LOCUS       JQ863082               14949 bp    DNA     linear   BCT 11-NOV-2012
DEFINITION  Vibrio parahaemolyticus strain 20582 serogroup O12 O serogroup
            genetic determinant region genomic sequence.
ACCESSION   JQ863082
VERSION     JQ863082.1  GI:410833351
KEYWORDS    .
SOURCE      Vibrio parahaemolyticus
  ORGANISM  Vibrio parahaemolyticus
            Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
            Vibrionaceae; Vibrio.
REFERENCE   1  (bases 1 to 14949)
  AUTHORS   Chen,M., Guo,D., Wong,H.C., Zhang,X., Liu,F., Chen,H., Chen,M.,
            Liu,B., Wang,L., Wu,F. and Feng,L.
  TITLE     Development of O-serogroup specific PCR assay for detection and
            identification of Vibrio parahaemolyticus
  JOURNAL   Int. J. Food Microbiol. 159 (2), 122-129 (2012)
   PUBMED   23072697
REFERENCE   2  (bases 1 to 14949)
  AUTHORS   Chen,M., Guo,D., Wong,H.C., Zhang,X., Liu,F., Chen,H., Chen,M.,
            Liu,B., Wang,L., Wu,F. and Feng,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2012) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..14949
                     /organism="Vibrio parahaemolyticus"
                     /mol_type="genomic DNA"
                     /strain="20582"
                     /db_xref="taxon:670"
                     /note="serogroup: O12"
     misc_feature    1..14949
                     /note="O serogroup genetic determinant region"
     gene            340..1050
                     /gene="kdkA"
     CDS             340..1050
                     /gene="kdkA"
                     /note="KdkA"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="AFV93044.1"
                     /db_xref="GI:410833352"
                     /translation="MIQQYRDSNQVIWFDEELIEDPSQPIFDAEYWQSTNKVTGSASG
                     RGTTWFVQLDTMQAALRHYRRGGLFGKLVKDNYWFSGWEQTRCAQEFQLLLTLINAGV
                     HVPRPIAARAVKSGLTYQADLLSERIPNARDLVSILREKPLPEGMYQKIGQEIAKMHN
                     AGVNHTDLNIHNILIDDKDKVWIIDFDKCRTQEHGDWKKQNLERLLRSFKKELLKRQI
                     IWQASDFDHVQKSHTENT"
     gene            1143..2207
                     /gene="rmlB"
     CDS             1143..2207
                     /gene="rmlB"
                     /note="RmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-D-glucose 4,6-dehydratase"
                     /protein_id="AFV93045.1"
                     /db_xref="GI:410833353"
                     /translation="MKILVTGGAGFIGSAVVRHIIRDTQDSVVNLDKLTYAGNLESLV
                     DVADSDRYYFEQVDICDRTELDRVFSEHQPDMVMHLAAESHVDRSIDGPAAFIETNVM
                     GTYHLLEAARQYWSSLEEANKSAFRFHHISTDEVYGDLEGTDDLFTETTSYAPSSPYS
                     ASKASSDHLVRAWQRTYGLPTLVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGD
                     GMQIRDWLFVEDHARALYKVVTEGEIGETYNIGGHNEKANIEVVKTICALLEELRPDK
                     PAGVKSYESLITYVKDRPGHDVRYAIDATKIAQELNWTPEETFESGIRKTVEWYLNNP
                     QWWQRVLDGSYSLERLGAGK"
     gene            2207..3073
                     /gene="rmlA"
     CDS             2207..3073
                     /gene="rmlA"
                     /note="RmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AFV93046.1"
                     /db_xref="GI:410833354"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIREILIITTPEDQEGFQRLLGDGSEFGIELQYAIQPSPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIFYGQGFSPKLQQAAALTEGATVFGYKVKDPERFGVVEFDDEMRALSIEEK
                     PIEPKSNYAVTGLYFYDNRVVDIAKTIQPSHRGELEITSINQAYLEQGKLTVDVLGRG
                     FAWLDTGTHGSLLEAAHFVETIEKRQGYKVACLEEIAYRRGWLTREHIQAAGKALSKN
                     DYGRYLLELLEE"
     gene            3074..3493
                     /gene="wbtA"
     CDS             3074..3493
                     /gene="wbtA"
                     /note="WbtA"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-3,4-keto-hexulose isomerase"
                     /protein_id="AFV93047.1"
                     /db_xref="GI:410833355"
                     /translation="MSNIPLISFQKMGDERGSLISLEQHKNIPFDIKRIYYIFDTQSG
