LOCUS       JQ863076               14999 bp    DNA     linear   BCT 11-NOV-2012
DEFINITION  Vibrio parahaemolyticus strain 12968 serogroup O10 O serogroup
            genetic determinant region genomic sequence.
ACCESSION   JQ863076
VERSION     JQ863076.1  GI:410833256
KEYWORDS    .
SOURCE      Vibrio parahaemolyticus
  ORGANISM  Vibrio parahaemolyticus
            Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
            Vibrionaceae; Vibrio.
REFERENCE   1  (bases 1 to 14999)
  AUTHORS   Chen,M., Guo,D., Wong,H.C., Zhang,X., Liu,F., Chen,H., Chen,M.,
            Liu,B., Wang,L., Wu,F. and Feng,L.
  TITLE     Development of O-serogroup specific PCR assay for detection and
            identification of Vibrio parahaemolyticus
  JOURNAL   Int. J. Food Microbiol. 159 (2), 122-129 (2012)
   PUBMED   23072697
REFERENCE   2  (bases 1 to 14999)
  AUTHORS   Chen,M., Guo,D., Wong,H.C., Zhang,X., Liu,F., Chen,H., Chen,M.,
            Liu,B., Wang,L., Wu,F. and Feng,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2012) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..14999
                     /organism="Vibrio parahaemolyticus"
                     /mol_type="genomic DNA"
                     /strain="12968"
                     /db_xref="taxon:670"
                     /note="serogroup: O10"
     misc_feature    1..14999
                     /note="O serogroup genetic determinant region"
     gene            376..1086
                     /gene="kdkA"
     CDS             376..1086
                     /gene="kdkA"
                     /note="KdkA"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="AFV92955.1"
                     /db_xref="GI:410833257"
                     /translation="MIQQYRDSNQVIWFDEELIEDPSQPIFDAEYWQSTNKVTGSASG
                     RGTTWFIQLDTMQAALRHYRRGGLFGKLVKDNYWFSGWEQTRCAQEFQLLLTLINAGV
                     HVPRPIAARAVKSGLTYQADLLSERIPNARDLVSILQEKPLPEGMYQKIGQEIAKMHN
                     AGVNHTDLNIHNILIDDKDKVWIIDFDKCRKQEHCDWKKQNLERLLRSFKKELLKRQI
                     IWQASDFDHVQKSYTENT"
     gene            1179..2243
                     /gene="rmlB"
     CDS             1179..2243
                     /gene="rmlB"
                     /note="RmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-D-glucose 4,6-dehydratase"
                     /protein_id="AFV92956.1"
                     /db_xref="GI:410833258"
                     /translation="MKILVTGGAGFIGSAVVRHIIRDTQDSVVNLDKLTYAGNLESLV
                     DVADSDRYYFEQVDICDRTELDRVFSEHQPDMVMHLAAESHVDRSIDGPAAFIETNVM
                     GTYHLLEAARQYWSSLEEANKSAFRFHHISTDEVYGDLEGTDDLFTETTSYAPSSPYS
                     ASKASSDHLVRAWQRTYGLPTLVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGD
                     GMQIRDWLFVEDHARALYKVVTEGEIGETYNIGGHNEKANIEVVKTICALLEELRPDK
                     PAGVKSYESLITYVKDRPGHDVRYAIDATKIAQELNWTPEETFESGIRKTVEWYLNNP
                     QWWQRVLDGSYSLERLGAGE"
     gene            2243..3109
                     /gene="rmlA"
     CDS             2243..3109
                     /gene="rmlA"
                     /note="RmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AFV92957.1"
                     /db_xref="GI:410833259"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIREILIITTPEDQEGFQRLLGDGSEFGIELQYAIQPSPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIFYGQGFSPKLQQAAALTEGATVFGYKVKDPERFGVVEFDDEMRALSIEEK
                     PIEPKSNYAVTGLYFYDNRVVDIAKTIQPSHRGELEITSINQAYLEQGKLTVDVLGRG
                     FAWLDTGTHGSLLEAAHFVETIEKRQGYKVACLEEIAYRRGWLTREHIQAAGKALSKN
                     DYGRYLLELLEE"
     gene            3110..3529
                     /gene="wbtA"
     CDS             3110..3529
                     /gene="wbtA"
                     /note="WbtA"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-3,4-keto-hexulose isomerase"
                     /protein_id="AFV92958.1"
                     /db_xref="GI:410833260"
