LOCUS       JQ863081               15663 bp    DNA     linear   BCT 11-NOV-2012
DEFINITION  Vibrio parahaemolyticus strain 20578 serogroup O8 O serogroup
            genetic determinant region genomic sequence.
ACCESSION   JQ863081
VERSION     JQ863081.1
KEYWORDS    .
SOURCE      Vibrio parahaemolyticus
  ORGANISM  Vibrio parahaemolyticus
            Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
            Vibrionaceae; Vibrio.
REFERENCE   1  (bases 1 to 15663)
  AUTHORS   Chen,M., Guo,D., Wong,H.C., Zhang,X., Liu,F., Chen,H., Chen,M.,
            Liu,B., Wang,L., Wu,F. and Feng,L.
  TITLE     Development of O-serogroup specific PCR assay for detection and
            identification of Vibrio parahaemolyticus
  JOURNAL   Int. J. Food Microbiol. 159 (2), 122-129 (2012)
   PUBMED   23072697
REFERENCE   2  (bases 1 to 15663)
  AUTHORS   Chen,M., Guo,D., Wong,H.C., Zhang,X., Liu,F., Chen,H., Chen,M.,
            Liu,B., Wang,L., Wu,F. and Feng,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2012) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..15663
                     /organism="Vibrio parahaemolyticus"
                     /mol_type="genomic DNA"
                     /strain="20578"
                     /db_xref="taxon:670"
                     /note="serogroup: O8"
     misc_feature    1..15663
                     /note="O serogroup genetic determinant region"
     gene            415..1125
                     /gene="kdkA"
     CDS             415..1125
                     /gene="kdkA"
                     /note="KdkA"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="AFV93029.1"
                     /translation="MIQQYRDSNQVIWFDEALIEDPSQPIFDAEYWQSTNKVTGSASG
                     RGTTWFVQLDTMQAALRHYRRGGLFGKLVKDNYWFSGWEQTRCAQEFQLLLTLINAGV
                     HVPRPIAARAVKSGLTYQADLLSERIPNARDLVSILQEKPLPEGMYQKIGQEIAKMHN
                     AGVNHTDLNIHNILIDDKDKVWIIDFDKCRKQEHGDWKKQNLERLLRSFKKELLKRQI
                     IWQASDFDHVQKSHTENT"
     gene            1218..2282
                     /gene="rmlB"
     CDS             1218..2282
                     /gene="rmlB"
                     /note="RmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-D-glucose 4,6-dehydratase"
                     /protein_id="AFV93030.1"
                     /translation="MKILVTGGAGFIGSAVVRHIIRDTQDSVVNLDKLTYAGNLESLV
                     DVADSDRYYFEQVDICDRTELDRVFSEHQPDMVMHLAAESHVDRSIDGPAAFIETNAM
                     GTYHLLEAARQYWSSLEEANKSAFRFHHISTDEVYGDLEGTDDLFTETTSYAPSSPYS
                     ASKASSDHLVRAWQRTYGLPTLVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGD
                     GMQIRDWLFVEDHARALYKVVTEGEIGETYNIGGHNEKANIEVVKTICALLEELRPDK
                     PAGVESYESLITYVKDRPGHDVRYAIDATKIAQELNWTPEETFESGIRKTVEWYLNNP
                     QWWQRVLDGSYSLERLGAGE"
     gene            2282..3154
                     /gene="rmlA"
     CDS             2282..3154
                     /gene="rmlA"
                     /note="RmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AFV93031.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIREILIITTPEDQEGFQRLLGDGSEFGIELQYAIQPSPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIFYGQGFTPILQQAAMKSRGATVFGYQVKDPERFGVVEFDANMQAISIEEK
                     PVKPKSNYAVTGLYFYDNRVVELAKKVKPSARGELEISTLNQMYLEQGDLNVQLLGRG
                     FAWLDTGTHESLHEASSFVQTIENVQGLKVACLEEIAWRNGWLTKDQLLAIAKPMQKN
                     DYGRYLSELVIEQK"
     gene            3185..4273
                     /gene="wvaD"
     CDS             3185..4273
                     /gene="wvaD"
                     /note="WvaD"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase"
                     /protein_id="AFV93032.1"
                     /translation="MMSSEIIPVTKPYLPSKDKYKQYVDRVYESGWLTNSGPLVNELE
                     SRLAEYLGVKHVILVANGSLALQVAYKALNLTGEVITTPFSFAATTSTLSWENLTPVF
                     CDIENDSFNINPDLIEALITEKTSAIVPVHVFGNPCKVNEIQAIANKHGLKVIYDAAH
                     AFGVDLNKESVLTYGDISTLSFHATKLFHTIEGGAVITQCDELAAKVRSMINFGITGP
