LOCUS       JQ863080               15121 bp    DNA     linear   BCT 11-NOV-2012
DEFINITION  Vibrio parahaemolyticus strain 20577 serogroup O7 O serogroup
            genetic determinant region genomic sequence.
ACCESSION   JQ863080
VERSION     JQ863080.1
KEYWORDS    .
SOURCE      Vibrio parahaemolyticus
  ORGANISM  Vibrio parahaemolyticus
            Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
            Vibrionaceae; Vibrio.
REFERENCE   1  (bases 1 to 15121)
  AUTHORS   Chen,M., Guo,D., Wong,H.C., Zhang,X., Liu,F., Chen,H., Chen,M.,
            Liu,B., Wang,L., Wu,F. and Feng,L.
  TITLE     Development of O-serogroup specific PCR assay for detection and
            identification of Vibrio parahaemolyticus
  JOURNAL   Int. J. Food Microbiol. 159 (2), 122-129 (2012)
   PUBMED   23072697
REFERENCE   2  (bases 1 to 15121)
  AUTHORS   Chen,M., Guo,D., Wong,H.C., Zhang,X., Liu,F., Chen,H., Chen,M.,
            Liu,B., Wang,L., Wu,F. and Feng,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2012) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..15121
                     /organism="Vibrio parahaemolyticus"
                     /mol_type="genomic DNA"
                     /strain="20577"
                     /db_xref="taxon:670"
                     /note="serogroup: O7"
     misc_feature    1..15121
                     /note="O serogroup genetic determinant region"
     gene            397..1107
                     /gene="kdkA"
     CDS             397..1107
                     /gene="kdkA"
                     /note="KdkA"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="AFV93014.1"
                     /translation="MIQQYRDSNQVIWFDEELIEDPSQPIFDAEYWQATNKVTGSASG
                     RGTTWFVQLDTMQAALRHYRRGGLFGKLVKDNYWFSGWEQTRCAQEFQLLLTLINAGV
                     HVPRPIAARAVKSGLTYQADLLSERIPNARDLVSILQEKPLPEGMYQKIGQEIAKMHN
                     AGVNHTDLNIHNILIDDKDKVWIIDFDKCRKQEHGDWKKQNLERLLRSFKKGLLKRQI
                     IWQASDFDHVQKSYTENT"
     gene            1200..2264
                     /gene="rmlB"
     CDS             1200..2264
                     /gene="rmlB"
                     /note="RmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-D-glucose 4,6-dehydratase"
                     /protein_id="AFV93015.1"
                     /translation="MKILVTGGAGFIGSAVVRHIIRDTQDSVVNLDKLTYAGNLESLV
                     DVADSDRYYFEQVDICDRTELDRVFSEHQPDMVMHLAAESHVDRSIDGPAAFIETNVM
                     GTYHLLEAARQYWSSLEEANKSAFRFHHISTDEVYGDLEGTDDLFTETTSYAPSSPYS
                     ASKASSDHLVRAWQRTYGLPTLVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGD
                     GMQIRDWLFVEDHARALYKVVTEGEIGETYNIGGHNEKANIEVVKTICALLEELRPDK
                     PAGVESYESLITYVKDRPGHDVRYAIDATKIAQELNWTPEETFESGIRKTVEWYLNNP
                     QWWQRVLDGSYSLERLGAGE"
     gene            2264..3130
                     /gene="rmlA"
     CDS             2264..3130
                     /gene="rmlA"
                     /note="RmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AFV93016.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIREILIITTPEDQEGFQRLLGDGSEFGIELQYAIQPSPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIFYGQGFTPKLQQAAALTEGATVFGYKVKDPERFGVVEFDEEMRALSIEEK
                     PFKPKSNYAVTGLYFYDNRVVDIAKTIQPSHRGELEITSINQAYLEQGKLTVDVLGRG
                     FAWLDTGTHGSLLEAAHFVETIEKRQGYKVACLEEIAYRRGWLTRDHIQAAGKALSKN
                     DYGRYLLELLEE"
     gene            3131..3544
                     /gene="wbtA"
     CDS             3131..3544
                     /gene="wbtA"
                     /note="WbtA"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-3,4-keto-hexulose isomerase"
                     /protein_id="AFV93017.1"
                     /translation="MSNIPLISFQKMGDERGSLISLEQHKNIPFDIKRIYYIFDTQSG
                     LARGFHAHYDLEQVAICMKGSVTFVIDDGTTKEKVTLSSPDVGLHIKGLKWREMHDFS
