LOCUS       HQ396160               14420 bp    DNA     linear   BCT 20-SEP-2011
DEFINITION  Yersinia pseudotuberculosis strain 6088 O-specific polysaccharide
            gene cluster, complete sequence.
ACCESSION   HQ396160
VERSION     HQ396160.1
KEYWORDS    .
SOURCE      Yersinia pseudotuberculosis
  ORGANISM  Yersinia pseudotuberculosis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Yersinia.
REFERENCE   1  (bases 1 to 14420)
  AUTHORS   Kenyon,J.J., De Castro,C., Cunneen,M.M., Reeves,P.R., Molinaro,A.,
            Holst,O. and Skurnik,M.
  TITLE     The genetics and structure of the O-specific polysaccharide of
            Yersinia pseudotuberculosis serotype O:10 and its relationship with
            Escherichia coli O111 and Salmonella enterica O35
  JOURNAL   Glycobiology 21 (9), 1131-1139 (2011)
   PUBMED   21321053
REFERENCE   2  (bases 1 to 14420)
  AUTHORS   Kenyon,J.J., De Castro,C., Cunneen,M.M., Reeves,P.R., Molinaro,A.,
            Holst,O. and Skurnik,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2010) School of Molecular Bioscience, University
            of Sydney, Building G08, Darlington, Sydney, NSW 2006, Australia
FEATURES             Location/Qualifiers
     source          1..14420
                     /organism="Yersinia pseudotuberculosis"
                     /mol_type="genomic DNA"
                     /strain="6088"
                     /serotype="O:10"
                     /db_xref="taxon:633"
     misc_feature    1..14420
                     /note="O-specific polysaccharide gene cluster"
     gene            130..1245
                     /gene="gmd"
     CDS             130..1245
                     /gene="gmd"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="ADX97399.1"
                     /translation="MKKALITGVTGQDGSYLAEFLLEKGYQVHGIKRRSSSFNTQRID
                     HIAQNGKYSSENFILHYGDLTDSSNLTRLLEEIKPDEVYNLGAQSHVAVSFDSPEYTA
                     DVDAMGTLRLLEAIRFLGLEKKTKFYQASTSELYGLVQEIPQKETTPFHPRSPYAVAK
                     LYAYWITVNYREAYGIYACNGILFNHESPRRGETFVTRKITRVLANISQGLESCLHLG
                     NLDSLRDWGHAKDYVRMQWLMLQQDHPEDFVIATGKQISVREFVRMAASELGIELEFK
                     GAGIDEIGIVKSIKNNEVIAVNVGDIIVKVDPKYFRPAEVETLLGDPSKAKNKLGWEP
                     EITVSEMCAEMIKYDLQQAKAIAILKSHGHTVSISIE"
     gene            1293..2189
                     /gene="colB"
     CDS             1293..2189
                     /gene="colB"
                     /codon_start=1
                     /transl_table=11
                     /product="putative GDP-colitose biosynthetic protein B"
                     /protein_id="ADX97400.1"
                     /translation="MVGRNILSHPMSELHDFLIPSSSELNLLDNLQIINYLEEHKPDF
                     IIHAAGVVGGIHANVSNPVKFMVENMSMGLNLITTAKNKNIKKLLNLGSSCMYPKSFD
                     TALSEDLILRGELEPTNEGYALAKIASARLCEYINREDPSFQYKTAIPCNLYGKYDKF
                     DEKNSHMIPAVIRKIAKATEEKKEVVEIWGDGEVRREFMYAEDLADFIFYAINNFTKM
                     PQNINVGLGHDYTITEYYTIIAKTIGYKGGFIYDSTKPVGMRRKLIDNTLLSEFGWNY
                     KTDLKDGISYTYQYFLNENNND"
     gene            2182..3348
                     /gene="colA"
     CDS             2182..3348
                     /gene="colA"
                     /codon_start=1
                     /transl_table=11
                     /product="putative GDP-colitose biosynthetic protein A"
                     /protein_id="ADX97401.1"
                     /translation="MINYPLASSTWDDKELVAIKNIIDSNMFTMGASVKKYEINFANY
                     FGSKYAVMVSSGSTANLLMIASLFFTKNPKLQRGDEVIVPAVSWSTTYFPLQQYGLKV
                     KFVDIDKRTLNIDLKKLESAITDKTKAILAVNLLGNPNDFDAINEIIEGKDIFILEDN