                     DSIEGVGTNAKMNEFEAAMGHCVLDDIESIMTKRRGLWQSYQTHLSGVFQMQNWLDSS
                     KPNGAYVPIVFDSEQSLLRAKANLEANNIFPRRYFYPSLDLVDSYGHEGTPCEVSRST
                     SKRILCLPIYFDLCESMVNKIKQILKKS"
     gene            4298..5833
                     /gene="wvdA"
     CDS             4298..5833
                     /gene="wvdA"
                     /note="WvdA"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase and ligase"
                     /protein_id="AFV93033.1"
                     /translation="MRNSILKYLQASSMKHPENICVSDSSHSLTYAESTAKSYAISLQ
                     LRQQGSLNKPIVVFAKKSVNALSMIFSATMAGGIYCPVDINSPKERLLKILSNLQDCL
                     VLYDETSGNLLDSLELDSSISTINIDEIPASKLPTQDIWADIEKFNKTVIDKDPCYII
                     FTSGSTGTPKGVTICHSSVIDYIDWANSAYEVSKVDIIGSQAPLFFDNSTLDIYLALS
                     NAAELHVIPESVFIFPQKTLEYLKEKAITTIFWVPSLLVNIANLKLLDQFQLPCLRNV
                     LFAGEVMPAKTIKYWLDKHPNAHYSNLYGPTEITVDCTYYDVPSDWDGDSLPIGIPCH
                     NSGILILDENDQRNDEGELCVRGSSLALGYWANSEKTEEVFCQNPLQDKYIDMIYRTG
                     DLVRLVDGLIYYIGRKDFQIKHNGYRIELGEIESQIMELGAVEQCVVGYLSEDKVLYS
                     FVQCSSSMSEVELKVALTKRLPKYMIPSKIGFVQEMLFTPNGKLNRKAFSEKALQEFV
                     KVK"
     gene            6069..7136
                     /gene="wvdB"
     CDS             6069..7136
                     /gene="wvdB"
                     /note="WvdB"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase"
                     /protein_id="AFV93034.1"
                     /translation="MKSDIGNISLKGIVAAVPKHISYFDDEIKNYSHTEQSSKKLKKL
                     MGYNQHHVVSKETALTDMIFPLLEDLSTTLGCNYQDVDGILVVTQTPDYPIPSTASIV
                     HGAFDFKTDCYCMDINDGCNGYIRGLFEASSLVRNSDAQKVLLIAGDVLSRKVSLKDR
                     NSYPLIGDAVTITLIERRAENVKAPLEIKHDGKGAKALMIPSGGMVNPCTSDTATLAE
                     DEEGNWRCSEHLVMQGRDVFTFTQTTVINFIKEFIETNTKPEEIDYFFFHQANKFILD
                     RFRSALKVTEEQLPSEVVTIYGNSSSATVPMSILSSWMKNSGFEGKKALLSGFGVGLS
                     WGATLVDVNELTLCKLIKLDV"
     gene            7358..8098
                     /gene="wvdC"
     CDS             7358..8098
                     /gene="wvdC"
                     /note="WvdC"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="AFV93035.1"
                     /translation="MKRKVLITGATSDIGLSIAHKHVQEGNFVILLGRNEESLKIAEE
                     SLGKDSSAAYVWDLKSPSSLKPLLKKIVTEQGKVTDLICAAGYHKILPLKLVSDEELL
                     LTMQTNVNSHLLLCQLFSNALYSDKSQDRSITLISSIAHFVGEPGLVAYSAAKGALIS
                     ASKSMARELAPRKIRVNTVSPGWVETKHTKDVAVDLGEAALEKLHNLYPLGLGKPEDV
                     AEACYFLSSRASRWVTGVDLVIDGGRSV"
     gene            8205..8966
                     /gene="wvdD"
     CDS             8205..8966
                     /gene="wvdD"
                     /note="WvdD"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="AFV93036.1"
                     /translation="MQPFSTLESFAQKHDVAYQFKHTKEEIESSLLGWLSGNTLIISA
                     NNNYLFKGKLLTLKNVTIINFHNALLPNLRGRNAPSWAIFHQLEKTGVTWHYVDEGVD
                     TGEAIAFLDVLVDKHDKAYQVAHKCMHAGLEAFSNVIDELLQTGSTPCLTKQPLRYDY
                     LYLSKDIPGNGWLDIKQSTLENYRILRALDYGPFKVFPKALVDNGGDKYSVRAYQFID
                     EPSDKSVNSSENSIVINDEFGLLKLQVEKVKIELR"
     gene            8970..9602
                     /gene="wvdE"
     CDS             8970..9602
                     /gene="wvdE"
                     /note="WvdE"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acyltransferase, sialic acid
                     O-acetyltransferase NeuD family"
                     /protein_id="AFV93037.1"
                     /translation="MSNPIIIFGASGLAAEVYSWIQDVNDIRERFLCFCVTDEYFISG
                     TTFYGHEVKSISMLNDIEQFDFILAFGGEGYARASIAETLKNKGGNAISFIHPSVVLS
                     PFALIGEGVIINPNCSISPGVVIGDFSLINCNCAIGHDVKIGRYTSLLGSDTINGYVE
                     IGERVLIGSGATIHPKVKVADDATVGMGAVVFTKVRSNTTVLGNPAKKIS"
     gene            9511..10497
                     /gene="wvdF"
     CDS             9511..10497
                     /gene="wvdF"
