LOCUS       JQ863077               15387 bp    DNA     linear   BCT 11-NOV-2012
DEFINITION  Vibrio parahaemolyticus strain 20579 serogroup O9 O serogroup
            genetic determinant region genomic sequence.
ACCESSION   JQ863077
VERSION     JQ863077.1
KEYWORDS    .
SOURCE      Vibrio parahaemolyticus
  ORGANISM  Vibrio parahaemolyticus
            Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
            Vibrionaceae; Vibrio.
REFERENCE   1  (bases 1 to 15387)
  AUTHORS   Chen,M., Guo,D., Wong,H.C., Zhang,X., Liu,F., Chen,H., Chen,M.,
            Liu,B., Wang,L., Wu,F. and Feng,L.
  TITLE     Development of O-serogroup specific PCR assay for detection and
            identification of Vibrio parahaemolyticus
  JOURNAL   Int. J. Food Microbiol. 159 (2), 122-129 (2012)
   PUBMED   23072697
REFERENCE   2  (bases 1 to 15387)
  AUTHORS   Chen,M., Guo,D., Wong,H.C., Zhang,X., Liu,F., Chen,H., Chen,M.,
            Liu,B., Wang,L., Wu,F. and Feng,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2012) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..15387
                     /organism="Vibrio parahaemolyticus"
                     /mol_type="genomic DNA"
                     /strain="20579"
                     /db_xref="taxon:670"
                     /note="serogroup: O9"
     misc_feature    1..15387
                     /note="O serogroup genetic determinant region"
     gene            454..1164
                     /gene="kdkA"
     CDS             454..1164
                     /gene="kdkA"
                     /note="KdkA"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="AFV92970.1"
                     /translation="MIQQYKNNNQVIWFDDEIITDPSQPIFDAEYWQSSDKVVGSATG
                     RGTTWFIQLENMQAALRHYRRGGLFGKLVNDHYWFSGWDNTRCAQEFQLLLALIEAGV
                     HVPRPIAARAVKTGLFYQADLLSERIPNARDLVSILQEQSLSVEMYQKIGQEIAKMHQ
                     IGVNHTDLNIPNILIDDKDKVWIIDFDKCRFETSGDWKHQHLNRLLRSFNKERLKRQI
                     IWQESDFEHFQDGYASQS"
     gene            1309..2373
                     /gene="rmlB"
     CDS             1309..2373
                     /gene="rmlB"
                     /note="RmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-D-glucose 4,6-dehydratase"
                     /protein_id="AFV92971.1"
                     /translation="MKILVTGGAGFIGSAVARHIIRDTQDTVINLDKLTYAGNLESLV
                     DVSESERYYFEQVDICDRAELDRVFSEHQPDRVMHLAAESHVDRSIDGPAAFIETNVM
                     GTYHLLEAARQYWSSLDEARQSAFRFHHISTDEVYGDLEGTDDLFTETTSYAPSSPYS
                     ASKASSDHLVRAWQRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGD
                     GMQIRDWLFVEDHARALYKVVNEGEVGETYNIGGHNEKANIEVVKTICALLEELRPDK
                     PAGVESYESLITYVKDRPGHDVRYAIDATKIAQELNWTPEETFESGIRKTVEWYLNNP
                     QWWQRVLDGSYSLERLGAGE"
     gene            2373..3245
                     /gene="rmlA"
     CDS             2373..3245
                     /gene="rmlA"
                     /note="RmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AFV92972.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIREILIITTPEDQEGFQRLLGDGSEFGIELQYAIQPSPDGLAQAFIIGEDFIGDDSV
                     CLVLGDNIFYGQGFTPILQQAAMKTRGATVFGYQVKDPERFGVVEFDANMQAISIEEK
                     PVKPKSNYAVTGLYFYDNRVVELAKKVKPSARGELEISTLNQMYLEQGDLNVQLLGRG
                     FAWLDTGTHESLHEASSFVQTIENVQGLKVACLEEIAWRNGWLTRDQLLFIAKPMQKN
                     DYGRYLSELVTEQN"
     gene            3276..4361
                     /gene="wvaD"
     CDS             3276..4361
                     /gene="wvaD"
                     /note="WvaD"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase"
                     /protein_id="AFV92973.1"
                     /translation="MMSNEIVPVTKPYLPSKDKYKQYVDRVYESGWLTNSGPLVKDLE
                     SRLAEYLGVKHVILVANGSLALQVAYKALNLAGEVVTTPFSFAATTSTLNWENLTPIF
