LOCUS       JQ863079               16590 bp    DNA     linear   BCT 11-NOV-2012
DEFINITION  Vibrio parahaemolyticus strain 1404 serogroup O6 O serogroup
            genetic determinant region genomic sequence.
ACCESSION   JQ863079
VERSION     JQ863079.1
KEYWORDS    .
SOURCE      Vibrio parahaemolyticus
  ORGANISM  Vibrio parahaemolyticus
            Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
            Vibrionaceae; Vibrio.
REFERENCE   1  (bases 1 to 16590)
  AUTHORS   Chen,M., Guo,D., Wong,H.C., Zhang,X., Liu,F., Chen,H., Chen,M.,
            Liu,B., Wang,L., Wu,F. and Feng,L.
  TITLE     Development of O-serogroup specific PCR assay for detection and
            identification of Vibrio parahaemolyticus
  JOURNAL   Int. J. Food Microbiol. 159 (2), 122-129 (2012)
   PUBMED   23072697
REFERENCE   2  (bases 1 to 16590)
  AUTHORS   Chen,M., Guo,D., Wong,H.C., Zhang,X., Liu,F., Chen,H., Chen,M.,
            Liu,B., Wang,L., Wu,F. and Feng,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2012) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..16590
                     /organism="Vibrio parahaemolyticus"
                     /mol_type="genomic DNA"
                     /strain="1404"
                     /db_xref="taxon:670"
                     /note="serogroup: O6"
     misc_feature    1..16590
                     /note="O serogroup genetic determinant region"
     gene            401..1117
                     /gene="kdkA"
     CDS             401..1117
                     /gene="kdkA"
                     /note="KdkA"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="AFV93001.1"
                     /translation="MIEKLQTNNQCLWFNPDLIDATSQAVFDAEYWRKEGKIVGSATG
                     RGTTWFIQLQQLQAALRHYRRGGLFGKLVKDHYWFTGWEKTRAIEEFKLLLRLSESGV
                     NVPRPIAAQATKVGLFYKADLISERIPNASDLVSILQEKSLSEMMYRKIGQEIAKMHN
                     ENVNHTDLNIHNVLIDNNENVWIIDFDKCQVERSTGWQSANISRLKRSFLKEQDKRQI
                     KWQEIDFEALLDGYNQNTTQ"
     gene            1268..1954
                     /gene="wvcE"
     CDS             1268..1954
                     /gene="wvcE"
                     /note="WvcE"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylyltransferase"
                     /protein_id="AFV93002.1"
                     /translation="MINVDVVAVIVARGGSKRIPNKNILPINGISLLERKIDTLKQCP
                     SISRVIVGSEDATILELAKARGAEVVKRPDYYCDEAQASANDMIGNMCSLISADVVVW
                     AHCTNPLVSVDTYESAVNAFFEAEQEGYDSLLSVYELKEHLWDSNFQVLNYNPYAERH
                     TLASELEPLYAQDGAIFIQRHKNMLKNSYFFGAKPKHFIMPVEEVFDINFPVEFKIAE
                     ILLSQCEGDR"
     gene            2211..3401
                     /gene="wvcF"
     CDS             2211..3401
                     /gene="wvcF"
                     /note="WvcF"
                     /codon_start=1
                     /transl_table=11
                     /product="putative UDP-Glycosyltransferase/glycogen
                     phosphorylase"
                     /protein_id="AFV93003.1"
                     /translation="MKKKLLMVCYGGGHVTIIEPLYQQLKDKYEITILALTLAAPYLA
                     ARGVPYVTFRSFKELFEPEVVTTGESLLANLEHNDEVQYEDSLYYMGCSYYDLVASAG
                     LEEAKSLYESKGRAAFYPEPTLIKIIQSLSPDMLITTNAIRAERASLSAAKKCGVPTL
                     CINDGLWIEGGLSGVLDIANDELADIICVLSNEVKSNLEASRKSRSARIVATGTPVFD
                     SIKLTERRKNTNQLTLLFADCHLPKHFHLYPDIVGKPEFAFRVREKLNALAQEHDWKV
                     IVRPHPNQVIDYSKYNNLHISGKNDSLHEQLAQSDIVLTNMSTVGLEGKAMGLGLVTL
                     EGTVFNRYNSFYKAGLSTPVFHENELYNAIIEELERATHGINLYEGLATENISREISR
                     YLGL"
     gene            3410..4879
                     /gene="wvcG"
     CDS             3410..4879
                     /gene="wvcG"
                     /note="WvcG"
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor MviN"
                     /protein_id="AFV93004.1"
                     /translation="MKKIFLSALIVSLGLFIGRFSGFLREIIIANNFGATENSDFIVL
                     LLTTPDFLVNLLMGGAMSMALVPEFKKLNEEESQLLYKQVTSVMFLFGLTVCLLSYPF
                     RSDLISFLALGMNEEFIETNQDYFFFSLLALPFSLATGASLAYLNAKERFTITSLSTL