                     LARGFHAHYDLEQVAICMKGCVTFLIDDGTTKETVTLSSPDVGLHIKGLKWREMHDFS
                     EDCVLMVVASELYDEADYIRDYDTFLKEVAHANSCTQ"
     gene            3471..3941
                     /gene="wvdI"
     CDS             3471..3941
                     /gene="wvdI"
                     /note="WvdI"
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like protein"
                     /protein_id="AFV93048.1"
                     /db_xref="GI:410833356"
                     /translation="MRIHALSDVSSSTIGEGTSIWQFAVVLAGAKIGRDCNICAHTFI
                     ENDVVLGDRVTVKCGVYLWDGIEIEDDVFIGPAAAFTNDKFPRSKVWPEAFPKTKILS
                     GASIGANATILPGITIGKNAMVGAGSVVTRSVPDNAVVVGNPAKIVRYVEKNNG"
     gene            3934..5037
                     /gene="wvaD"
     CDS             3934..5037
                     /gene="wvaD"
                     /note="WvaD"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase"
                     /protein_id="AFV93049.1"
                     /db_xref="GI:410833357"
                     /translation="MVNFLELKRLNAQYEDELKQACARVIESGWYILGNETEQFEQAF
                     ADYCGVKHCVGVANGLDALTLTLKAWRELGKLNKGDEVIVPGNTYIASVLAVTEADLV
                     PVFVEPDEQTHNISPEAIEAAITEKTKVILPVHLYGQLADMPAIMALATKYNLLVLED
                     SAQSHGATLAGKKSGAWGDASGFSFYPGKNLGALGDGGAITTSDDELAETLRALRNYG
                     SHVKYQNLYQGLNSRLDEIQSAMLSVKLNYLPAQTKARQELAEFYLREINNPNIILPE
                     VAEPEAHVWHLFVVRSSNRDALVKHLSEHGIQTQVHYPIAPHKQQAYKEFSHLSLPLT
                     EQLQNEVLSLPMSPYLSEQERNVVVDAVNSFEA"
     gene            5043..5972
                     /gene="wvcN"
     CDS             5043..5972
                     /gene="wvcN"
                     /note="WvcN"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFV93050.1"
                     /db_xref="GI:410833358"
                     /translation="MQDQVRVLEDVVYVFFCYKQSSYLDKTLPAALEQTAWPSQLLVL
                     DDCSPDDSHEKILSLLEFAPKGLNIEYRHNKQNIGLVSQINSLRGQYQNKLIIVQAGD
                     DIAMPNRVEETYETWLKNGKPSLIIGSYDKISEQGDVIEPFESIKQNKKPYTLENIIN
                     RKCAVDGCCAAFTSDVLNEFEPYNIDIINEDRINVLRAFLLNGIYHEHKKWIQYRLGG
                     ISTFAKETREEKYNNTVINAQRELQDLKMNLRDAQSKNANEAVSAIKKRMRSAEFMAS
                     LPQSNFALYSEAICYLVKTLDYRTVLKVLRRLK"
     gene            5969..6832
                     /gene="wvcO"
     CDS             5969..6832
                     /gene="wvcO"
                     /note="hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="WvcO"
                     /protein_id="AFV93051.1"
                     /db_xref="GI:410833359"
                     /translation="MSTIKNIKQSISMKRHFASTFRSFKKRSDKSTDSPKDFYRTYEK
                     CTVLGNGPSLKSDINNISKDTDVIAVNHFSESEHFTTLKPNKYILIDSYFWDEATHSS
                     LVEKRDKLFKVLNELVSWKMQLIIPDFADIAFIQGNIKNSNIAVKVLRAIPSNVTNIK
                     YGPIALSSGIVAPPAVNVLIFAVYVAILCEYDSVEIFGADMSFHNDVHVDQENNQLYM
                     VYKHFYGKEEKLPLLENPQKIKPFNMTDLMRLTFLTFYAHQLLNKWAELKGVKVVNRS
                     NFSMIDAYPRQ"
     gene            complement(6885..7565)
                     /gene="wvcP"
     CDS             complement(6885..7565)
                     /gene="wvcP"
                     /note="WvcP"
                     /codon_start=1
                     /transl_table=11
                     /product="CMP-N-acetylneuraminic acid synthetase"
                     /protein_id="AFV93052.1"
                     /db_xref="GI:410833360"
                     /translation="MSVHVFLPCRKGSERIPNKNTKDFAGIKGGLLKIKLDQLINITD
                     LDSIVVSSNDDDVLEFASTYKDSRIRIDERPEHLGSSSTSTDELIRYVPSIITTGHVL
                     WTHVTSPFLGESGYSAIIKQYNQALMEGYDSLMTVKELRGFIWNKDRPISYDRNIEKW
                     PRTQTIEPLYEIDSSVFLNSIENYKKLNDRIGLKPSMYIQDAESSIDIDWPEDFTFAE
                     SVWLQKNR"
     gene            complement(7568..8203)
                     /gene="wvcQ"
     CDS             complement(7568..8203)
                     /gene="wvcQ"
