLDAAYLEEVKSVNI
                     NQDLIDQYGRDMKIVYTPLHGTGEMLARRALAQAGFESVQVVEAQAKPAPDFSTVASP
                     NPESQAAFALAEELGRQVDADVLVATTLAA"
     misc_feature    9534..9965
                     /gene="pgm"
                     /locus_tag="SPC03_0012"
                     /note="HMMPfam hit to PF02878,
                     Phosphoglucomutase/phosphomannomutase, alph, score
                     4.8e-50"
ORIGIN      
        1 gaccgtcgct tcctagttgt ggctaacttg tccaatgaag agcaagactt gacagtagaa
       61 ggaaaagtca aatctgtctt gattgaaaac accctagctc aagaagtctt tgaaaaacaa
      121 atcttagttc catgggatgc tttctgtgtg gaattactat aaatattttt tgcagaaaaa
      181 tttaaaattg aaatcgtata aaaacaaggg aggactgtat aaaagacaga aatcctttgt
      241 tttttataac aaaagtttat aaaccttcat tcttgaaatt caattaactt tacaaattcc
      301 cactattaag gagaaagaag atgaacataa agaagcgtgt ccttggtgca ggtctgactt
      361 ttgcatctgc tttgctttta gctgcttgcg gccaatcagg ttcagataca aaaacttact
      421 catcaacctt tagtggaaat ccaactacat ttaactatct attagactat tacgctgata
      481 atatagtcaa ttgaaacaag agcaggacaa aagagcctcg taaaaggtat tgcaacttgg
      541 taataccttt ttgatgtgtt ttttgatatg agcccatgtt ttctcaatag gattgtactc
      601 aggtgagtag ggaggaagag gtaaaagttt atgcccaaac tcttcgcata aaagttctag
      661 cttccccatt ctatggaatc ttgcattatc cataataata accgatggtg tggttaatgt
      721 tggtaagaaa aacttctgaa accatacttc aaaaaagtcg ctcgtcatca tctcttcgta
      781 agtcattgga gcgattaact caccattcat ttgttagacc tgcaaccaaa gaaatcctct
      841 gatatcttct tccagatact ttgcctcttc ttaactgacc ttttaaagag cgaccatgtt
      901 ctcgataaaa ataagtatcg aatcctgttt catcaatcta aacaggtgct aggtgcttta
      961 aactattaaa attcttaaga aataaggcta cttttctggg tcttgttcat agtaggtgtg
     1021 gttctttttt tcgagtgtag cccatagctt tgagcgcata gtggatggta gttggatgac
     1081 agccaaattc agaagctatt tcagtcaaat aagtatctgg attgtcagta agatagtttt
     1141 taagtctatc tctatcaact tttcttggtt ttgttccttt tacttggtgg tttagctctc
     1201 ctgttttttc ttttagcttt aaccagccat aaatggtatt acgtgagatt tggaaaacgt
     1261 gtgatgcttc tgttatacta cctattcgct cacaataaga gagaactttt ttacgaaaat
     1321 ctattgaata tgccataaga agattatacc acattgtgta ctatattaga ttgaaactag
     1381 agtagtacac ctctgcttct aaaatattgt tagaaatcga tttgactgtc ctgatcgatt
     1441 tgtcctgttc ttatttcatt ttgctatatt tttggttcgc gggaagtcta ctaagatact
     1501 taaagataca gatagtgaaa ataaaggtgt agacattacc gttggtgttc tctatccttg
     1561 tcagctctgt gtcgctcttt gcagtacagc agtttgttgg actgaccaat cgtttaaatg
     1621 cgacttctaa ttactcagaa tattcgatca gtgtcgctgt tttagcagat agtgagatcg
     1681 aaaatgttac gcaactgacg agtgtgacag caccgactgg gactgataat gaaaatattc
     1741 aaaaattact agctgatatc aagtcagaat accgatttga cggtcgacca gagttcgtct