                     /note="WvdF"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFV93038.1"
                     /translation="MMRQLAWGLSYLPKFALIRRCLVIQLKKFLSLSINILGCFNQIR
                     IAMKKKVSVLCISYNHEKYLKNALDGILNQVTEFDYELIIYDDYSLDKSRDVIQSIVS
                     AYKGKCEIKLVFPSENQYSKGFTVIPFMLPYVEGEYIAICEGDDYWIDNNKLQKQYES
                     LKHDHSVDICFHPSFTLLDGNIQDKGYGYLEEEGKKIDYSQVVMKGGSMMPMASIFFR
                     AQPLLDIVSKNEEFFKRNFYHSILQTVMVLGKGALYIPDKMSVYRSMHEGSWTLQQSL
                     DKEKREREFKASNKRLWELNQIFNNKYLGLFLQFYYKRNVKFYKDKLKSIYK"
     gene            10503..11345
                     /gene="wvdG"
     CDS             10503..11345
                     /gene="wvdG"
                     /note="WvdG"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthan biosynthesis pyruvyltransferase"
                     /protein_id="AFV93039.1"
                     /translation="MVKNYKGFRGFVKRLSYKYDKSQEVDATKEVALHWWNGRVNFGD
                     VVNKFIVEQLSGKSAIWSSDKSDKEHYLVIGSVLQSSNDNAIVWGSGIISDRKTPLFK
                     PKKVHAVRGPKTRDILIARGIECPEIYGDPALVLPTLIQPNEAESAYKLGIIPHYNNK
                     NDAFFKQNFPDDVKVIDIETDDVQAFIDDIASCELIVSSSLHGIIIADAYGVPAHHIS
                     FDDTVEGGEFKFFDYYLSVGRECNLPIKVSSSTSIDELCELPKNYDINIDIQPLLDSC
                     PFKQ"
     gene            complement(11385..12107)
                     /gene="wvaH"
     CDS             complement(11385..12107)
                     /gene="wvaH"
                     /note="WvaH"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFV93040.1"
                     /translation="MKVFVINLKRSVDRRVRMENALNKANIPFEFIEAIDSSTPNFLY
                     SERSNNALTRKRFGYRLVENEIACFSSHHLAWEKCLEVNEPILVLEDNCDLLPEFFDV
                     LSHFDTLAEQYDFLKLAATKPKSFKVISSVNNKLNIVRFNKRTCGIMGYILTPHAASK
                     FIKNATQFIEPVDDYMEKPYKHGVSTYVLRPDIVKRADIPSTIGSSRKDKSDINLCNK
                     IYIELFRLYEQIQDRLVVHRDK"
     gene            complement(12104..13375)
                     /gene="kdtA"
     CDS             complement(12104..13375)
                     /gene="kdtA"
                     /note="KdtA"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="AFV93041.1"
                     /translation="MLIRIIYTALLALASPFLLLGLYKSKPNKPKFGGRWKEHFGITP
                     QLKTHQRPIWIHAVSVGESIAATPLIKELKQQYPEQPIVVTTTTSTGAEQIAKLGDLV
                     EHRYMPIDFAFAVKSFLKAIQPKKMLIIETELWPNTLNVVKQANIPITVVNARLSEKS
                     CQNYAKVQWLFNQLHPCLTQVLCQTDSDAERFERLGVNKEKLSVTGSIKFDIQISDHV
                     KQQGKALRAQLGKDRPVWIAASTHKGEDEQVLEAHKQILESHPNALLILVPRHPERFD
                     DVFALCKKQGFETVRRTEKQPAENTTQIYLGDTMGEMLVLIDAADICFMGGSLIGDKV
                     GGHNVLEPAALGVPVITGPSYYNFQDLVAKMLHKKVIKLIESPNEMSLEVEAMFQDAL
                     AKRVVQENLLSFVKDNVGALTKTIRCIESIK"
     gene            complement(13369..14427)
                     /gene="waaF"
     CDS             complement(13369..14427)
                     /gene="waaF"
                     /note="WaaF"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose-LPS heptosyltransferase II"
                     /protein_id="AFV93042.1"
                     /translation="MKKILIIGPAWVGDMVMSQSLYITLKQLHPESQIDVIAPGWCKP
                     ILERMPEIHQAIEMPIGHGEFNLLGRREIGKSLREKQYDHAYILPKSAKSALIPWFAN
                     IPLRTGWKGEMRYGLLNDLRPNMKSFQYMVERYVALAYSKSEMVDSSSLGGLDTLPRP
                     SLSINKEEQQTTINKFNLDQKRPAVGLCPGAEFGPAKKWPETHYAEVATQMCKAGHQV
                     WLFGSQKDLETCNNIRALVPTQFHEHIHVLAGQTSLIEAVDLLAACKTVVANDSGLMH
                     VAAAVGCNVVAVYGSTSPKYTPPLAEKVEMVHTDIDCRPCFKRECQYQHLKCLTELSP
                     KQVLDSIQKLEAIATSSC"
     gene            complement(14424..15413)
                     /gene="msbB"
     CDS             complement(14424..15413)
                     /gene="msbB"
                     /note="MsbB"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
                     acyltransferase"
                     /protein_id="AFV93043.1"
                     /translation="MTAKRDDYDSKAYNPEFERHFLSPKYWGTWLGVILFLPLALMPL