                     /translation="MSNIPLISFQKMGDERGSLISLEQHKNIPFDIKRIYYIFDTQSG
                     LARGFHAHYDLEQVAICMKGCVTFLIDDGTTKETVTLSSPDVGLHIKGLKWREMHDFS
                     EDCVLMVVASELYDEADYIRDYDTFLKEVAHANSCTQ"
     gene            3507..3977
                     /gene="wvdI"
     CDS             3507..3977
                     /gene="wvdI"
                     /note="WvdI"
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like protein"
                     /protein_id="AFV92959.1"
                     /db_xref="GI:410833261"
                     /translation="MRIHALSDVASSTIGEGTSIWQFAVVLAGAKIGRDCNICAHTFI
                     ENDVVLGDRVTVKCGVYLWDGIEIEDDVFIGPAAAFTNDKFPRSKVWPEAFPKTKILS
                     GASIGANATILPGITIGKNAMVGAGSVVTRSVPDNAVVVGNPAKIVRYVEKNNG"
     gene            3970..5073
                     /gene="wvaD"
     CDS             3970..5073
                     /gene="wvaD"
                     /note="WvaD"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase"
                     /protein_id="AFV92960.1"
                     /db_xref="GI:410833262"
                     /translation="MVNFLELKRLNAQYEDELKQACARVIESGWYILGNETEQFEQAF
                     ADYCGVKHCVGVANGLDALTLTLKAWRELGKLNKGDEVIVPGNTYIASVLAVTEADLV
                     PVFVEPDEQTHNISPEAIEAAITEKTKVILPVHLYGQLADMPAIMALATKYNLLVLED
                     SAQSHGATLAGKKSGAWGDASGFSFYPGKNLGALGDGGAITTSDDELAETLRALRNYG
                     SHVKYQNLYQGLNSRLDEIQSAMLSVKLNYLPSQTKARQELAEFYLREINNPNIILPE
                     VAEPEAHVWHLFVVRSSNRDALVKHLSEHGIQTQVHYPIAPHKQQAYKEFSHLSLPLT
                     EQLQNEVLSLPMSPYLSEQERNVVVDAVNSFEA"
     gene            5079..6008
                     /gene="wvcN"
     CDS             5079..6008
                     /gene="wvcN"
                     /note="WvcN"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFV92961.1"
                     /db_xref="GI:410833263"
                     /translation="MQDQVRVLEDVVYVFFCYKQSSYLDKTLPAALEQTAWPSQLLVL
                     DDCSPDDSHEKILSLLEFAPKGLNIEYRHNKQNIGLVSQINSLRGQYQNKLIIVQAGD
                     DIAMPNRIEETYETWLKNGKPSLIIGSYDKISEQGDVIEPFESIKQNKKPYTLENIIN
                     RKCAVDGCCAAFTSDVLNEFEPYNIDIINEDRINVLRAFLLNGIHHEHKKWIQYRLGG
                     ISTFAKETREEKYNNTVINAQRELQDLKMNLRDAQSKNANEVVSAIKKRMRTAEFMAS
                     LPQSNFALYSEAICYLVKTLDYRTVLKVLRRLK"
     gene            6005..6868
                     /gene="wvcO"
     CDS             6005..6868
                     /gene="wvcO"
                     /note="hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="WvcO"
                     /protein_id="AFV92962.1"
                     /db_xref="GI:410833264"
                     /translation="MSTIKNIKQSISMKRHFASTFRSFKKRSDKSTDSPKDFYRTYEK
                     CTVLGNGPSLKSDINNISKDTDVIAVNHFSESEHFTTLKPNKYILIDSYFWDEATHSS
                     LVEKRDKLFKDLNELVSWKMQLIIPDFADIAFIQGNIKNSNIAVKVLRAIPSNVTNIK
                     YGPIALSSGIVAPPAVNVLIFAVYVAILCEYDSVEIFGADMSFHNDVHVDQENNQLYM
                     VYKHFYGKEEKLPLLENPQKIKPFNMTDLMRLTFLTFYAHQLLNKWAELKGVKVVNRS
                     NFSMIDAYPRQ"
     gene            complement(6921..7601)
                     /gene="wvcP"
     CDS             complement(6921..7601)
                     /gene="wvcP"
                     /note="WvcP"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="AFV92963.1"
                     /db_xref="GI:410833265"
                     /translation="MSVHVFLPCRKGSERIPNKNTKDFAGVKGGLLKIKLDQLINITD
                     LDSIVVSSNDDVVLEFASTYKDSRIRIDERPEHLGSSSTSTDELIRYVPSIITTGHVL
                     WTHVTSPFLDESGYRAILKQYFASLSEGYDSLMTVKELRGFIWNKERPISYDRNIEKW
                     PRTQTIEPLYEIDSSVFLNSIENYKKLNDRIGLKPSMYIQDAESSIDIDWPEDFTFAE
                     SVWLQKNR"
     gene            complement(7604..8239)
                     /gene="wvcQ"
     CDS             complement(7604..8239)
                     /gene="wvcQ"
                     /note="WvcP"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="AFV92964.1"