                     IVNLTIIAFVCLSAFLDSGFILISIGLVFASLLRWLSQVLAIGFLPFFIKHERNLISR
                     GLLSRYFYALAAGGVIFLLPIIVRTVASIDGGGALSLVNYTIKLVELPLIILSTVFSI
                     IFLPKLSGIYSDGNEGKFLKLASNILCFSLFMSILVTTAMVLYADTIVETIYGWGALS
                     HHQLMNISEYLKTYAISLPFQCVNGIILAVFSSRRDTKRPFIVTTLLGIVVFSFLILV
                     KPPIEDLFYSLITFYASTAIYFLIALRAGHKIFPFYVDMRILVLGISLLLMTFVILYT
                     AKSFVITLKPIIGTFTIGVCVTSVLLVFVLMFRFMTRRS"
     gene            4881..5696
                     /gene="wvcH"
     CDS             4881..5696
                     /gene="wvcH"
                     /note="WvcH"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase"
                     /protein_id="AFV93005.1"
                     /translation="MNLMYITNNPEIASYANSCGISRIFVDLEILGKYERQGHLDTVI
                     SNHSEEDINKVKTASGSSEVLVRINPFNNNTSKEVDRAIAEGADIIMLPMFHSKEEVN
                     SVGKMINGRAKFIPLIETKSAAESLETYVDSPYVSEFYIGLNDLHRELGFRFMFEIIS
                     TGYLDQLVAAIKTSGKPFGFGGVARVGEGTLPASLILGEHVRLGSSAVILSRAFHQRS
                     KNLEELKSKLDLELEVSRLYSEIERLKNRTYEQIQHDKNELKRIVDGIVEGEM"
     gene            5846..6247
                     /gene="wvcI"
     CDS             5846..6247
                     /gene="wvcI"
                     /note="WvcI"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFV93006.1"
                     /translation="MYKFRSMKCNASEVGPYFTQQNDPRITRVGRVIRRTSIDELPQL
                     INVLLGDMSIVGPRPDVPVQEKNYTPDEWQLRNSVKPGITGLAQATVRSNTTPEERTR
                     LDLEYVKTHNIYLDLKIIAMTVKQVISKGGN"
     gene            6249..8018
                     /gene="wvcJ"
     CDS             6249..8018
                     /gene="wvcJ"
                     /note="WvcJ"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase"
                     /protein_id="AFV93007.1"
                     /translation="MCGIFGYFDFSNCSISEEMVRAMGEAIVYRGPDGMGYYHREGRA
                     LGNQRLAILDLEHGQQPFYSDEHDIVVVQNGEIFNHVELAQELAGTPFECKTTCDTEV
                     LLRLYQKYGIDFISMLNGMFAISIYDKRIDTMFLIRDRVGEKPLYYANHNGMLAFGSE
                     IKSILKVGIPRELNKKALDSYISYNYVVPPMTMFSGVEHVMPGSYLSISNSGIEEVSW
                     WDVADVNPEEASEATWIEKLDATLDDAVRLRLRADVPFGAFLSGGVDSSSVVGYMAKH
                     LEQPVKTFSIGFHDKKYDESPYAEAAANRFNTEHVCEKVEANLLNLWGTSTYHCDQPH
                     GDVSFMPTYKVAELASKEVKMVLTGDGADELFAGYDKYKNFFDSDTDSLSDEEFQVKF
                     LESISLFQQTSKSKLYSDSMMEALAGVDSHYFAKQVFKKVKHQDRVNQALYIDTKLLL
                     PGNNLVKPDRMGMAVSIENRAPFLDFRMIELAFSIPGELKLHDGETKYIFKKTVSNLI
                     GEELAYRKKQMFTVPIGEWLKTDLKDMVSDLLFSEKAKSRGIFNHDFVQELYENHCKG
                     TSNNTREIRALMAVELWFREFID"
     gene            8083..10002
                     /gene="wvcK"
     CDS             8083..10002
                     /gene="wvcK"
                     /note="WvcK"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-D-quinovosamine 4-dehydrogenase"
                     /protein_id="AFV93008.1"
                     /translation="MSKLKLVVMKEFFLSRRVKDTLVAFSDFNFILLSFWMAVFTRFY
                     QLEYLIDYSSYLYLSISSATLFVFICWVRGVYKHVLRHAMNSIAKLIVSSLCIVVLFL
                     GFTSYFNQVMLPRTLPILFFCFSFILTFFSRLVIVSLIEGASSKGKENIIVYGAGKAG
                     RQLVVMLQKGELYNAKAFIDDNPTLVGRTIVGVKVCSPKNIADLKRKYGIQKAILSIP
                     SAPKKQKAEAASLLSTMGIDVLTIPNLDDLVLGIVKIDELKEVAIEDLLGRIPVAPTQ
                     KLLEANIKGKVVMVTGAGGSIGSELCRQIIKNKPRKLVLYELSEFALYQIDKELNSYI
                     ANEGLDSEVIPLLGSVQRQNRLSAVMSAFKVNTVYHAAAYKHVPLVEHNVVEGVRNNV
                     FGTYYTAMAAIESGVESFVLISTDKAVRPTNVMGATKRLAELGLQALAEQQLHEAKGT
                     RFCMVRFGNVLGSSGSVVPLFKQQIEHGGPITLTHPEITRYFMTIPEAAQLVIQAGAM
                     GKGGDVFVLDMGESVKIIDLAKNLINLSGLEVRDDENPDGDIEIRCTGLRPGEKLYEE
                     LLIGDNVKETGHERIMTAHEIYLPLVQYQEILSKLDEACHNFEHDKIRSILLEAPTGF
                     KPTDGICDLVWKANL"
     gene            complement(10102..11307)
                     /gene="wvcL"
     CDS             complement(10102..11307)
                     /gene="wvcL"
                     /note="WvcL"
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-antigen ligase"