                     /note="WvcQ"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="AFV93053.1"
                     /db_xref="GI:410833361"
                     /translation="MHPLFNGYDTVIFDCDGVILDSNNLKIDAMRNALRMAQVSELEI
                     DNCCNYFKANFGKSRFHHVSYFVEHFILNTDEALYERILSFYSKQCEELYMEAQLTPN
                     FIAAITDCPAKLYVASGSAQDELREVFVKRKLAQYFTLILGSPTAKADNVATILKESS
                     GAALMIGDALSDFHAAKENNIDFLGYLPFSNVKEELESLSKSEGFNILRSW"
     gene            complement(8187..9797)
                     /gene="wvcR"
     CDS             complement(8187..9797)
                     /gene="wvcR"
                     /note="WvcR"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="AFV93054.1"
                     /db_xref="GI:410833362"
                     /translation="MQKILDCTLRDGGYYNHWDFSPEVVDAYLTAVAKAKIDYVELGL
                     RNYPKSVYSGPFAYTTEEFLNTLHLPKGPTYGVMVDAKTLLDANKPVEAAINDLFVPA
                     QESKVDLVRIAAHFNEAEQCESMIKAFKEMGYIVGLNLMQAGGKPSEVIAEKVQTVAK
                     ANPDVIYFADSLGNMDSAEVTRIAEIVKQNWDGDIGIHTHNNMGQAMSNTMTARSNGV
                     TWLDVTVTGMGRGAGNAQTENLLAELDGLDKYLPTAIYELVIRHFEPMQRRYGWGSSL
                     LYYLGAKNDIHPTYIQNMLSNPNYGTEEIVGAIEHLKKLEGTTSYNGDVLEEALTVGK
                     ISQPTESQSDLSGIFSGKEILLVTNTPNAATHRVAIETYIKTRKPIVIGINTKHEINT
                     ELFDYFAVSHNSKYLSESSHYSEVAKPVFLPKNRFDEDELSKFSNISMIDYSSTIEKD
                     TLTAHKNYCTLPYDNTAAYALCIAFIGQAQKISLVGFDGYDVTDRRQMEMNEIFSMIS
                     QQFGSTSIQALTPTTYPVKAGSIYAPAI"
     gene            complement(9902..11176)
                     /gene="kdtA"
     CDS             complement(9902..11176)
                     /gene="kdtA"
                     /note="KdtA"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="AFV93055.1"
                     /db_xref="GI:410833363"
                     /translation="MLVRIVYTLLLALASPLLLFGLYKSKPNKPKFGSRWREHFGITP
                     KLKSNDKPIWIHAVSVGESIAATPLIKALKEQNPEQSILVTTTTSTGAEQIAKLGNLV
                     EHRYMPIDFGFAIKGFLKAVQPKKMLIIETELWPNTLHNVHKAGIPITVVNARLSEKS
                     CHNYAKIQRLFNQLHPCLTQVLCQTDSDAERFERLGVNKEKLSVTGSIKFDIQISDHV
                     KQQGEALRAQLGKDRPVWIAASTHKGEDELVLDAHRQVLKSHPNALLVLVPRHPERFG
                     DVFTLCQHQGFNTVRRTSTHAVETNTQVYLGDTMGEMLTLMGAADICFMGGSLVGNKV
                     GGHNVLEPATLEKVIITGPSYFNFKEIVENLLAVDGILIVPDSTTLALRTTEIISQQD
                     KYLLRALHANKWASSNQGALNRTMSLLPKSDK"
     gene            complement(11170..12225)
                     /gene="waaF"
     CDS             complement(11170..12225)
                     /gene="waaF"
                     /note="WaaF"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose-LPS heptosyltransferase II"
                     /protein_id="AFV93056.1"
                     /db_xref="GI:410833364"
                     /translation="MKILVIGPAWVGDMVMSQSLYIVLKQRHPDSQIDVIAPGWCKPI
                     LERMPEIHQAIEMPIGHGEFNLLGRREIGKSLREKQYDHAYILPKSAKSALIPWFANI
                     PLRTGWKGEMRYGLLNDLRPNMKSFQYMVERYVALAYPKAEMVDSNSLGGLETLPRPR
                     LAINVAEQQATIKKFNLTQGQSIIGLCPGAEFGPAKKWPETHYAEVATQMARSGHQIW
                     LFGSQKDLETCNNIRALVPTQFHENIHVLAGQTSLIEAVDLLAACKTVVANDSGLMHV
                     AAAVGCNVVAVYGSTSPKYTPPLAEKVEIVHTDIDCRPCFQRECQYKHLKCLTELSPK
                     QVLDSIQKLDAITISSC"
     gene            complement(12222..12944)
                     /gene="wvaH"
     CDS             complement(12222..12944)
                     /gene="wvaH"
                     /note="WvaH"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFV93057.1"
                     /db_xref="GI:410833365"
                     /translation="MKVFVVSLTRSLDRRERIEEKLKQQDITFEFFDAVDGSFDSFLH
                     SEKSRPTITLRRKGYQLKTSEVACFASHYELWVRCIELDEPIVILEDNVDPVEGLKQV
                     LEHTLPLTSNYDYIKLSATQKCAFTPIVPIDDNHMLGGYSAGTCGTTAYLVTPKSASK
                     FVMHAQEFIEPVDDYMEKPWRHGVQVYSVSPDLFTRAEIASTIGGKRKDKSKMTLANK