                     CESMGATLNGKQAGTYGLMGTFSSFFSHHIATMEGGCVITDDEELYHILLCIRAHGWT
                     RNLPEFNHVTGQKSSDPFEESFKFVLPGYNVRPLEMSGAIGIEQLKKLPNFIEVRRKN
                     AIVFKKLFYNHPYIDIQYETGESSWFGFALILKEGAPISRTELVKKLVDAGVECRPIV
                     TGNFLKNKEVLKFFDYSIAGDITDAEYIDRHGLFIGNHQIDLTEKIESLHRLLS"
     gene            3429..4727
                     /gene="wzx"
     CDS             3429..4727
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ADX97402.1"
                     /translation="MKIKYSLLIANKKKNQLMISSNSMRFLSNKLIPPIVEQFIGPLA
                     LFILTPIIINNLGTKEYGLWILLLSLSMFSQVICFGVTAWIAKTISECLSISDILKVK
                     RIVRSSLSLIFIIFLPFLFVFIFLYLFDEIDKALIEIIILCIMSCLFQEIDNLFANAT
                     KGFELFDYSLLMEAIGRVVWTGCIVVGILRNDIIFYTCVAIAAKSLIKYIIFSFFVIK
                     IWIIPSYSIYEINSRLIESKWMFLQLLGGTSLSLFDRLLIPIVLGVNKLAAYAPCIQL
                     AQLAFSVPAAANQVLMPMFSRFKSKGNYPPNWLKMVILLSIFSGVPCFLLFIFSQEIL
                     AIWINTSFSNENYIILRVLAVAYGTLSCLSTFHFIMLGIGESKMVAKINLLAGGLTML
                     ATILVSQFGINYIVYMKFIYPIMQFKYLTSIKNFIKNSGQ"
     gene            4727..5770
                     /gene="wzy"
     CDS             4727..5770
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ADX97403.1"
                     /translation="MGKEHVFLIIELSILSFSLILSLVNFYKLKVNLSNFFCMFFIVV
                     ILFSSFIFTKNDYFTGLIKIISFLFLIYFGYVCQRKAALVIILVLLISSMLSYITNWQ
                     LDKYSIFPISFVTLLLASFFDRVGKSKYILMLFILLDLTQGIYTEYRGQLIYNIIVLV
                     FLIFNISMRRRVASIFLVTSLGYICILYFIGYSYFSSYNLIFSPTASNIERSSMIYWS
                     VNHIPDFLFSGPTVELFEANSGDYKSLYSMSQRVPNDPHNFLLMMYIYIGFIPAFFIF
                     IFIFIFIKKGENKYNVEKTNNYMALATFQVILLFSMHPFNSFSRIIVGITIGIFIALG
                     RSNNEIKGKFNDK"
     gene            5760..6593
                     /gene="wbzE"
     CDS             5760..6593
                     /gene="wbzE"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADX97404.1"
                     /translation="MINDLCVSIIIATYNVEKCISRCLDSILKQSYENFEVIIMDGAS
                     TDNSIEIIKSYNDPRLRIFSEPDNGIYDAWNKGLEKSKGNWIAFIGADDKYSSEESLA
                     LLIQAIPLSNNAPVIYGKIENEGPEGNVTGGSGEPWFNLFSFSFNYIKCNLPIPIMSA
                     MYSREFIKDEVFDITLKVTADADLFLRCLQRWNGSPPYFINESKPLVRMGYGGVSTNY
                     RNYIVTLKDSIRTRKKNNISNVNLAIINRIFKVYTLSFIGYMFGDSFISKLLIKYHRI
                     K"
     gene            6605..7732
                     /gene="wbzF"
     CDS             6605..7732
                     /gene="wbzF"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADX97405.1"
                     /translation="MNILYLTTGLSIGGAEKQVVLIANNMLKRGHSVTIISLTGDALV
                     NPVDGVIVHNIGMKKKFFSFISTLLKVRGIIKKLNPDIVHSHMYHANIFSRILRFFVR
                     FPVLICTAHNTNEGGRKRMLMYRMTDNLATLSTNVSEEAVKSFLVKKASFPNRIVCIH
                     NGIDTEKLKFSFFERTKYKKKFNIPLDTPVILSVGRLTKAKDYPNLLDSYFYLIDKNR
                     FDKEPILVIVGAGDLYDELDTYAKTLGIRDKVMFLGARNDVENLINIADVFVLSSEWE
                     GFGLVVAEAMACERLVVATDSGGVKEVIGECGTVVPIKNHELLADGIIAALELPEAVK
                     HRLGQCSRNRIISLFSIEIIVNKWLDLYEKLIEVKYITISK"
     gene            7744..9159
                     /gene="manC"
     CDS             7744..9159
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="ADX97406.1"
                     /translation="MTNSILPVIMAGGSGSRLWPLSRTLYPKQFLSLISSFTMLQDTV
                     ARLEDIEHLSPIFICNQEHRFVVAEQLRENKTLHSGIILEPEGRNTAPAIALAALKAI
                     SYGDDPLLLVLAADHVIENKLEFEKSITEAINYANEGKLVTFGIIPTSPETGYGYIKR
                     GKEINSIVFNVSSFVEKPTIEKATKYVMSGEYYWNSGMFLFKSSVYLETLKKFRPDIY