                     /db_xref="GI:410833266"
                     /translation="MHPLFNGYDTVIFDCDGVILDSNNLKIDAMRNALRMAQVSELEI
                     DNCCNYFKANFGKSRFHHVSYFVEHFIINADETLYERILSFYSKQCEELYMEAQLTPN
                     FIAAITDCPAKLYVASGSAQDELREVFVKRKLAQYFTLILGSPTAKADNVATILKESS
                     GAALMIGDALSDFHAAKENNIDFLGYLPFSNVKEELESLSKSEGFNILRSW"
     gene            complement(8223..9833)
                     /gene="wvcR"
     CDS             complement(8223..9833)
                     /gene="wvcR"
                     /note="WvcR"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="AFV92965.1"
                     /db_xref="GI:410833267"
                     /translation="MQKILDCTLRDGGYYNHWDFSPEVVDAYLTAVAKAKIDYVELGL
                     RNYPKSVYSGPFAYTTEEFLNTLHLPKGPTYGVMVDAKTLLDANKPVEAAINDLFVPA
                     QESKVDLVRIAAHFNEAEQCESMIKAFKEMGYIVGLNLMQAGGKPSEVIAEKVQTVAK
                     ANPDVIYFADSLGNMDSAEVTRIAEIVKQNWDGDIGIHTHNNMGQAMSNTMTARSNGV
                     TWLDVTVTGMGRGAGNAQTENLLAELDGLDKYLPTAIYELVIRHFEPMQRRYGWGSSL
                     LYYLGAKNDIHPTYIQNMLSNPNYGTEEIVGAIEHLKKLEGTTSYNGDVLEEALTVGK
                     ISQPTESQSDLSGIFSGKEILLVTNTPNTATHRVAIETYIKTRKPIVIGINTKHEINT
                     ELFDYFAVSHNSKYLSESSHYSEVAKPVFLPKNRFDEDELSKFSNISMIDYSSTIEKD
                     TLVAHKNYCTLPYDNTAAYALCIAFIGQAQKISLVGFDGYDVTDRRQMEMNEIFSMVS
                     QQFGSTSIQALTPTTYPVKAGSIYAPAI"
     gene            complement(9938..11212)
                     /gene="kdtA"
     CDS             complement(9938..11212)
                     /gene="kdtA"
                     /note="KdtA"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="AFV92966.1"
                     /db_xref="GI:410833268"
                     /translation="MLVRIVYTLLLALASPLLLFGLYKSKPNKPKFGSRWREHFGITP
                     KLKSNDKPIWIHAVSVGESIAATPLIKALKEQNPEQSILVTTTTSTGAEQIAKLGNLV
                     EHRYMPIDFGFAIKGFLKAVQPKKMLIIETELWPNTLHNVHKAGIPITVVNARLSEKS
                     CHNYAKIQRLFNQLHPCLTQVLCQTDSDAERFERLGVNKEKLSVTGSIKFDIQISDHV
                     KQQGEALRAQLGKDRPVWIAASTHKGEDELVLDAHRQVLKSHPNALLVLVPRHPERFG
                     DVFTLCQHQGFNTVRRTSTHAVETNTQVYLGDTMGEMLTLMGAADICFMGGSLVGNKV
                     GGHNVLEPATLEKVIITGPSYFNFKEIVENLLAVDGILIVPDSTTLALRTTEIISQQD
                     KYLLRALHANKWASSNQGALNRTMSLLPKSDK"
     gene            complement(11542..12267)
                     /gene="waaF"
     CDS             complement(11542..12267)
                     /gene="waaF"
                     /note="WaaF"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose-LPS heptosyltransferase II"
                     /protein_id="AFV92967.1"
                     /db_xref="GI:410833269"
                     /translation="MKILVIGPAWVGDMVMSQSLYIVLKQRHPDSQIDVIAPGWCKPI
                     LERMPEIHQAIEMPIGHGEFNLLGRREIGKSLREKQYDHAYILPKSAKSALIPWFANI
                     PLRTGWKGEMRYGLLNDLRPNMKSFQYMVERYVALAYPKAEMVDSNSLGGLETLPRPR
                     LAINVAEQQATIKKFNLTQGQSIIGLCPGAEFGPAKKWPETHYAEVATQMARSGHQIW
                     LFGSQKDLETCNNIRALVPDAIP"
     gene            complement(12264..12989)
                     /gene="wvaH"
     CDS             complement(12264..12989)
                     /gene="wvaH"
                     /note="WvaH"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFV92968.1"
                     /db_xref="GI:410833270"
                     /translation="MKVFVVSLTRSLDRRERIEEKLKQQDITFEFFDAVDGSFDSFLH
                     SEKSRPTITLRRKGYQLKTSEVACFASHYELWVRCIELDEPIVILEDNVDPVEGLKQV
                     LEHTLPLTSNYDYIKLSATQKCAFTPIVPXXXXNHMLGGYSAGTCGTTAYIVTPKSAS
                     KFVMHAQEFIEPVDXYMEXPWRHGVQVYSVSPDLFTRAEIASTIGSKRKDKSKMTLAN
                     KIYAELFRTYESILKFLYWKNKA"
     gene            complement(12986..13972)
                     /gene="msbB"
     CDS             complement(12986..13972)
                     /gene="msbB"