                     IYAELFRTYESILKFLYWKNKA"
     gene            complement(12941..13927)
                     /gene="msbB"
     CDS             complement(12941..13927)
                     /gene="msbB"
                     /note="MsbB"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
                     acyltransferase"
                     /protein_id="AFV93058.1"
                     /db_xref="GI:410833366"
                     /translation="MTIKRDDFDPKAYNPEFKRHFLSPKYWGTWLGVILFLPLSLMPL
                     GLQKWLAKTIAKKLSKSHKGPAHRARVNFELCFPEKSFQEREALIEKTLYTAAMFFFR
                     FGLLSLRSPKWLQNRCQIRGLDILRQHIDNNENVILMVPHSWAIDVPAILLASMGMPV
                     SAMAKKQKNDVADWLMHKQRVQYGGRVYERSGGIKPFLKSIRDGYVGYYLPDQDHGPE
                     HSVFVDFFATRKATLPGLGKIAKLSRSKVLPLFASMNTEDGTFDIEILPAFELDKGEE
                     QDARTCNEAIEYFVGDKPEQYMWVLQLLHSQEDGNNYYNLFKPRYNSDWKVQ"
BASE COUNT     4195 a   3022 c   3357 g   4375 t
ORIGIN
        1 caggttattc tatacaggga ttaaaagccg tttttaaaca tgaagctgct gttcgacaag
       61 agctcgcttt gctaagcatt gctgtgattt tggcgtgcgt ggttgatgtc gggatgatag
      121 agcgcatctt attgattggt gttgtggtat tagttctgat tgtcgagctg attaactccg
      181 cgatagaagc agtggtggat cgcattggtg ttgaacgcca tgaattaagt ggccgcgcta
      241 aagatatagg atcggcagcc gttatggttg cgttagcatt cgcagctttt acttggctat
      301 acatcttggc cagtcgctac ttaggctaga ggttacaaca tgattcagca gtatcgagat
      361 agcaaccaag tcatttggtt tgatgaggag cttattgaag atcctagtca gcctattttt
      421 gatgccgagt attggcaatc caccaacaaa gtaactggca gtgcgtcagg gcgtggcacg
      481 acttggtttg ttcagcttga tacgatgcaa gccgctctgc gtcattatcg acgtggtggc
      541 ttgtttggta agttagttaa agacaattat tggttttcag gttgggagca aacgcgttgt
      601 gcgcaagagt tccaactatt acttacactg attaatgcag gtgttcatgt gccaaggcca
      661 attgctgcga gagctgtgaa atctggattg acatatcagg ccgatttatt gagtgagcgc
      721 attcctaacg caagagacct ggtcagtatc ttgcgggaaa aaccgttacc ggaaggaatg
      781 tatcaaaaaa tagggcaaga aattgccaaa atgcacaacg ctggcgttaa tcacacagac
      841 ctgaatatcc acaacatctt gatcgatgac aaagataaag tgtggattat tgattttgat
      901 aagtgccgca cacaagagca cggcgactgg aaaaagcaaa acttagaaag gcttcttcgc
      961 tcattcaaaa aagaactgct caaacgccaa atcatttggc aagcgtccga ttttgatcat
     1021 gtacaaaaaa gtcatactga aaatacgtaa agtgagcttt ttgtcagtta ttcgtgagac
     1081 aatccaatgg cgcatttcgg tacacccact aaaagtaccc attaaagtag gtaacatttg
     1141 caatgaaaat actagtaaca ggcggggcag gcttcattgg ctcagctgtc gttcgacaca
     1201 ttattcgtga cacccaagat agcgtcgtga atttagacaa gctcacctac gcaggaaact
     1261 tggagtcgtt ggtggatgtc gcggacagtg accgttatta cttcgagcaa gtggatatct
     1321 gtgaccgaac tgaactagac cgcgtgtttt cagagcatca acccgatatg gtgatgcatt
     1381 tagcggctga gtcgcatgtc gatcgctcta ttgatggtcc tgctgctttt attgagacca
     1441 acgtcatggg cacttaccat cttcttgaag cggcgcgtca atattggtca agccttgagg
     1501 aagcgaacaa atcggcgttt cgttttcatc atatttctac ggatgaagtg tatggcgatt
     1561 tagagggtac ggatgattta ttcaccgaaa ccacctcata cgctccaagc agcccatact
     1621 cggcgtctaa agcgtcgagc gatcaccttg tacgcgcttg gcagcgtact tatggtctcc
     1681 caacactcgt aaccaattgc tcaaacaact atggtccgta ccacttccca gaaaagttga
     1741 tcccactgat gattctgaat gccctcgatg gcaagccttt acctgtgtat ggtgatggca
     1801 tgcaaatccg agattggctg tttgtggaag accatgctcg cgcgctgtac aaagtcgtga
     1861 cggaaggtga aatcggtgag acctacaaca tcggtggcca taacgaaaaa gcaaacatcg
     1921 aagtagtgaa aaccatttgt gctttgcttg aagagctgcg accagacaag ccggcaggtg
     1981 ttaagtctta tgaatctttg atcacttatg tcaaagatcg cccaggtcat gatgtgcgct
     2041 acgcaattga tgcgaccaag attgctcaag agcttaactg gacgccagaa gaaacctttg
     2101 aaagcggcat tcgcaaaacc gtggaatggt acctcaataa tccgcaatgg tggcaacgcg
     2161 tgcttgatgg ttcatacagt ttagagcgat taggagcagg caaataatga aaggcattat
     2221 tttagcaggc ggctcgggca ctcgccttta ccctattaca cgcggtgtct ctaagcagtt
     2281 actgccggtt tatgacaaac caatgattta ctacccgttg tcggtactca tgcttgctgg
     2341 cattcgtgaa atcttgatca tcaccacgcc agaagaccag gaaggatttc agcgcctatt