     1801 tacttggctg cttacaagag tctgattgca ggtgacacca agaccattgt cctaaatagt
     1861 gtctttgaaa atatcatcga gtcagagtat ccagactacg catcgaagat aaaaaatatt
     1921 tataccaagg gatttactaa aaaagtagaa gctcctaaga cgtctaagaa tcagtctttc
     1981 aatatctaag ttagtggaat tgacacctat ggtcctatta gttcggtgtc gcgatcagat
     2041 gttaatatcc tgatgactgt caatcgagat accaagaaaa tcctcttgac cacaacgccg
     2101 cgtgatgcct atgtaccaat tgcagatggt ggaaataatc aaaaagataa attgactcat
     2161 gcgggcattt atggagttga ttcgtccatt cacaccttag aaaatctcta tggagtggat
     2221 atcaattact atgtacgatt gaacttcact tcgtttttga aattgattga tttgttggat
     2281 ggaattgatg tttataatga tcaagaattt actgcccata cgaatggaaa gtattaccct
     2341 gaaggcaatg ttcatcttga ttcagaacag gctctcggtt ttgttcgtga gcgctactcc
     2401 ctagcagatg gcgatcgtga ccgcgggcgc aatcaacaaa aggtgattgt ggctatcctt
     2461 caaaaattaa cgtcaaccga agaactgaaa aattatagta cgatcattga tagcttgcaa
     2521 gattctatcc aaacaaatat gccgattgag actatgatag atttagtgaa tactcagttg
     2581 gaaagtggag ggaattataa agtaaattct caagatttaa aagggacagg tcggatggat
     2641 cttccttctt atgcaatgcc agacagtaac ctctatgtga tggaaataga tgatagtagt
     2701 ttagctgtag ttaaagcagc tatacaggat gtgatgaaag gtagatgaaa tgatagacat
     2761 ccattcgcat atcgtttttg atgtagatga cggtccaaag tcaatagagg atagcaaggc
     2821 acttttaaga gaagcttata atcaaggagt tcgaatgatt gtgtctactt cgcatcgtcg
     2881 aaaagggatg tttgaaactc cagaagaaaa aattgtaaca aattttatta aggttcgtga
     2941 aattgcaaaa gaagtagcag atgatttagt cattgcttat ggcgcagaga tatactatac
     3001 tctggatgct ctagaaaagc tagaaaaaaa gaaattccta cccttaatga tagtcgttat
     3061 gctttgattg agtttagcat gcatacttcc tatcgtgaga ttcatacgag attgagcaat
     3121 attttgatgt tgggaatcac gccagtaatt gctcatattg aacgttatga tgctttagag
     3181 aataacgaaa aacgtgttcg tgaactgatt gatatggggt gctatactca gatagatagt
     3241 tatcatgttt caaaacctaa gttctttggt gaaaaatata aattcatgaa aaagagagct
     3301 cggtattttt tggaacgtga tttagttcat gtagttgcaa gtgacatgca caatttagac
     3361 agtagacctc catatatgca acaggcatat gatatcattg ctaagaaata tagagcgaaa
     3421 aaagcgaaag aactttttgt agataatccc agaaaaatta taatggatca attaatttag
     3481 gagaaaatat gaaggaacaa aacactttgg aaatcgatgt attgcaacta ttcagagctt
     3541 tatggaaaag aaagttggtc attttattag tggcaattat aacttcttca gttgcttttg
     3601 cctacagtac ttttgttatc aaacctgagt ttactagtac gactcggatt tatgtagtta
     3661 accgtaatca gggagagaag tctggtttaa ccaatcaaga cttgcaggca ggatcatact
     3721 tggttaaaga ctatcgtgaa attatcctat cgcaggatgt tttggaggaa gttatttctg
     3781 atttgaaact agatttgacg ccaaaaggtt tggctaataa aattaaagta acagtaccag
     3841 ttgatacccg tattgtctct gtttcagtta atgatcgagt tcctgaagag gcaagccgta
     3901 tcgctaactc tttgagagaa gtagctgctc aaaaaattat cagtattact cgtgtttctg