                     EDCVLMVVASELYDEADYIRDYAEFLNVVNKHAEK"
     gene            3531..4052
                     /gene="wvcM"
     CDS             3531..4052
                     /gene="wvcM"
                     /note="WvcM"
                     /codon_start=1
                     /transl_table=11
                     /product="butyryltransferase"
                     /protein_id="AFV93018.1"
                     /translation="MLKSKTIQLRLVQEDDAEFITQLRGDEKYNTHLSKCDTSVEAQR
                     LWIRAYKEDEQQKKQFYFIIERLDGTKCGTVRLYDFKGVSFCWGSWILNHEKTRYAAL
                     ESALLVYQFGFDVLGFKKCHFDVRKDNHAVNKFHKKFGARLVGDTDLDYLYEIYPSDI
                     EPMKLKCEKILGR"
     gene            4052..5161
                     /gene="wvaD"
     CDS             4052..5161
                     /gene="wvaD"
                     /note="WvaD"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase"
                     /protein_id="AFV93019.1"
                     /translation="MNVNFLELKRLNAEYEDELKQACARVIESGWYILGKETEQFEQA
                     FADYCGVKHCIGVANGLDALTLTLKAWRELGKLNKGDEVIVPGNTYIASVLAVTEADL
                     VPVFVEPDEQTHNISPEAIEAAITEKTKVILPVHLYGQLADMPAIMALATKHNLLVLE
                     DSAQSHGATLDGKKSGAWGDASGFSFYPGKNLGALGDGGAITTNDEELAETLRALRNY
                     GSHVKYQNLYQGLNSRLDEIQSAMLSVKLNYLPAQTKARQELAAFYLREINNPNIILP
                     EVTEPEAHVWHLFVVRTSHRDALVKHLSEHGIQTQVHYPIAPHKQQAYKEFGHLSLAL
                     TEQLQNEVLSLPMSSYLSEDESQEVVDAINAFKVE"
     gene            5161..6093
                     /gene="wvcN"
     CDS             5161..6093
                     /gene="wvcN"
                     /note="WvcN"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFV93020.1"
                     /translation="MTQEHVQVLEDVVYVFFCYKQSRYLEKTLPAALNQTVWPSQLLI
                     LDDCSPDDSHKIILSLLEFAPKGLNIEYRHNKQNVGLVSQINSLRGQYQNKLIIVQAG
                     DDIAMPSRVEETYEAWFKNGKPSLIIGGYDKISEQGDVIESFESIKQNKKPYTLENII
                     NRKCAVDGCCAAFTSDVLNEFEPYNIDIINEDRINVLRAFLLNGIHHEHKKWIQYRLG
                     GISTFAKETREEKYNNTVINAQRELQDLKMNLRDAQSKNANEAVSAIKKRMRTAEFMS
                     SLPQSNFALYIEAICYLVKTLDYRTVLKVLRRLK"
     gene            6090..6953
                     /gene="wvcO"
     CDS             6090..6953
                     /gene="wvcO"
                     /note="hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="WvcO"
                     /protein_id="AFV93021.1"
                     /translation="MSTIKNIKQSISMKRHFASTFRSFKKRSDKSTDSPKDFYRTYEK
                     CTVLGNGPSLKSDINNISKDTDVIAVNHFSESEHFTTLKPNKYILIDSYFWDEATHSS
                     LVEKRDKLFKDLNELVSWKMQLIIPDFADIAFIQGNIKNSNIAVKVLRAIPSNVTNIK
                     YGPIALSSGIVAPPAVNVLIFAVYVAILCEYDSVEIFGADMSFHNDVHVDQKNNQLYM
                     VYKHFYGKEEKLPLLENPQKVKPFNMTDLMRLTFLTFYAHQLLNKWADLKGVKIVNRS
                     SFSMIDAYPRR"
     gene            complement(7002..7682)
                     /gene="wvcP"
     CDS             complement(7002..7682)
                     /gene="wvcP"
                     /note="WvcP"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="AFV93022.1"
                     /translation="MSVHVFLPCRKGSERIPNKNTKDFAGVKGGLLKIKLDQLINITD
                     LDSIVVSSNDDVVLEFASTYKDSRIRIDERPEHLGSSSTSTDDLIRYVPSIITTGHVL
                     WTHVTSPFLDESGYSAIIKQYNQALTEGYDSLMTVKELRGFIWNKDKPISYDRNIEKW
                     PRTQTIEPLYEIDSSVFLNSVENYKKLNDRIGLKPSMYIQDAESSIDIDWPEDFTFAE
                     SMWLQKNK"
     gene            complement(7685..8320)
                     /gene="wvcQ"
     CDS             complement(7685..8320)
                     /gene="wvcQ"
                     /note="WvcQ"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="AFV93023.1"
                     /translation="MHPLFNGYDTVIFDCDGVILDSNNLKIDAMRNALRMTQVSELEI
                     DSCCNYFKANFGKSRFHHVSYFVEHFIINADETLCERILNFYSKQCEELYMKAQLTPN
                     FMVAITDCPAKLYVASGSAQDELREVFVKRKLAQYFTLILGSPTAKADNVATILKESS