     2401 gggtgatggc tcagagtttg gcattgagtt gcaatacgcg attcaaccaa gtccagatgg
     2461 cttagctcaa gccttcatca ttggtgaaga gttcattggc gatgactcag tatgtttggt
     2521 gctgggtgac aacatctttt atgggcaagg tttttcgccg aagctacagc aagccgcagc
     2581 attaacagaa ggtgcgacgg tctttgggta taaagtgaaa gatcctgagc gctttggtgt
     2641 ggtggaattt gacgacgaga tgcgagcact atcgattgaa gagaagccaa tcgagccaaa
     2701 atccaattac gccgtgacag ggctgtattt ctacgataat cgagtcgtgg acattgccaa
     2761 aactattcaa ccgtcccatc gtggcgagct agaaattacg agtattaacc aagcttactt
     2821 agagcaaggg aagttgacgg ttgacgtact agggcgcggc tttgcgtggt tagacacggg
     2881 aacacacggc agcttgcttg aagctgcgca ttttgttgaa acgattgaga aacgccaggg
     2941 ttataaagtg gcttgcttag aagagattgc atatcgccga gggtggttaa cgcgagagca
     3001 cattcaagcc gcaggaaaag ccttatctaa aaacgattat ggccgctatc ttctagagtt
     3061 attggaagaa taaatgtcga atataccgct aatatctttt cagaaaatgg gtgatgagcg
     3121 aggctcatta atctcgttag agcaacataa aaacattcct tttgatatca aacgtattta
     3181 ctacatcttt gatacacaaa gcggtttagc gagagggttt catgcacact acgacttaga
     3241 gcaagtcgct atttgtatga aaggttgcgt gactttctta attgatgatg gcaccacgaa
     3301 agagacagtg acgctttctt ctcccgatgt aggattgcat atcaaagggt taaaatggcg
     3361 tgagatgcac gacttttctg aagattgtgt gctaatggtt gtcgctagtg aattgtatga
     3421 tgaagccgat tatatccgtg actatgacac atttcttaaa gaggtagccc atgcgaattc
     3481 atgcactcag tgatgtttct tcatcgacga ttggtgaggg aacgtctatt tggcaatttg
     3541 ccgtggtcct tgctggggct aaaattggtc gtgactgtaa tatctgtgcc cataccttca
     3601 tcgagaatga tgtggtccta ggagatagag tcactgtaaa gtgcggcgtt tatctttggg
     3661 atggcattga aattgaagat gatgtgttca ttggcccggc agctgctttc acgaacgaca
     3721 agttcccacg ttcaaaagta tggccagagg catttccgaa aacaaaaatc ctgtcaggcg
     3781 cctccatcgg cgccaatgcc acgattttgc caggcataac cattggaaaa aatgcgatgg
     3841 ttggagcggg tagtgtggtc acccgttcag tgccggacaa tgcggttgtg gttgggaatc
     3901 cagccaagat agtacgttat gtagagaaaa ataatggtta attttttaga gcttaagcgt
     3961 ctaaatgcgc agtatgaaga cgaattaaaa caagcttgtg cacgcgtgat tgaatctggc
     4021 tggtacattc ttggtaatga aacggaacag tttgaacaag catttgctga ctactgtgga
     4081 gttaaacact gtgtaggcgt tgcgaatggc ttagatgctt taacactgac gttaaaggct
     4141 tggcgagagc taggcaaact taacaaaggt gatgaagtta tcgtaccggg caatacctat
     4201 atcgcatcgg tcttggcggt caccgaagcg gacttggtac cggtgtttgt ggagccggat
     4261 gagcagacgc acaatatcag tcctgaagcg attgaagcgg cgattacaga aaagaccaaa
     4321 gtgattttgc cggtgcattt gtatggtcag ttagccgata tgccagcgat tatggcgttg
     4381 gcgacaaaat acaaccttct tgtgctggaa gacagcgctc agtctcatgg tgcgacactt
     4441 gctggtaaga agtctggagc atggggggat gcctctggtt ttagcttcta tccgggtaaa
     4501 aacctaggtg cgttaggtga tggtggcgct attacgacta gcgatgatga actggcagaa
     4561 accttgcgcg cccttcgtaa ctacggctct catgttaaat accagaatct ttatcaaggt
     4621 ttgaacagtc gtttggatga aattcagtcg gcgatgctgt cagtgaaact gaactacctt
     4681 ccagcgcaga cgaaagcgcg tcaggagctg gcggaatttt acttacgtga aatcaacaac
     4741 ccgaatatta tcttaccgga agtagctgag cctgaagccc atgtatggca tttgtttgtg
     4801 gtgcgcagtt caaaccgaga tgcattggtg aagcatttat cagagcatgg tattcagaca
     4861 caagtgcatt acccgatagc gccgcataaa cagcaggctt ataaagagtt tagtcatctg
     4921 tcattgccgc tgacggagca gttgcaaaat gaggtgctgt cattaccgat gtcgccctac
     4981 ttgagtgagc aagagagaaa tgttgtggtt gatgcagtga actcttttga ggcctaagag
     5041 ttatgcaaga tcaggttcga gtattggaag atgttgtcta cgtcttcttt tgctataaac
     5101 aatcaagtta tctcgataaa acacttccgg cggcattgga gcaaacagca tggccgagcc
     5161 agctgttagt attggacgat tgctctccag atgatagtca tgaaaagatt ctatcgcttt
     5221 tagaatttgc gcctaaagga ttaaatatcg aatatcgcca caataagcaa aatattggtt
     5281 tagttagcca aatcaactcc ttgcgggggc agtaccaaaa taagcttatt attgtacaag
     5341 ctggcgatga tatagcgatg ccgaaccgag ttgaggaaac ttatgaaact tggttgaaaa
     5401 atggtaaacc aagcttaatt attggtagtt acgacaaaat ctctgagcaa ggtgatgtca
     5461 ttgagccatt tgaatcgatc aagcaaaata aaaaacctta cacactcgaa aatattatta