     3961 atgtggcaac actggaggag gcaagaccgg cgatatcacc gtcttcgcca aatattaaac
     4021 gcaatacact aattggtttt ttggcagggg ggattggaac tagtgttata gttcttcttc
     4081 ttgaactttt ggacactcat gtgaaacgtc cggaagatat cgaagataca ctgcagatga
     4141 cacttttggg agttgtacca aacttgggta agttgaaata ggagagagga atgccgacat
     4201 tagaaatagc acaaaaaaaa ctggagttca ttaagaaggc agaagaatat tacaatgcct
     4261 tgtgtacaaa tatacagttg agcggagata aactaaaagt aatttccgtt acttctgtta
     4321 accctgggga aggaaaaaca actacttccg taaatatagc aaggtcgttt gcgcgtgcag
     4381 gctataaaac tcttttgatc gatggtgata ctcgaaattc agttatgtca ggatttttta
     4441 aatctcgtga aaaaattaca gagctaacag aatttttatc tgggacagct gatttatctc
     4501 acggtttatg tgatacaaat attgaaaatt tatttgtagt tcaatcggga tctgtatcac
     4561 caaaccctac agccttgtta caaagtaaaa attttgtggt tatggtaaag attttttcaa
     4621 aagaggtcag tatattgagt tggtggaaac gataaaatac ataatatttt attcctttgg
     4681 ttatcaaatt agcccctcct gaagctcccc aattgacggc ttgagctcca atcaaagggt
     4741 gtttgtccgc tttttgacag agggtaatcg ctagaggaca gcaaacggcc atagtagtga
     4801 aaaatccagc acctaaagca gacaaaaggg ttgccatcag gtataaaatc atgtagaggg
     4861 cgttagggtg ggtgcgtgtg cggtagagaa tgtgttgagc caaaacatca agagtaccgt
     4921 tagttgttgc aacgttataa aagagagaga cgctaaaaat ggtaaaaaag agtgaggttg
     4981 gccaaaaatg aagaagttct ttgggactta atcccatgag agtggttgcg atgaggtaag
     5041 aaaaagcaat agccagcagg ccaatattga ttttggtgcg gtaaccaatt ccaatggcta
     5101 gagcaatggc gctaatcatt attaaatgaa tcattgaatt gtcctttcag ttagaatata
     5161 gaaagaggat agattgaagt tcgagaatac tgggtgtctt ctgatgttaa gtggttgtgt
     5221 caaaaccatc ccaaatggca taaattgcgt ggaattggaa tgactcgtaa tactattgat
     5281 agagatggta ttacaagtca agacgttcgc tattttatct ttaactttaa gcttgatgtg
     5341 atgacctttt gccatagtgt tcgaggtcat tggtcagcag aaagtatgca ttggttattg
     5401 gatgtggttt atcgtgaaga ccatcatcag accctggata aacgagctgc ttttaacctt
     5461 aatcttatcc gaaaaatgtg tttatatttt ctaaaagtga tggtattttc taaaaaagac
     5521 ctcagctatc gatgcaaaca acggtatatc tctgtccatt tggaagatta tttggaaaca
     5581 gaggttagga aagtaattag tttaacggga tatcttttca aagctgatac taaggcacaa
     5641 aaaaagtttg atattcccct tgacaataga taaaattatt atataattaa actattgctt
     5701 tttaaataaa gtgagaatat taataataca gagaaagagg actgtagtaa aatgaaaatt
     5761 gccattgcag gaagtggtta tgtaggactg tctttagcgg tgctactagc tcagcatcat
     5821 gaagttaagg tcattgatgt tataaaggat aaggtagagt cgataaacaa tagaaaatct
     5881 ccaattaagg atgaggcgat tgagaaatac ttagttgaaa acgagttgaa tcttgaagcc
     5941 tccttagatc ctgcacacgt ttataaagac gtggagtatg ctattattgc tactccgact
     6001 aattatgatg tagacttaaa tcagtttgat acatcttcag ttgaagctgc tatcaagact