                     GAALMIGDALSDFHAAKENNIDFLGYLPFSNVKEELESLSKSEGFNILRSW"
     gene            complement(8304..9914)
                     /gene="wvcR"
     CDS             complement(8304..9914)
                     /gene="wvcR"
                     /note="WvcR"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="AFV93024.1"
                     /translation="MQKILDCTLRDGGYYNHWDFSPEVVDAYLTAVAKAKIDYVELGL
                     RNYPKSVYSGPFAYTTEEFLNTLHLPKGPTYGVMVDAKTLLDANKPVEAAINDLFVPA
                     QESKVDLVRIAAHFNEAEQCESMIKAFKEMGYIVGLNLMQAGGKPSEVIAEKVQTVAK
                     ANPDVIYFADSLGNMDSAEVTRIAEIVKQNWDGDIGIHTHNNMGQAMSNTMTARSNGV
                     TWLDVTVTGMGRGAGNAQTENLLAELDGLDKYLPTAIYELVIRHFEPMQRRYGWGSSL
                     LYYLGAKNNIHPTYIQNMLSNPNYGTEEIVGAIEHLKKLEGTTSYNGDVLEEALTVGK
                     ISQPTESQSDLSGIFSGKEILLVTNTPNTATHRVAIETYIKTRKPIVIGINTKHEINT
                     ELFDYFAVSHNSKYLSESSHYSEVAKPVFLPKNRFDEDELSKFSNISMIDYSSTIEKD
                     TLVAHKNYCTLPYDNTAAYALCIAFIGQAQKISLVGFDGYDVTDRRQMEMNEIFSMVS
                     QQFGSTSIQALTPTTYPVKAGSIYAPAI"
     gene            complement(10018..11292)
                     /gene="kdtA"
     CDS             complement(10018..11292)
                     /gene="kdtA"
                     /note="KdtA"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="AFV93025.1"
                     /translation="MLVRIVYTLLLALASPLLLFGLYKSKPNKPKFGSRWREHFGITP
                     KLKSNDKPIWIHAVSVGESIAATPLIKALKEQNPEQSILVTTTTSTGAEQIAKLGNLV
                     EHRYMPIDFGFAIKGFLKAVQPKKMLIIETELWPNTLHNVHKVGIPITVVNARLSEKS
                     CHNYAKIQRLFNQLHPCLTQVLCQTDSDAERFERLGVNKEKLSVTGSIKFDIQISDHV
                     KQQGEALRAQLGKDRPVWIAASTHKGEDELVLDAHRQVLKSHPNALLVLVPRHPERFG
                     DVFTLCQHQGFNTVRRTSTHAVETNTQVYLGDTMGEMLTLMGAADICFMGGSLVGNKV
                     GGHNVLEPAMLEKVIITGPSYFNFKEIVENLLAVDGILIVPDSTTLALRTTEIISQQD
                     KYLLRALHANKWASSNQGALNRTMSLLPKSDK"
     gene            complement(11286..12341)
                     /gene="waaF"
     CDS             complement(11286..12341)
                     /gene="waaF"
                     /note="WaaF"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose-LPS heptosyltransferase II"
                     /protein_id="AFV93026.1"
                     /translation="MKILVIGPAWVGDMVMSQSLYIVLKQRHPDSQIDVIAPGWCKPI
                     LERMPEIHQAIEMPIGHGEFNLLGRREIGKSLREKQYDHAYILPKSAKSALIPWFANI
                     PLRTGWKGEMRYGLLNDLRPNMKSFQYMVERYVALAYPKVEMVDSNSLGGLETLPRPR
                     LAINATEQQATLKKFNLTQDQPIIGLCPGAEFGPAKKWPETHYAEVATQMARSGHQIW
                     LFGSQKDLETCNNIRALVPTQFHENIHVIAGQTSLIEAVDLLAACKTVVANDSGLMHV
                     AAAVGCNVVAVYGSTSPKYTPPLAEKVKIVHTDIDCRPCFQRECQYKHLKCLTELSPK
                     QVLDSIQKLDAITISSC"
     gene            complement(12338..13060)
                     /gene="wvaH"
     CDS             complement(12338..13060)
                     /gene="wvaH"
                     /note="WvaH"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFV93027.1"
                     /translation="MKVFVVSLARSLDRRERIEEKLKQQGITFEFFDAVDGSVDSFLH
                     SEKSRPTITLRRKGYQLKTSEVACFASHYELWVRCIELDEPIVILEDNVDPVEGLKQV
                     LEHTLPLTSNYDYIKLSATQKCAFTPIVPIDDNHMLGGYSAGTCGTTAYIVTPKAASK
                     FVMHAQEFIEPVDDYMEKPWRHGVQVYSVSPDFFTRAEIASTIGGKRKDKSKMTLANK
                     IYAELFRTYESILKFLYWKNKA"
     gene            complement(13057..14043)
                     /gene="msbB"
     CDS             complement(13057..14043)
                     /gene="msbB"
                     /note="MsbB"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
                     acyltransferase"
                     /protein_id="AFV93028.1"
                     /translation="MTAKRDDYDSKAYNPEFERHFLSPKYWGTWLGVILFLPLALMPL