     5521 atcgaaagtg tgcagtagat ggctgttgtg ctgcttttac ctctgatgta ttgaatgagt
     5581 ttgagcccta caacatagac atcatcaatg aagaccggat caatgttctc cgagcctttt
     5641 tgttaaatgg tatttatcat gaacacaaaa agtggattca atatcggcta ggtggcatat
     5701 caacgttcgc aaaagaaaca cgcgaagaaa aatataacaa cacagtgatc aatgctcagc
     5761 gtgagctgca agatcttaaa atgaacttac gagatgcgca gagtaaaaat gccaatgaag
     5821 ctgtgagtgc gattaaaaaa cggatgcgtt ccgctgagtt tatggcatca cttccacaat
     5881 caaattttgc tttgtacagc gaagcgattt gttatcttgt taaaacattg gattatcgaa
     5941 cggttttaaa agttttgcgt aggttaaaat gagtacaatt aaaaatataa agcaatcgat
     6001 aagtatgaag aggcattttg cttcaacttt ccgctcattc aaaaaacgaa gtgataaaag
     6061 tactgactca cctaaagact tttatcgtac atatgagaaa tgtactgttt taggtaatgg
     6121 accatcctta aaatcagata ttaataatat cagtaaagat actgatgtga ttgctgtgaa
     6181 tcacttttcg gagtctgagc actttacgac tttgaagccc aataaatata ttttgattga
     6241 tagctatttc tgggatgaag ccactcatag ttcgttggtg gaaaaaagag ataaattgtt
     6301 taaagttcta aacgaacttg tgtcttggaa aatgcaattg attattcctg actttgctga
     6361 catagcgttc attcagggaa atatcaaaaa cagcaacata gctgtaaaag tgcttcgagc
     6421 aattcctagt aatgtaacca atattaaata tgggccaata gcattatctt caggtattgt
     6481 tgcacctcca gccgtaaatg ttcttatctt tgctgtgtat gtggcaattt tatgtgaata
     6541 tgattcagtg gaaatatttg gggcagatat gagcttccat aatgatgtcc atgtcgatca
     6601 agaaaataac cagctttata tggtctacaa gcatttctat ggaaaagaag agaagttgcc
     6661 acttctagaa aatcctcaaa agatcaaacc ttttaacatg acggacttga tgcgattaac
     6721 ttttctgacg ttttatgcac atcaactgct gaataaatgg gcagagctaa aaggggtaaa
     6781 agtcgtaaat aggtctaatt tttctatgat cgatgcttac cctcggcagt gaaattacag
     6841 gtaagtagac gctacaatgg taatgcccac ttacctatga ttatttatct atttttctgt
     6901 aaccaaacgg actctgcgaa agtaaagtct tctggccaat ctatatcaat tgagctctct
     6961 gcatcctgta tgtacatcga aggctttaat ccaattcggt catttagttt tttgtagttt
     7021 tctatgctat ttaaaaagac tgagctatcg atttcataca gcggttcaat cgtctgagtt
     7081 ctaggccatt tttctatatt acgatcgtag ctaattggcc tatctttatt ccaaataaaa
     7141 ccgcgtagct ctttgaccgt cattaatgaa tcatagcctt ccatgagtgc ttgattatac
     7201 tgtttgataa tagcgctata acctgactcg cctaagaatg gtgaggttac atgagtccaa
     7261 agtacgtgac ctgtcgtaat aatcgaagga acatatctaa taagctcatc agtgcttgtt
     7321 gaacttgatc ctaagtgctc cgggcgttca tctattcgta tgcgagaatc tttatatgtt
     7381 gaagcaaact ctagaacatc gtcatcattt gaagatacga ctatcgaatc taggtcagtt
     7441 atattgatta actgatccag tttgattttt agcaacccac cttttatacc agcaaagtct
     7501 ttggtatttt tgtttgggat acgttcactg ccttttcggc atggtaaaaa gacatggaca
     7561 ctcatgctta ccaactcctt agaatattga aaccttcact ttttgataag ctttcaagct
     7621 cttcttttac atttgaaaaa ggaagatagc caagaaaatc aatattattc tctttggctg
     7681 cgtgaaagtc tgataatgcg tcaccgatca ttagagccgc gcctgaagac tcctttagga
     7741 tggttgcaac gttatctgct ttcgcggtag gtgatcctag aattaacgta aaatattgtg
     7801 ccagctttct tttaacaaat acttctcgta attcgtcttg agcagatcca ctcgctacat
     7861 agagtttagc tgggcaatct gtaattgctg caataaaatt aggcgttagt tgagcttcca
     7921 tgtatagctc ttcacactgc tttgagtaga agcttagtat acgttcgtac aatgcttcat
     7981 ctgtatttag gatgaaatgt tcaacaaagt aactcacatg atgaaaacgc gatttgccaa
     8041 aattagcttt aaagtaattg caacaattgt ctatttcgag ctctgatact tgggccatgc
     8101 gtaaagcatt acgcatggca tcaattttga ggttatttga gtctaggatc acgccatcgc
     8161 agtcaaaaat aacagtatca tagccattaa atagcgggtg cataaataga ccctgctttt
     8221 actggataag tggttggggt taacgcttga attgatgtac ttccaaactg ctgactaatc
     8281 atgctaaaga tctcattcat ttccatttgg cgtcggtcag tcacatcata accatcaaaa
     8341 ccaacgagag atatcttctg cgcttgacct ataaaagcga tacataacgc atatgcagcg
     8401 gtattgtcat acggtagtgt acagtagttt ttatgggcgg tgagagtatc cttctctatg
     8461 gtgctactgt aatcaatcat agaaatatta ctaaatttac ttagctcgtc ttcgtcaaag
     8521 cggttctttg gcaaaaatac gggtttggct acttctgagt aatggctgct ttctgacaag
     8581 tatttagagt tatgagatac agcaaagtaa tcgaaaagct cagtgttaat ctcatgcttt
     8641 gtattgatgc caattacgat aggctttcga gtcttaatgt atgtctcgat cgcgactcta