     6061 tgtatggaat ataatgatac ttgtacaatc gtaatcaaaa gtactattcc tgaagggtat
     6121 actaaagaag tgagggaaaa gtttaataca gatcgtatta tcttttctcc agagtttcta
     6181 cgtgaatcca aagctttata tgataatttg tatccatcta gaattgttgt aggaactgat
     6241 ttggatgatt ctgagttaac aaaaagagca tggcagtttg cagatctact taaaggtgga
     6301 gctattaagg aagaggttcc gatactggtt gttgctttta atgaagcaga ggttgcaaaa
     6361 ttgtttagta acacttactt ggcaactcgc gtagcttatt ttaatgagat agatacatat
     6421 agcgaggtaa aagggcttaa tcccaagaca attattgata ttgtttgtta tgatcctaga
     6481 attggatcat actataataa ccctagcttt ggttacggag ggtattgctt accaaaagac
     6541 acaaagcaat tgaaagcaag ttttagggat gttcctgaaa atctgattac agctgtggtg
     6601 caatctaata aaacaagaaa agattatata gctggagcta ttctagctaa acaacctagt
     6661 gttgtaggta tttatagatt aattatgaaa tctgattctg ataattttcg ttctagtgct
     6721 gttaagggag ttatggaacg tttggacaat tatggtaaag aaattgttat ttacgaacct
     6781 actattgagt gtgatacttt tatgggatac agagtaatta aatctttaga tgaatttaag
     6841 aatatttctg acattgttgt agcgaatcgt atgaacgatg atttaaggga tatacaagaa
     6901 aaactctata cacgcgattt atttggcaga gaataagggg aaataatttt tatgtataca
     6961 tttattttaa tgttattgga tttttttcag aatcatgatt ttcatttctt tatgttgttt
     7021 tttgtcttta ttcttattcg ttgggcggtt atatattttc atgctgtcag atataagtcc
     7081 tacagttgta gtgtaagtga tgagaagtta tttagttctg taattatccc tgtcgtggat
     7141 gaaccactta atctttttga aagtgtactg aatagaattt ccagacataa accatccgaa
     7201 attattgtgg ttattaacgg cccaaaaaac gagagacttg taaaactttg tcatgatttt
     7261 aatgaaaaat tagaaaataa tatgactcca attcaatgtt attacactcc tgttcctggc
     7321 aagagaaatg ctatccgcgt tgggctggag catgtggatt cgcagagtga tattacagtt
     7381 ctagtagata gtgatacagt atggacgcct agaaccttga gtgagttgct gaagcctttt
     7441 gtttgcgata aaaaaatagg tggggtaacg acaagacaaa aaattcttga ccctgagcgt
     7501 aatctcgtga caatgtttgc taacttgtta gaggaaatta gggcagaagg aactatgaaa
     7561 gcaatgagtg tgactggtaa agtagggtgc ttacctggtc gaacaattgc ttttagaaca
     7621 gagattctca gagagtgtat acatgagttt atgaatgaga ctttcatggg atttcataag
     7681 gaagtttctg atgatagaag tcttacaaat ttgactttaa aaaaaggcta taaaactgtt
     7741 atgcaggata cttctgttgt gtatacagat gctcctacaa gttggaaaaa gttcattaga
     7801 cagcaactaa ggtgggcaga aggttctcag tataacaatc taaagatgac tccttggatg
     7861 attagaaatg cccctcttat gttttttatt tattttacag atatgatttt acctatgcta
     7921 cttattagct ttggtgtgaa tatattcctg ttgaaaatat taaatataac tgcaattgtt
     7981 tatacagctt catggtggga aattatttta tatgttcttt tgggaatgat ttttagcttt
     8041 ggaggaagac actttaaagc tatgtctaga atgaagtggt attatgtatt tcttattcct
     8101 gtttttataa tcgttttgag tataattatg tgccctatta ggctattagg acttatgaga
     8161 tgttctgatg atttagggtg gggaactagg aatttaacag agtgagataa atagtagtgc