                     CDIESDTFNINPDLIEDLITEKTSAIVPVHVFGNPCKVNEIQAIANKHGLKVIYDAAH
                     AFGVDLNKESVLTYGDISTLSFHATKLFHTIEGGAVITQCDELAAKVRSMINFGITGP
                     DSIEGVGTNAKMNEFEAAMGHCVLDDIESIMTKRRGLWKSYQTHLSDVLQMQYWADSS
                     KPNGAYVPIVFDSEQSLLRAKANLEANNIFPRRYFYPSLDLVDSYGHEGTPCEVSRSI
                     SKRILCLPIYFDLCESMINKIKQILKK"
     gene            4365..5924
                     /gene="wvdA"
     CDS             4365..5924
                     /gene="wvdA"
                     /note="WvdA"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase and ligase"
                     /protein_id="AFV92974.1"
                     /translation="MVAKRESKMRNSILKYLQASSMKHPENICVSDSSHSLTYAESTA
                     KSYAISLQLRQLGSLNKPIVVFAKKSVNALSMIFSATMAGGVYCPVDINSPKERLLKI
                     LSNLQDCLVLYDETSRNLLSSLELDSPISTINIDEIPASKLPMQDIWADIEKFNQTVI
                     DKDPCYIIFTSGSTGTPKGVTICHSSVIDYIDWANSAYEVSKADIIGSQAPLFFDNST
                     LDIYLALSNAAELHVIPESVFIFPQKTLEYLKEKAITTIFWVPSLLVNIANLKLLDQF
                     QLPCLKNVLFAGEVMPAKTIKYWLDKHPNAHYSNLYGPTEITVDCTYYDVPSDWDGDS
                     LPIGIPCHNSGILILDENDQRNDEGELCVRGSSLALGYWANSEKTEEVFCQNPLQDKY
                     IDMIYRTGDLVRLVDGLIYYIGRKDFQIKHNGYRIELGEIESQIMDLDAVEQCVVGYL
                     SEDKVLYSFVQCSSSMSEVELKVALAKRLPKYMIPSKVGFVQEMLFTPNGKLNRKAFS
                     EKALQEFVKVK"
     gene            6160..7227
                     /gene="wvdB"
     CDS             6160..7227
                     /gene="wvdB"
                     /note="WvdB"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase"
                     /protein_id="AFV92975.1"
                     /translation="MKSNIGNIALKGILAAVPKHISYFDDEIENYSHTEQSSKKLKKL
                     MGYEQHRVVSKPICFSEMVKPLLENLPKVLGVSYDDIDAIVVVTQTPDYLIPSTSSII
                     HGMFDFNEDCYCIDINDGCNGYIRGLFESSSIIRNSDAKKILLITGDVLSLKVSKKDR
                     NSYPLVGDAIVVTLVEYKDTVDRAPLEIHHDGQGAMALNIPVGGLAIPYQEGSSTLEE
                     DEEGNLRSLEHLVMQGRDVFTFTQTKALSFLKRFTSDHATENPVDYFFLHQANEFILQ
                     RFRTGLQLDSNQLPSEVIKKYGNSSSATIPLSIAEAWQGAHTFVGKRAILAGFGVGLS
                     WGAAICDLSELSVCKIIELEL"
     gene            7439..8179
                     /gene="wvdC"
     CDS             7439..8179
                     /gene="wvdC"
                     /note="WvdC"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="AFV92976.1"
                     /translation="MAKYILITGATSDIGKSVAKRLIESDCVPILIGRNIESLKKAID
                     ELDNPNIRYYLFDNNELSDVKDLFKTIVNDIGAKLSGFIGCAGFHKIKPFRVTKEQDF
                     KEMMQLNFVSQAMFAHCFAKSQFSHDGSSILFISSIASISGEPGLSAYSSSKAALNGL
                     MRTLAVELAPRGITCNSILPGWVQTKHAEFVESTMSSEKFTELRASYPLGLGKPGDVA
                     SLCNFLISEESRWLTGQEIVLDGGKSLL"
     gene            8128..8745
                     /gene="wvdH"
     CDS             8128..8745
                     /gene="wvdH"
                     /note="WvdH"
                     /codon_start=1
                     /transl_table=11
                     /product="GCN5-related N-acetyltransferase"
                     /protein_id="AFV92977.1"
                     /translation="MVNRTGDCTRWWKEFIVIEFKPLEQLNDNEIEVVAKSVCQLDPL
                     FYMQLSQDESECIAKVYRLIITSGTDLCVGDAAFYHGEFVGYSCYFPAKEKKFRSATS
                     LKILLDNKSAIEKKKIISLATEFSKRVAPLETSGLYLNKIHAFKTNVGIGTALLNRFI
                     ERASSGNTPCSLHVNKENEHAILFYQNNGFNMISSGSDYILMTRG"
     gene            8748..9374
                     /gene="wvdE"
     CDS             8748..9374
                     /gene="wvdE"
                     /note="WvdE"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acyltransferase, sialic acid
                     O-acetyltransferase NeuD family"
                     /protein_id="AFV92978.1"
                     /translation="MSLYILGAGGLAKEVYGWLVAEQSPMLEELRGFLVTDSNYSSIE
                     SYDGVPVKVLDSIAEEKGFEFLVCVGTSLPRKKVIEQALMHGGTPKSFVSNNALIGQN