                     SAQKWLAKTIAKKLSKSHKGPAHRARVNFELCFPGKTFQEREALIEKTLYTAAMFFFR
                     FGLLSLRSPQWLQSRCQIRGLDVLRQHIDNNENVILMVPHSWAIDVPAILLASMGMPV
                     SAMAKKQKNDVADWLMHKQRVQYGGRVYERSGGIKPFLKSIRDGYIGYYLPDQDHGPE
                     HSVFVDFFATRKATLPGLGKIAKLSRSKVLPLFASMNTEDGTFDIEILPAFELDKGEE
                     QDARTCNEAIEYFVGDKPEQYMWVLQLLHSQEDGNNYYNLFKPRYNSDWKVQ"
BASE COUNT     4322 a   3036 c   3347 g   4416 t
ORIGIN
        1 aggaaaaaaa cttggggaag ccaggcaata ctggggtaaa gcgcattatc aaagcgacag
       61 gttattctat acagggatta aaagccgctt ttaaacatga agctgctgtt cgacaagagc
      121 tcgctttgct aagcattgct gtgattttgg cgtgcgtggt tgatgtcggg atgatagagc
      181 gcatcttatt gattggtgtc gtggtattag ttcttattgt cgaactgatt aactccgcga
      241 tagaagcggt ggtggatcgc attggtgttg aacgccatga attaagtggt cgcgctaaag
      301 atataggatc ggcagccgtt atggttgcgt tggcgttcgc agcttttact tggctataca
      361 tattggccag tcgctaccta ggctagaggt tacaacatga ttcagcagta tcgagatagc
      421 aaccaagtca tttggtttga tgaggagctt attgaagatc ctagtcagcc tatttttgat
      481 gccgagtatt ggcaagccac caacaaagta actggcagtg cgtcagggcg tggcacgact
      541 tggtttgttc agcttgatac gatgcaagcc gctctgcgtc attatcgacg tggtggcttg
      601 tttggtaagt tagttaaaga caattattgg ttttcaggtt gggagcaaac gcgttgtgcg
      661 caagagttcc aactattact tacactgatt aatgctggcg ttcatgtgcc aaggccaatt
      721 gctgcgagag ctgtgaaatc tggattgaca tatcaggccg atttattgag tgagcgcatt
      781 cctaacgcaa gagacctggt cagtatcttg caggaaaaac cgttaccgga aggaatgtat
      841 caaaaaatag ggcaagaaat tgccaaaatg cacaacgctg gcgttaatca cacagacctg
      901 aatatccaca acatcttgat cgatgacaaa gataaagtgt ggattattga ttttgataag
      961 tgccgcaaac aagagcacgg cgactggaaa aagcaaaact tagaaaggct tcttcgctca
     1021 ttcaaaaaag gattgctcaa acgccaaatc atttggcaag cgtccgattt tgatcatgta
     1081 caaaaaagtt atactgaaaa tacgtaaagt gagctttttg tcagttattc gtgagacaat
     1141 ccaatagcgc atttcggtac acccactaaa agtacccatt aaagtaggta acatttgcaa
     1201 tgaaaatact agtaacaggc ggggcaggct tcattggctc agctgtcgtt cgacacatta
     1261 ttcgtgacac ccaagatagc gtcgtgaatt tagacaagct cacctacgca ggaaacttgg
     1321 agtcgttggt ggatgttgcg gacagtgacc gttattactt cgagcaagtg gatatctgtg
     1381 accgaactga actagaccgc gtgttttcag agcatcaacc cgatatggtg atgcatttag
     1441 cggctgagtc gcatgtcgat cgctctattg atggtcctgc tgcttttatt gagaccaacg
     1501 tcatgggcac ttaccatctt cttgaagcgg cgcgtcaata ttggtcaagc cttgaggaag
     1561 cgaacaaatc ggcgtttcgt tttcatcata tttctacgga tgaagtgtat ggcgatttag
     1621 agggtacgga tgatttattc accgaaacca cctcatacgc tccaagcagc ccatactcgg
     1681 cgtctaaagc gtcgagcgat caccttgtac gcgcttggca gcgtacttat ggtctcccaa
     1741 cactcgtaac caattgctca aacaactatg gtccgtacca tttcccagag aagttgatcc
     1801 cactgatgat tctgaatgcc ctcgatggca agcctttacc tgtgtatggt gatggcatgc
     1861 aaatccgaga ttggctgttt gtggaagacc atgctcgcgc gctttacaaa gtcgtgacgg
     1921 aaggtgaaat cggtgagacc tacaacatcg gtggccataa cgaaaaagca aacatcgaag
     1981 tagtgaaaac catttgtgct ttgcttgaag agctgcgacc agacaagccg gcaggtgttg
     2041 agtcttatga atctttgatc acttatgtca aagatcgccc aggtcatgat gtgcgctacg
     2101 caattgatgc caccaagatt gctcaagagc ttaactggac gccagaagaa acctttgaaa
     2161 gcggcattcg caaaaccgtg gaatggtacc tcaataatcc gcaatggtgg caacgcgtgc
     2221 ttgatggttc atacagttta gagcgattag gagcgggcga ataatgaaag gcattatttt
     2281 agcaggtggc tcgggcactc gcctttaccc tattacacgc ggtgtctcta agcagttact
     2341 gccggtttat gacaaaccaa tgatttacta cccgttgtcg gtactcatgc ttgctggcat
     2401 tcgtgaaatc ttgatcatca ccacgccaga agaccaggaa ggatttcagc gcctattggg
     2461 tgatggctca gagtttggca ttgagttgca atacgcgatt caaccaagtc cagatggctt
     2521 agctcaagcc ttcatcatcg gtgaagaatt cattggcgat gactcagtat gtttggtgtt
     2581 gggtgacaac atcttttatg gacaagggtt cactccgaag ttgcagcaag ccgctgcttt
     2641 aacggaaggt gcgacggtct ttggctataa agtgaaagat cctgagcgct ttggtgtggt
     2701 ggaatttgat gaagagatgc gagcgctatc gattgaagag aagccattca agccaaaatc