                     /protein_id="AFV93009.1"
                     /translation="MKFIDKVSISKISPHEWLICLPIIWAFSGQLVNSDSYKAMIVLS
                     VISLITSVWNYGCKTIIHNITKNKWTQVVGIMLLSTIFYKEYNSSISNESLRAYAIMF
                     VFLVSFPDKLSDKLFHNLHWLLLISSLFMLTYTTLNMYIWNNPRALWDINPIPYTTIS
                     AAVATGSICYFLTTKTKEIRNISLIAFIFSFNSLVIGLSRGPFLALLCSLFIVVIFTQ
                     RQKCLKYGRLLSILTVAILSFTIHSSLVSERVKSTENAIETIKQGGTDTSIGARIKLW
                     HSSIYSIFEKPILGLGSKEKSFREELANKGIIEQDTAQWGHYHNQYVNILVKQGLVGL
                     ALLIVLFFFPLSQVRKLYTLPASIVLGVLTVFWVSALTDIPVNHAQPLIMFITLISLC
                     WIQSKEEES"
     gene            complement(11428..12705)
                     /gene="kdtA"
     CDS             complement(11428..12705)
                     /gene="kdtA"
                     /note="KdtA"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="AFV93010.1"
                     /translation="MLVRIVYTLLLALASPLLLFGLYKSKPNKPKFGSRWREHFGITP
                     KLKSNDKPIWIHAVSVGESIAATPLIKALKEQNLEQSILVTTTTSTGAEQIAKLGDLV
                     EHRYMPIDFGFAIKGFLKAVQPKQMLIIETELWPNTLHNVHKAGIPITVVNARLSEKS
                     CKNYAKIHRLFNQLHPCLKQVLCQTDSDAERFERLGVEKKKLSVTGSMKFDIQISEQV
                     KQQGQRLRAQLGNDRPVWIAASTHKGEDERVLDAHRQALKSHLNALLILVPRHPERFD
                     DVFTLCQQQGFNTVRRTSTHSVETNTQVYLGDTMGEMLTLMGAADICFMGGSLIGDKV
                     GGHNVLEPAALGVPTITGPSFFNFEDIVVTMKKEGVIDIAQNSQEIALYTVLYMSNKN
                     QRDEISSKLKAFTSKRCGALKSIIQKLNAINGQ"
     gene            complement(12699..13754)
                     /gene="waaF"
     CDS             complement(12699..13754)
                     /gene="waaF"
                     /note="WaaF"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose-LPS heptosyltransferase II"
                     /protein_id="AFV93011.1"
                     /translation="MKILIIGPSWVGDMVMSQSLYKVIKQQHPNCTIDVIAPGWCKPI
                     LERMPEIHQAIEMPIGHGEFNLLGRREIGKSLREKQYDHAYILPKSAKSALIPWFANI
                     PLRTGWKGEMRYGLLNDLRPNMKSFQYMVERYVALAYPKAEMVDSNSLGGLETLPRPS
                     LFIDKQEQGATISKFNLNQESPVVGLCPGAEFGPAKKWPETHYAEVATQMARSGHQIW
                     LFGSQKDLETCNNIRALVPTQFHGHIHVLAGQTSLIEAVDLLAACKTVVANDSGLMHV
                     AAAVGCNVVAVYGSTSPKYTPPLAEKVEIVHTDIDCRPCFQRECQYKHLKCLTELSPK
                     QVLDSIQKLDAITISSC"
     gene            complement(13758..14477)
                     /gene="wvaH"
     CDS             complement(13758..14477)
                     /gene="wvaH"
                     /note="WvaH"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFV93012.1"
                     /translation="MKVIVISLKRSVERRERIASQLEASGIEFSFLDAADAQQGNFPL
                     SERRNDQLTKKRFGYKLVDSEVACFSSHLQAWQIAAESSAPLLILEDNCDLTDEFFEY
                     FKHLPKIAVEFDFLKLCATSPKKYKNIKAFDSKIKIIRYYRRTCGIMGYIISPKAAQS
                     FIHNAAQFIEPVDDYMEKPYKHGIFTYCLKPDLVIRAKIDSTIGSSRKNKQGITLIDK
                     LYIESFRLYEQIKDFSMRAPD"
     gene            complement(14477..15460)
                     /gene="msbB"
     CDS             complement(14477..15460)
                     /gene="msbB"
                     /note="MsbB"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
                     acyltransferase"
                     /protein_id="AFV93013.1"
                     /translation="MTAKRDDYDSKAYNPEFERHFLSPKYWGTWLGVILFLPLALMPL
                     SAQKWLAKTIAKKLSKSHKGPAHRARVNFELCFPGKTFQEREALIEKTLYTAAMFFFR
                     FGLLSLRSPQWLQSRCQIRGLDVLRQHIDNNENVILMVPHSWAIDVPAILLASMGMPV
                     SAMAKKQKNDVADWLMHKQRVQYGGRVYERSGGIKPFLKSIRDGYVGYYLPDQDHGPE
                     HSVFVDFFATRKATLPGLGKIAKLSRSKVLPLFASMNTKDGTFDIEILPAFELDKGEE
                     QDARTCNEAIEYFVGDKPEQYMWVLQLLHSQEDGSNYYNLFKPRYNSDWKV"
BASE COUNT     4842 a   3080 c   3590 g   5078 t
ORIGIN
        1 gaaaacgtaa tgacgacggg taaaccaggt aatactggca tcaaacgcat tgtgaaagcg
       61 acaggttatt ctattcaagg gcttaaagcc gcttttaaac atgaagcagc cattcgccaa
      121 gagttagggt tgctagtgtt cgctatcgtc ttggttagtc tatttgatgt aactatggtt
      181 gaacgtatcc taatgttagg cgttgtcgtc cttgttttaa ttgtcgagtt gctaaactct