                     VVIGEGSIINPRCTISSDVKIGKYTIVNCSNGIGHDCVVGDYVTVLGNAAINGHVFIG
                     DYVTIGSGAILHPNIKVGDYASVGIGSVVIRNVKPNTTVFGNPARKIS"
     gene            9455..10315
                     /gene="wvdF"
     CDS             9455..10315
                     /gene="wvdF"
                     /note="WvdF"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFV92979.1"
                     /translation="MSTKVSVICTCYNHEKYIEQTLLSILNQTVDFEYELLIHDDCSS
                     DNSRKVIDKVFNEYSGKCQVKRIYPEENIYSKGSSAVLFNMLPHVDGEYIAFCEGDDY
                     WVDDNKLQKQLKALEYHKDINVCFHPSLTLLKGDLVDKKYGYQGDLEKKISYQYVIKK
                     GGDTMPMASMFIRKEPFFDLIYQHEDFFKLYGEHSVLQIINSIEHGAWYLPEIMSVYR
                     SMHEGSWSLQQAQDPAAEEREFTYYKIRILGLNKIYNYKYWKCFLVIYLKKSKKYYRK
                     KIKRWLTAKV"
     gene            complement(10345..11613)
                     /gene="kdtA"
     CDS             complement(10345..11613)
                     /gene="kdtA"
                     /note="KdtA"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="AFV92980.1"
                     /translation="MLVRIVYTLLLALASPLLLFGLYKSKPNKPKFGSRWKEHFGITP
                     KLKSNDKPIWIHAVSVGESIAATPLIKALKEQNPEQSILVTTTTSTGAEQIAKLGDLI
                     EHRYMPIDFGFAIKGFLKAMQPKQMLIIETELWPNTLHNVHKAGIPITVVNARLSEKS
                     CQNYAKIQRLFNQIHPCLTQVLCQTDSDAERFERLGVEKKKLSVTGSIKFDIQISEQV
                     KQQGQQLRAQLGKDRPIWIAASTHKGEDEQVLDAHRQVLKSHPNALLILVPRHPERFD
                     DVFTLCQQQGFKTVRRTSTHAVETNTQVYLGDTMGEMLTLIGAADICFMGGSLIGDKV
                     GGHNVLEPAALGVPIINGPSFFNFKEIVHEMKQNNLIKIVKNEDELARAIVALIKDVA
                     AHERITSELAHFMLANSGSLQRTTTATSSI"
     gene            complement(11607..12662)
                     /gene="waaF"
     CDS             complement(11607..12662)
                     /gene="waaF"
                     /note="WaaF"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose-LPS heptosyltransferase II"
                     /protein_id="AFV92981.1"
                     /translation="MKILVIGPAWVGDMVIPQSLYIVLKQRHPDSQIDVIAPGWCKPI
                     LERMPEIHQAIEMPIGHGEFNLLGRREIGKSLREKQYDHAYILPKSAKSALIPWFANI
                     PLRTGWKGEMRYGLLNDLRPNMKSFQYMVERYVALAYPKAEMVDSNSLCGLETLPRPR
                     LAINAAEQQATIKKFNLAQGQPIIGLCPGAEFGPAKKWPETHYAEVATQMARSGHQIW
                     LFGSQKDLETCNNIRALVPTQFHEHIHVLAGQTSLIEAVDLLAACKTVVANDSGLMHV
                     AAAVGCNVVAVYGSTSPKYTPPLAEKVEIVHTDIDCRPCFQRECQYKHLKCLTELSPK
                     QVLDSIQKLDAITISSC"
     gene            complement(12659..13381)
                     /gene="wvaH"
     CDS             complement(12659..13381)
                     /gene="wvaH"
                     /note="WvaH"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFV92982.1"
                     /translation="MKVFVVSLARSLDRRERIEEKLKQQGITFEFFDAVDGSVDRFLH
                     SEKSRPTITLRRKGYQLKTSEVACFASHYELWVRCVELDEPIVILEDNVDPVEGLQQV
                     LEYTLPLTSNYDYIKLSATQKRVFTPIVPIDDNHMLGGYSAGTCGTTAYIVTPKAASK
                     FVMHAQKFIEPVDDYMEKPWRHGVQVYSVSPDLFTRAEIASTIGGKRKDKSKMTLANK
                     IYAELFRTYESILKFLYWKNKA"
     gene            complement(13430..14074)
                     /gene="msbB"
     CDS             complement(13430..14074)
                     /gene="msbB"
                     /note="MsbB"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
                     acyltransferase"
                     /protein_id="AFV92983.1"
                     /translation="MFFFRFGLLSLRSPKWLQNRCQIRGLDILRQHIDNNENVILMVP
                     HSWAIDVPAILLASMGMPVSAMAKKQKNDVGDWLMHKQRIQYGGRVYERSGGIKPFLK
                     SIRDGYVGYYLPDQDHGPEHSVFVDFFATRKATLPGLGKIAKLSRSKVLPLFASMNTE
                     DGTFDMRFYQRLSWIKAKSKTLVPVMKPIEYFVGDKPEQYMWVLQFAALSRRRQ"
BASE COUNT     4459 a   2891 c   3433 g   4604 t
ORIGIN