     2761 caattacgcc gtgaccggac tgtatttcta cgataaccgc gtcgtggaca ttgccaaaac
     2821 tattcaaccg tctcatcgtg gcgagttaga aattacgagt attaaccaag cttacttaga
     2881 gcaagggaag ttgacggttg acgtgctagg gcgcggcttt gcgtggttag acacggggac
     2941 acatggcagc ttgcttgaag ctgctcactt tgtcgaaacg atcgagaaac gacaaggtta
     3001 taaagtggct tgcttagaag agattgctta tcgccgaggg tggttaacac gagatcatat
     3061 tcaagctgca ggaaaagcct tatctaaaaa cgattatggc cgctatcttc tagagttatt
     3121 ggaagaataa atgtcaaata taccgctaat atcttttcag aaaatgggtg atgagcgagg
     3181 ttcactaatc tcgttagagc aacacaaaaa cattccgttt gatatcaaac gtatttacta
     3241 catctttgat acacaaagcg gtttagctag gggctttcat gcacactacg acttagagca
     3301 agtcgctatc tgtatgaaag gtagcgtgac tttcgtcatt gatgatggca ccacgaaaga
     3361 gaaagtgacg ctttcatctc ccgatgtagg actgcatatc aaaggcttaa aatggcgtga
     3421 gatgcacgac ttttctgaag attgtgtgct aatggttgtc gctagtgaat tgtatgatga
     3481 agccgattac atacgcgatt atgctgaatt tttgaatgtg gtaaacaaac atgctgaaaa
     3541 gtaagacaat acaacttcgc cttgttcaag aagatgatgc agagtttata actcagcttc
     3601 gaggtgatga aaaatacaat acacacttgt ccaaatgcga tacgagtgtt gaagcacaaa
     3661 gattatggat aagagcgtat aaagaggacg agcaacaaaa gaagcagttt tacttcatta
     3721 tagagaggct agatggaacc aaatgcggaa cagttagact ttacgacttt aaaggtgttt
     3781 ctttttgttg gggaagttgg atcctgaatc atgaaaaaac acgttacgct gccctcgaaa
     3841 gtgctctttt agtttatcaa tttggctttg atgtgttggg ttttaaaaaa tgccacttcg
     3901 atgttcgcaa agataaccat gcggtaaaca aattccataa aaagttcggt gctcgactcg
     3961 ttggcgatac agacttggat tatctttatg aaatataccc gtcagatatt gaaccaatga
     4021 aattgaagtg tgaaaaaatc ctaggtagat aatgaacgtt aattttttag agctaaagcg
     4081 cttaaatgcc gagtacgaag acgaattaaa acaagcctgt gcacgcgtga ttgaatctgg
     4141 ctggtatatc cttggcaagg aaacggaaca gtttgaacaa gcatttgctg actactgtgg
     4201 tgttaaacac tgtataggcg ttgcgaatgg cttagatgct ttaacactga cgttaaaggc
     4261 ttggcgagag ctaggcaaac ttaacaaagg tgatgaagtt atcgtaccgg gcaataccta
     4321 tatcgcatcg gtcttggcgg tcaccgaagc ggacttggtt ccggtgtttg tggagccgga
     4381 tgagcagacg cacaatatca gtcctgaagc gattgaagcg gcgattacag aaaagacgaa
     4441 agtcattttg ccggtgcacc tgtacggcca gttggccgat atgccagcga ttatggcgtt
     4501 ggcaacaaaa cacaatcttc tcgtgttgga agacagcgcc cagtctcatg gtgcgacact
     4561 tgatggtaag aagtctggag catggggtga cgcctcaggc tttagcttct atccgggtaa
     4621 aaacctaggt gcattgggtg atggtggcgc tattacgacc aacgatgaag agcttgccga
     4681 aaccttgcgt gcccttcgaa actacggttc acatgttaaa taccagaatc tttatcaagg
     4741 attgaacagt cgtttggatg aaattcagtc ggcgatgttg tcagtgaaac tgaactacct
     4801 tccagcgcag acgaaagcgc gtcaggagtt ggcggcattt tacttacgcg aaatcaacaa
     4861 cccgaatatt atcttgccgg aagtgacaga gcctgaggca catgtatggc atttgtttgt
     4921 ggtgcgaact tcacaccgag atgcattggt taagcattta tcagagcatg gtattcaaac
     4981 acaagtacat tacccgatag cgccgcataa acagcaggct tataaagagt ttggtcatct
     5041 gtcattagcg ttgactgagc agttacaaaa tgaggtgctg tcattgccaa tgtcgtccta
     5101 cttgagtgaa gacgagagtc aagaagtagt cgatgcaatc aacgctttta aggttgagta
     5161 atgacacaag agcatgtgca agtattagaa gacgtggttt atgtcttctt ttgttataaa
     5221 caatctcgtt atcttgagaa aacattgcca gcggcattga accagaccgt gtggccaagc
     5281 caattattga tcttggatga ctgttcacct gatgatagtc ataaaataat tctatcgctt
     5341 ttagagtttg cgcctaaagg attaaatatt gaatatcgcc acaataagca aaatgttggt
     5401 ttagttagcc aaatcaactc cttgcggggg caataccaaa ataagcttat tattgtacaa
     5461 gctggcgatg atatagcgat gccgagccga gttgaggaaa cttatgaagc ctggtttaaa
     5521 aatggcaaac caagcttaat tattggtggt tacgacaaaa tttctgagca aggcgatgtt
     5581 attgagtcat ttgaatcgat taagcaaaat aaaaagccgt acacactcga aaatattatt
     5641 aatagaaagt gtgcagtaga tggctgttgt gctgctttta cctctgatgt attgaatgag
     5701 tttgagccct acaacataga catcatcaat gaagaccgga tcaatgttct ccgagccttt
     5761 ttgttaaatg gtattcatca tgaacacaaa aagtggattc aatatcggct aggtggcata
     5821 tcaacgttcg caaaagaaac acgcgaagaa aaatataaca acacagtgat caatgctcag