                     /note="MsbB"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
                     acyltransferase"
                     /protein_id="AFV92969.1"
                     /db_xref="GI:410833271"
                     /translation="MTIKRDDFDPKAYNPEFKRHFLSPKYWGTWLGVILFLPLSLMPL
                     GLQKWLAKTIAKKLSKSHKGPAHRARVNFELCFPEKSFQEREALIEKTLYTAAMFFFR
                     FGLLSLRSPKWLQNRCQIRGLDILRQHIDNNENVILMVPHSWAIDVPAILLASMGKPV
                     SAMAKKQKNDVADWLMHKQRVQYGGRVYERSGGIKPFLKSIRDGYVGYYLPDQDHGPE
                     HSVFVDFFATRKATLPGLGKIAKLSRSKVLPLFASMNTEDGTFDIEILPAFELDKGEE
                     QDARTCNEAIEYFVGDKPEQYMWVLQLLHSQEDGNNYYNLFKPRYNSDWKVQ"
BASE COUNT     4196 a   3029 c   3375 g   4385 t
ORIGIN
        1 gcaggcaata ctggggtaag cgcattatca aagcgacagg ttattctata cagggattaa
       61 aagccgtttt taaacatgaa gctgctgttc gacaagagct cgctttgcta agcattgctg
      121 tgattttggc gtgcgtggtt gatgtcggga tgatagagcg catcttattg attggtgttg
      181 tggtattagt tctgattgtc gagctgatta actccgcgat agaagcagtg gtggatcgca
      241 ttggtgttga acgccatgaa ttaagtggcc gcgctaaaga tataggatcg gcagccgtta
      301 tggttgcgtt agcattcgca gcttttactt ggctatacat cttggccagt cgctacttag
      361 gctagaggtt acaacatgat tcagcagtat cgagatagca accaagtcat ttggtttgat
      421 gaggagctta ttgaagatcc tagtcagcct atttttgatg ccgagtattg gcaatccacc
      481 aacaaagtaa ctggcagtgc gtcagggcgt ggcacgactt ggtttattca gcttgatacg
      541 atgcaagccg ctctgcgtca ttatcgacgt ggtggcttgt ttggtaagtt agttaaagac
      601 aattattggt tttcaggttg ggagcaaacg cgttgtgcgc aagagttcca actattactt
      661 acactgatta atgctggcgt tcatgtgcca aggccaattg ctgcgagagc tgtgaaatct
      721 ggattgacat atcaggccga tttattgagt gagcgcattc ctaacgcaag agacctggtc
      781 agtatcttgc aggaaaaacc gttaccggaa ggaatgtatc aaaaaatagg gcaagaaatt
      841 gccaaaatgc acaacgctgg cgttaatcac acagacctga atatccacaa catcttgatc
      901 gatgacaaag ataaagtgtg gattattgat tttgataagt gccgcaaaca agagcactgc
      961 gactggaaaa agcaaaactt agaaaggctt cttcgctcat tcaaaaaaga actgctcaaa
     1021 cgccaaatca tttggcaagc gtccgatttt gatcatgtac aaaaaagtta tactgaaaat
     1081 acgtaaagtg agctttttgt cagttattcg tgagacaatc caatagcgca tttcggtaca
     1141 cccactaaaa gtacccatta aagtaggtaa catttgcaat gaaaatacta gtaacaggcg
     1201 gggcaggctt cattggctca gctgtcgttc gacacattat tcgtgacacc caagatagcg
     1261 tcgtgaattt agacaagctc acctacgcag gaaacttgga gtcgttggtg gatgtcgcgg
     1321 acagtgaccg ttattacttc gagcaagtgg atatctgtga ccgaactgaa ctagaccgcg
     1381 ttttttcaga gcatcaacct gatatggtga tgcatttggc cgctgagtct catgtcgatc
     1441 gctccattga tggtcctgct gcttttattg agaccaatgt gatgggcact taccatttgc
     1501 ttgaagcggc ccgtcaatat tggtcaagcc ttgaggaagc gaacaaatcg gcgtttcgtt
     1561 ttcatcatat ttctacggat gaagtgtatg gcgatttaga gggtacggat gatttattca
     1621 ccgaaaccac ttcatacgct ccaagcagcc catactcggc gtctaaagcg tcgagcgatc
     1681 accttgtacg cgcttggcag cgtacttatg gtctcccaac actcgtaacc aattgctcaa
     1741 acaactatgg tccgtaccac ttcccagaga agttgatccc actgatgatt ctgaatgccc
     1801 tcgatggcaa gcctttacct gtgtatggtg atggcatgca aatccgagat tggctgtttg
     1861 tggaagacca tgctcgcgcg ctttacaaag tcgtgacgga aggtgaaatc ggtgagacct
     1921 acaacatcgg tggccataac gaaaaagcaa acatcgaagt agtgaaaacc atttgtgctt
     1981 tgcttgaaga gctgcgacca gacaagccgg caggtgttaa gtcttatgaa tctttgatca
     2041 cttatgtcaa agatcgccca ggtcatgatg tgcgctacgc aattgatgcc accaagattg
     2101 ctcaagagct taactggacg ccagaagaaa cctttgaaag cggcattcgc aaaaccgtgg
     2161 aatggtacct caataatccg caatggtggc aacgcgtgct tgatggttca tacagtttag
     2221 agcgattagg agcgggcgaa taatgaaagg cattatttta gcaggtggct cgggcactcg
     2281 cctttaccct attacacgcg gtgtctctaa gcagttactg ccggtttatg acaaaccaat
     2341 gatttactac ccgttgtcgg tactcatgct tgctggcatt cgtgaaatct tgatcatcac
     2401 cacgccagaa gaccaggaag gatttcagcg cctattgggt gatggctcag agtttggcat
     2461 tgagttgcaa tacgcgattc aaccaagtcc agatggctta gctcaagcct tcatcatcgg