      241 gcggtagagg ccgtcgtcga tcgagttggt gtcgagtatc acgagctcag tgggcgagca
      301 aaagatattg gctctgccgc ggttctggtt gctcttggtt ttgccggttt tacctgggct
      361 tacatcctcg caagccatta tttgttttaa cgggtagctt atgattgaga agttacagac
      421 gaataatcaa tgcttgtggt tcaaccctga cctcattgat gcgacgtctc aagctgtttt
      481 tgacgctgaa tattggcgta aagaaggcaa gattgttggg agtgcgacgg ggaggggcac
      541 aacatggttt attcaattgc aacagctgca agcagcgttg cgtcattatc gacgtggcgg
      601 cttgttcggc aagcttgtca aagatcacta ttggtttact gggtgggaga agacccgagc
      661 gattgaagag ttcaagctgt tattgcgctt aagtgagtct ggcgttaatg tcccgcgtcc
      721 aattgcagct caagcaacaa aagtaggtct attttataaa gcggatctaa tcagtgaacg
      781 tattcctaat gcttcagact tggtcagtat cttgcaagag aaatcacttt cagaaatgat
      841 gtatcgtaaa attgggcagg aaattgccaa gatgcataac gagaatgtga atcatactga
      901 ccttaatatc cataacgtac tgatagataa caatgaaaat gtatggatta ttgattttga
      961 caagtgtcag gttgagcgca gcactggatg gcaaagcgcc aatatatcgc gattgaagcg
     1021 ttcattttta aaagagcaag ataaacgcca gataaagtgg caagaaattg actttgaagc
     1081 cttgctcgat ggttataacc aaaacactac gcagtaattt gaatcgacca cccaacccta
     1141 tcaacaaggc tctgattgtg tggtttactc gctatagtca gtgtagactt ggccagttta
     1201 agaaactcta ggattgtgcc atgcttgaca aggtacaatt cttaacgcca atccaatatt
     1261 tatacaggtg attaatgtgg atgttgtagc agtgatagtt gctcgcggtg gaagcaaaag
     1321 aattccgaat aaaaatattc ttccaatcaa tggcattagc ctcctagagc ggaaaattga
     1381 tactctaaaa caatgtcctt ctatcagtcg tgtgatcgtt gggtctgaag atgcaacgat
     1441 tttagaactt gcaaaagcaa gaggcgcaga ggtagttaag aggccagact actattgcga
     1501 tgaagctcaa gctagcgcca acgatatgat aggaaacatg tgtagtttga tttccgcaga
     1561 tgttgttgta tgggcgcact gcacaaaccc attggtatct gtcgatactt atgagagcgc
     1621 tgttaacgct ttttttgagg ctgagcaaga aggttacgac tctttgttgt cggtatatga
     1681 attgaaagag cacttgtggg actctaactt tcaagttttg aactataacc catatgcgga
     1741 aaggcatacg ctggcatcag aactggaacc actttatgca caagacggtg ctatttttat
     1801 tcaaaggcac aaaaacatgt tgaaaaacag ttactttttc ggtgctaagc ctaaacactt
     1861 tataatgcca gtcgaagaag tttttgatat caattttcct gtagagttca aaatcgctga
     1921 gatactacta tctcagtgtg aaggtgatag ataaaaaagc tatttaatac caaaaagaga
     1981 aagccagcag ataataaggc tatcatcata acggctgtcg attgttttat ataataatgc
     2041 tatttgttct ttcgttttat ctaataatac attgtttgca tattggtatc cttaactact
     2101 tatagttttt atatttattt taaataatga ctaggttatt gcgacataga ttaaaagtgc
     2161 agtcaaagag tatcagcaat actgtaaaat cttagtttta aggccaggaa atgaaaaaaa
     2221 aactactaat ggtgtgttac ggtggtgggc acgtaacgat cattgagccc ctttaccagc
     2281 agctcaaaga taaatacgag ataacgatcc tagctttgac tttggccgct ccttacttag
     2341 ctgctcgggg cgtaccgtat gtgacattcc gatctttcaa agagctcttc gagccggaag
     2401 tcgttactac tggtgaaagc ctccttgcaa acctcgagca taatgatgaa gtacaatatg
     2461 aagatagcct atattacatg gggtgctcat actatgatct tgtcgcgagc gcaggtctgg
     2521 aggaggcaaa gtctttgtat gaaagcaaag gaagagctgc tttctatcca gaacccacat
     2581 taataaaaat cattcaatct ctttcacctg atatgcttat aacaacgaat gcaatcagag
     2641 cggaacgagc ttctttgtct gcggcgaaga agtgtggtgt accgacgctt tgtatcaacg
     2701 atgggttatg gattgaaggt gggctttcag gagttttgga tattgctaat gacgagttag
     2761 ccgatattat ttgcgtcctt tctaatgaag ttaaatctaa cttggaagca tcaagaaaaa
     2821 gtcgctcagc tcgaattgtt gccaccggta cgccagtttt tgacagtatc aaattgacag
     2881 aaagaagaaa aaacacgaat cagttaacgc ttttgttcgc tgactgccac ctgcctaaac
     2941 actttcattt atatcctgat attgtaggta aaccggagtt tgcatttaga gtccgagaaa
     3001 agctcaatgc tcttgctcaa gaacatgatt ggaaagttat tgtaaggcct cacccaaatc
     3061 aagttattga ttattcaaaa tacaataact tgcatatctc aggtaagaat gactcacttc
     3121 atgagcagct agctcagtca gatattgttc tgactaatat gagtaccgtt ggcttggagg
     3181 gcaaagccat ggggttggga ttagttacgt tagaaggaac cgttttcaat cgctacaatt
     3241 cattctataa ggctggctta tccacacccg tatttcatga aaatgagttg tacaatgcca
     3301 tcattgaaga gcttgaaaga gccactcacg gcataaacct gtatgaaggc ttagctacag
     3361 aaaatatttc acgtgaaatt tctagatatc taggtttgta gctatactca tgaagaaaat
     3421 cttccttagt gcattaattg tttcgcttgg actgtttatc ggtcgcttta gtggttttct