        1 ggctaccgtt taccgctgca gttctgcatg tggtgttgtc aaagaccaac cgcatcaatc
       61 gcaagaatct ggcaagccgg gtaacaccgg gctaaagcgc atcgttaaag cgacgggtta
      121 ttcgatccaa ggattaaaag cggcttttaa gcatgaagct gccattcgac aagagttggc
      181 tctgttgagt attgctgtga ttttggcttg gctagtggat gtcggcatgg ttgagcgtat
      241 cttaatgatc ggggtcgtgg tattagtctt gattgtagag cttgtaaact cagcaattga
      301 ggcggtagtg gatcgtatcg gtattgaacg gcatgaactc agtggtcgag ctaaagatat
      361 aggctctgct gccgttatgg ttgctttggc gtttgctgca tttacttggt tgtatatatt
      421 agccagccgg tatttaggct agagggtgag cgtatgattc aacagtataa aaacaacaac
      481 caagtgatat ggtttgatga cgagatcatt acagacccta gccaaccgat atttgatgcg
      541 gaatactggc agtcctccga caaggtggtt ggcagtgcga cggggcgagg cacgacatgg
      601 tttatccaac tagagaacat gcaagcggca ctacgtcatt accgtcgtgg cggtttgttt
      661 gggaagttag ttaacgatca ttattggttc tctggctggg ataacacccg ttgtgcgcaa
      721 gagtttcagc ttttgttggc gctaattgag gctggggtgc acgttcctcg tcctattgca
      781 gcaagagccg taaagacagg actcttctat caagccgatt tattaagtga gcgaatccca
      841 aacgccagag atctggttag tatcttgcaa gaacagtctt tatcggtaga aatgtatcag
      901 aaaatagggc aagaaattgc caagatgcat cagattggcg ttaaccatac cgatttgaat
      961 atccccaata tcttgatcga cgacaaagat aaggtatgga ttatcgattt tgataagtgt
     1021 cgttttgaaa cttctggtga ctggaagcat caacacctaa atagacttct gcgttcattt
     1081 aataaagagc gtttaaagcg acagattatt tggcaagaat ccgattttga acattttcag
     1141 gacggttatg cttctcaatc ttaattgagt tttttatcat tttatattga ttgctctaag
     1201 taagccttct tgagtctaac gatgctcgag tgagtctata aactacaata atcataaagt
     1261 attcatgtgt atctgatggc acaaaaatta acgtaggtaa catttgtaat gaaaatatta
     1321 gtaaccggtg gagcaggctt cattgggtca gctgttgccc gacatatcat tcgtgacacc
     1381 caagataccg tcatcaattt agataaactg acctacgcag ggaatctaga atcactggtt
     1441 gatgtctcag agagtgaacg ttactacttt gagcaagtag atatctgtga ccgagctgaa
     1501 ctagatcgcg tgttttcaga gcatcaaccc gatagggtga tgcatttggc cgccgagtct
     1561 cacgtcgatc gctctattga tggtcctgct gcttttattg agaccaacgt gatgggcact
     1621 taccatttgc ttgaagcggc tcgtcaatat tggtcgagct tagatgaagc gcgccaatcg
     1681 gcattccgtt ttcatcatat ttctacggat gaagtgtatg gcgatctaga gggaacggat
     1741 gatttgttca ccgaaaccac ctcatacgcg ccgagcagcc catattctgc atctaaagct
     1801 tcgagcgatc acctggttcg cgcttggcaa cggacttatg gcttaccaac acttatcact
     1861 aattgctcca acaactatgg tccgtaccac ttcccagaga agttgatccc tctgatgatt
     1921 ttaaatgccc tcgatggcaa gccgttgcct gtgtatggtg atggtatgca gatccgggat
     1981 tggctgtttg tggaagacca tgctcgcgcg ctgtacaaag tggtcaacga aggtgaagtc
     2041 ggtgaaacct acaacatcgg tggtcataac gaaaaagcca acatcgaagt agtgaaaacc
     2101 atttgtgctt tgcttgaaga gctgcgacca gacaagccgg caggtgttga gtcttatgaa
     2161 tctttgatca cttatgtcaa agatcgtcct ggtcatgatg tacgttacgc aattgatgcc
     2221 accaagatcg ctcaagagct taactggacg ccagaagaaa cctttgaaag cggcattcgt
     2281 aaaaccgtgg aatggtacct aaacaacccg caatggtggc aacgcgtgct tgatggttca
     2341 tacagtttag agcgattagg agcgggcgaa taatgaaagg cattattttg gcaggtggct
     2401 cgggcactcg cctttaccct attacacgcg gtgtctctaa gcagttactg ccggtttatg
     2461 acaaaccaat gatttactac ccgttgtcgg tactcatgct tgctggcatt cgtgaaatct
     2521 tgatcatcac cacgccagaa gaccaagaag gatttcagcg cttgttaggg gatggctcag
     2581 agtttggcat tgagttgcaa tacgcgattc aaccaagccc agatggctta gctcaagcct
     2641 tcatcattgg tgaagacttc attggcgatg actcagtatg tttggtgcta ggtgacaaca
     2701 tcttttatgg gcaagggttc acacctatcc ttcagcaagc cgcgatgaag acccgtggtg
     2761 cgacggtatt cggttatcag gttaaagatc ctgagagatt tggcgttgta gaatttgatg
     2821 ccaatatgca ggcgatttcg attgaagaaa aaccagtaaa gcctaaatcc aattatgctg
     2881 tcactgggct gtatttttat gataaccgag tcgtcgaatt ggcaaaaaaa gtcaaacctt
     2941 cagctcgtgg tgaattagag atttcgacgc taaaccaaat gtaccttgag caaggtgacc
     3001 tgaatgttca attactaggg cgcggttttg cttggttaga tactggaacg catgaaagcc