     2521 cgaagagttc attggcgatg actcagtatg tttagtgctg ggtgacaaca tcttttatgg
     2581 gcaaggtttt tcgccgaagc tacagcaagc cgcagcatta acagaaggtg cgacggtctt
     2641 tggctataaa gtgaaagatc ctgagcgctt tggtgtggtg gaatttgacg acgagatgcg
     2701 agcactatcg attgaagaga agccaatcga gccaaaatcc aattacgccg tgacagggct
     2761 gtatttctac gataatcgag tcgtggacat tgccaaaact attcaaccgt cccatcgtgg
     2821 cgagctagaa attacgagta ttaaccaagc ttacttagag caagggaagt tgacggttga
     2881 cgtactaggg cgcggctttg cgtggttaga cacgggaaca cacggcagct tgcttgaagc
     2941 tgcgcatttt gttgaaacga ttgagaaacg ccagggttat aaagtggctt gcttagaaga
     3001 gattgcatat cgccgagggt ggttaacgcg agagcacatt caagccgcag gaaaagcctt
     3061 atctaaaaac gattatggcc gctatcttct agagttattg gaagaataaa tgtcgaatat
     3121 accgctaata tcttttcaga aaatgggtga tgagcgaggc tcattaatct cgttagagca
     3181 acataaaaac attccttttg atatcaaacg tatttactac atctttgata cacaaagcgg
     3241 tttagcgaga gggtttcatg cacactacga cttagagcaa gtcgctattt gtatgaaagg
     3301 ttgcgtgact ttcttaattg atgatggcac cacgaaagag acagtgacgc tttcttctcc
     3361 cgatgtagga ttgcatatca aagggttaaa atggcgtgag atgcacgact tttctgaaga
     3421 ttgtgtgcta atggttgtcg ctagtgaatt gtatgatgaa gccgattata tccgtgacta
     3481 tgacacattt cttaaagagg tagcccatgc gaattcatgc actcagtgat gttgcttcat
     3541 cgacgattgg tgagggaacg tctatttggc aatttgccgt ggtccttgct ggggctaaaa
     3601 ttggtcgtga ctgtaatatc tgtgcccata ccttcatcga gaatgatgtg gtcctaggag
     3661 atagagtcac tgtaaagtgc ggcgtttatc tttgggatgg cattgaaatt gaagatgatg
     3721 tgttcattgg cccggcagct gctttcacga acgacaagtt cccacgttca aaagtatggc
     3781 cagaggcatt tccgaaaaca aaaatcctgt caggcgcctc catcggcgcc aatgccacga
     3841 ttttgccagg cataaccatt ggaaaaaatg cgatggttgg agcgggtagt gtggtcaccc
     3901 gttcagtgcc ggacaatgcg gttgtggttg ggaatccagc caagatagta cgttatgtag
     3961 agaaaaataa tggttaattt tttagagctt aagcgtctaa atgcgcagta tgaagacgaa
     4021 ttaaaacaag cttgtgcacg cgtgattgaa tctggctggt acattcttgg taatgaaacg
     4081 gaacagtttg aacaagcatt tgctgactac tgtggagtta aacactgtgt aggcgttgcg
     4141 aatggcttag atgctttaac actgacgtta aaggcttggc gagagctagg caaacttaac
     4201 aaaggtgatg aagttatcgt accgggcaat acctatatcg catcggtctt ggcggtcacc
     4261 gaagcggact tggtaccggt gtttgtggag ccggatgagc agacgcacaa tatcagtcct
     4321 gaagcgattg aagcggcgat tacagaaaag accaaagtga ttttgccggt gcatttgtat
     4381 ggtcagttag ccgatatgcc agcgattatg gcgttggcga caaaatacaa ccttcttgtg
     4441 ctggaagaca gcgctcagtc tcatggtgcg acacttgctg gtaagaagtc tggagcatgg
     4501 ggggatgcct ctggttttag cttctatccg ggtaaaaacc taggtgcgtt aggtgatggt
     4561 ggcgctatta cgactagcga tgatgaactg gcagaaacct tgcgtgccct tcgtaactac
     4621 ggctctcatg ttaaatacca gaatctttat caaggtttga acagtcgttt ggatgaaatt
     4681 cagtcggcga tgctgtcagt gaaactgaac taccttccat cgcagacgaa agcgcgtcag
     4741 gagctggcgg aattttactt acgtgaaatc aacaacccga atattatctt accggaagtg
     4801 gctgagcctg aagcccatgt atggcatttg tttgtggtgc gcagttcaaa ccgagatgca
     4861 ttggtgaagc atttatcaga gcatggtatt cagacacaag tacattaccc gatagcgccg
     4921 cataaacagc aggcttataa agagtttagt catctgtcat tgccgctgac ggagcagttg
     4981 caaaatgagg tgctgtcatt gccgatgtcg ccttacttga gtgagcaaga gagaaatgtt
     5041 gtggttgatg cagtgaactc ttttgaggcc taagagttat gcaagatcag gttcgagtat
     5101 tggaagatgt tgtctacgtc ttcttttgct ataaacaatc aagttatctc gataaaacgc
     5161 ttccggcagc attggagcaa acagcatggc cgagccagct gttagtattg gacgattgct
     5221 ctccagatga tagtcatgaa aagattctat cgttgttaga atttgcgcct aaaggattaa
     5281 atatcgaata tcgccacaat aagcaaaata ttggtttagt tagccaaatc aactccttac
     5341 gggggcagta ccaaaataag cttattattg tacaagctgg cgatgatata gcgatgccga
     5401 accgaattga ggaaacttat gaaacttggt tgaaaaatgg taaaccaagc ttaattattg
     5461 gtagttacga caaaatctct gagcaaggtg atgtcattga gccatttgaa tcgatcaagc
     5521 aaaataaaaa accttacaca ctcgaaaata ttattaatcg aaagtgtgca gtagatggct
     5581 gttgtgctgc ttttacctct gatgtattga atgagtttga gccctacaac atagacatca