     5881 cgtgagctgc aagatcttaa aatgaactta cgagatgcgc agagtaaaaa tgccaatgaa
     5941 gctgtgagtg cgattaaaaa acggatgcgt actgctgagt ttatgtcatc acttccacaa
     6001 tcaaattttg ctttgtacat cgaagcgatt tgttatcttg ttaaaacatt ggattatcga
     6061 acggttttaa aagttttgcg taggttaaaa tgagtacaat taaaaatata aagcaatcga
     6121 taagtatgaa gaggcatttt gcttcaactt tccgctcatt caaaaaacga agtgataaaa
     6181 gtactgactc acctaaagac ttttatcgta catatgagaa atgtactgtt ttaggtaatg
     6241 gaccatcctt aaaatcagat attaataata tcagtaaaga tactgatgtg attgctgtga
     6301 atcacttttc ggagtctgag cactttacga ctttgaaacc caataaatat attttgattg
     6361 atagctattt ttgggatgaa gccactcata gttcgttggt ggaaaaaaga gataaattgt
     6421 ttaaagatct aaacgaactt gtgtcttgga aaatgcaatt gattattcct gactttgctg
     6481 acatagcgtt cattcaggga aatatcaaaa acagcaacat agctgtaaaa gtgcttcgag
     6541 caattcctag taatgtaacc aatattaaat atgggccaat agcattatct tcaggtattg
     6601 ttgcacctcc agctgtaaat gttcttatct ttgctgtgta tgtagcaatt ttatgtgaat
     6661 atgattcagt ggaaatattt ggggcagata tgagcttcca taatgatgtc catgtcgatc
     6721 aaaaaaataa ccagctttat atggtctaca agcatttcta tggaaaagaa gagaagttgc
     6781 cgctcctaga aaatcctcaa aaggtcaaac cttttaatat gacggacttg atgcgattaa
     6841 ctttcttgac gttttatgca catcaactgt taaataaatg ggccgatctc aaaggggtaa
     6901 aaatcgtgaa taggtctagt ttctctatga tcgatgctta tcctagacga tgaaatgaca
     6961 ggtaaataga cgctacaatt gtagtgtcta tttaccgatg atcatttatt tttctgtaac
     7021 cacatagatt cagcgaaagt aaagtcctct ggccaatcta tatcaattga gctctctgca
     7081 tcctgtatgt acatcgaagg ctttagtcca attcgatcat ttagcttttt gtagttttct
     7141 acactattta aaaagactga gctatcgatt tcataaagag gttcaattgt ctgagttcta
     7201 ggccattttt ctatattacg atcgtagcta attggcttat ctttattcca aataaaccca
     7261 cgtagttctt tgaccgtcat taatgaatca taaccttccg tgagtgcttg attatactgt
     7321 ttgataatag cgctataacc tgactcgtct aagaatggtg aggttacatg agtccaaagt
     7381 acgtgacccg tagtaataat cgaaggaaca tatctaataa gatcatcagt gcttgttgaa
     7441 cttgagccta agtgctctgg gcgttcatct attcgtatgc gagaatcttt atatgttgaa
     7501 gcaaactcta gaacaacatc gtcgtttgaa gatacgacta tcgaatctag gtcagttata
     7561 ttgattaact gatccagttt gatttttagc aacccacctt ttacaccagc aaagtctttg
     7621 gtatttttgt ttgggatacg ttcactgcct tttcggcatg gtaaaaagac atggacactc
     7681 atgcttacca actccttaga atattgaaac cttcactttt tgataagctt tcaagctctt
     7741 cttttacatt tgaaaaagga agatagccaa gaaaatcaat attattctct ttggctgcgt
     7801 gaaagtctga taatgcgtca ccgatcatta gagccgcgcc tgaagactcc tttaggatgg
     7861 ttgcaacgtt atctgctttc gcggtaggtg atcctagaat taacgtaaaa tattgcgcca
     7921 gctttctttt aacaaatact tctcgtaatt cgtcttgagc agatccactc gctacataga
     7981 gtttggctgg gcaatctgta attgctacca taaaattagg cgtcagttga gctttcatgt
     8041 atagctcttc acactgcttt gagtagaagt ttagtatacg ttcgcacaat gtttcatctg
     8101 catttatgat gaaatgttca acaaagtaac tcacatgatg aaaacgcgat ttgccaaaat
     8161 tagctttgaa ataattgcaa caactgtcta tttcaagctc tgatacttgg gtcatgcgta
     8221 aagcattacg catggcatca attttaaggt tatttgagtc taggatgaca ccatcgcagt
     8281 caaaaataac agtatcatag ccattaaata gcgggtgcat agatagaccc tgcttttact
     8341 ggataagtgg taggggttaa cgcttgaatt gatgtacttc caaactgctg actaaccatg
     8401 ctaaagatct cattcatttc catttggcgt cggtcagtca catcataacc atcaaaacca
     8461 acgagagata ttttctgcgc ctgacctata aaagcgatac ataacgcata tgcggcggta
     8521 ttgtcatacg gtagcgtaca gtagttttta tgagcgacga gcgtatcctt ctctatagtg
     8581 ctactgtaat caatcataga aatattacta aatttgctta gctcgtcttc gtcaaagcgg
     8641 ttctttggca aaaacacggg cttggctacc tctgagtaat ggctgctttc tgacaagtat
     8701 ttagagttat gagatacagc aaagtaatcg aaaagctcag tgttaatctc atgctttgta
     8761 ttgatgccaa ttacgatagg ctttcgagtc ttaatgtatg tctcgatcgc gactctatgt
     8821 gttgctgtat tgggcgtatt agtgaccagt aaaatttctt ttcctgaaaa gatccctgat
     8881 agatctgatt ggctttcagt tggttggctt attttcccta cagtaagcgc ttcttctaat