     8701 tgtgttgcag cattgggcgt attagtgacc agtaaaattt cttttcctga aaagatccct
     8761 gatagatctg attggctttc agttggttgg cttattttcc ctacagtaag cgcttcttct
     8821 aagacatcac cattatagct tgtcgtacct tctagttttt ttaaatgctc gatagcgcca
     8881 acaatttctt ctgttccgta attaggatta gaaagcatat tttgaatata agttgggtga
     8941 atatcgtttt tggctcccaa gtaatataga aggcttgaac cccaaccgta tcttctttgc
     9001 attggctcaa aatggcgaat aactagttca tatattgctg ttggcaagta tttatctagt
     9061 ccatcaagtt cggcgagcag gttttcggtc tgggcgttcc cagcgcctcg tcccattccg
     9121 gtaacggtta catccaacca tgtaacacca ttcgaacgag ctgtcatcgt attagacatt
     9181 gcttgaccca tgttgttatg ggtgtggatg ccaatgtcgc catcccagtt ttgctttaca
     9241 atttcggcaa tgcgagtgac ttctgcacta tccatattac cgagagagtc agcaaaatag
     9301 atgacgtcag ggtttgcttt tgctaccgtc tgtacttttt ccgctataac ctcggatggt
     9361 tttccgcccg cttgcatcag atttaaacca acgatatacc ccatttcttt gaaagcttta
     9421 atcatgcttt cacactgctc tgcttcattg aaatgtgccg cgatacgaac gagatcaact
     9481 ttactttctt gtgctggtac aaaaagatcg ttgatggctg cttcaacagg tttattcgca
     9541 tctagcaacg tttttgcgtc aaccattacg ccatatgtcg gacctttcgg taggtgtaac
     9601 gtgtttagaa actcttcagt cgtataagca aatggaccac tgtaaacaga cttaggataa
     9661 ttacgaagcc ctaattcaac ataatcaatt ttagcttttg caactgcagt gaggtaagcg
     9721 tcaacgactt ctggtgaaaa gtcccagtgg ttatagtagc caccatcgcg aagagtacag
     9781 tctaaaattt tttgcataat ggatattctt tttaagttaa acaggtaatg tgcttagtac
     9841 agtaaaacat acaagcttaa tagtaaaaag tgaggtattt atcaaactaa ttattaagtt
     9901 attacttatc acttttgggg agtaagctca ttgttcggtt tagagcgcct tgattggatg
     9961 aggcccattt attagcatgc aaagctcgga gtaaatattt atcttgttga gaaatgatct
    10021 ctgtagtcct tagggcaagg gtcgttgagt ctggcacaat taagatacca tccacagcta
    10081 gtaagttttc aacaatctcc ttaaagttga aatagctggg gccagtaatg atgacttttt
    10141 ctaacgtagc aggctctagc acattatgcc caccgacctt atttccaact aggcttcccc
    10201 ccataaagca gatgtccgct gcgcccatca aagttagcat ctcacccatt gtatcgccta
    10261 gatatacttg agtattagtc tctactgcat gcgttgaagt acgacgcaca gtgttaaagc
    10321 cttgatgttg gcaaagcgtg aatacatcac caaatcgttc tggatggcga ggaaccaaaa
    10381 caagtaaagc atttggatga gattttaaaa cttgtctatg tgcatctaat accagctcgt
    10441 cttcgccttt gtgggtgctc gctgcaatcc atactgggcg atctttccca agttgcgctc
    10501 gaagtgcttc gccttgttgc ttcacgtgat ctgaaatttg aatatcaaat ttaatcgagc
    10561 ccgtgacaga tagcttctct ttattgacgc ctaggcgttc gaagcgctct gcatctgaat
    10621 ctgtttggca gagtacttga gttaggcatg ggtgaagctg gttaaaaagc cgctgtattt
    10681 ttgcgtagtt atgacacgat ttttcagaga ggcgcgcatt cacgacagtt atagggatac
    10741 ccgctttgtg aacattgtga agcgtgtttg gccacagttc agtctcaatg atcaacattt
    10801 tttttggttg tactgcttta agaaaacctt tgatagcgaa cccaaagtca attggcatat
    10861 agcggtgttc aactaaatta cccaacttag caatctgttc agctcccgta ctcgttgttg
    10921 tggtaactag aatagactgt tcggggtttt gttctttaag tgctttaatt aaaggtgttg
    10981 ctgctatgct ttcgccaaca gatacggcat gtatccaaat aggtttgtcg ttgcttttta
    11041 gcttgggagt tatgccaaag tgctctctcc agcgagagcc gaacttaggc ttatttggtt
    11101 tgctcttgta caaaccaaat aaaagcagcg gtgacgctag ggcgagtagt aaggtataaa
    11161 caatacgaac taacatgagc ttatcgtaat ggcgtcaagt ttttggatac tatctagcac
    11221 ttgtttaggc gaaagttctg tcaagcactt aaggtgtttg tattggcatt cacgctggaa
    11281 acaagggcga caatcaatat cggtatgaac aatctccact ttttctgcga gtgggggggt
    11341 gtacttcggt gaagttgagc catacacggc aacaacatta caaccaaccg cagccgccac
    11401 atgcattaag ccggaatcat tcgcgacaac agtcttgcat gcagctagta gatcgaccgc
    11461 ttcaattaag ctggtttggc ctgctagaac atgaatgttc tcatggaatt gcgtcgggac
    11521 aagtgcgcgg atattgttac aagtctctaa atctttttga gaaccaaaca accagatttg
    11581 atgcccagag cgagccattt gcgtcgcaac ttcagcgtag tgtgtttcgg gccatttctt
    11641 ggcaggacca aattctgctc ctgggcacag tccaataatt gattggcctt gcgttaagtt
    11701 gaactttttg atcgtggctt gttgctcagc cacgttgatt gcaaggcgtg ggcgaggcaa
    11761 ggtttcaagc ccgccaagag agttagagtc caccatttct gcttttggat aagctaacgc