     5641 tcaatgaaga ccggatcaat gttctccgag cctttttgtt aaatggtatt catcatgaac
     5701 acaaaaagtg gattcaatat cggctaggtg gcatatcaac gttcgcaaaa gaaacacgcg
     5761 aagaaaaata taacaacaca gtgatcaatg ctcagcgtga gctgcaagat cttaaaatga
     5821 acttacgaga tgcgcagagt aaaaatgcca atgaggttgt gagtgcgatt aaaaaacgga
     5881 tgcgtactgc tgagtttatg gcatcacttc cacaatcaaa ttttgctttg tacagcgaag
     5941 cgatttgtta tcttgttaaa acattggatt atcgaacggt tttaaaagtt ttgcgtaggt
     6001 taaaatgagt acaattaaaa atataaagca atcgataagt atgaagaggc attttgcttc
     6061 aactttccgc tcattcaaaa aacgaagtga taaaagtact gactcaccta aagactttta
     6121 tcgtacatat gagaaatgta ctgttttagg taatggacca tccttaaaat cagatattaa
     6181 taatatcagt aaagatactg atgtgattgc tgtgaatcac ttttcggagt ctgagcactt
     6241 tacgactttg aagcccaata aatatatttt gattgatagc tatttctggg atgaagccac
     6301 tcatagttcg ttggtggaaa aaagagataa attgtttaaa gatctaaacg aacttgtgtc
     6361 ttggaaaatg caattgatta ttcctgactt tgctgacata gcgttcattc agggaaatat
     6421 caaaaacagc aacatagctg taaaagtgct tcgagcaatt cctagtaatg taaccaatat
     6481 taaatatggg ccaatagcat tatcttcagg tattgttgca cctccagccg taaatgttct
     6541 tatctttgct gtgtatgtgg caattttatg tgaatatgat tcagtggaaa tatttggggc
     6601 agatatgagc ttccataatg atgtccatgt cgatcaagaa aataaccagc tttatatggt
     6661 ctacaagcat ttctatggaa aagaagagaa gttgccactt ctagaaaatc ctcaaaagat
     6721 caaacctttt aacatgacgg acttgatgcg attaactttt ctgacgtttt atgcacatca
     6781 actgctgaat aaatgggcag agctaaaagg ggtaaaagtc gtaaataggt ctaatttttc
     6841 tatgatcgat gcttaccctc ggcagtgaaa ttacaggtaa gtagacgcta caatggtaat
     6901 gcccacttac ctatgattat ttatctattt ttctgtaacc aaacggactc tgcgaaagta
     6961 aagtcttctg gccaatctat atcaattgag ctctctgcat cctgtatata catcgaaggc
     7021 tttaatccaa ttcggtcatt tagttttttg tagttttcta tgctatttaa aaagactgag
     7081 ctatcgattt catacagagg ttcaatcgtc tgagttctag gccatttttc tatattacga
     7141 tcgtagctaa ttggcctctc tttattccaa ataaaaccgc gcagctcttt taccgtcatt
     7201 aatgaatcat atccttcaga gagactcgcg aaatattgtt ttagaattgc acgataaccg
     7261 gattcatcga ggaaaggcga ggttacatga gtccaaagta cgtgacctgt agtaataatc
     7321 gaaggaacat atctaataag ctcatcagtg cttgttgaac ttgagcctaa gtgctctggg
     7381 cgttcatcta ttcgtatgcg agaatcttta tatgttgaag caaactctag aacaacatcg
     7441 tcgtttgaag atacgactat cgaatctagg tcagttatat tgattaactg atccaatttg
     7501 atttttagca acccaccttt tacaccagca aagtctttgg tatttttgtt tgggatacgt
     7561 tcactgcctt ttcggcatgg taaaaagaca tggacactca tgcttaccaa ctccttagaa
     7621 tattgaaacc ttcacttttt gataagcttt caagctcttc ttttacattt gaaaaaggaa
     7681 gatagccaag aaaatcgata ttattctctt tggctgcgtg aaagtctgat aatgcgtcac
     7741 cgatcattag agccgcgcct gaagactcct ttaggatggt tgcaacgtta tctgctttcg
     7801 cggtaggtga tcctagaatt aacgtaaaat attgtgccag ctttctttta acaaatactt
     7861 ctcgtaattc gtcttgagca gatccactcg ctacatagag tttggctggg caatctgtaa
     7921 ttgctgcaat aaaattaggc gttagttgag cttccatgta tagctcttca cactgctttg
     7981 agtagaagct tagtatacgt tcatacaatg tttcatctgc atttatgatg aaatgttcaa
     8041 caaagtaact cacatgatga aaacgcgatt tgccaaaatt agctttaaag taattgcaac
     8101 aattgtctat ttcgagctct gatacttggg ccatgcgtaa agcattacgc atggcatcaa
     8161 ttttgaggtt atttgagtct aggatcacac catcgcagtc aaaaataaca gtatcatagc
     8221 cattaaatag cgggtgcata aatagaccct gcttttactg gataagtggt aggggttaac
     8281 gcttgaattg atgtacttcc aaactgctga ctaaccatgc taaagatctc attcatttcc
     8341 atttggcgtc ggtcagtcac atcataacca tcaaaaccaa cgagagatat tttctgcgcc
     8401 tgacctataa aagcgataca taacgcatat gcggcggtat tgtcatacgg tagcgtacag
     8461 tagtttttat gagcgacgag cgtatccttc tctatagtgc tactgtaatc aatcatagaa
     8521 atattactaa atttgcttag ctcgtcttcg tcaaagcggt tctttggcaa aaacacgggc
     8581 ttggctacct ctgagtaatg gctgctttct gacaagtatt tagagttatg agatacagca
     8641 aagtaatcga aaagctcagt gttaatctca tgctttgtat tgatgccaat tacgataggc
     8701 tttcgagtct taatatatgt ctcgatcgcg actctatgtg ttgctgtatt gggcgtatta