                     SAQKWLAKRIAKKLSKSHKGPAHRARVNFELCFPGKTFQEREALIEKTLYTAAMFFFR
                     FGLLSLRSPQWLQSRCQIRGLDVLRQHIDNNENVILMVPHSWAIDVPAILLASMGMPV
                     SAMAKKQKNDVADWLMHKQRVQYGGRVYERSGGIKPFLKSIRDGYIGYYLPDQDHGPE
                     HSVSVDFFATRKATLPGLGKIAKLSRSKVLPLFASMNTEDGTFDIEILPAFDLDKGEE
                     QDARTCNEAIEYFVGDKPEQYMWVLQLLHSQEDGNNYYNLFKPRYNTDWQVEK"
BASE COUNT     4587 a   2865 c   3462 g   4749 t
ORIGIN
        1 tgtcaaagac aacaccgcat caatcgcaag aatctggaaa gccaggcaat actggggtaa
       61 agcgcattat caaagcgaca ggttattcta tacagggatt aaaagccgct tttaaacatg
      121 aagctgctgt tcgacaagag ctcgctttgc taagcattgc tgtgattttg gcgtgcgtgg
      181 ttgatgtcgg gatgatagag cgcatcttat tgattggtgt cgtggtatta gttctgattg
      241 tcgaactgat taactccgcg atagaagcag tggtggatcg cattgggttg aacgccaaat
      301 taagtggccg cgctaaagat ataggatcgg cagccgttat ggttgcgttg gcgttcgcag
      361 cttttacttg gctatacata ttggccagtc gctacctagg ctagaggtta caacatgatt
      421 cagcagtatc gagatagcaa ccaagtcatt tggtttgatg aggcgcttat tgaagatcct
      481 agtcagccta tttttgatgc cgagtattgg caatccacca acaaagtaac tggcagtgcg
      541 tcagggcgtg gcacgacttg gtttgttcag cttgatacga tgcaagccgc tctgcgtcat
      601 tatcgacgtg gtggcttgtt tggtaagtta gttaaagaca attattggtt ttcaggttgg
      661 gagcaaacgc gttgtgcgca agagttccaa ctattactta cactgattaa tgctggcgtt
      721 catgtgccaa ggccaattgc tgcgagagct gtgaaatctg gattgacata tcaggccgat
      781 ttattgagtg agcgcattcc taacgcaaga gacctggtca gtatcttgca ggaaaaaccg
      841 ttaccggaag gaatgtatca aaaaataggg caagaaattg ccaaaatgca caacgctggc
      901 gttaatcaca cagacctgaa tatccacaac atcttgatcg atgacaaaga taaagtgtgg
      961 attattgatt ttgataagtg ccgcaaacaa gagcacggcg actggaaaaa gcaaaactta
     1021 gaaaggcttc ttcgctcatt caaaaaagaa ctgctcaaac gccaaatcat ttggcaagcg
     1081 tccgattttg atcatgtaca aaaaagtcac actgaaaata cgtaaagtga gctttttgtc
     1141 agttattcgt gagacaatcc aatagcgcat ttcggtacac ccactaaaag tacccattaa
     1201 agtaggtaac atttgcaatg aaaatactag taacaggcgg ggcaggcttc attggctcag
     1261 ctgtcgttcg acacattatt cgtgacaccc aagatagcgt cgtgaattta gacaagctca
     1321 cctacgcagg aaacttggag tcgttggtgg atgtcgcgga cagtgaccgt tattacttcg
     1381 agcaagtgga tatctgtgac cgaactgaac tagaccgcgt gttttcagag catcaacctg
     1441 atatggtgat gcatttagcg gctgagtcgc atgtcgatcg ctccattgat ggtcctgctg
     1501 cttttattga gaccaacgcc atgggcactt accatcttct tgaagcggcg cgtcaatatt
     1561 ggtcaagcct tgaggaagcg aacaaatcgg cgtttcgttt tcatcatatt tctacggatg
     1621 aagtgtatgg cgatttagag ggtacggatg atttattcac cgaaaccact tcatacgcgc
     1681 cgagcagccc atactcggcg tctaaagcgt cgagcgatca ccttgtacgc gcttggcagc
     1741 gtacttatgg tctcccaaca ctcgtaacta attgctcaaa caactatggt ccgtaccact
     1801 ttccagagaa gttgatccca ctgatgattc tgaatgccct cgatggcaag cctttacctg
     1861 tgtatggtga tggcatgcaa atccgagatt ggctgtttgt ggaagaccat gctcgcgcgc
     1921 tttacaaagt cgtgacggaa ggtgaaatcg gtgagaccta caacatcggt ggccataacg
     1981 aaaaagcaaa catcgaagta gtgaaaacca tttgtgcttt gcttgaagag ctgcgaccag
     2041 acaagccggc aggtgttgag tcttatgaat ctttgatcac ttatgtcaaa gatcgcccag
     2101 gtcatgatgt gcgctacgca attgatgcca ccaagattgc tcaagagctt aactggacgc
     2161 cagaagaaac ctttgaaagc ggcattcgca aaaccgtgga atggtacctc aataatccgc
     2221 aatggtggca acgcgtgctt gatggttcat acagtttaga gcgattagga gcgggcgaat
     2281 aatgaaaggc attattttag caggtggctc gggcactcgc ctttacccta ttacacgcgg
     2341 tgtctctaag cagttactgc cggtttatga caaaccaatg atttactacc cgttgtcggt
     2401 actcatgctt gctggcattc gtgaaatctt gatcatcacc acgccagaag accaggaagg
     2461 atttcagcgc ctattgggtg atggctcaga gtttggtatt gagttgcaat acgcgattca
     2521 accaagtcca gatggcttag ctcaagcttt catcataggt gaagagttca ttggcgatga
     2581 ctcagtatgt ttagtgctgg gtgacaacat cttttatggg caaggattca ctcctattct
     2641 tcagcaagct gcgatgaaga gccgtggtgc gactgtattc ggttatcagg ttaaagatcc
     2701 tgaacgattt ggcgttgtag aatttgatgc caatatgcag gcgatttcga ttgaagaaaa
     2761 accagtaaag cctaaatcca attatgctgt cactgggctg tatttttatg ataaccgagt
     2821 cgtcgaattg gcaaaaaaag tcaaaccttc agctcgtggt gagttagaga tttcgacgct