    11821 tacatagcgc tctaccatgt actgaaaaga cttcatgtta gggcgtaagt cattcaataa
    11881 accatagcgc atttctccct tccagccagt acgaagagga atgttggcaa accatgggat
    11941 cagtgctgat tttgctgatt taggcaagat ataggcgtgg tcatattgct tttcacgtaa
    12001 tgatttacca atttccctgc ggcctaataa gttaaattca ccatgaccga taggcatttc
    12061 aatcgcttgg tggatttctg gcatacgctc aagaatcggt ttacaccatc ctggagcgat
    12121 aacgtcaatt tggctatctg gatgacgttg ctttaatacg atatataacg attgcgacat
    12181 taccatatcg ccgacccagg cagggccaat gactaaaatc ttcatgcttt gtttttccag
    12241 tataggaatt tcaaaattga ctcataagtt ctaaaaagtt cagcgtatat tttattcgca
    12301 agagtcattt tggatttatc ttttctttta ccaccaatcg tcgaagcaat ctcagctcta
    12361 gtaaaaagat ctggagacac gctgtaaact tgaacaccat gtctccatgg tttttccatg
    12421 tagtcatcga ctggttcaat aaactcttga gcatgcataa caaactttga ggctgattta
    12481 ggtgtgacga gataagcggt ggtaccacaa gtgccagcag agtagccccc tagcatatga
    12541 ttatcatcaa taggtacaat tggggtgaat gcacacttct gagtggcaga caacttgatg
    12601 tagtcataat tagaagtcag tggaagggta tgttcgagca cttgcttcag cccttctacg
    12661 gggtcgacgt tgtcttctaa gatgacgatc ggttcatcaa gctcaataca gcgaacccac
    12721 aactcgtaat ggcttgcaaa gcaggcgact tcactggttt ttaattgata ccctttgcgg
    12781 cgtagagtaa tggttggacg acttttttct gaatgaagaa aactgtcaaa agagccatct
    12841 acggcatcaa aaaattcaaa cgtaatgtct tgttgtttta acttttcttc aatacgttct
    12901 cggcgatcta gcgatcgagt caagcttacg acaaatactt tcactgcact ttccagtcgc
    12961 tgttatatct tggtttaaac aaattgtaat agttattacc gtcttcttga gagtgtagca
    13021 actgcagtac ccacatatat tgttcgggtt tatcaccgac aaaatattca atggcttcat
    13081 tacaggtacg agcgtcttgc tcttcgcctt tatccagctc aaacgccggt aaaatctcaa
    13141 tatcaaacgt accatcttca gtgttcatcg acgcaaatag aggtagtact tttgagcgtg
    13201 atagtttcgc aattttgcct aaaccaggta gcgttgcttt acgtgtagca aaaaaatcga
    13261 caaacacact atgctctggg ccgtgatctt gatcgggtag ataataccct acatagccat
    13321 ctcgaataga ttttagaaaa ggcttaattc caccagagcg ctcgtaaact cggccgccat
    13381 actgaactct ttgcttgtgc attaaccagt cagccacatc attcttttgt ttttttgcca
    13441 tcgcagagac aggcattccc attgaagcga gcaagatagc aggcacatca atagcccaag
    13501 agtggggaac cattaaaatc acattttcgt tgttatcaat gtgctgtcgt aaaatgtcta
    13561 ggccgcgaat ttggcaacgg ttctgaagcc actttggtga gcgtaaactt agtaacccga
    13621 agcgaaaaaa gaacatggct gctgtgtaga gtgttttttc tatcaaagct tcacgttctt
    13681 ggaacgactt ttccggaaaa cacagctcaa agttaaccct cgctcggtga gctggacctt
    13741 tgtgggattt agacagtttt ttagctatgg ttttcgccaa ccacttttgt aaacccagtg
    13801 gcattaatga taaaggtaaa aataagataa cacctaacca cgtgccccag tacttaggcg
    13861 acagaaagtg gcgcttaaac tcagggttat atgctttagg gtcgaagtca tcacgtttaa
    13921 tggtcatggc gtgtctagtt acctattcgt gtactggcga agtggtttaa atgagaaaaa
    13981 gatagttagg aatgatacag gctattgcac ttgagtaaag gtggcaggtt atggttctgc
    14041 caccttgtgg tgcttaacgg ttttggatag ttaagtattc ggcaacgcct tctgcaaccg
    14101 tcttgaactc aacatcacag cctgcgtcgc gcagtttggt cagatcggct tgtgtgaatt
    14161 cttggtaagc gccttttaga tgctcaggga aaggaatagt ttcaatctcg ccttttccat
    14221 gatgggcaat gaccgctttt gctacttctt caaaagattc tgcacgacca gtaccacagt
    14281 tgaagatgcc agaaacacca ttttcaagga accacaggtt tactttacat acatcaccaa
    14341 cgtaaacgaa atcacgctta aaagactcgc tacctgcaaa cagttttgga ttttcacctg
    14401 cgtttagttg gttgttcaga tggaacgcaa cagatgccat gctgcctttg tgatcttcac
    14461 gtgggccata tacgttgaag taacggaagc cggtgatttg agaaagggtc tcaccatgtt
    14521 cttcagcgtc ttgccatagc cgacgaacat agttatcaaa ctgctgtttc gagtaaccgt
    14581 atacattcag tgcgccttcg tattcgcgtt cttcaacgaa agtatctgta tcgccgtaag
    14641 tggctgcaga agaggcgtat aggaatggaa tttcacgatc taaacagtaa tgaagcagct
    14701 ctttagagta ctcgtaattg tttagcatca tgtatttacc atcccactct gtcgttgcag
    14761 agcaagcgcc ttcgtggaaa attgcctcaa tcggaccaaa atcatcgccc gccataattt
    14821 gggtcagaaa gtcgtcacga tccatgtaat ctgtgatgtc cagatcaact aagttcttaa
    14881 actttttgcc attttttagg ttgtcgacaa ccaagatatc gttaatacca gcttcgttta
    14941 gtgcttaac
//