     3481 tcgtgaaata ataatcgcaa ataactttgg ggcaactgaa aacagtgatt ttattgtttt
     3541 gttgcttaca accccagact ttttagttaa cttactcatg ggaggtgcca tgagtatggc
     3601 actagtccca gagtttaaaa aattaaatga agaagagagt cagttacttt acaagcaagt
     3661 aacgtctgta atgtttcttt ttggcttaac agtttgctta ctttcttatc catttaggag
     3721 cgacttaata tcatttcttg cattgggaat gaatgaagaa tttatagaaa ccaatcaaga
     3781 ttatttcttc tttagcttac ttgcattacc tttttcattg gcaactggtg cttcgttagc
     3841 ttatctaaat gccaaagaaa gattcacaat tacttctttg agtacgttaa tagttaatct
     3901 tactattatt gcatttgttt gcttatcagc ctttttagat agtgggttta tcttaatatc
     3961 gattggcttg gtgttcgcgt ctttattgag gtggctgtca caagtacttg ctataggatt
     4021 tctcccattt tttatcaaac atgaaagaaa cttaataagc aggggtttat tatctagata
     4081 tttttatgct ttggctgcag gtggtgttat cttcctttta ccaataattg tccgtaccgt
     4141 cgcttccatt gatgggggag gggcgttgtc tttggtgaat tacacaatta agttggtaga
     4201 gctgccttta attatcctat cgacagtatt tagtataata tttcttccta aattatcggg
     4261 tatatactcg gatggtaatg agggtaagtt cttaaaacta gcatccaata tattatgttt
     4321 tagccttttt atgtccatac ttgtgacgac agcaatggtt ctttatgcgg atactatagt
     4381 tgaaactatt tatggttggg gggcgttaag tcaccatcag ttgatgaata tttctgaata
     4441 tttgaaaacg tatgccataa gtttgccgtt tcagtgcgtg aacggcatta tactggctgt
     4501 tttttcatca agaagggata ctaagcgacc tttcatagtt actactctat tgggtattgt
     4561 cgtcttttct tttttaatat tggtaaaacc accaattgaa gatttgtttt attctctaat
     4621 aactttttac gcctctacag caatatattt tttgattgct cttcgagccg ggcataagat
     4681 tttcccattc tatgtagata tgagaatact tgtgcttggt atttccttgc tattgatgac
     4741 gtttgttatt ttgtacactg caaagagctt tgttattaca ttaaaaccta ttattggaac
     4801 ttttactata ggagtatgcg taacatcagt tttattagtg tttgttttaa tgtttaggtt
     4861 tatgactaga aggagttagt atgaatttaa tgtatataac taataatcca gagattgctt
     4921 cttatgctaa cagttgtggt attagtcgta tttttgttga cctggaaatc cttggtaaat
     4981 atgagcgtca agggcattta gatactgtaa taagtaatca ttctgaagaa gatatcaata
     5041 aggttaaaac ggcatcaggt tcatctgaag tattagttcg catcaacccc ttcaataaca
     5101 acactagcaa agaagtcgat cgagctattg ccgagggcgc tgatatcatt atgttaccaa
     5161 tgtttcactc aaaagaagag gtgaacagtg ttggtaagat gataaatggc agagccaaat
     5221 tcattcctct tattgagaca aaatctgcag ccgagtctct agaaacttat gttgatagtc
     5281 cgtatgtatc agagttctat atagggttga atgacttgca tagagagtta ggctttcgtt
     5341 ttatgttcga gatcatttca acgggatact tggatcaatt agttgcggca ataaaaactt
     5401 ctggaaagcc atttggcttt gggggcgtgg ctagagtcgg cgaagggaca ttacctgcga
     5461 gtttgatttt gggggaacat gttcgtcttg gttcaagtgc agttatattg tcgagagcat
     5521 tccatcagcg ttctaaaaac cttgaagaac taaagagtaa actcgatcta gagctagaag
     5581 tatcgcgctt gtattccgag atagagagat tgaaaaatag gacttatgag cagattcaac
     5641 atgataaaaa cgaactaaaa agaattgtgg acggcattgt agaaggtgag atgtgaaaac
     5701 tatttttgat tgtttagttt cggcgttggc attgatttta ctcgcgccac ttttgtttgt
     5761 tgttgcgatc atcatcttcg tcgatgatcc aggttcacca ttttttaaac aaaaaagagt
     5821 ggggttagga gggagagagt ttgggatgta taaatttcgt agtatgaaat gcaatgcatc
     5881 cgaagtaggt ccttatttca ctcaacaaaa tgatccaaga atcactagag ttggacgagt
     5941 aatcagaaga acaagcattg atgaattacc gcaacttata aacgtgctat tgggtgatat
     6001 gagcatcgtt ggccctcgtc ctgatgttcc cgtccaagaa aaaaactata cgccagatga
     6061 gtggcaatta cgtaatagcg tcaaaccggg aatcacgggt ttggctcaag ctacggtgcg
     6121 ttcaaatacg acaccagaag agagaacccg attagatcta gaatatgtaa agactcataa
     6181 catttatctc gatcttaaaa ttatagcgat gacagtgaag caagttatta gcaaaggtgg
     6241 gaattaatat gtgcggtatt ttcggttact tcgacttttc taattgttct ataagcgaag
     6301 aaatggtgcg tgccatgggg gaagcgattg tatatcgagg gcctgatggc atggggtatt
     6361 accaccgaga aggtagagca ctcggaaacc agagattagc aatcttagat ttagagcacg
     6421 gacaacagcc tttttattct gatgagcacg atattgttgt ggttcaaaat ggtgaaatat
     6481 ttaaccatgt tgaacttgct caagagcttg ctgggacccc tttcgagtgt aaaacgacat
     6541 gtgatacaga agtattgtta agactttacc aaaagtacgg tattgacttc atctcaatgt
     6601 taaacggtat gtttgcaatc agtatttacg ataagcgcat agacactatg tttctcattc
     6661 gagatcgcgt tggagaaaag ccactatatt acgcaaatca caacggaatg ttggcctttg
     6721 gttcggaaat aaaatcaatc ttgaaggtag gtattccaag agaactaaac aagaaagcat