                     NACFHAMNGSHLDLDFIRINENSFKNCPSESIDYAVMEKSDNSVVVSMNAKWSDIGSW
                     SSLWEVSDKDINGNALRGDVLIDNTNDSYIYSQSRLVAAVGVNNLIIIETKDAILIAD
                     KCKSQDVKRIVEQLKIAKRPEYLQHREVFRPWGKHDTIAEGERYHVKAVTVKPGEKTA
                     TQLHYHRAEHWIVVSGTAKVTKGTDTILLTENESIYIPIGTPHAVENPGSIPLELIEV
                     RSGTYLSENDVIRIEEYGTGY"
     gene            9162..10532
                     /gene="manB"
     CDS             9162..10532
                     /gene="manB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="ADX97407.1"
                     /translation="MKKLTCFKSYDIRGKLDDEINEDVAYRIGRAYGEFEKPRSVVVG
                     GDVRLSSESLKLALSHGLQDAGVDVLDIGLSGTEEIYFSTFHLDVDGGIEVTASHNPI
                     DYNGMKLVRRGARPISGDTGLRDIQKLAELNEFPPVDIEKRGGYKKISIIDEYIDHII
                     GYINPSSFKPLKLVVNSGNGAAGPIIDALERRFSFLDIPITLFKINNNPDGNFPNGIP
                     NPLLPECRQDTSDAVLAYRADMGIAFDGDFDRCFFFDDKGKFIEGYYIVGLLAQLFIQ
                     KEPGSKIIYDPRLCWNTIDIVSAAGGKSVMSKAGHAFIKERMRLEDAIYGGELSAHHY
                     FRDFAYCDSGMIPWLLIAELISIRNKSLANLVNEQIVAYPSSGEINRKVENPLSVINK
                     IKSIYQKNALLIDETDGISMEFECWRFNLRISNTESVIRLNVESRADVNLMMDKTKEI
                     ITMLND"
     gene            10695..11834
                     /gene="wbzG"
     CDS             10695..11834
                     /gene="wbzG"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADX97408.1"
                     /translation="MCIFTRFNMKILYIITKANEIGGAQIHIKDLCIRLLNDGNEPEV
                     IVGENGALVDFLINENIKVHILPSLVRDISPIKDMKCILQMRKLVKEINPDVVTIHSS
                     KAGIIGRLSLICSGLPVIFTAHGWSFANGINDKRKFIYIWIEKFCAIFSDKIITVSKQ
                     DKDLAIKYKVATDEQQIIIHNGILGKSVENKKNDNETCIRLISIARFCEQKDHETLLK
                     ALALINNNNWNLSLIGKGPCLEKTKLLTKELGLNDKVIFLGERLDVEVLLASSDVFLL
                     ISNWEGFPISILEAMRASLPVIASDVGGVCEAVNHGDNGFLVNRKDIYGLSKVIETLI
                     ENKELRLKMGKCGESLFKNDFDFESMYQKTLSVYSDVFNKRKLKG"
     gene            11912..12868
                     /gene="gne"
     CDS             11912..12868
                     /gene="gne"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase"
                     /protein_id="ADX97409.1"
                     /translation="MNIAIIGGSGFIGTNLAKIFIDDNLRFSILDKVKSNVYPEKWVY
                     CDVTDRVSLQEALEGHDIVINLAAEHKDNVRPINLYYDVNVDGAKNICSVAESLNIKT
                     IIFTSSVAVYGFVEKDTDENGVYSPFNNYGKSKLEAEKVYNEWFNNNSMKKLIIIRPT
                     VVFGIGNRGNVYNLFKQITSGKFLMIGSGNNEKSMAYVENVSSFLSHSLSFATGYHLI
                     NYVDKPDFTMNKLTSVIYSCLGKNSKIIRVPYILGVLGGYFFDILAKITGKEYPISSI
                     RIRKFCAKTQFSSKNISNYNFTPRYSLEEAVIKTIENEFIKK"
     gene            12923..14074
                     /gene="wzz"
     CDS             12923..14074
                     /gene="wzz"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant"
                     /protein_id="ADX97410.1"
                     /translation="MNNRFYSNDVSSKNILDDSHATHQIMIKEIDLFELFGVIFSSKL
                     KIILITFVFIIIGLGISYILPQKWTSTAIIALPGDEQVQVLDELITNLTVLDIKVDVS
                     ANYLLSTFKQNFDSQDLREQYLVNTNYFKRLMKNNPEDGLDKRALIERIVNENISSVN
                     PLKDKTEGENEYRYYKLSYSASTPTDARDLLQGSINYVNTIVNADVFRKIQRAVDLAK
                     GIGTDKYSMELLKARNNQKVKIERLRYASSIADAAGVKKPVYSNGSAISDDPDFPITM
                     GSDALNRKLEIEKSVIDLASINTELLNRKLYLDKLNRLEIPNVNIVPFKYLQQPTEPT
                     KRDAPKRALIVILFALVGLMGSVGFVLVEHFVRERKREEEGLKLSQTKE"
BASE COUNT     4799 a   1881 c   2751 g   4989 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tggggtaaag taataaactg aagattcgca