     2881 aaaccaaatg taccttgagc aaggtgacct gaatgttcaa ttactagggc gcggttttgc
     2941 ttggttagat actggaacgc atgaaagcct acatgaagcc tcttcgtttg ttcaaaccat
     3001 tgaaaatgtt caaggtctga aagttgcgtg tctagaagag attgcatggc gcaatgggtg
     3061 gttaacaaaa gatcaactac tcgctattgc gaaaccaatg caaaaaaatg attacgggcg
     3121 ttacctttcc gagttagtga ttgagcaaaa ataatattgg tcgctaattg caatgagata
     3181 ttaaatgatg agcagtgaaa ttattcctgt aacaaaacca tatttaccat caaaagataa
     3241 atataaacaa tatgttgatc gggtgtacga gagtggttgg ttgaccaata gtggtcctct
     3301 ggttaatgag ttagaatctc gactagctga ataccttggt gttaagcatg ttatattagt
     3361 tgcaaatggc tcattagcac ttcaggttgc ttataaagca ttaaatctta caggagaggt
     3421 gatcacaaca cctttctctt ttgctgcgac tacaagcacg cttagttggg aaaacttaac
     3481 acctgttttt tgcgatattg aaaatgatag cttcaatatt aaccctgatt tgatagaagc
     3541 ccttattaca gaaaaaacgt ctgcaatagt accagtacac gtgtttggta atccatgtaa
     3601 agtcaatgag attcaagcta tagcaaacaa gcatggttta aaagttatct atgatgccgc
     3661 tcatgctttt ggtgtggatc taaataaaga aagtgtatta acgtacggtg atatcagtac
     3721 attaagtttc catgctacaa agttattcca cacgatagag ggtggtgctg tgattacgca
     3781 atgtgatgag ctagcagcta aagtacgctc tatgattaac tttggaataa cgggaccgga
     3841 ttcgatagaa ggcgttggaa ctaatgccaa gatgaatgag tttgaagccg ctatgggtca
     3901 ctgtgttcta gatgatatag aaagtatcat gaccaagcga agaggcttat ggcaaagtta
     3961 tcaaactcac ctttctggtg ttttccaaat gcaaaattgg ttggatagct caaaacctaa
     4021 tggggcgtat gtaccaattg tgtttgattc ggagcagtca ttacttagag ctaaggctaa
     4081 tttggaagcg aataacatct ttccaagaag gtacttttat ccaagtttag atcttgttga
     4141 tagttatggt catgaaggaa ctccatgtga agttagtcgc tcgactagta aaaggatctt
     4201 atgcttgcct atatattttg atttgtgtga atcaatggta aacaagataa aacaaatact
     4261 taaaaaatca tgattggttg caaaaagaga gtataagatg agaaacagca tcctgaaata
     4321 tctacaagca tcgtcgatga aacacccaga aaatatttgt gtttcggatt cgagccactc
     4381 tttgacttat gcagaaagta cagcaaagtc atatgctatc tcattgcaac taagacagca
     4441 gggaagttta aacaagccga ttgttgtgtt tgcgaaaaaa tctgtaaatg cactctccat
     4501 gatcttttct gcgacaatgg ctggagggat ttactgtccc gtagacatta actcacctaa
     4561 agaacgacta ttaaagatct tatctaacct tcaagattgt cttgttcttt atgatgaaac
     4621 gtcaggaaat ttattagatt cattagaatt agatagttca atttcaacta ttaatatcga
     4681 tgaaataccg gcatctaagc tacctacgca agatatttgg gctgatatcg aaaagtttaa
     4741 taaaaccgtt attgataaag atccatgtta tataattttt acttcaggtt ctactggtac
     4801 accaaaaggt gtcactattt gccactctag tgttattgat tatattgatt gggctaattc
     4861 agcctatgaa gtgagcaaag ttgatattat tggtagtcaa gcaccactat tctttgataa
     4921 ctcgacttta gacatctatt tggcgttgtc taatgcggca gaactgcatg ttatccctga
     4981 aagcgtgttt atttttcctc aaaaaacact tgaatattta aaagaaaaag caataacaac
     5041 gatcttctgg gttccttcat tgcttgtaaa tattgcgaat cttaagttac ttgatcagtt
     5101 tcaattgcca tgcttgagga atgtattgtt tgctggggaa gtaatgccag cgaagacgat
     5161 taagtactgg ttagataagc atcctaatgc tcattactct aacttatacg gaccaacaga
     5221 aattaccgtt gattgcacct attatgatgt gccgtcagac tgggacgggg atagcctacc
     5281 tatagggatc ccttgccata actcaggcat tttaatccta gatgaaaacg accagcgtaa
     5341 tgatgaaggt gaattgtgcg ttcgaggctc tagcttagcg cttggttatt gggctaactc
     5401 agaaaaaaca gaagaggtat tttgtcaaaa ccctcttcaa gataaataca ttgatatgat
     5461 ctatcgtacc ggagacttgg ttagacttgt tgatggttta atttattata ttggtagaaa
     5521 agacttccag attaagcaca atggctatcg tatagaactt ggtgaaattg agtctcagat
     5581 catggagcta ggtgctgtcg aacagtgtgt tgttggttat ttatcagaag ataaagtgct
     5641 ttattctttt gttcaatgca gtagttcgat gagtgaagtt gagcttaaag ttgcactgac
     5701 gaaacgttta ccaaagtaca tgattccaag caagattggc tttgtacaag aaatgctttt
     5761 tacgccaaat ggtaagttaa atagaaaagc tttttcagag aaagcgctac aagaatttgt
     5821 aaaggttaaa tagtagtatg tgtgaaaaaa ttatagcaat cctaaccaca atgaaaccag
     5881 agcatgagtt tgctaaagat actgaacttt ttgaatcagg tctgttggac tcttttgatt
     5941 tgttagtcct agtaagtgag atcgaagctc agctagctgt ttcaatccct ggtgaaggtt
     6001 tggttcctga gaattttgtt tcgccgcatg ttattggtga gtatcttaaa acgattcaag