     6781 tagatagtta tatctcttac aattatgtgg taccacccat gacaatgttt tctggtgtgg
     6841 aacatgtaat gccgggaagc tacctctcta tttctaattc aggcattgaa gaagtttctt
     6901 ggtgggatgt cgcagatgtc aatccagaag aagcaagtga agcgacctgg attgagaaat
     6961 tggacgcgac tttagatgat gcagttcgac tccgcttaag agctgatgtc ccgtttggtg
     7021 cgttcttatc tggaggagta gactcaagct ccgttgttgg ttatatggcg aagcatttag
     7081 agcaaccagt caaaacattt tcgattggct ttcatgataa aaagtacgat gaatccccct
     7141 acgcagaggc tgcggccaac aggttcaata ctgagcatgt ttgcgaaaaa gtagaagcaa
     7201 accttctgaa cttgtggggg acgtcaacat atcattgcga tcaaccgcat ggtgatgttt
     7261 catttatgcc gacgtataaa gtcgcagaac ttgcttcaaa agaagtgaaa atggttctaa
     7321 caggcgatgg ggctgatgaa ctgtttgccg gatatgataa atacaaaaac ttttttgatt
     7381 cagacactga ctcgctaagc gacgaagagt ttcaggttaa gtttttggaa agtatttcgc
     7441 tattccaaca aacatcgaag agtaagctat acagcgattc tatgatggaa gctttggctg
     7501 gggtagacag tcactacttt gcgaaacaag tatttaagaa agtaaagcat caggacagag
     7561 taaaccaagc cttatatata gacaccaagc tattgctgcc tggaaataac ttggttaaac
     7621 cggaccgaat gggaatggcg gtctctattg aaaaccgtgc tccatttcta gactttagaa
     7681 tgattgagct agctttttca attccaggtg aattaaaact acacgatggt gagacgaagt
     7741 acattttcaa aaagactgta tctaacctga taggcgagga gttagcttac cgtaaaaagc
     7801 aaatgttcac agttccaatt ggcgaatggc tcaaaacgga cttaaaggac atggtctccg
     7861 atctcttatt tagcgaaaaa gccaaatcaa gaggaatttt caaccatgat tttgttcaag
     7921 agctttatga aaatcattgt aaaggtacct cgaataatac tcgagagatt cgagctttaa
     7981 tggcagttga actttggttt agagagttta ttgattagtg tattcagtca tgggtttatg
     8041 ctcatggctg attaacaata tgtattctca tgtacgaaat gcatgagtaa gctcaagttg
     8101 gtcgtaatga aagagttttt tttgagccgt agagtgaagg atacactagt agccttctcc
     8161 gattttaact ttatattact gtccttttgg atggcagtct tcacaaggtt ttatcagcta
     8221 gagtacttga tagattactc tagctactta tacctatcta tttcatccgc tactctcttt
     8281 gtctttatct gttgggttcg aggtgtttac aaacacgtgc taagacatgc aatgaatagt
     8341 attgcaaagc taattgtaag ctcattatgt attgtcgtgc tttttttagg ttttacctct
     8401 tattttaatc aggtaatgct gccgcgaaca ttaccaatat tgttcttttg tttttctttc
     8461 attttaacct tcttttctag gttggttatt gtaagtctta ttgaaggggc ttcatcgaaa
     8521 ggtaaagaaa acatcattgt ttatggtgca ggaaaagccg gacgccaatt ggttgtaatg
     8581 ttgcaaaaag gtgagttgta caatgctaaa gcctttattg atgataaccc aactctagtt
     8641 ggaagaacga ttgttggcgt taaggtttgc tcacccaaga atattgcaga cttaaagcgt
     8701 aagtacggta ttcaaaaagc gatcttatcc attccttcag cgccaaaaaa acagaaagcg
     8761 gaagctgctt ctctgctgtc tacaatgggg attgatgttt tgacaattcc aaacttggat
     8821 gatcttgtct taggcattgt aaagatagac gagttaaaag aggttgcgat agaggatttg
     8881 ctgggtcgta taccagtcgc accaacccag aaactgttag aagcgaatat taaaggtaaa
     8941 gttgtaatgg tgactggggc gggaggctct attggttctg agttatgtcg ccagattatt
     9001 aagaataaac caagaaagct ggttctttat gagctttcag agtttgcgtt gtatcagata
     9061 gataaagagt taaatagtta catagcgaat gaaggcttag attcagaggt gattcctttg
     9121 cttggctccg tccaacgcca aaatcgttta tcggctgtga tgagcgcgtt taaagtaaac
     9181 acggtgtacc atgctgcagc atacaaacat gttccattag ttgagcacaa cgttgtagag
     9241 ggcgttagaa acaacgtttt tggtacgtac tacaccgcca tggcggcaat cgagagtggt
     9301 gtcgagtctt ttgtattgat atcaaccgat aaagctgtga ggccgacaaa tgttatggga
     9361 gccacgaaac gtttagctga gctaggctta caggcactcg ctgagcagca acttcatgaa
     9421 gctaaaggta caagattctg catggttcgc ttcggcaatg ttctcggttc atccggctct
     9481 gtcgtgcctt tgttcaagca gcaaattgag catggaggcc cgatcactct gactcaccca
     9541 gagatcactc gatactttat gaccattcca gaagctgcac agttggttat tcaagctgga
     9601 gcaatgggta aaggagggga cgtattcgta cttgatatgg gagagtctgt gaagatcatc
     9661 gacttagcta aaaacctcat taatctttca gggctggagg taagagatga tgagaatcct
     9721 gatggtgata tcgaaatacg ctgtacaggc ttaagacctg gtgaaaagct ttatgaagag
     9781 ctattgattg gagataacgt caaggaaact ggccatgagc gtattatgac agctcatgag
     9841 atctacttac cgcttgttca gtaccaagag atactcagca agttagatga agcttgtcat
     9901 aactttgagc atgataaaat tcggagcata ttgcttgaag ctccaacagg ttttaagccg
     9961 actgatggaa tatgcgacct tgtttggaaa gctaacctgt agaacctcgt aggttaaaat
    10021 gaaagcggcc catggttatc tcatggaccg ttttcaggta aaaagtttag aacgtagcgt