       61 tatattataa aagttaatat gcctctaata tcaacttagg gaggaacata ttaaaatgaa
      121 gaggaatgta tgaaaaaagc actaattaca ggagttactg gtcaagatgg ttcttatttg
      181 gctgagtttc ttttagaaaa aggttatcaa gtacatggta ttaaaaggcg gtcatcgtca
      241 tttaataccc aaagaataga tcatatagct caaaatggaa aatatagcag cgaaaatttt
      301 attttacact acggtgactt aactgactca tcaaatttga ctcggctatt ggaagaaata
      361 aaaccagatg aggtatataa tttaggtgct caatcacatg ttgccgtatc ttttgactcg
      421 ccagaatata cagcagatgt tgatgctatg gggacattac gtttattaga agctattcga
      481 tttttaggat tggagaagaa aactaaattt tatcaagctt ctacttctga actatacggc
      541 ctagtccaag agattcctca gaaagaaaca acaccattcc atccacgctc accctatgcc
      601 gtagcaaaat tatatgcata ctggatcaca gtcaattatc gtgaagccta tggtatatat
      661 gcatgtaatg gcattttatt taatcacgaa tctcctcggc gtggagaaac atttgtaaca
      721 agaaaaatta cacgagtgct tgctaatata tcccaaggtt tagagagttg cttacattta
      781 ggaaacttag actcactacg ggactgggga cacgctaaag attatgtacg catgcagtgg
      841 ttaatgttac agcaagatca ccctgaagat tttgttattg caacaggtaa acaaatttct
      901 gtcagagagt ttgttcgtat ggcagcatca gaattaggta ttgaattgga atttaaaggg
      961 gcaggtatcg atgaaattgg cattgttaaa tcaataaaaa ataatgaggt tatagctgta
     1021 aacgttggtg atattattgt aaaagtagat cctaaatatt tccgacctgc ggaggttgaa
     1081 acactattag gcgatccatc aaaagcgaaa aataaattag gctgggagcc tgaaattact
     1141 gtgagtgaaa tgtgtgctga gatgatcaaa tatgatttac agcaagcaaa agcaatagca
     1201 atattaaaat cacatggcca taccgtttca atttcaatag agtaataaag ttgaggtaaa
     1261 aagtgaaaat acttctcact ggtgcaagag gaatggttgg taggaatatt ctcagccatc
     1321 ctatgtctga attacacgac tttctgatac catcaagcag tgaactaaat ttacttgata
     1381 atttgcagat aataaattat ttagaagaac ataaaccaga ctttattatc catgctgcag
     1441 gtgtggttgg tggtattcat gcaaatgtta gtaatcctgt aaagtttatg gttgaaaata
     1501 tgtcaatggg attgaattta ataactacgg ctaaaaataa aaatataaaa aagttattaa
     1561 atttaggtag ttcctgtatg tatccaaaga gtttcgatac agcattaagt gaagacttga
     1621 ttctaagagg agagttggag ccaacaaatg aaggctatgc tcttgcaaag attgccagcg
     1681 ctcgtttgtg tgaatatata aatagagaag atccgagttt tcagtataaa actgctattc
     1741 catgcaatct ttatggtaaa tatgataagt ttgatgaaaa aaattctcat atgattcctg
     1801 ctgtaataag aaaaattgcc aaagcaactg aagagaaaaa agaggtagtg gaaatttggg
     1861 gggatggtga ggttagacga gaatttatgt atgccgagga tctagctgac tttatatttt
     1921 atgctattaa taattttaca aaaatgcctc aaaatattaa tgtaggacta ggacatgatt
     1981 atacaataac agagtattat acgatcatag ctaaaactat aggatataaa gggggattta
     2041 tttatgatag tactaaacct gtaggtatgc gtcgtaaact tatagacaat acacttcttt
     2101 cagaatttgg ttggaactat aaaacagatt tgaaagatgg gatttcatat acttatcaat
     2161 attttttaaa cgagaacaac aatgattaat tatccattag cgagtagcac ttgggatgac
     2221 aaggagcttg ttgctattaa aaatattata gattctaaca tgtttactat gggagcgtct
     2281 gttaagaaat atgaaataaa tttcgctaat tattttggct caaaatatgc cgtaatggtt
     2341 agttctggct caacagctaa tttattgatg attgcctcat tgttttttac taaaaatccc
     2401 aagctgcaac gaggagatga agttattgtt cctgcggttt cgtggtcaac aacatatttc
     2461 cctttgcaac aatacggact taaagttaaa tttgttgata tagataagag aacgttaaac
     2521 atcgatttga aaaagttaga atcagcgata acagataaaa caaaagctat tttagctgtt
     2581 aatttattag gtaatcctaa tgattttgat gcaattaatg aaatcattga aggtaaagat
     2641 atttttattc tagaagataa ttgtgagtct atgggggcga ctttaaatgg aaaacaagca
     2701 ggtacctatg gtcttatggg aacatttagt tcattttttt cacatcatat tgcgacaatg
     2761 gaaggtggct gcgttatcac tgatgatgaa gaattatacc atatattact ttgtataaga
     2821 gctcatggtt ggactcgtaa tttaccagag ttcaatcatg taactggtca gaaaagtagc
     2881 gatccttttg aggagtcatt caaatttgtt cttcctggat ataatgttcg tcctttagaa