     6061 gttaatttat gaaaagtgat attggcaata tctcgctgaa agggatagtc gcagccgttc
     6121 caaagcatat ttcttacttt gatgatgaga taaaaaacta ctcacacaca gagcagagtt
     6181 ctaaaaagct gaagaagcta atgggataca atcaacatca tgttgtttca aaagaaacgg
     6241 cgctcactga tatgattttt cctttgcttg aggacctgtc aaccactctt gggtgcaact
     6301 atcaggatgt ggatggaatt ttggtggtaa ctcaaactcc tgattaccca attccgtcaa
     6361 cggcttcgat agtacatggc gcttttgact ttaaaaccga ttgttattgc atggacatca
     6421 acgatggttg taatggctat attcgaggtt tgtttgaagc ttcgagccta gttcgtaata
     6481 gtgatgcaca aaaagttttg ctaattgctg gtgatgtttt aagccgtaag gtttctttaa
     6541 aagatcgtaa ctcataccct cttattgggg atgcagttac tatcacttta atagaaagaa
     6601 gagcggaaaa tgttaaggcc cctcttgaga tcaaacatga tggtaaagga gcaaaagctt
     6661 taatgatccc atcaggtggt atggtaaacc cttgtacctc tgatactgcc actcttgctg
     6721 aggacgaaga agggaattgg cgttgttctg agcatttggt catgcaaggt agagacgttt
     6781 ttacgtttac tcaaacaacg gtaatcaatt ttattaaaga gtttattgaa acgaacacaa
     6841 aaccagaaga gattgactac tttttctttc atcaagcaaa taagtttatc ttggatcgtt
     6901 tccgttcagc actgaaggta acggaagagc agttaccttc agaggtagtg actatttatg
     6961 gtaactcttc gtccgcaact gttccaatgt cgattttatc atcttggatg aagaattcgg
     7021 gctttgaggg taaaaaagca ctattatcag gctttggtgt aggtctgagt tggggggcaa
     7081 ccttggttga tgtcaatgaa cttacactat gtaaactaat taagttggat gtataaatga
     7141 ctgttttttt ggaagaacta gctgagatcc tagagttgga agtagaagag ttaactcctg
     7201 attttgaata tcaagaaacg gaagagtggg actctttagc ccaattatct cttatcactt
     7261 tattcgcaga tgaatatcaa gtagaaattg ggcacggtga tttaatcgaa cgaaaaactg
     7321 tgggtcaatt gctcgagcta cttcctcagt aatttcgatg aaaagaaaag ttcttatcac
     7381 aggagcgact tcagacattg gcttgagtat tgctcacaaa catgttcaag agggaaactt
     7441 tgtgatcctt cttggacgta atgaagagag ccttaaaatc gctgaggaat ctttaggaaa
     7501 agattcctca gccgcttatg tttgggattt gaagtctcct tcatcattaa aaccgctgct
     7561 gaaaaagatc gttactgagc aaggtaaagt tacagatctt atctgtgctg cgggctacca
     7621 taaaatcttg ccgttgaaat tagtatcgga tgaagagctt cttttgacta tgcaaacgaa
     7681 cgtgaatagt cacttgctcc tttgccagct tttttcgaac gctttatatt cagataagtc
     7741 tcaggataga agtattacgc tgatttctag cattgcccat ttcgttggtg agccaggatt
     7801 agttgcttat tctgcagcaa aaggtgcatt gatttctgca agtaaatcaa tggctagaga
     7861 attagctccg cgtaaaatca gagtaaatac cgtttcgcct ggatgggttg agaccaagca
     7921 cacaaaagat gtagcggttg atttgggcga agcagcactt gaaaagttac ataacctata
     7981 tcctttaggt ttaggcaaac ctgaagatgt tgcagaggcc tgttattttt tgagtagcag
     8041 agcgtctcgc tgggtgactg gtgtggatct tgtgattgac ggagggcgca gtgtctaaat
     8101 taaagtttga aaccgtaatc gtgatcggtt ttggtaagat agctcaagac gttgcttcca
     8161 agcttcttca atatgatgtg acgctgaaat tgttacagac agagatgcaa cctttttcaa
     8221 ctcttgagtc atttgctcaa aaacacgatg tcgcatatca atttaagcat acgaaagaag
     8281 aaattgagtc ttcgttactc gggtggctgt ctggtaatac gttaatcatc agtgcgaata
     8341 ataactacct ttttaaaggg aagttactga ctttaaagaa tgtaactatt atcaattttc
     8401 ataatgcact tttaccaaat ttacgaggaa gaaacgcacc tagttgggct atttttcatc
     8461 aattagaaaa aacaggtgtc acttggcatt atgttgatga aggtgtagac actggtgagg
     8521 ctattgcttt tttggacgtg ttagttgata agcatgacaa agcatatcag gtcgctcata
     8581 aatgtatgca tgccggacta gaagcctttt caaacgtaat tgatgaattg ttgcaaacag
     8641 gaagtactcc ttgcttgact aaacaaccat tgaggtatga ctatctgtat ctgtccaaag
     8701 atatccccgg aaatggctgg ctagatataa aacaatcaac gcttgagaac tatcgcatat
     8761 tgagagcatt agattatgga ccatttaaag tttttcctaa ggctctagtt gataatggtg
     8821 gtgataaata ctctgtacgt gcttatcagt ttattgatga accaagcgat aaatctgtaa
     8881 atagtagcga aaatagtatc gtgataaatg atgagtttgg cttgttaaag ttacaagttg
     8941 aaaaagtcaa aattgagctg agataaataa tgtctaatcc aattattata tttggagcta
     9001 gtgggctagc tgcggaagtg tacagctgga tacaagatgt aaatgatatt cgtgaacgtt
     9061 ttttatgttt ttgtgtgact gacgaatatt ttatatcagg aacaacattt tatggtcatg
     9121 aagtaaaatc aatctccatg ctgaatgata ttgaacagtt tgattttatt ttggcgtttg
     9181 gaggggaggg ttatgcccgc gctagtattg ctgaaacgct aaaaaacaaa ggtggcaatg
     9241 ctatctcgtt tattcatcct agtgtggtct tgagcccgtt cgctttaata ggtgaaggtg