     3061 tacatgaagc ttcttcgttt gttcaaacca ttgaaaatgt tcaaggtctt aaagttgcct
     3121 gtttagaaga gattgcatgg cgcaatgggt ggttaacaag ggaccagcta ctattcattg
     3181 caaaaccgat gcaaaaaaat gattacggac gttacctttc cgagttagtc accgaacaaa
     3241 actagcacta ctcgttagtt gcaatgagat attaaatgat gagcaatgaa attgttcctg
     3301 taacaaaacc atatctaccg tcaaaagata agtataaaca atatgttgat cgtgtgtacg
     3361 agagcggttg gttgaccaat agtggtcctc tcgttaagga tttagaatct cgactagctg
     3421 agtatcttgg ggttaagcat gttatattgg ttgcaaatgg ctcattggca cttcaagtgg
     3481 cttacaaagc actaaatcta gctggagagg tggtcacaac acctttctct ttcgccgcga
     3541 ccacaagcac gcttaattgg gaaaatttaa ctcctatttt ttgtgatatt gaaagtgata
     3601 cctttaatat caatcctgat ttaattgaag atcttattac agaaaaaacg tctgcaatag
     3661 taccggtgca cgtgtttggt aacccatgta aagtcaatga gattcaagct atagcaaaca
     3721 agcacggttt aaaagttatc tatgatgccg ctcatgcttt tggtgtggat ctaaataaag
     3781 aaagtgtatt aacgtacggt gatataagta cattaagttt ccatgccaca aagttattcc
     3841 acacgataga gggtggagct gtgattacac aatgtgatga actagcagct aaagtacgct
     3901 ctatgattaa ctttggaata acgggaccgg attcaataga aggtgttgga accaatgcca
     3961 agatgaatga gtttgaagcc gctatgggtc actgcgttct agatgatata gaaagtatca
     4021 tgaccaagcg aagaggttta tggaaaagtt atcaaactca cctttctgat gttttacaaa
     4081 tgcaatattg ggcggatagc tcaaagccta atggtgcgta cgtaccaatc gtgtttgact
     4141 cggagcagtc attacttaga gctaaggcta acttggaagc gaataacatc tttccaagaa
     4201 ggtactttta tccaagttta gatcttgttg acagttatgg tcatgaagga actccatgtg
     4261 aagttagtcg ctcgattagt aaaaggatct tatgcttgcc tatatatttt gatttgtgtg
     4321 aatcaatgat aaacaagata aaacaaatac ttaaaaaata atgaatggtt gcaaaaagag
     4381 agtctaagat gagaaacagc attctgaaat atctacaagc atcgtcaatg aaacacccag
     4441 aaaatatttg tgtttcggat tcgagccact ctttgactta tgcagaaagc acggcaaagt
     4501 catatgctat ctcattgcaa ctaagacagc tgggaagttt aaacaagccg atagttgtgt
     4561 ttgcgaaaaa atctgtcaat gcactctcca tgatcttttc tgcgacaatg gctggaggtg
     4621 tttactgtcc ggtagacatt aactcaccta aagaacgact attaaagatc ttatctaacc
     4681 ttcaagattg tcttgttctt tatgatgaaa cttcacgaaa tttattaagt tcattagaat
     4741 tagatagtcc aatttcaact attaatatcg atgaaatacc agcatctaag ctacctatgc
     4801 aagatatttg ggctgatatc gaaaagttta atcaaaccgt tattgataaa gatccatgct
     4861 atataatttt tacttcaggc tctacaggca caccaaaagg tgtcactatt tgccattcta
     4921 gtgttattga ttatatagat tgggctaatt cagcctatga agtgagcaag gctgatatta
     4981 ttggtagtca agctccacta ttctttgata actcgacttt agacatctat ttggcgttgt
     5041 ctaatgcggc agaattgcat gttattcctg aaagcgtgtt tatttttcct cagaaaacac
     5101 ttgaatacct gaaagaaaaa gcaataacaa cgatcttctg ggttccttca ttgcttgtaa
     5161 atattgcgaa ccttaagtta cttgatcagt ttcaattgcc atgcttgaag aatgtattgt
     5221 ttgctggaga agtaatgcca gcgaagacga ttaagtactg gttagataag catcctaatg
     5281 ctcattactc taacttgtac ggaccaacag aaattaccgt tgattgcacc tactatgatg
     5341 tgccgtcaga ctgggatggg gatagcttac ctataggaat cccttgccat aactcaggaa
     5401 ttttaatcct agatgaaaac gaccagcgta atgatgaagg tgaattgtgc gttcgaggct
     5461 ctagcttagc gcttgggtat tgggctaact cagaaaaaac agaagaagta ttttgtcaaa
     5521 acccacttca agataaatat attgatatga tctatcgtac cggagacttg gttagacttg
     5581 ttgatggttt aatttattat attggtagaa aagacttcca gattaagcac aatggctatc
     5641 gtatagaact tggcgaaatt gagtctcaga tcatggatct agatgctgtc gaacagtgtg
     5701 ttgttggtta tttatcagaa gataaagtgc tttattcttt tgttcaatgc agtagttcga
     5761 tgagtgaagt tgagcttaaa gttgcactgg cgaaacgttt accaaagtac atgattccaa
     5821 gcaaggttgg ctttgtacaa gaaatgcttt ttacgccaaa tggtaagtta aatagaaaag
     5881 ctttttcaga gaaagcgcta caagaatttg taaaggttaa atagtagtat gtatgaaaaa
     5941 attatagcaa tcctaaccac aatgaaacca gagcacgagt ttactaaaga tactgaactt
     6001 tttgagtcag gtctgttgga ctcttttgat ttgttagtcc tagtaagtga gatcgaagct
     6061 cagctagcta tttcaatccc tggtgaaggt ttggttcctg agaattttgt ttcgccgcat
     6121 gttattggtg agtatcttaa aacgattcag ggttaactta tgaaaagtaa tattggtaat