     8761 gtgaccagta aaatttcttt tcctgaaaag atccctgata gatctgattg gctttcagtt
     8821 ggttggctta ttttccctac agtaagcgct tcttctaata catcaccatt atagcttgtc
     8881 gtaccttcta gcttttttaa atgctcgata gcgccaacaa tttcttctgt tccgtaatta
     8941 ggattagaaa gcatattttg aatataagtt gggtgaatat cgtttttggc tcccaagtaa
     9001 tatagaaggc ttgaacccca accgtatctt ctttgcattg gctcaaaatg gcgaataact
     9061 agttcatata ttgctgttgg caagtattta tctagtccat caagttcggc gagcaggttt
     9121 tcggtctggg cgttcccagc gcctcgtccc attccggtaa cggttacatc caaccatgta
     9181 acaccattcg aacgagctgt catcgtatta gacattgctt gacccatgtt gttatgggtg
     9241 tggatgccaa tgtcgccatc ccagttttgc tttacaattt cggcaatgcg agtgacttct
     9301 gcactatcca tattaccgag agagtcagca aaatagatga cgtcagggtt tgcttttgct
     9361 accgtctgta ctttttccgc tataacctcg gatggttttc cgcccgcttg catcagattt
     9421 aaaccaacga tataccccat ttctttgaaa gctttaatca tgctttcaca ctgctctgct
     9481 tcattgaaat gtgccgcgat acgaacgaga tcaactttac tttcttgtgc tggtacaaaa
     9541 agatcgttga tggctgcttc aacaggttta ttcgcatcta gcaacgtttt tgcgtcaacc
     9601 attacgccat atgtcggacc tttcggtagg tgtaacgtgt ttagaaactc ttcagtcgta
     9661 taagcaaatg gaccactgta aacagactta ggataattac gaagccctaa ttcaacataa
     9721 tcaattttag cttttgcaac tgcagtgagg taagcgtcaa cgacttctgg tgaaaagtcc
     9781 cagtggttat agtagccacc atcgcgaaga gtacagtcta aaattttttg cataatggat
     9841 attcttttta agttaaacag gtaatgtgct tagtacagta aaacatacaa gcttaatagt
     9901 aaaaagtgag gtatttatca aactaattat taagttatta cttatcactt ttggggagta
     9961 agctcattgt tcggtttaga gcgccttgat tggatgaggc ccatttatta gcatgcaaag
    10021 ctcggagtaa atatttatct tgttgagaaa tgatctctgt agtccttagg gcaagggtcg
    10081 ttgagtctgg cacaattaag ataccatcca cagctagtaa gttttcaaca atctccttaa
    10141 agttgaaata gctggggcca gtaatgatga ctttttctaa cgtagcaggc tctagcacat
    10201 tatgcccacc gaccttattt ccaactaggc ttccccccat aaagcagatg tccgctgcgc
    10261 ccatcaaagt tagcatctca cccattgtat cgcctagata tacttgagta ttagtctcta
    10321 ctgcatgcgt tgaggtacga cgcacagtgt taaagccttg atgttggcaa agcgtgaata
    10381 catcaccaaa tcgttctgga tggcgaggaa ccaaaacaag taaagcattt ggatgagatt
    10441 ttaaaacttg tctatgtgca tctaatacca gctcgtcttc gcctttgtgg gtgctcgctg
    10501 caatccatac tgggcgatct ttcccaagtt gcgctcgaag tgcttcgcct tgttgcttca
    10561 cgtgatctga aatttgaata tcaaatttaa tcgagcccgt gacagatagc ttctctttat
    10621 tgacgcctag gcgttcgaag cgctctgcat ctgaatctgt ttggcagagt acttgagtta
    10681 ggcatgggtg aagctggtta aaaagccgct gtatttttgc gtagttatga cacgattttt
    10741 cagagaggcg cgcattcacg acagttatag ggatacccgc tttgtgaaca ttgtgaagcg
    10801 tgtttggcca cagttcagtc tcaatgatca acattttttt tggttgtact gctttaagaa
    10861 aacctttgat agcgaaccca aagtcaattg gcatatagcg gtgttcaact aaattaccca
    10921 acttagcaat ctgttcagct cccgtactcg ttgttgtggt aactagaata gactgttcgg
    10981 ggttttgttc tttaagtgct ttaattaaag gtgttgctgc tatgctttcg ccaacagata
    11041 cggcatgtat ccaaataggt ttgtcgttgc tttttagctt gggagttatg ccaaagtgct
    11101 ctctccagcg agagccgaac ttaggcttat ttggtttgct cttgtacaaa ccaaataaaa
    11161 gcagcggtga cgctagggcg agtagtaagg tataaacaat acgaactaac atgagcttat
    11221 cgtaatggcg tcaagttttt ggatactatc tagcacttgt ttaggcgaaa gttctgtcaa
    11281 gcacttaagg tgtttgtatt ggcattcacg ctggaaacaa gggcgacaat caatatcggt
    11341 atgaacaatc tccacttttt ctgcgagngg gggggtgtan cttncggtgn aagttgagcc
    11401 atacacggca acaacattac aanccaaccg cagccgccac atgcattaag ccggaatcat
    11461 tcgcgacaac agtcttgcat gcagctagta gatcgaccgc ttcaattaag ctggntttgg
    11521 cctgctagaa catgaatgtt ctcatggaat tgcgtcgggn acaagtgcgc ggatattgtt
    11581 acaagtctct aaatcttttt gagaaccaaa caaccagatt tgatgcccag agcgagccat
    11641 ttgcgtcgca acttcagcgt agtgtgtttc gggccatttc ttggcaggac caaattctgc
    11701 tcctgggcac agtccaataa ttgattggcc ttgcgttaag ttgaactttt tgatcgtggc
    11761 ttgttgctca gccacgttga ttgcaaggcg tgggcgaggc aaggtttcaa gcccgccaag
    11821 agagttagag tccaccattt ctgcttttgg ataagctaac gctacatagc gctctaccat