     2941 atgagtggcg caattggtat tgaacaattg aagaaattgc caaattttat tgaagtacgt
     3001 cgaaaaaatg ccattgtatt caaaaaatta ttctataacc atccttacat cgacattcag
     3061 tacgaaacag gcgaaagtag ttggtttgga tttgcgctga tcctaaagga gggggcgcca
     3121 atttctagaa ctgaattagt caaaaaatta gttgatgcag gtgttgaatg tcgtcctatt
     3181 gttactggta attttttaaa aaataaagaa gtattgaagt tttttgacta tagtattgcc
     3241 ggtgatatta cggatgcaga atatatagat agacatgggt tgtttatagg taaccaccaa
     3301 atagacttaa ctgaaaaaat agaatcatta catcgtttgt taagttgaat tgttctatgg
     3361 cctagaatag ggtgtgtttt taatgcatcc tattatttct ttattgattt agtaaataaa
     3421 tatatctctt gaaaattaaa tactctttat tgattgctaa taaaaagaag aaccagttaa
     3481 tgatatctag taattctatg cgttttttat ctaacaaatt aatcccacct attgttgagc
     3541 aattcatagg gccgcttgct ttatttatat tgacaccaat tataattaat aatctcggga
     3601 ctaaagaata tggcttgtgg attttattat tatcattatc tatgttctca caagtgattt
     3661 gttttggtgt gacagcttgg atagccaaaa caatatcaga gtgtttatcc attagcgata
     3721 ttttaaaggt aaaaaggata gttcgttcat cgttatcatt aatttttata atttttcttc
     3781 catttttatt tgtttttata tttttatatt tatttgatga aatagataag gctttaattg
     3841 aaataattat tttatgtata atgtcttgtc tttttcaaga gatagataat ctatttgcaa
     3901 atgcaacgaa agggttcgaa ctatttgatt attcgttatt gatggaagct ataggcagag
     3961 ttgtttggac tggatgcatt gttgttggta tattacgcaa tgatataatt ttttacacct
     4021 gtgttgctat tgccgctaaa tctctaatta aatatataat atttagtttt tttgtaataa
     4081 aaatatggat aatccctagc tattctatat atgaaattaa tagtcgatta attgagtcta
     4141 aatggatgtt tttacagcta ttaggaggta catcactaag tttgtttgat cgattattga
     4201 ttcccatagt tttaggtgtg aacaagttag ctgcttatgc accatgcata caactagctc
     4261 agttggcttt tagtgtacct gctgctgcta atcaggtgct gatgccaatg ttttcaagat
     4321 ttaaatctaa aggaaattat ccgccgaatt ggttaaaaat ggtcatttta ttgtctatct
     4381 tttcgggggt tccttgcttt ttgttattta tatttagtca ggagatttta gctatatgga
     4441 ttaatacatc tttttctaat gaaaattaca ttatcttgag agtattagca gtagcttatg
     4501 gtactctttc atgtttaagt acatttcatt tcataatgtt ggggattggt gaatctaaaa
     4561 tggtagctaa aataaattta ttagccggag gtttaactat gctagctaca atactagtat
     4621 ctcagtttgg catcaactat attgtataca tgaaatttat ttatccaatt atgcaattta
     4681 aatatttaac atcaattaaa aattttataa aaaacagtgg ccagtaatgg ggaaagagca
     4741 tgttttttta atcattgagt tgtctatttt atcattttca ttaatattat cattggtcaa
     4801 tttctataaa ttaaaagtaa atttaagcaa ttttttttgt atgtttttca ttgttgtcat
     4861 actgtttagt agttttattt ttacaaaaaa tgattatttt actggtttga taaaaataat
     4921 ttcattttta tttcttatat actttggata tgtttgtcag aggaaagctg cacttgtaat
     4981 aatattggta ttgctaatta gctcaatgct aagttatatt acaaactggc aattagataa
     5041 gtatagtata tttcctattt ctttcgtcac attattgctg gcatcatttt ttgatcgagt
     5101 tggaaagtca aagtatatat taatgttatt tatactactg gatttgacac aaggaatata
     5161 cacggaatat agaggtcaat taatttacaa tattattgtt cttgtttttt tgatatttaa
     5221 tatttctatg aggcgaagag tagcctcaat atttttagta acctcccttg gttatatttg
     5281 tattttatat tttatcggct attcatattt ctcatcttac aatttaattt tttcaccaac
     5341 tgcaagtaat atagaacgtt ctagtatgat ttattggagt gttaatcaca tccctgattt
     5401 tttatttagt ggtcctacag tcgaactttt tgaagcaaac tcaggagact ataaatcatt
     5461 gtattcaatg agccaacgtg taccaaatga tccacataat tttttattga tgatgtatat
     5521 atacatagga tttattccgg cattttttat ttttattttt atttttattt ttattaaaaa
     5581 aggcgaaaat aaatataatg tagaaaaaac aaataactat atggcattgg ctacttttca
     5641 agtaattctt ttattttcta tgcatccttt taactctttt tcaagaataa ttgtaggtat
     5701 aactataggg atttttattg ctttagggcg aagtaataat gagataaaag gtaaatttaa
     5761 tgataaatga tctatgtgta agcattatta ttgcaactta taatgttgaa aaatgtatat