     6181 atagcattaa agggaatact tgccgcggta cccaagcata tttcttattt cgatgacgag
     6241 atcgaaaatt actctcatac agaacagagc tctaagaaac tgaaaaagtt gatgggttat
     6301 gagcagcatc gcgtagtttc taaaccaatt tgttttagtg agatggtaaa gccgttactt
     6361 gaaaacttac cgaaagtact aggtgtttcg tacgatgata tagatgcaat agttgttgta
     6421 actcagacac ctgactactt gattccatca acctcttcga taatacatgg catgtttgac
     6481 ttcaatgaag attgctattg cattgatatt aatgatggat gtaatggtta tattcgaggt
     6541 ttattcgaat cctctagtat catccgtaat agtgatgcca agaagatatt actgattact
     6601 ggcgatgtac tgagtctaaa agtttcaaag aaagatagaa actcttatcc ccttgttggt
     6661 gatgctatag ttgtgactct tgtagaatat aaagatactg ttgatagagc acctctagag
     6721 atacatcatg acggccaagg tgctatggct ttgaacatcc cagtaggtgg tctggcgata
     6781 ccttaccaag aaggaagttc aactttggaa gaggatgaag aggggaactt acgttctttg
     6841 gagcatttag ttatgcaagg tcgagacgta ttcacattca ctcaaacgaa agcactatct
     6901 ttccttaaac gatttacttc ggatcatgcg actgaaaatc cagtagatta tttttttctg
     6961 catcaagcaa atgagtttat tcttcagcga ttcagaactg gactgcaact tgattcgaac
     7021 caattgcctt ctgaagttat caaaaaatat ggtaattcat catcagcaac gatccctctg
     7081 tctatagctg aagcttggca aggtgcgcac acttttgttg gcaaacgagc tatcttggca
     7141 ggttttggag ttggtctgag ttggggagcc gctatttgtg atttgagtga attatccgta
     7201 tgtaaaataa tagaattgga gttataaatg gagcagttta aagaacactt gattgagata
     7261 ttagacattg agatggaaga attggatatg gattctgagt tctcagaaat ggagtcgtgg
     7321 gactcattag ctcagctatc gtttattgca atggctcaag atgaatatga tgtaaatgta
     7381 tcacacgaac agttaaaaaa tgcaaaaact tttactgatt tacataagtt agttttaaat
     7441 ggctaagtat atcttaatta caggggctac gtccgacatt ggaaaatctg ttgcgaagcg
     7501 gttaattgaa agtgactgtg ttccaatttt aataggaagg aatatcgaat cgctaaaaaa
     7561 agctatcgat gagttagata accctaatat acgttattac ctctttgaca acaacgagtt
     7621 gtcagatgta aaggatttgt ttaaaactat tgttaatgac ataggtgcga aactcagcgg
     7681 ttttattggc tgtgctggtt tccataagat aaagccattt cgagttacaa aagagcagga
     7741 ttttaaggag atgatgcagc taaacttcgt ctcacaagct atgtttgcac attgttttgc
     7801 taagtcacaa ttttctcacg atggaagctc aatactcttt atatcctcta ttgcatcgat
     7861 cagtggagag ccaggtttat ctgcgtattc gtcatcaaaa gcggctctga acgggttaat
     7921 gcgcacttta gcggtcgaat tagctccgag aggtataact tgcaatagta ttctccctgg
     7981 atgggttcag acaaagcatg ctgagtttgt agagtcgacg atgtcatcag agaagttcac
     8041 agaattaagg gcaagctatc cattagggtt agggaagcca ggagatgttg catctctttg
     8101 taacttctta attagcgaag agtcgcgatg gttaacagga caggagattg tactagatgg
     8161 tggaaagagt ttattgtgat tgaatttaag ccacttgagc agttaaatga taatgaaata
     8221 gaagtcgtgg cgaaatctgt ttgccaactt gatcctttgt tctatatgca gctatctcaa
     8281 gatgaatctg agtgtattgc aaaagtttat cgattgatta taacgagcgg aacggatcta
     8341 tgtgttggag atgctgcatt ttatcatggc gagttcgttg gatactcttg ctacttcccc
     8401 gcgaaagaga aaaaatttcg ctctgcgact tccttgaaga tactcctaga caataagtca
     8461 gctattgaga agaaaaagat tatatcttta gccacggagt tttctaaaag agttgcaccg
     8521 cttgagacat ctggtttgta cctaaataaa attcatgctt ttaaaactaa tgttgggata
     8581 ggaactgcgc tactaaatag gtttattgag cgagccagtt cagggaatac cccttgttct
     8641 cttcacgtaa ataaagaaaa cgagcacgcg atactgtttt atcaaaataa tggctttaat
     8701 atgatcagta gtggatccga ctatatttta atgactagag gttgattgtg agcttatata
     8761 ttctgggtgc gggtggatta gctaaggaag tttatggatg gttagtcgct gagcagtcgc
     8821 ctatgttaga ggagcttcga ggttttttag tgaccgactc taattacagt tcaatcgagt
     8881 cttatgatgg tgtaccagta aaagtattag attcgattgc tgaagaaaaa ggttttgagt
     8941 ttttagtttg tgttggaacc tccttaccaa ggaagaaagt tattgaacaa gcattgatgc
     9001 atggtggtac accgaaatcc ttcgtgagca ataatgcact tattggacag aatgttgtca
     9061 taggtgaggg gagtattatt aaccctcgct gtactatatc ttcagatgta aaaataggaa
     9121 agtacactat tgttaactgt agcaatggta ttggtcatga ctgtgttgtc ggtgattatg
     9181 ttacagtatt aggaaacgct gctataaatg gacatgtttt tattggtgat tacgttacta