     8221 gtatatagag tatttactca gagtattaat tgatttttga aaaggaaaag tgtttttaaa
     8281 tgttaagaaa gaacttgaaa tatcagatta tgacacgagc tggaacaatt ttagctatct
     8341 tgtttttcat tatattaggg attattgttg aagttttgtt ttaaggctca ttgtcatctg
     8401 tagtggctca cttcagacgt aagggtcata ttttaatgtg aaaagagtgt taaaagatta
     8461 atcacttata ttattttaat agaaatagtg taaggaattg ttatgaaaaa agtaaaaaaa
     8521 gctgttattc ctgctgcagg gctgggcaca cgatttttgc ctgccactaa agctttggca
     8581 aaagaaatgc ttccaattgt agaccgcccc acaattcatt ttgtcattga agaagcttta
     8641 cgttcgggta ttgaagatat tctagtagtt actgaaaaga gtaaacgttc tattgaagat
     8701 tattttgatt caacttttga attggaatat agtcttagaa aacaaggaaa gatggaactt
     8761 cttaagtcag ttaatgaatc gactgatatt aaagtacatt tcgttcgtca aagttcacca
     8821 cgtggtcttg gtgacgctgt tctccaagcg aagtcttttg ttggtgacga tccctttgtt
     8881 gtaatgcttg gtgatgacct tatggatatc accgactcaa ctgctgtacc tttaacaaga
     8941 caattgatgg atgattacaa cgcaacacag gcttcaacta tcgcagtaat gcctgttaga
     9001 tatgaagatg tttcttctta tggtgtgatt tctcctagat tggaaagtag taatggcctc
     9061 tatagtgttg atgcttttgt agagaaacca aaaccagaag aagcgcctag caatttagct
     9121 attattggac gttatctact tactcctgag attttttcta tattagaaac ccaaaagcca
     9181 ggagcaggta atgaaattca attgacagat gctattgata cattgaataa gacacagagt
     9241 gtttttgcgc gtgaatttgt gggcaaacgt tatgatgttg gtgataagtt taattttatg
     9301 aaaacatcaa ttgattatgc tcttcaacat cctcagatta aagagagttt aaaaaattac
     9361 gttattgcac ttggtaagca attggagaag ctagatgact gttcgtcaag tggacaccta
     9421 tgaattgtat agaaagttat caaaaatggc taaatgtccc tgatcttcca gcttatttaa
     9481 aagatgaatt gctcagcatg gatgacaaaa caaaagaaga cgccttttac acaaaccttg
     9541 aattcggaac agctggtatg cgtggttata ttggtgctgg gacaaaccgt attaatatct
     9601 atgtggtgcg tcaagcaaca gaaggccttg ccaaattagt tgaatcaaaa ggcgaaacgg
     9661 ccaaaaaagc tggggttgct attgcctatg actcgcgaca tttttcacca gaattcgctt
     9721 ttgaatctgc ccaagtatta gcggcccatg gcattaaatc ttatgttttt gaaagcctac
     9781 gccctactcc tgagctgtct tttgctgttc gtcatctcgg agcatttgct ggtattatgg
     9841 taaccgccag ttatacccct gctcctttta atggttataa agtttacggt tctgatggtg
     9901 ggcaaatgct tccagctgat gctgatgctt taactgacta tattcgtgcg attgataacc
     9961 catttgctgt agcccttgct gacttagaag aagctaaatc aactggtctt attgaagtaa
    10021 ttggtgaaac tctcgatgct gcctaccttg aagaggttaa aagcgttaat atcaatcaag
    10081 atttgattga ccaatacggt cgcgatatga aaattgtcta cacacctctt catggtactg
    10141 gagaaatgct agcacgtcga gctttagcac aagctggttt cgaatctgtt caagttgtcg
    10201 aagctcaagc aaaaccagcc ccagacttct caacagttgc atcaccaaac cctgaaagtc
    10261 aagccgcctt tgccttagct gaagaactag ggcgtcaagt cgatgctgat gtattagtgg
    10321 cgactacttt agctgca
//