     5821 ctaggtgtct tgatagtata ttaaaacagt cgtatgaaaa ttttgaagtc ataattatgg
     5881 atggggcttc aaccgataat agtatcgaga ttattaagag ttataatgat cctaggctcc
     5941 gaattttttc ggagccggat aatggaattt atgatgcatg gaataaagga ttagagaaat
     6001 caaaaggaaa ttggatagca tttattgggg ctgatgataa atatagttca gaagaatcat
     6061 tagctttgct tatccaagct ataccgttaa gtaataatgc ccctgtaata tatggtaaaa
     6121 tagaaaatga agggcctgag ggcaatgtca ctggaggaag tggtgaacct tggtttaatc
     6181 ttttttcatt ctcatttaat tacattaaat gtaatctccc catacctata atgagcgcta
     6241 tgtactcaag agagtttatc aaagatgaag tattcgatat tactttgaag gttactgcgg
     6301 atgcggatct ctttttaagg tgtttacaac gatggaatgg ttcgcctcca tattttatta
     6361 atgaaagtaa accactagtg agaatgggat atggcggagt atccacaaat tataggaatt
     6421 atattgtaac attaaaggat tcaattcgga caaggaagaa aaataacata tctaatgtta
     6481 acttggcaat tattaataga atttttaaag tttatacatt gtcttttatt ggttatatgt
     6541 ttggtgattc ttttatttct aaacttttaa taaagtatca tagaataaaa taggagtata
     6601 atgtatgaat atattgtatt taaccaccgg gttaagtatt ggtggggcag aaaagcaagt
     6661 tgttctcatt gctaataata tgctaaaaag agggcatagt gtcaccataa tttctcttac
     6721 tggtgatgct ttagttaatc ctgttgatgg tgttattgtt cataatatcg gtatgaaaaa
     6781 aaagtttttt tcttttattt cgactttatt aaaagtaaga ggaataataa aaaaattaaa
     6841 tccagatatt gttcatagcc atatgtacca tgctaatatt ttttctcgta ttcttagatt
     6901 ttttgtccgt tttcctgttt tgatctgtac ggcccataat actaatgaag gtggcagaaa
     6961 aagaatgtta atgtacagaa tgactgacaa ccttgcgaca ctttcaacta atgttagtga
     7021 agaggctgtt aaatctttct tagttaagaa agcatcgttt ccaaatagaa tagtatgtat
     7081 tcacaatgga atagatactg aaaaactgaa attttcattt tttgaacgaa ctaaatataa
     7141 gaagaaattt aacattcctc ttgatacacc agtaatatta agtgttggcc gtttgactaa
     7201 agcaaaggat tatcctaatt tgcttgattc ttatttttat ttaattgata aaaatcgatt
     7261 tgataaagaa ccaatattag ttattgttgg tgctggtgat ttatatgatg agttagatac
     7321 ctatgctaag acattaggta tacgtgacaa ggttatgttt cttggtgcaa gaaatgatgt
     7381 ggaaaattta attaatattg cagatgtttt tgttttgtct tctgaatggg aaggctttgg
     7441 gttagttgtt gctgaggcta tggcttgtga gcgattagtc gtagcaactg attctggtgg
     7501 agtcaaggag gttattggtg agtgcgggac agttgttcct attaaaaatc atgagttgct
     7561 agctgatggt ataattgctg ctttagaatt accagaggct gttaagcatc ggttaggaca
     7621 atgttcgcgt aatagaatta ttagtctttt ttcaattgaa attatagtca ataagtggtt
     7681 ggatctatat gaaaaattga ttgaagttaa gtatattact atctctaaat agaaaattta
     7741 aatatgacta atagcatact tcctgtaatt atggccggag gttcgggtag tcgtctatgg
     7801 ccgttgtccc gaactttata tcccaaacag tttttatcat taatatcatc atttacaatg
     7861 cttcaggata ctgtagctag actggaggat atagagcatt tatctccaat atttatttgt
     7921 aaccaagagc atcgattcgt agttgcggaa cagttgagag aaaataaaac tttacatagt
     7981 gggataattt tagagccaga agggcgaaat actgcaccag caatagctct ggcggctttg
     8041 aaagctatta gctatggtga tgatccactt ttattagtat tggcagcaga tcatgttatt
     8101 gaaaataaat tggaatttga aaagtctatt actgaagcta ttaattatgc taacgaaggt
     8161 aagttagtta cttttggtat tattccaacg agccctgaaa ctggttatgg ttatataaaa
     8221 agagggaagg agattaatag tatagttttc aatgtgagtt cttttgttga aaaacctact
     8281 attgaaaaag ccactaaata tgtaatgtct ggggaatatt attggaatag tggtatgttt
     8341 ttatttaaat cgtcagtata tctagaaact ctaaaaaaat tcagacctga tatttacaat
     8401 gcttgcttcc atgctatgaa tggctctcat cttgaccttg attttataag gataaatgaa
     8461 aattcattta aaaattgtcc tagtgagtct attgattatg ctgtaatgga aaagtctgat
     8521 aattctgttg ttgtcagcat gaatgccaaa tggagcgata ttggttcatg gtcatcactt
     8581 tgggaagtta gtgataagga tatcaatggt aacgcgttac gaggtgatgt gctcattgat
     8641 aatactaatg atagctatat atattcacag agcaggcttg ttgctgcagt tggtgtaaat