     9241 tcggctcagg tgcaatatta catcctaata taaaggtagg tgattatgct tcagttggca
     9301 tagggagtgt cgttataagg aatgtcaagc caaatactac tgtatttgga aatccggcaa
     9361 gaaaaattag ttagttatta atgaatatca tccctagaaa tatagctgaa gaaataactc
     9421 tacttaaccg ataaattaga agacagagga aaaaatgtcc actaaagtct cagtcatttg
     9481 tacctgctat aatcacgaga aatatataga gcagacactt ttatctatac ttaatcagac
     9541 ggtagacttt gaatacgagt tgctcataca tgatgattgc tcctctgaca atagtcgaaa
     9601 agttatcgat aaggttttta atgagtattc tggtaaatgc caagtaaaga gaatttatcc
     9661 agaggaaaat atatactcta aaggatcatc tgctgtactt tttaacatgt tacctcatgt
     9721 tgatggggag tatatagctt tttgtgaagg tgatgattat tgggttgatg acaacaagct
     9781 tcaaaaacag cttaaagcat tggaatacca caaggatatt aatgtttgtt ttcatccttc
     9841 tttgactttg ctgaagggtg atttggttga caagaaatac ggataccaag gcgatttaga
     9901 aaagaaaata tcttatcagt atgtcattaa gaagggcgga gacactatgc ctatggcatc
     9961 gatgtttatt agaaaagagc cgttttttga cctaatttat caacatgaag actttttcaa
    10021 gctttatggc gagcactctg tattgcaaat tatcaatagt atcgagcacg gagcttggta
    10081 tctccctgaa ataatgtcag tttatcgtag tatgcatgaa ggctcttgga gtttacaaca
    10141 agcacaagac ccagcggcag aagagcgaga gtttacgtat tataaaatcc gtattttggg
    10201 tttgaacaag atttacaatt ataagtactg gaagtgcttt ttagttatat atttgaaaaa
    10261 gtcgaaaaaa tattaccgaa agaaaataaa gcgttggctt acagctaaag tttaaaaaaa
    10321 cagccgagtt aatactcggc ttttttagat agaagaggtg gctgtggtgg tacgttgtaa
    10381 ggagcctgag tttgctaaca taaaatgagc aagctctgac gtgatgcgct catgagctgc
    10441 gacgtcttta attagagcca cgatagctct tgccagttca tcctcatttt tcacaatttt
    10501 gatgaggttg ttctgcttca tttcatgtac gatttctttg aagttaaaga agcttggacc
    10561 attgataata gggacgccca aagcagcagg ctccaataca ttgtgcccac caaccttatc
    10621 tccaattagg cttccaccca taaagcagat gtccgctgcg ccaatcaacg ttagcatctc
    10681 acccattgta tcgccgagat atacttgagt attcgtctct actgcatgcg ttgaagtacg
    10741 acgcacagtt ttaaatcctt gctgttggca aagcgtgaat acatcatcaa atcgttccgg
    10801 atggcgagga accaaaataa gtaatgcatt tggatgagat tttaaaactt gtctatgtgc
    10861 atctaatacc tgttcgtcct cgcctttgtg ggtgctagct gcaatccaaa ttggacgatc
    10921 ctttccaagc tgggctcgaa gctgctgtcc ttgctgtttc acttgctctg atatttgaat
    10981 atcgaactta atcgagccag taacggatag tttctttttc tcaacgccta agcgttcgaa
    11041 gcgctctgca tctgaatccg tttggcagag tacttgagtt aggcatggat gaatctggtt
    11101 aaaaagccgc tgtattttgg cgtagttctg acacgatttt tcagataggc gcgcattcac
    11161 gacagttata gggatacccg ctttgtgaac attgtgaagc gtgttaggcc acagctcagt
    11221 ctcaatgatc aacatctgtt ttggttgcat tgctttaaga aagcctttga tagcaaaccc
    11281 aaagtcaatt ggcatatagc ggtgttcaat taaatcaccc aacttagcaa tctgttcagc
    11341 tccggtactt gttgttgtag taactagaat agattgttcg gggttttgtt ccttaagggc
    11401 tttaattaaa ggtgttgctg ctatgctttc accaacagat acggcatgta tccaaatagg
    11461 tttgtcgttg ctttttagct tgggagtgat gccaaagtgc tctttccagc gagagccgaa
    11521 cttaggctta tttggtttgc tcttgtacaa accaaataaa agcagcggtg acgctagagc
    11581 gagtagtaag gtataaacaa tacgaactaa catgagctta tcgtaatggc gtcaagtttt
    11641 tggatgctat ctagcacttg cttaggcgaa agttctgtca agcacttaag gtgtttgtat
    11701 tgacattcac gctggaaaca agggcgacaa tcaatatcgg tatgaacaat ctccactttt
    11761 tctgcgagtg ggggggtgta cttcggtgaa gttgagccat acacggcaac aacattgcaa
    11821 ccaaccgcag ctgccacatg cattaagcct gagtcattcg cgacaacagt cttgcatgca
    11881 gcaagcagat cgactgcttc aattaagcta gtttggcctg ctagaacatg aatgtgctca
    11941 tggaattgtg tcggaacaag tgcgcgaata ttgttacaag tctctaagtc tttttgagaa
    12001 ccaaacaacc agatttgatg cccagatcga gccatttgtg tcgcaacttc agcgtagtgt
    12061 gtttcgggcc atttttttgc gggaccaaac tctgctcctg ggcacagtcc gataattggt
    12121 tggccttggg ctaagttgaa ctttttgatc gtggcttgtt gctctgccgc gttgattgca
    12181 aggcgagggc gaggtaaggt ttcaagcccg cagagagagt tagaatccac catttctgct
    12241 tttggataag ctaacgctac atagcgctcg accatgtact gaaaagactt catgttaggg