    10081 tttatacact cttgcttagc gttaactctc ctcttcttta ctttgtatcc aacataaaga
    10141 tatgagtgtg atgaacatta tgagtggctg agcatggttt acaggtatgt ccgtaagagc
    10201 tgaaacccag aaaacagtta acacaccaag cacaatgctt gctggcagtg tatacaattt
    10261 tcgtacttgt gatagaggga agaaaaacaa tacgataagt agtgctagcc ctactaggcc
    10321 ttgttttaca agaatattga cgtattgatt atggtagtgc ccccattgag ctgtatcttg
    10381 ctcaataatg cctttattag ctaactcttc tcgaaatgat ttctctttac tgcctaaacc
    10441 gagaatcggt ttctcaaata tcgaatagat acttgaatgc cataacttta ttcttgctcc
    10501 aatcgatgtg tctgttccac cttgtttgat tgtttcaatc gcattttcgg tactttttac
    10561 cctctcggaa acaagagaag aatgaattgt gaaagataaa atagcgacag taagaataga
    10621 tagcaaccta ccatatttaa ggcatttctg tctttgagtg aaaataacga caataaataa
    10681 agagcagagt aaagctaaaa atggccctct tgaaaggcct attaccaatg aattaaagct
    10741 aaatataaaa gctattagac ttatatttcg aatttctttt gttttagtag ttaaaaagta
    10801 acatatcgag ccagttgcca cagctgcgga aattgttgtg tatggtatag ggtttatatc
    10861 ccataatgct ctaggattgt tccaaatata catgttaaga gtagtgtacg ttaacatgaa
    10921 taaactggaa ataagcaaaa gccagtgaag attgtgaaat agtttgtccg atagtttatc
    10981 tggaaaagaa acaaggaaga caaacatgat tgcataagca cgtaaggatt cgttggagat
    11041 tgaactattg tattctttat aaaatatagt tgatagcaac attataccaa caacttgagt
    11101 ccacttattc ttggtgatgt tatggatgat tgttttgcaa ccataattcc aaactgaagt
    11161 aattagagaa attactgaca gtacgatcat cgctttgtaa gaatcagagt ttactagttg
    11221 acctgaaaaa gcccatatta ttggtaggca tattagccat tcgtgtggag atatcttgct
    11281 tatacttact ttatcaataa atttcatttt gacagctctt tgagaactca atatgatggc
    11341 ttatttgtta tcaaggcacc actttattac taagctttat gtgtatgctc agtgcttggc
    11401 ttaaatgtta attttatatt ataaaattta ctgaccattt atagcgttaa gcttctggat
    11461 tatggatttt aaagccccac atcttttaga cgtgaatgct tttagcttgc tacttatttc
    11521 atcccgttga tttttgttac tcatatagag tactgtataa agtgcaattt cctgagaatt
    11581 ttgggcaata tctataacac cttctttctt catggtgacg acaatatctt caaagttgaa
    11641 aaaactaggt ccggttatgg ttggaacgcc caaagccgct ggctctaaca cattgtgtcc
    11701 accgacttta tctccgatta gactgccgcc cataaagcag atgtccgcag cgcccatcaa
    11761 agttagcatc tcgcccatag tatcgccgag atatacttga gtattagtct ctactgaatg
    11821 cgttgaagta cgacgcacag tgttaaagcc ttgctgttgg caaagcgtga agacatcatc
    11881 aaatcgttct ggatggcgag gaactaaaat aagtaaagca tttagatgag actttaaagc
    11941 ttgtctatgt gcatctaaga cccgttcgtc ctcgcctttg tgggtgctag ctgcaatcca
    12001 aactggacga tcgtttccaa gctgagcacg aagccgctgt ccttgctgtt tcacttgctc
    12061 tgatatttga atatcgaact tcatcgaacc ggtaacggat agtttctttt tctccacgcc
    12121 taagcgttca aagcgctctg catctgaatc cgtttggcag agtacctgct tgaggcatgg
    12181 atgaagctgg ttaaaaagcc tatgtatttt ggcgtagttc ttacacgatt tttcagatag
    12241 gcgcgcattc acgacagtta tagggatacc cgctttgtga acattgtgaa gcgtgtttgg
    12301 ccacagctca gtctcaatga tcaacatctg ttttggttga acagctttaa gaaaaccttt
    12361 gatagcgaac ccaaagtcaa ttggcatata gcggtgttca actaaatcac ccaacttagc
    12421 aatctgttca gctcccgtac tcgttgttgt agtaactaga atagattgtt cgaggttttg
    12481 ttccttaagg gctttgatta aaggtgttgc tgctatgctt tcaccaacag atacggcatg
    12541 tatccaaata ggtttgtcgt tgctttttag cttgggagtt atgccaaagt gctctctcca
    12601 gcgagagccg aacttaggct tatttggctt gctcttgtac aaaccaaata aaagtagcgg
    12661 tgacgctagg gcgagtagta aggtataaac aatacgaact aacatgagct tatcgtaatg
    12721 gcgtcaagtt tttggatact atctagcact tgcttaggcg aaagttctgt caagcactta
    12781 aggtgtttgt attgacattc acgctgaaaa caagggcgac aatcaatatc ggtatgaaca
    12841 atctccactt tttctgcgag tgggggggtg tacttcggtg aagttgagcc atacacggca
    12901 acaacattac aaccaactgc agctgccaca tgcattaagc ctgagtcatt cgcgacaaca
    12961 gtcttacatg cagcaagtag atcgactgct tcaattaagc tggtttggcc tgccagaaca
    13021 tgaatgtgcc catggaattg cgtcggaaca agtgcgcgaa tattgttaca agtctctaag
    13081 tccttttgag aaccaaacaa ccagatttga tgcccagatc gagccatttg cgtcgcaact
    13141 tcagcgtagt gtgtttcggg ccattttttc gcgggaccga attccgcacc agggcataga
    13201 cccacaacag gagattcttg gttgagatta aacttagaaa tcgtggcacc ttgctcttgt
    13261 ttatcaataa acaggctagg gcgaggtaag gtttcaagcc cgccaagaga attagagtcc