     9301 taatcattaa ccctaattgc agcatctctc ctggtgtagt tataggtgat ttttctctta
     9361 tcaactgcaa ttgtgctatt ggacatgacg tcaaaattgg tcgctatacc tctttattag
     9421 gaagcgacac tatcaatggt tatgtagaaa ttggtgagcg agtattgatt ggctcaggag
     9481 caactatcca ccctaaagtc aaagtagcag atgatgcgac agttggcatg ggggctgtcg
     9541 tatttaccaa agttcgctct aatacgacgg tgcttggtaa tccagctaaa aaaatttctt
     9601 agtctatcaa taaatatact tggttgcttc aatcagataa ggatagcaat gaaaaaaaaa
     9661 gtttcggtac tatgtatttc ttacaatcac gagaaatatt taaaaaatgc attggatggc
     9721 atacttaatc aagttacaga gttcgattat gagttgatca tatatgatga ttactcgtta
     9781 gataaaagtc gtgatgttat tcaatctatt gttagtgctt ataaaggtaa gtgtgaaata
     9841 aaattagtgt tcccgagtga gaaccagtat tcaaaaggat ttactgtaat tccttttatg
     9901 cttccgtacg tagaaggtga gtatattgcc atttgtgagg gtgatgatta ttggattgat
     9961 aacaataagc ttcagaaaca atatgagagc cttaaacacg atcactctgt ggatatttgt
    10021 ttccatccgt cttttacttt attggatgga aatattcaag ataaaggata tggctatctt
    10081 gaagaagagg gtaaaaaaat agattacagt caagttgtaa tgaagggtgg cagcatgatg
    10141 cctatggcgt caattttttt cagagctcag ccgttgcttg atattgtttc gaaaaatgaa
    10201 gagtttttta agagaaattt ttaccattca atattacaga ctgtaatggt tttaggcaaa
    10261 ggagcactat atattccgga caaaatgtcc gtatatcgaa gtatgcatga aggctcttgg
    10321 acgttacaac aaagtttaga taaagagaag cgagagagag agtttaaggc atcaaataaa
    10381 aggttatggg aattaaatca gatattcaac aacaaatacc tgggattgtt ccttcagttt
    10441 tactataaga gaaacgttaa gttttataaa gataaactta aatctatcta taagtaggag
    10501 atatggtgaa aaactataaa ggttttcgcg gttttgttaa gcgtttatcg tataagtatg
    10561 ataaatctca agaagttgat gcaacaaaag aagtagcact acattggtgg aatggacgag
    10621 ttaattttgg tgatgtcgta aataagttta tcgtagagca gttgtcaggg aagagcgcta
    10681 tttggtcatc cgataaatcg gataaagaac attatttagt cattggttct gtcttacaat
    10741 cctcgaacga taatgcgatt gtttggggaa gtggtattat ttcggataga aaaacgccac
    10801 ttttcaaacc taaaaaagtg catgcagttc gtggtcctaa aaccagagat atcttaatcg
    10861 ctcgaggaat tgaatgccca gaaatttatg gtgatcctgc tttagttctt ccgacattaa
    10921 tacagcccaa cgaagcagag tcagcataca agttaggtat tattccgcat tacaacaata
    10981 agaacgacgc gttcttcaag caaaattttc ctgatgatgt aaaggtcatt gatattgaaa
    11041 ctgatgatgt acaggcattt attgatgata ttgcatcttg tgagctaatc gtttcaagct
    11101 cgttacacgg aattattatt gctgacgctt atggcgtgcc cgctcaccat atttcttttg
    11161 atgacacggt ggaaggagga gagtttaaat tcttcgatta ctacctttca gtggggcgag
    11221 agtgtaattt acctatcaaa gtatcgtctt ccactagtat cgatgagctc tgtgagttgc
    11281 caaagaacta tgacataaat atagatattc aacctctttt agatagctgt ccttttaaac
    11341 aataagatat gccccgaaac taaaatatcg gggcatgttt ttcgtcattt atctcggtga
    11401 actacaagcc tgtcttgaat ttgttcgtag agtctaaaaa gttcaatgta tatcttattg
    11461 cagaggttta tatcagattt atcttttcga gaactgccaa ttgttgatgg aatatcggct
    11521 cttttgacaa tatctggtct taaaacatac gtggatacac catgtttata cggtttttcc
    11581 atgtagtcat ctacaggttc tataaactga gttgcgtttt ttatgaattt cgaagctgca
    11641 tgtggagtta gaatatatcc cataataccg catgtacgtt tgttgaagcg tacaatatta
    11701 agtttgttat taactgaaga tatgacttta aatgatttag gcttggttgc tgcaagcttt
    11761 agaaagtcgt attgttccgc taatgtgtcg aaatgtgaaa gcacgtcaaa aaactccggc
    11821 aataagtcgc agttatcttc taaaaccagt attggttcgt ttacctctaa gcacttctcc
    11881 caggctaggt ggtgactaga gaagcaagca atttcgttct caactagcct atagccaaat
    11941 cgttttcgag taagggcatt attgctacgt tcagagtata gaaagtttgg cgtagaggaa
    12001 tctatcgctt ctataaattc gaatggtatg ttggctttgt tgagtgcatt ttccatgcga
    12061 actcttctat caactgagcg tttcaagtta ataacaaaaa ctttcatttt atactctcaa
    12121 tacatctaat tgtcttagtt agcgcaccaa cattatcttt gacaaagcta aggaggttct
    12181 cttgaacgac tctttttgct aacgcatctt gaaacatagc ttcaacctct agactcatct
    12241 cgttaggtga ctcaatcagt ttgataactt tcttatgaag catcttagca accagatctt
    12301 ggaagttgta ataacttggt cctgtgatta cgggtactcc cagggcagct ggctctaaca
    12361 cattgtgtcc accgacttta tctccgatta gactgccacc cataaagcag atgtctgctg
    12421 catcaatcaa tacgagcatt tcacccattg tatcgcctag gtaaatctga gttgtgtttt