    11881 gtactgaaaa gacttcatgt tagggcgtaa gtcattcaat aaaccatagc gcatttctcc
    11941 cttccagcca gtacgaagag gaatgttggc aaaccatggg atcagtgctg attttgctga
    12001 tttaggcaag atataggcgt ggtcatattg cttttcacgt aatgatttac caatttccct
    12061 gcggcctaat aagttaaatt caccatgacc gataggcatt tcaatcgctt ggtggatttc
    12121 tggcatacgc tcaagaatcg gtttacacca tcctggagcg ataacgtcaa tttggctatc
    12181 tggatgacgt tgctttaata cgatatataa cgattgcgac attaccatat cgccgaccca
    12241 cgcagggcca atgactaaaa tcttcatgct ttgtttttcc agtataggaa tttcaaaatt
    12301 gactcataag ttctaaaaag ttcagcgtat attttattcg caagagtcat tttggattta
    12361 tcttttcttt tactaccaat cgtcgaagca atctcagctc tagtaaaaag atctggagac
    12421 acgctgtaaa cttgaacacc atgtctccat ggttnttcca tgtagncatc gactggttca
    12481 ataaactctt gagcatgcat aacaaacttt gaggctgatt taggtgtgac gatataagcg
    12541 gtggtaccac aagtgccagc agagtagccc cctagcatat gattatnncn tncantaggt
    12601 acaattgggg tgaatgcaca cttctgagtg gcagacaact tgatgtagtc ataattagaa
    12661 gtcagtggaa gggtatgttc gagcacttgc ttcagccctt ctacggggtc gacgttgtct
    12721 tctaagatga cgatcggttc atcaagctca atacagcgaa cccacaactc gtaatggctt
    12781 gcaaagcagg cgacttcact ggtttttaat tgataccctt tgcggcgtag agtaatggtt
    12841 ggacgacttt tttctgaatg aagaaaactg tcaaaagagc catctacggc atcaaaaaat
    12901 tcaaacgtaa tgtcttgttg ttttaacttt tcttcaatac gttctcggcg atctagcgat
    12961 cgagtcaagc ttacgacaaa tactttcact gcactttcca gtcgctgtta tatcttggtt
    13021 taaacaaatt gtaatagtta ttaccgtctt cttgagagtg tagcaactgc agtacccaca
    13081 tatattgttc gggtttatca ccgacaaaat attcaatggc ttcattacag gtacgagcgt
    13141 cttgctcttc gcctttatcc agctcaaacg ccggtaaaat ctcaatatca aacgtaccat
    13201 cttcagtgtt catcgacgca aatagaggta gtacttttga gcgtgatagt ttcgcaattt
    13261 tgcctaaacc aggtagcgtt gctttacgtg tagcaaaaaa atcgacaaac acactatgct
    13321 ctgggccgtg atcttgatcg ggtagataat accctacata gccatctcga atggatttca
    13381 ggaaaggctt aatgccacct gagcgttcgt atacacgacc accgtattga actctttgct
    13441 tgtgcatcag ccagtcagca acatcattct tttgtttctt cgccattgca gacaccggct
    13501 ttcccattga agcgagcaag atagcaggca catcaattgc ccaagagtgg ggaaccatta
    13561 aaatcacatt ttcgttgtta tcaatgtgct gtcgtaaaat gtctaggccg cgaatttggc
    13621 aacggttctg aagccacttt ggtgagcgta aacttagtaa cccgaagcga aaaaagaaca
    13681 tggctgctgt gtagagtgtt ttttctatca aagcttcacg ttcttggaac gacttttccg
    13741 gaaaacacag ctcaaagtta accctcgctc ggtgagctgg acctttgtgg gatttagaca
    13801 gttttttagc tatggttttc gccaaccact tttgtaaacc cagtggcatt aatgataaag
    13861 gtaaaaataa gataacacct aaccacgtgc cccagtactt aggcgacaga aagtggcgct
    13921 taaactcagg gttatatgct ttagggtcga agtcatcacg tttaatggtc atggcgtgtc
    13981 tagttaccta ttcgtgtact ggcgaagtgg tttaaatgag aaaaagatag ttaggaatga
    14041 tacaggctat tgcacttgag taaaggtggc aggttatggt tctgccacct tgtggtgctt
    14101 aacggttttg gatagttaag tattcggcaa cgccttctgc aaccgtcttg aactcaacat
    14161 cacagcctgc ggcgcgcagt ttggtcagat cggcttgtgt gaattcttgg taagcgcctt
    14221 ttagatgttc agggaatggg atggtttcaa tttggccttt gccatgatgc ttgataaccg
    14281 cttttgctac ctcttcaaaa gattcagcac ggccagtacc gcagttgaaa atgcctgata
    14341 cgccgttttc taagaaccat aggttcactt tgcatacatc cccaacgtaa acgaagtcgc
    14401 gtttaaatga ttcgctgccc gcgaacaact taggattttc acctgcgttt agttggttgt
    14461 tcagatggaa cgcaacagat gccatgctgc ctttgtgatc ttcacgtggg ccatatacgt
    14521 tgaagtaacg gaagccggtg atttgagaaa gggtttcacc atgttcttca gcgtcttgcc
    14581 atagacgacg aacgtagtta tcaaactgct gtttcgagta accgtataca tttagtgcgc
    14641 cttcgtattc gcgttcttca acgaaagtat ctgtatcgcc gtaagtggct gcggaagagg
    14701 cgtataggaa tggaacttca cgatctaaac agtaatgaag cagctcttta gagtactcgt
    14761 aattgtttag catcatgtat ttaccatccc actctgtcgt tgcagagcaa gcgccttcgt
    14821 ggaaaattgc ttcgattgga ccgaaatcgt caccagccat aatttgggtc agaaagtcgt
    14881 cacgatccat gtaatctgtg atgtccagat caactaagtt cttaaacttt ttgccatttt
    14941 ttaggttgtc gacaaccagg atatcgttaa taccagcttc cgtttagtgc tttaaccaa
//