    12301 cgtaagtcgt tcaataaacc atagcgcatt tctcccttcc agccagtacg aagaggaatg
    12361 ttggcaaacc atgggatcag tgctgatttc gctgacttag gcaatatata ggcgtggtca
    12421 tattgctttt cacgtaatga tttaccaatt tccctgcggc ctaataagtt aaactcacca
    12481 tgaccgatag gcatttcgat cgcttggtgg atttctggca tacgctcaag aatcggttta
    12541 caccatcctg gagcgataac gtcaatttgg ctatctggat ggcgttgctt taatacgata
    12601 tataacgatt gcggaattac catatcgccg acccatgcag ggccaatgac taaaatcttc
    12661 atgctttgtt tttccagtat aggaatttca aaattgactc ataagttcta aaaagttcag
    12721 cgtatatttt attcgcaaga gtcattttgg atttatcttt tcttttacca ccaatcgtcg
    12781 aagcaatctc tgctctagta aagagatctg gagacacgct gtaaacttga acaccatgtc
    12841 tccatggttt ttccatgtag tcatcgactg gttcaataaa cttttgagca tgcataacaa
    12901 actttgaggc tgctttaggt gtgacgatgt aagcggtggt accacaagtg cctgctgagt
    12961 atccccctag catatgatta tcatcaatag gtacgattgg ggtgaataca cgcttctgag
    13021 tggcagacaa cttgatgtag tcataattag aagtcaatgg aagggtatat tcgagtactt
    13081 gctgcagccc ctctacgggg tcgacgttgt cttctaagat gacgatcggt tcatcaagct
    13141 caacacagcg aacccacaac tcgtaatggc ttgcaaaaca ggcgacttca ctggttttta
    13201 attgataccc tttgcggcgt agagtaatgg ttggacgact tttttctgaa tgaagaaaac
    13261 ggtcaacaga gccatctact gcatcaaaaa attcaaacgt aatgccttgt tgttttaact
    13321 tttcttcaat acgttctcgg cgatctagcg atcgagccaa gcttacgaca aatactttca
    13381 ctgtactttc cagtcgctgt tatatcttgg tttaaacaaa ttgtaatagt tattgccgtc
    13441 ttcttgagag tgcagcaaac tgtagtaccc acatatattg ttcgggttta tcaccgacaa
    13501 aatattcaat gggcttcatt acaggtacga gcgtcttgct cttcgccttt atccagctca
    13561 aacgctggta aaatctcata tcaaacgtac catcttcagt gttcatcgac gcaaatagag
    13621 gcagtacttt tgagcgtgat agttttgcaa ttttgcctaa accaggtagc gttgctttac
    13681 gtgttgcaaa aaaatctaca aacacactat gctctgggcc gtgatcttga tcgggtagat
    13741 aataccctac atagccatct cgaatggatt tcagaaaagg cttaatgcca cctgagcgtt
    13801 cataaacacg accaccatat tgaattcttt gcttgtgcat cagccagtca ccaacatcat
    13861 tcttttgttt cttcgccatt gcagaaaccg gcatccccat ggaagccagc aaaatagcag
    13921 gaacatcaat tgcccaagag tggggaacca ttaaaataac attttcgttg ttatcaatgt
    13981 gctgtcgtaa aatgtctagg ccgcgaattt ggcaacggtt ctgaagccat tttggtgagc
    14041 gtaaacttag taacccgaag cgaaaaaaga acatggctgc tgtgtagagt gttttttcta
    14101 tcaaagcttc acgttcttgg aacgactttt ccggaaaaca cagctcaaag ttaaccctcg
    14161 ctcgatgagc tggacctttg tgggatttag acagtttttt ggctatggtt tttggcaacc
    14221 acttttgtaa cccagtggca ttaatgacaa tggtaaaaat aagataacac ctaaccacgt
    14281 gccccagtac ttcggcgact gaaagtggcg cttaaactca gggttatatg ctttaggatc
    14341 gaagtcatca cgttttatgg tcatggcgtg tctagttacc tattcgtgtg cgggcgaagt
    14401 ggtttaaatg agaaaaagat agttaggaat gatacaggct attgcacttg agtaaaggtg
    14461 gcaggttatg gttctaccac cttgtggcgc ttaactgttt tggatagtca agtattcggc
    14521 aacgccttct gcaactgtct tgaattcaac atcacaacct gcggtgcgca gtttggtcag
    14581 atccgcttgt gtgaattctt ggtaagcgcc ttttaggtgc tcagggaatg ggatggtttc
    14641 aatttggcct ttgccatgat gcttgataac tgcttttgct acctcttcaa aagattcagc
    14701 acggccagta ccacagttaa aaatacctga tacgccgttt tctaagaacc acaggtttac
    14761 tttacataca tcaccaacgt aaacgaagtc gcgtttgaat gattcgctgc ccgcgaacaa
    14821 tttaggattt tcacctgcgt taagttggtt gtttagatgg aacgcaactg atgccatgct
    14881 acctttgtgg tcttcacgtg ggccatatac gttgaagtag cggaagccgg tgatttgaga
    14941 aagggtctca ccatgctctt cagcgtcttg ccataggcga cgaacgtagt tatcaaactg
    15001 ctgtttcgag taaccgtata cattcagtgc gccttcgtat tcgcgttctt caacgaaagt
    15061 ctctgtatca ccgtaagtgg ctgcggaaga agcgtatagg aatggaatct cgcgatctaa
    15121 acagtaatgc agcagctctt tagagtactc gtaattgttt agcatcatgt acttaccatc
    15181 ccactcagtc gttgcagagc aagcaccttc gtggaaaatt gcctcaatcg gaccaaaatc
    15241 atcgcccgcc ataatttggg tcagaaagtc gtcacgatcc atgtaatctg tgatgtccag
    15301 atcaactaag ttcttaaact ttttgccatt ttttaggttg tcgacaacca ggatatcgtt
    15361 aataccagct tcgtttagtg cttaaca
//