    12481 ctgcaggttg cttttctgtg cgacggacag tttcaaagcc ttgtttttta caaagagcaa
    12541 aaacatcgtc aaatcgttcc ggatgccgag gaactaaaat gagtagagca ttgggatgag
    12601 attctaaaat ctgtttatgt gcctctaata cctgttcatc ctcgccttta tgcgtacttg
    12661 ctgcaatcca tactgggcgg tcttttccaa gttgcgctcg aagtgctttg ccttgttgct
    12721 tcacgtgatc tgaaatttga atatcaaatt taatcgagcc cgtgaccgat agcttctctt
    12781 tattgacgcc taagcgttca aatcgctctg catcagaatc cgtttgacaa agtacttgtg
    12841 tcaagcatgg atgaagttgg ttaaaaagcc attggacttt tgcgtaattt tgacaggatt
    12901 tttctgatag cctagcgtta actacggtga tcggaatatt ggcttgtttg acgacgttga
    12961 gcgtatttgg ccacagttct gtctctataa tcagcatttt ctttggctgt attgccttta
    13021 ggaagctttt tacagcaaag gcaaagtcta ttggcatata gcggtgttca actaaatcac
    13081 ccaacttggc tatttgttcc gcaccagtgc tcgtggttgt tgtgacaacg ataggttgct
    13141 ctggatattg ctgtttgagt tccttgatta gaggtgttgc cgcgatgctt tcgcctacag
    13201 aaacagcatg aatccaaatt ggacgctgat gcgtttttaa ctgtggggtt attccaaagt
    13261 gttctttcca tcgcccacca aatttgggtt tattgggttt gcttttatat aagcccaata
    13321 aaagaaatgg tgaggcaagc gctaacagtg ccgtataaat aattcgaatt aacatgaact
    13381 agtcgcaatg gcttcgagct tctggatact atctaggact tgttttggcg atagctcagt
    13441 caagcatttc aaatgttggt attggcactc acgcttgaaa catggtcggc agtcgatatc
    13501 ggtatgaacc atttcaactt tttcagccag tggtggtgtg tactttggtg atgttgagcc
    13561 ataaacagca acgacattac aacctacagc ggctgctaca tgcattaagc ctgagtcatt
    13621 tgcgaccacc gttttacatg cagcaagaag atcgaccgct tcaattaaac tggtttgccc
    13681 tgctagaaca tgaatgtgtt catggaactg tgttggaaca agtgcacgga tattgttaca
    13741 agtctctagg tctttttgag agccaaatag ccatacttga tggccggctt tgcacatctg
    13801 tgtagcaact tcggcatagt gtgtttccgg ccactttttc gctgggccaa attctgcacc
    13861 aggacataga ccaacagctg gtcgtttttg atctaaattg aatttattga tggttgtttg
    13921 ctgttcttct ttattgatcg acaggctagg gcgaggcagt gtatccaatc ctccaagaga
    13981 agatgaatca accatttctg atttagagta agcgagagcc acataacgct caaccatgta
    14041 ttggaaggat ttcatatttg gacgcaggtc attgagaaga ccgtagcgca tctctccttt
    14101 ccatcctgta cgcagaggta tattcgcaaa ccaaggaatc agcgctgatt tcgctgattt
    14161 tggcagaata taagcgtggt cgtattgctt ttcacgtaat gatttaccaa tctctctgcg
    14221 gcctaataaa ttaaactcac catggcctat cggcatctcg attgcttggt ggatttctgg
    14281 catacgctca agaataggct tacaccagcc gggtgcaata acatcaattt ggctttccgg
    14341 gtgcagttgc tttagtgtga tgtaaagaga ttgggacatc accatatcac caacccatgc
    14401 tgggccaata attagaattt ttttcatttt tcaacttgcc agtcagtgtt gtatctcggc
    14461 ttaaagagat tgtaataatt gttgccgtct tcttgtgaat gcaatagctg tagcacccac
    14521 atatattgtt ctggtttatc accgacgaag tattcaatag cttcgttaca ggtgcgggcg
    14581 tcttgttctt cacctttgtc taaatcaaat gcaggcaaaa tttcgatatc gaatgtgcca
    14641 tcttcggtgt tcattgaagc gaacagaggt aatactttgg aacgtgatag tttagcaatt
    14701 ttacccaatc cagggagcgt tgctttacgt gttgcaaaaa aatcgacaga tacactatgc
    14761 tctgggccgt gatcttgatc gggtaggtaa taccctatat agccatctcg aatggatttc
    14821 agaaaaggct taatgccacc tgagcgttca taaacacgac caccgtattg aactctttgc
    14881 ttgtgcatca gccagtcagc aacatcattc ttttgttttt ttgccatcgc agagacaggc
    14941 atccccattg aagccagcaa aatagcagga acatcgatag cccacgagtg aggcaccatc
    15001 aaaatcacat tttcattgtt atcaatgtgc tggcgtagta cgtccaatcc tcgaatttgg
    15061 cagcgactct gaagccattg aggagaacgc aaactcagca acccaaaacg aaaaaagaac
    15121 atagctgcgg tgtagagcgt tttttcaatt aaggcttcgc gttcctgaaa cgtctttcca
    15181 gggaagcata attcaaagtt gactcgagcc ctgtgagctg gccctttatg agatttagat
    15241 aactttttag ctattctttt ggctagccac ttttgggcgc taagaggcat taaggctagc
    15301 ggcaaaaaga gtattacacc taaccaagtt ccccaatact taggcgacaa aaaatgacgc
    15361 tcaaattcag ggttatatgc tttggagtcg tagtcgtcac gttttgctgt catggcgtgt
    15421 ctagttacct atcctcttga ttactcgtat gagagcaagg tgaaaaataa gaagtagagt
    15481 gatacaggct attggatgga taaaggggaa caatgtgcta ccacctaatg tgtactgttt
    15541 ggaagtaata tcggaacgct cacaccgctt gatcactcga ccggacggac tgtaggctgt
    15601 tgggatctgt aggacttaag ttggaaggat attcatcccc ttctgaggca tgcgtttgtc
    15661 tct
//