     8701 aatcttatta ttattgaaac aaaagatgcg atccttatag cagataaatg taaatcacaa
     8761 gatgtgaaga ggatcgtcga acaattgaaa atagcaaagc gacccgaata tttacaacat
     8821 agagaggtct ttcgtccatg gggaaaacat gacactattg ctgaaggtga gcgttatcat
     8881 gttaaggctg tcacggttaa acctggcgag aaaactgcaa ctcaacttca ctaccacaga
     8941 gctgaacatt ggatagttgt ttctgggaca gcaaaagtga ctaaaggtac tgatactata
     9001 ttgttaactg aaaatgaatc aatttatatt cctataggca cccctcatgc cgttgaaaat
     9061 ccaggatcta ttccattaga attaattgag gttcgtagcg gtacatactt aagtgaaaat
     9121 gatgtaatac gtattgaaga atatgggact ggttactaat aatgaaaaag ctaacatgtt
     9181 ttaaatcgta cgatattaga gggaaacttg atgatgagat caatgaggat gttgcttatc
     9241 gtataggccg agcatatggt gagtttgaaa aacctagaag tgtagttgtt ggtggtgatg
     9301 ttagattgtc tagtgagagt ttaaaattgg cactttcgca tggtttacaa gatgctggag
     9361 ttgatgtttt ggatattggt ttatccggga cagaggaaat atatttttca acctttcatt
     9421 tagatgttga tggggggatt gaagtaacag ccagccacaa tccaattgat tataatggca
     9481 tgaaattggt tcgtaggggc gctcgaccga ttagtggtga cacagggcta cgtgatatac
     9541 aaaagttggc agaattgaat gaatttccac ctgtagatat agaaaaaaga ggggggtata
     9601 aaaaaatatc tattattgat gaatatattg atcatattat aggatatatt aacccatcat
     9661 catttaaacc attaaagttg gttgtcaata gtgggaatgg agctgctggc ccgatcatag
     9721 atgcattaga aaggcgtttt tcttttttgg atatacctat tactttattt aaaataaata
     9781 ataatcctga tggtaatttt cctaatggta tccctaaccc tcttttacca gagtgtcgtc
     9841 aagatacgtc tgatgctgta ttggcttata gagctgatat gggaatagct tttgatggtg
     9901 attttgatcg ctgtttcttc tttgatgata aaggtaaatt cattgaaggt tattacattg
     9961 taggtctttt agctcaactt tttatacaaa aagaaccagg ttcaaaaata atttatgatc
    10021 ctcgcttatg ctggaataca atagatattg ttagtgcagc tggcgggaaa tctgttatgt
    10081 caaaagcagg acatgctttt attaaagaaa gaatgcgctt agaagatgca atctatggtg
    10141 gggagttgag tgctcatcat tattttcgtg attttgcata ttgtgatagc ggtatgatac
    10201 cgtggctcct tattgcagag ctcatttcaa ttagaaataa gagtttagct aatttagtga
    10261 acgaacaaat tgtggcatat ccatcctcag gtgaaatcaa ccgtaaggta gaaaatccac
    10321 taagtgtaat taataaaata aaatctatat atcaaaaaaa tgccttgtta atcgatgaaa
    10381 cagacggtat tagtatggag tttgaatgtt ggagatttaa tcttaggatt tccaatacag
    10441 agtcagttat tcgattaaat gttgagtcta gagcggatgt taacttaatg atggataaaa
    10501 caaaagaaat aataactatg ctaaatgatt aatgatgttt tttattggtg agagttattt
    10561 ttataaaatg ttgttattgt tttttgagtt tttttttttt tttgcatatt tataatatca
    10621 tgtattaagt tttatttatg tagatttgca tgttgaattt ttataattaa tgtcatttgt
    10681 gtttttaaat tttattgtgt atatttacaa ggtttaatat gaaaatttta tatatcatca
    10741 caaaagccaa tgaaattgga ggggcgcaaa ttcatattaa agacctatgc attaggttgt
    10801 taaatgatgg gaatgagcct gaagtaattg taggtgagaa tggtgcttta gtcgattttt
    10861 taataaatga aaatataaaa gtccatatac taccttcatt agttagagat atatcaccaa
    10921 ttaaagatat gaaatgtata cttcaaatga ggaagttagt aaaagaaata aatcctgatg
    10981 ttgttactat tcactcatca aaagcgggga tcattgggcg acttagcttg atttgtagtg
    11041 ggcttcctgt gatttttact gctcatggat ggtcttttgc aaatggtatt aatgataaaa
    11101 ggaagtttat ttatatttgg attgaaaaat tttgtgctat tttttcggat aaaataatta
    11161 ctgtatcaaa gcaagataaa gatcttgcga taaaatataa agtggcaacc gatgaacagc
    11221 aaattatcat acataatggt attctaggta agtctgtcga aaataaaaaa aatgataatg
    11281 aaacttgcat tagattaata tcaatagcaa ggttttgtga acaaaaagat catgaaacct
    11341 tactgaaagc attagcctta attaataata ataattggaa tctttcttta attggtaaag
    11401 gaccatgttt agagaaaacc aaactactca ctaaagagtt gggacttaat gacaaagtga
    11461 tctttttagg tgaaaggcta gatgttgagg ttcttcttgc atcaagtgat gtttttttac
    11521 ttatatccaa ttgggaagga ttcccaataa gtatattaga agcaatgaga gcatcattac