    13321 accatttctg cttttggata agctaacgct acatagcgct ctaccatgta ctgaaaagac
    13381 ttcatgttag ggcgtaagtc attcaataaa ccatagcgca tttctccttt ccagccagta
    13441 cgaagaggaa tgttggcaaa ccatgggatc agtgctgatt tcgctgattt aggtaagata
    13501 taggcgtggt catattgctt ttcacgtaac gatttaccaa tttccctgcg gcctaataag
    13561 ttaaattcac catgaccgat aggcatttca atcgcttggt ggatttctgg catacgctca
    13621 agaatcggtt tacaccatcc tggagcaatg acatcgatag tgcagtttgg gtgttgctgt
    13681 ttaatgactt tgtatagtga ttgtgacatc accatatcgc caacccatga agggccaata
    13741 atgagtattt tcatggttta atccggtgct ctcatagaaa agtcttttat ttgttcataa
    13801 agcctaaaag actcaatata aagcttgtcg ataagagtta taccttgttt gttcttcctt
    13861 gagctgccaa tagttgagtc tatttttgct cgaataacta aatcaggttt gaggcagtat
    13921 gtgaaaatac cgtgtttata tggtttttcc atataatcat cgacaggctc gataaactga
    13981 gcagcattgt gaatgaatga ctgtgcagct tttggtgaaa tgatatagcc cataattcca
    14041 cacgttcttc tgtaataacg gatgattttt atttttgaat caaaagcttt gatgttttta
    14101 tacttctttg ggcttgttgc gcatagcttt aaaaaatcga actcaaccgc gatctttggc
    14161 agatgtttaa agtactcaaa aaattcatca gtaagatcac agttatcttc taatatcaac
    14221 aaaggagcac tggattcagc tgcgatctgc caagcttgaa ggtggctgga aaaacaagca
    14281 acttcgctat caacaagttt ataaccaaat cttttttttg ttagttgatc gtttctacgt
    14341 tcagataaag ggaagtttcc ttgttgagcg tcagcggcat ccaaaaagct aaactctatt
    14401 cctgacgctt ctaattgtga tgcaatacgc tctcttcgtt cgacagagcg ctttaaactg
    14461 ataactatga ctttcattac actttccagt cgctgttata tcttggttta aacaaattgt
    14521 aatagttact gccgtcttct tgagagtgca gcaactgcaa tacccacata tattgttcgg
    14581 gtttatcacc gacaaagtat tcaatggctt cattacaggt acgagcgtct tgctcttcgc
    14641 ctttatccag ctcaaacgct ggtaaaatct caatatcaaa cgtaccatct ttagtgttca
    14701 ttgacgcaaa tagaggcagt acttttgagc gtgatagttt tgcaatttta cctaaaccag
    14761 gtagtgttgc tttacgtgta gcaaagaaat ctacaaacac actgtgctct gggccgtgat
    14821 cttgatcggg tagataatac cctacatagc catctcgaat ggatttcaga aaaggcttaa
    14881 tgccacctga gcgttcataa acacgaccac cgtattgaac tctttgcttg tgcatcagcc
    14941 agtcagcaac atcattcttt tgtttttttg ccatcgcaga gacaggcatc cccattgaag
    15001 ccagcaaaat agcaggaaca tcgatagccc acgagtgagg caccatcaaa atcacatttt
    15061 cattgttatc aatgtgctgg cgtagtacgt ccaatcctcg aatttggcag cgactctgaa
    15121 gccattgagg agaacgcaaa ctcagcaacc caaaacgaaa aaagaacata gctgcggtgt
    15181 agagcgtttt ttcaattaag gcttcgcgtt cctgaaacgt ctttccaggg aagcataatt
    15241 caaagttgac tcgagccctg tgagctggcc ctttatgaga tttagataac tttttagcta
    15301 ttgttttggc tagccacttt tgggcgctaa gaggcattaa ggctagcggc aaaaagagta
    15361 ttacacctaa ccaagttccc caatacttag gcgacaaaaa atgacgctca aattcagggt
    15421 tatatgcttt ggagtcgtag tcgtcacgtt ttgctgtcat ggcgtgtcta gttacctatc
    15481 cgtcttgatt tactcgtatg agagcaaggt gataaaaata agatagtaga gatgatacag
    15541 gctattggaa tggagtaaag gtggcaacaa gtgatgctac cacctaaatg atgcttaact
    15601 gttttggata gttaagtatt cggcaacgcc ttcagcaacc gtcttgaact caacatcgca
    15661 accggcagcg cgtagcttgg taaggtctgc ttgcgtgaac tcttgatagg caccttttag
    15721 atgctctggg aaaggaatag tttcaatctc gccttttcca tgatgggcaa tgaccgcttt
    15781 tgctacttct tcaaaagatt ctgcacgacc agtaccacag ttgaagatgc cagaaacacc
    15841 attttcaagg aaccacaggt ttactttaca tacatcacca acgtaaacga aatcacgctt
    15901 aaaagactcg ctacctgcaa acagttttgg attttcacct gcgtttagtt ggttgttcag
    15961 atggaacgca acagatgcca tgctgccttt gtgatcttca cgtgggccat atacgttgaa
    16021 gtaacggaag ccggtgattt gagaaagggt ttcaccatgt tcttcagcgt cttgccatag
    16081 acgacgaacg tagttatcaa actgttgttt cgagtaaccg tatacattca gtgcgccttc
    16141 gtattcgcgt tcttcaacga aagtatctgt atcgccgtaa gtggctgcgg aagaggcgta
    16201 taggaatgga atttcacgat ctaaacagta atgaagcaac tcttttgagt attcatagtt
    16261 gtttagcatc atgtatttgc cgtcccactc tgtcgttgca gagcaagcgc cttcgtggaa
    16321 aattgcctca atcggaccaa aatcatcgcc cgccataatt tgggtcagaa agtcgtcacg
    16381 atccatgtaa tctgtgatgt ccagatcaac taagttctta aactttttgc cattttttag
    16441 gttgtcgaca accaagatat cgttaatacc agcttcgttt agtgctttaa caatgttgct
    16501 gccgatcatg ccagcgccac cagttacgat gatcatagaa cttccaccat agattaaaat
    16561 tttctcagga attctagcaa gaaacagaaa
//