     8941 acatcaccat tatagcttgt cgtaccttct agctttttta aatgctcgat agcaccaaca
     9001 atttcttctg ttccgtaatt aggattagaa agcatattct ggatataagt tgggtgaata
     9061 ttgtttttgg ctcccaagta atatagaagg cttgagcccc aaccgtatct tctttgcatt
     9121 ggctcaaaat ggcgaataac tagttcatat attgctgttg gcaagtattt atctagtcca
     9181 tcaagttcgg cgagcaggtt ttcggtctgg gcgttcccag cgcctcgtcc cattccggta
     9241 acggttacat ccaaccatgt aacaccattc gaacgagctg tcatcgtatt agacattgct
     9301 tgacccatgt tgttatgggt gtggatgcca atgtcgccat cccagttttg ctttacaatt
     9361 tcggcaatgc gagtgacttc tgcactatcc atattaccga gagagtcagc aaaatagatg
     9421 acgtcagggt ttgcttttgc taccgtctgt actttttccg ctataacctc ggatggtttt
     9481 ccgcccgctt gcatcagatt taaaccaacg atatacccca tttctttgaa agctttaatc
     9541 atgctttcac actgctctgc ttcattgaaa tgtgccgcga tacgaacgag atcaacttta
     9601 ctttcttgtg ctggtacaaa aagatcgttg atggctgctt caacaggttt attcgcatct
     9661 agcaacgttt ttgcgtcaac cattacgcca tatgtcggac ctttcggtag gtgtaacgtg
     9721 tttagaaact cttcagtcgt ataagcaaat ggaccactgt aaacagactt aggataatta
     9781 cgaagcccta attcaacata atcaatttta gcttttgcaa ctgcagtgag gtaagcgtca
     9841 acgacttctg gtgaaaagtc ccagtggtta tagtagccac catcgcgaag agtacagtct
     9901 aaaatttttt gcataatgga tattcttttt aagttaaaca ggtaatgtgc ttagtacagt
     9961 aaaacataca agcttaatag taaaagtgag gtatttatca aactaattat taagttatta
    10021 cttatcactt ttggggagta agctcattgt tcggtttaga gcgccttgat tggatgaggc
    10081 ccatttatta gcatgcaaag ctcggagtaa atatttatct tgttgagaaa tgatctctgt
    10141 agtccttagg gcaagggtcg ttgagtctgg cacaattaag ataccatcca cagctagtaa
    10201 gttttcaaca atctccttaa agttgaaata gctggggcca gtaatgatga ctttttctaa
    10261 catggcaggc tctagcacat tatgcccacc gaccttattt ccaactaggc ttccccccat
    10321 aaagcagatg tccgctgcgc ccatcaaagt tagcatctca cccattgtat cgcctagata
    10381 tacttgagta ttagtctcta ctgcatgcgt tgaagtacga cgcacagtgt taaagccttg
    10441 atgttggcaa agcgtgaata catcaccaaa tcgttctgga tggcgaggaa ccaaaacaag
    10501 taaagcattt ggatgagatt ttaaaacttg tctatgtgca tctaatacca gctcgtcttc
    10561 gcctttgtgg gtgctcgctg caatccatac tgggcgatct ttcccaagtt gcgctcgaag
    10621 tgcttcgcct tgttgcttca cgtgatctga aatttgaata tcaaatttaa tcgagcccgt
    10681 gacagatagc ttctctttat tgacgcctag gcgttcgaag cgctctgcat ctgaatctgt
    10741 ttggcagagt acttgagtta ggcatgggtg aagctggtta aaaagccgct gtatttttgc
    10801 gtagttatga cacgattttt cagagaggcg cgcattcacg acagttatag ggatacccac
    10861 tttgtgaaca ttgtgaagcg tgtttggcca cagttcagtc tcaatgatca acattttttt
    10921 tggttgtact gctttaagaa aacctttgat agcgaaccca aagtcaattg gcatatagcg
    10981 gtgttcaact aaattaccca acttagcaat ctgttcagct cccgtactcg ttgttgtggt
    11041 aactagaata gactgttcgg ggttttgttc tttaagtgct ttaattaaag gtgttgctgc
    11101 tatgctttcg ccaacagata cggcatgtat ccaaataggt ttgtcgttgc tttttagctt
    11161 gggagttatg ccaaagtgct ctctccagcg agagccgaac ttaggcttat ttggtttgct
    11221 cttgtacaaa ccaaataaaa gcagcggtga cgctagggcg agtagtaggg tataaacaat
    11281 acgaactaac atgagcttat cgtaattgcg tcaagttttt ggatactatc tagcacttgc
    11341 ttaggcgaaa gttctgtcaa gcacttaagg tgtttgtatt gacattcacg ctggaaacaa
    11401 gggcgacaat caatatcggt atgaacaatc ttcacttttt ctgcgagtgg gggtgtgtac
    11461 ttcggtgaag ttgatccata cactgcaaca acattacaac caaccgcagc agccacatgc
    11521 attaagcctg agtcattcgc gacaacagtc ttgcatgcag caagtagatc gaccgcttca
    11581 attaagctgg tttggcctgc tataacatga atgttctcat ggaattgtgt cgggacaagt
    11641 gcgcggatat tgttacaagt ctctaagtct ttttgagaac caaacaacca tatttgatgc
    11701 ccagatcgag ccatttgcgt cgcaacttca gcgtagtgtg tttcgggcca ttttttggca
    11761 ggaccaaact ctgctcctgg gcacagtcca atgattggtt ggtcttgcgt taagttgaac
    11821 tttttgagcg tggcttgttg ctcagtcgcg ttgattgcaa ggcgtgggcg aggtaaggtt
    11881 tcaagcccgc caagagaatt agagtccacc atttctactt ttggataagc caacgctaca
    11941 tagcgctcta ccatgtactg aaaagacttc atattggggc gtaagtcatt caataaacca
    12001 tagcgcattt ctcccttcca gccagtacga agaggaatgt tggcaaacca tgggatcagt