    11581 ctgttattgc atctgatgtc ggtggcgttt gtgaagctgt taatcatgga gataatggct
    11641 ttctagtaaa tagaaaagat atatatggat tatctaaagt gatcgaaaca cttattgaaa
    11701 acaaagaatt aaggttaaaa atggggaagt gtggagagtc attatttaag aatgattttg
    11761 attttgaatc aatgtaccaa aaaacgctat ctgtctatag tgatgtgttt aataaaagaa
    11821 aattaaaagg ttgaggttaa tgaatttgtc taatttatag ataaattaat tttgaaatat
    11881 tactattaac tgatttatat gaggtgggtt catgaatatt gctattatag gtggctctgg
    11941 ttttattggc actaacctag ctaagatttt tattgatgat aacttgaggt tttctatact
    12001 ggataaagta aaaagcaatg tctacccaga gaagtgggta tattgtgatg tgacagatag
    12061 agtaagcctt caagaagcat tggaaggcca tgatatcgtg ataaatttgg ctgcagaaca
    12121 taaagataat gtccgaccaa taaatctata ttacgatgtg aatgttgatg gagcaaaaaa
    12181 tatttgttct gtcgcagaat ctttaaatat taaaacaatt atatttactt cgtctgttgc
    12241 tgtttatggt tttgttgaaa aagacactga tgaaaatggt gtgtattccc catttaataa
    12301 ttatgggaaa tctaaactag aggcagaaaa agtatataac gaatggttta ataataattc
    12361 aatgaaaaaa cttataatta tacggccaac agtagttttt ggtattggca ataggggaaa
    12421 tgtctataat ttattcaagc aaatcactag tggaaaattt ttaatgatag gctctggtaa
    12481 taatgaaaaa tctatggctt atgttgaaaa tgtttctagt tttctttctc attcattgag
    12541 ttttgctaca ggctatcatt taattaacta tgtagataag ccggatttca caatgaataa
    12601 attaacaagc gttatttact catgtttagg gaaaaatagt aaaataataa gagttcctta
    12661 tattttggga gtcttaggtg gttatttttt tgatattcta gccaaaatta cagggaaaga
    12721 gtatccgatc agtagtataa gaattaggaa gttttgtgct aaaacacagt ttagttctaa
    12781 aaatataagt aattataatt ttacaccaag atactctctt gaagaggcag ttataaagac
    12841 tattgaaaat gaatttatta aaaaataaaa atcttttgat atattgataa gtattttttt
    12901 aatttattag catggagggg tgatgaataa tagattttat tctaatgatg ttagttcaaa
    12961 aaatatattg gatgattctc atgcaactca tcaaataatg ataaaggaaa ttgatctttt
    13021 tgaactgttt ggtgttattt ttagctccaa acttaaaata atattaatta catttgtttt
    13081 tataattatc ggattaggta tttcatatat cctcccccaa aaatggacaa gcactgcaat
    13141 aatagctctt cctggtgatg agcaagttca agttctggat gaactaatca caaatctgac
    13201 cgtgcttgat ataaaggttg atgtgagtgc taattatttg ctgtcaacat tcaaacaaaa
    13261 tttcgattct caagatctcc gtgaacaata tttagtaaat actaattact ttaaacgttt
    13321 gatgaagaat aatccagaag atggtttgga taaaagagcg ttaatagagc gaatcgtaaa
    13381 tgaaaatatt tcttcggtta acccattgaa agataaaacc gagggtgaaa atgaatatcg
    13441 ctattataaa ttatcatata gtgcaagcac accgacagac gctcgtgact tgttgcaagg
    13501 ctctattaac tatgtaaata ccatcgttaa tgctgatgtt ttccgaaaaa tacagcgagc
    13561 agtggattta gccaagggta tcggtacaga taaatactct atggaattgt tgaaagctag
    13621 aaataaccaa aaagttaaaa ttgagcgctt aaggtatgct tcttctatcg ctgatgccgc
    13681 aggtgtaaaa aaaccagttt acagcaatgg ctcagccatt agtgatgatc cagacttccc
    13741 tattactatg ggatccgatg cgctgaaccg taaactggaa atagagaagt cagttatcga
    13801 tttggcttca atcaatactg aacttctaaa ccgtaagttg tatttggata aattaaatag
    13861 gttagaaatt cctaatgtta atattgtgcc atttaaatat ttgcaacagc caacggaacc
    13921 cactaaaaga gatgccccta agcgcgcatt gattgtgatt ctgtttgccc tggtcggtct
    13981 tatgggttct gtcggttttg ttttagttga gcactttgtg cgtgaacgga agcgagaaga
    14041 agaggggctt aagctctctc aaactaagga ataggtttta aacgaattta tctaacggct
    14101 gggtgaggtt tctcatctgg ccgttctgtt ttatcgctct gtattatttt actatttttc
    14161 cgtttttaag ttagctgcta ctatttgctt gtaactaatt cccctgcatt atgcttccca
    14221 gcaaccctta tgctaaaatt cgttatcttt tcatcgctag atttttaact caaaacaatg
    14281 aaatttcctg gtaagcgcaa gtccaaacac tactttccag tcaatgcccg cgatccacta
    14341 ttgcaatcgg tgcaggctga aaatgaagtg agtacctctt atatcgttgg tattgaccga
    14401 cactggttga tattgaggca
//