    12061 gctgattttg ctgatttagg caagatatag gcgtggtcat attgcttttc acgtaatgat
    12121 ttaccaattt ccctgcggcc taataagtta aactcaccat gaccgatagg catttcaatc
    12181 gcttggtgga tttctggcat acgctcaaga atcggtttac accatcctgg agcgataacg
    12241 tcaatttggc tatctggatg acgttgcttt aatacgatat ataacgattg cgacattacc
    12301 atatcgccga cccacgcagg gccaatgact aaaatcttca tgctttgttt ttccagtata
    12361 ggaatttcaa aattgactca taagttctaa aaagttcagc gtatattttg ttcgcaagag
    12421 tcattttgga tttgtctttt cttttcccac caatcgtcga agcaatctca gctctagtaa
    12481 aaaagtctgg agacacgctg taaacttgaa caccatgtct ccatggtttt tccatatagt
    12541 catcaactgg ttcaataaac tcttgagcat gcataacaaa ctttgaggct gctttaggtg
    12601 tgacgatata agcggtggta ccacaagtgc cagcagagta gccccctagc atatgattat
    12661 catcaatagg tacaattggg gtgaatgcac acttctgagt ggcagacaac ttgatgtagt
    12721 cataattaga agtcagtgga agggtatgtt cgagtacttg cttcagccct tctacggggt
    12781 cgacgttgtc ttctaagatg acgatcggtt catcaagctc aatacagcga acccacaact
    12841 cgtaatggct tgcaaagcag gcgacttcac tggtttttaa ttgataccct ttgcggcgta
    12901 gagtaatggt tggacgactt ttttctgaat gaagaaaact gtcaacagag ccatctacgg
    12961 catcaaaaaa ttcaaacgta atgccttgtt gttttaactt ttcttcaata cgttctcggc
    13021 gatctagcga tcgagccaag cttacgacaa atactttcac tgcactttcc agtcgctgtt
    13081 atatcttggt ttaaacaaat tgtaatagtt attgccgtct tcttgagagt gcagtaactg
    13141 tagtacccac atatattgtt cgggtttatc accgacaaaa tattcaatgg cttcattaca
    13201 ggtacgagcg tcttgctctt cgcctttatc cagctcaaac gccggtaaaa tctcaatatc
    13261 aaacgtacca tcttcagtgt tcatcgacgc aaatagaggc agtacttttg aacgtgatag
    13321 tttagcaatt ttacccaatc cagggagcgt tgctttacgt gtggcgaaaa agtcgacaaa
    13381 tacgctgtgt tcagggccat gatcttgatc gggtaggtaa tacccaatgt agccgtctcg
    13441 aatagatttt agaaaaggct taattccacc agatcgctcg taaactcggc cgccatactg
    13501 aactctttgc ttgtgcatta accagtcagc cacatcattc ttttgttttt ttgccatcgc
    13561 agagacaggc atccccattg aagccagcaa aatagcagga acatcgatag cccacgagtg
    13621 aggcaccatc aaaatcacat tttcattgtt atcaatgtgc tggcgtagta cgtccaatcc
    13681 tcgaatttgg cagcgactct gaagccattg aggagaacgc aaactcagca acccaaaacg
    13741 aaaaaagaac atagctgcgg tgtagagcgt tttttcaatt aaggcttcgc gttcctgaaa
    13801 cgtctttcca gggaagcata attcaaagtt gactcgagcc ctgtgagctg gccctttatg
    13861 agatttagat aactttttag ctattgtttt ggctagccac ttttgggcgc taagaggcat
    13921 taaggctagc ggcaaaaaga gtattacacc taaccaagtt ccccaatact taggcgacaa
    13981 aaaatgacgc tcaaattcag ggttatatgc tttggagtcg tagtcgtcac gttttgctgt
    14041 catggcgtgt ctagttacct atccgtcttg atttactcgt atgagagcaa ggtgataaaa
    14101 ataagatagt agagatgata caggctattg gaatggagta aaggtggcaa caagtgatgc
    14161 taccacctaa atgatactta actgttttgg atagttaagt attcggcaac gccttctgca
    14221 accgtcttga actcaacatc gcaaccggca gcgcgtagct tggtaaggtc tgcttgcgtg
    14281 aactcttgat aggcaccttt tagatgctct gggaaaggaa tagtttcaat ctcgcctttt
    14341 ccatgatggg caatgaccgc ttttgctact tcttcaaaag attctgcacg accagtacca
    14401 cagttgaaga tgccagaaac accattttca aggaaccaca ggtttacttt acatacatca
    14461 ccaacgtaaa cgaaatcacg cttaaaagac tcgctacctg caaacagttt tggattttca
    14521 cctgcgttta gttggttgtt cagatggaat gcaacagatg ccatgctgcc tttgtgatct
    14581 tcacgtgggc catatacgtt gaagtaacgg aagccggtga tttgagaaag ggtctcacca
    14641 tgttcttcag cgtcttgcca tagacgacga acatagttgt caaactgttg tttcgagtaa
    14701 ccgtatacat tcagtgcgcc ttcatattcg cgttcttcaa cgaaagtatc tgtatcgccg
    14761 taagtggctg cggaagaggc gtatagaaat ggaatttcac gatctaaaca gtaatgaagc
    14821 agctctttag agtactcgta attgtttagc atcatgtatt taccatccca ctctgtcgtt
    14881 gcagagcaag cgccttcgtg gaaaattgct tcgattggac cgaaatcgtc accagccata
    14941 atttgggtca gaaagtcgtc acgatccatg taatctgtga tgtccagatc aactaagttc
    15001 ttaaactttt tgccattttt taggttgtcg acaaccagga tatcgttaat accagcttcg
    15061 tttagtgctt taacaatgtt gctgccgatc atgccagcac caccagtaaa aagaatcaag
    15121 a
//
